V.P.M.G. Rutten

Publications (5)0 Total impact

• Article: Immune responses in dogs with cutaneous adverse food reactions.

  E Z Veenhof, E F Knol, T Willemse, V P M G Rutten
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  ABSTRACT: Adverse food reactions (AFR) in dogs are reactions due to apparently harmless food antigens, with an unknown aetiology, i.e. immunopathogenesis. Despite the entry of food allergens via the intestinal tract, in the majority of dogs with AFR, clinical symptoms are only associated with the skin (CAFR). In the present review, factors are presented of relevance in triggering the differentiation of naive T cells into effector T cell types and the role of these T cell types in allergy. More specifically, the allergic immune responses in intestine and skin are discussed in this article as well as the potential pathways, e.g. homing of antigen presenting cells or allergen-induced T cells to the skin, of induction of cutaneous symptoms.
  The Veterinary quarterly. 08/2012; 32(2):87-98.
• Article: Immunostimulatory oligonucleotides modulate canine peripheral blood mononuclear cell activity in vitro.

  A. Jassies-van der Lee, V.P.M.G. Rutten, E. Hessel, J.A. Wagenaar, W. van Eden, A. Willemse
• Article: A GeNorm algorithm-based selection of reference genes for quantitative real-time PCR in skin biopsies of healthy dogs and dogs with atopic dermatitis

  Y.M. Schlotter, E.Z. Veenhof, B. Brinkhof, V.P.M.G. Rutten, B. Spee, A. Willemse, L.C. Penning
• Article: Enzymes involved in the conversion of arachidonic acid to eicosanoids in the skin of atopic dogs

  Y.M. Schlotter, F.M. Riemers, V.P.M.G. Rutten, E.F. Knol, T Willemse
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  ABSTRACT: Canineatopic dermatitis (AD), a chronic inflammatory skin disease, shares characteristics with its human counterpart. To get insight into the role of enzymes involved in production of prostaglandin E2 (PGE2) and leukotriene B4 (LTB4), potent inflammatory mediators originating from membrane-derived arachidonic acid (AA), expression of genes encoding these enzymes and receptors was quantified by qPCR in non-lesional and lesional skin from atopic dogs and in healthy skin. Significantly higher mRNA expression of the key enzymes 5-lipoxygenase (5-LO), 5-LO activating protein (FLAP), leukotriene A4 hydrolase (LTA4H) and prostaglandin E synthase 1 (mPGES-1) and their receptors (PGE receptors 2 and 3) were observed. Being responsible for elevated levels of metabolites of the 3-series prostaglandins and the 5-series leukotrienes these enzymes may be interesting targets for therapy that should result in amelioration of clinical signs in canine atopic dermatitis
• Article: Evaluation of T cell activation in the duodenum of dogs with cutaneous food hypersensitivity

  E.Z. Veenhof, V.P.M.G. Rutten, R. van Noort, E.F. Knol, T Willemse
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  ABSTRACT: OBJECTIVE: To determine whether skin-related clinical signs in cutaneous food hypersensitivity (CFH) coincide with immune reactivity in the intestine in dogs. ANIMALS: 11 dogs with CFH without intestinal clinical signs and 8 healthy control dogs. PROCEDURES: After a provocation and elimination diet, the duodenal gene expression levels of Th1-, Th2- and Treg-related cytokines and transcription factors were investigated by means of quantitative PCR assay. The presence of CD3(+), CD8(+), CD4(+), CD1c(+), gammadelta T-cell receptor(+), and major histocompatibility complex II(+) cells in duodenal epithelium and lamina propria were determined. RESULTS: The expression of Th1-, Th2-, and Treg-related genes in dogs with CFH and healthy control dogs was similar. Although clinical signs disappeared, there was no effect of the elimination diet on cytokines, transcription factors, or cellular phenotypes. CONCLUSIONS AND CLINICAL RELEVANCE: No change in T-cell phenotypes or a distinct Th1, Th2, or Treg profile was detected in the duodenum of dogs with only cutaneous clinical signs of food hypersensitivity. This suggested that the intestinal mucosa is not the primary site of T-cell activation that eventually leads to cutaneous food hypersensitivity.