Mingchun Wang

Nanjing Agricultural University, Nanjing, Jiangsu Sheng, China

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Publications (7)17.02 Total impact

  • [Show abstract] [Hide abstract]
    ABSTRACT: A polysaccharide fraction (SFPSA) was obtained from the rhizomes of Stachys floridana Schuttl. ex Benth. by hot water extraction and sequential purification of anion-exchange chromatography and gel-filtration chromatography. SFPSA was found to be an acidic polysaccharide fraction with an average molecular weight of 168 kDa and composed of rhamnose, glucuronic acid, galacturonic acid, glucose, galactose and arabinose in a molar percentage of 7.75:1.65:14.92:1.87:33.17:40.64, respectively. SFPSA could inhibit the proliferation and growth of human colon cancer HT-29 cells in a time- and dose-dependent manner within 48 h, induce the apoptosis and increase the accumulation in G2/M phase of HT-29 cells. Furthermore, it was found that SFPSA could decrease Bcl-2 mRNA level, and increase the mRNA expressions of Bax and p53 and the activity of caspase-3. These results indicated that SFPSA might induce apoptosis of HT-29 cells through regulation of apoptosis-associated gene expressions such as Bcl-2, Bax and p53 and the activiation of caspase-3.
    Food and chemical toxicology: an international journal published for the British Industrial Biological Research Association 07/2013; · 2.99 Impact Factor
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    ABSTRACT: The peduncles of Hovenia dulcis, containing abundant nutrients and having a taste like a combination of raisin, clove, cinnamon and sugar, have been consumed as fruits and used as traditional herbal medicine for a long time in China. Up to date, little information is available about the polysaccharides from peduncles of H. dulcis (HDPS) and their potential bioactivity. In this study, three purified fractions were prepared by sequential purification of crude HDPS through ion-exchange chromatography and gel-filtration chromatography. The three fractions of HDPS-1, HDPS-2 and HDPS-3 were found to be homogeneous heteropolysaccharides mainly composed of rhamnose, arabinose, galactose and galacturonic acid with an average molecular weight of 235, 70 and 53kDa, respectively. HDPS-3 was quite different from HDPS-1 and HDPS-2, as it contained much higher content of galacturonic acid (40.5%). In vitro immunostimulatory activity evaluation revealed that all the three fractions could significantly stimulate the proliferation of splenocytes and enhance phagocytosis, nitric oxide production and acid phosphatase activity of peritoneal macrophages, which suggested that HDPS had a potent immunostimulatory activity and could be explored as a potential natural immunomodulatory agent.
    Food Chemistry 05/2013; 138(1):41-7. · 3.33 Impact Factor
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    ABSTRACT: The fresh fleshy peduncles of Hovenia dulcis have been used as a food supplement and traditional herbal medicine for the treatment of liver diseases and alcoholic poisoning for more than a millennium. The objectives of the present study, therefore, were to determine the antioxidant activity of polysaccharides from the peduncles of H. dulcis (HDPS) and to evaluate its hepatoprotective effect on acute alcohol-induced liver injury in mice. HDPS, prepared by hot water extraction, ethanol precipitation and treatment of macroporous resin, was found to be non-starch polysaccharide and mainly composed of galactose, arabinose, rhamnose and galacturonic acid. In in vitro antioxidant assay, HDPS exhibited high superoxide radical scavenging activity, strong inhibition effect on lipid peroxidation and a medium ferrous ion-chelating activity. For hepatoprotective activity in vivo, the administration of HDPS significantly decreased the serum levels of alanine aminotransferase and aspartate aminotransferase, significantly decreased the liver level of malondialdehyde and remarkably restored the liver activities of superoxide dismutase and glutathione peroxidase in alcohol-induced liver injury mice. The results suggested that HDPS had a significant protective effect against acute alcohol-induced liver injury possibly via its antioxidant activity to protect biological systems against the oxidative stress.
    Food and chemical toxicology: an international journal published for the British Industrial Biological Research Association 06/2012; 50(9):2964-70. · 2.99 Impact Factor
