ABSTRACT: To construct strains containing green fluorescent protein (GFP) to study gene regulation in Saccharomyces albicans cells during the infection process.
pACT1-GFP was constructed, and Saccharomyces albicans CAI4 was transformed. The expression of GFP in yeast and hyphal compartments was observed with microscopy.
99% of Saccharomyces albicans cells containing pACT1-GFP fusion displayed significant fluorescence levels both in the yeast and hyphal compartments. The fluorescence intensity in two compartments had no obvious difference.
pACT1-GFP can be expressed stably in the yeast cells.
Hua xi kou qiang yi xue za zhi = Huaxi kouqiang yixue zazhi = West China journal of stomatology 04/2012; 30(2):115-8.