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    ABSTRACT: Response surface methodology was employed to optimize the medium compositions for the production of mycelial polysaccharide from Pholiota dinghuensis Bi (PDP). As a result, the optimal medium for crude PDP production was determined as follows (g/L): glucose 36.0, corn flour 11.8, peptone 3.0, yeast extract 5.4, KH(2)PO(4) 1.0, and MgSO(4) 1.5. In a verification experiment, a yield of 756 ± 38 mg/L crude PDP was obtained. Furthermore, biochemical assay and histopathological analysis showed that crude PDP exerted significant hepatoprotective effect in a dose-dependent manner against carbon tetrachloride (CCl(4))-induced acute liver injury. Crude PDP prevented the increase of activities of serum alanine aminotransferase and aspartate aminotransferase, reduced the formation of malondialdehyde and enhanced the activities of superoxide dismutase and glutathione peroxidase in CCl(4)-induced hepatotoxicity in mice.
    Food and chemical toxicology: an international journal published for the British Industrial Biological Research Association 05/2012; 50(8):2681-8. · 2.99 Impact Factor
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    ABSTRACT: Optimization of extraction, preliminary characterization and hepatoprotective activity of polysaccharides from Stachys flordana Schuttl. ex Benth. (SFPS) were investigated in the present study. Firstly, the optimal conditions for extraction of SFPS were obtained by a Box-Behnken design as follows: extraction temperature 94°C, extracting time 4h and ratio of water to material 19mg/ml. Then, the analysis of monosaccharide composition by gas chromatography revealed that SFPS was composed of rhamnose, arabinose, glucose and galactose in a molar percent of 2.05, 9.16, 28.66 and 60.14, respectively. Finally, we demonstrated that SFPS had a significant protective effect against acute hepatotoxicity induced by carbon tetrachloride (CCl4) in mice, as evident by lower levels of serum alanine aminotransferase, aspartate aminotransferase and hepatic malondialdehyde, higher activities of superoxide dismutase and catalase, as well as higher reduced glutathione concentration compared to the CCl4-treated group. The results suggested that SFPS could be explored as novel natural supplement.
    Carbohydrate Polymers - CARBOHYD POLYM. 01/2012;
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    ABSTRACT: SummaryA four‐factor and three‐level Box–Behnken design was used to optimise the extraction parameters for polysaccharides from Allium macrostemon Bunge (AMBP). As a result, the optimal conditions for AMBP extraction were the following: extraction temperature, 87 °C; ratio of water to raw material, 12 mL g−1; extraction time, 100 min; and extraction times, 3. Then, a graded ethanol precipitation was used to fractionate the water‐extractable polysaccharides, resulting in three polysaccharides fractions of AMBP40, AMBP60 and AMBP80. During in vitro antioxidant assay, AMBP40 exhibited relative stronger scavenging activities on hydroxyl radical and superoxide radical and metal chelating activity than AMBP60 and AMBP80. The differences in antioxidant activities in vitro might be due to their differences in molecular weight and uronic acid content.
    International Journal of Food Science & Technology 01/2012; 47(4). · 1.24 Impact Factor
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    ABSTRACT: In the present study, we investigated the extraction, purification, preliminary characterization, antioxidant and anticancer activities in vitro of polysaccharides from Cyclina sinensis (CSPS). Firstly, the optimal parameters for extraction of CSPS were obtained by using a central composite design as follows: extraction temperature, 90 °C; extraction time, 250 min; ratio of water to raw material, 29; and extraction times, two times. Then, the crude CSPS was sequentially purified by chromatography of DEAE-52 and Sephadex G-100, resulting in three purified fractions of CSPS-1, CSPS-2 and CSPS-3. We found that the composition and property of CSPS-3, having relatively higher contents of protein, uronic acid, sulfate and more complicated monosaccharide composition, were quite different from those of CSPS-1 and CSPS-2. Furthermore, we demonstrated that CSPS-3 had strong scavenging activities in vitro on superoxide radical and hydroxyl radical and strong inhibitory effect in vitro on the growth of human gastric cancer BGC-823 cells.
    Carbohydrate Polymers 01/2011; 84(3):851-857. · 3.48 Impact Factor

Publication Stats

34 Citations
17.02 Total Impact Points

Institutions

  • 2011–2013
    • Nanjing Agricultural University
      • College of Food Science and Technology
      Nanjing, Jiangsu Sheng, China