Pedro Luiz Rosalen

University of Campinas, Conceição de Campinas, São Paulo, Brazil

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Publications (122)196.52 Total impact

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    ABSTRACT: Abstract Nearly 20 million tons of winery by-products, with many biological activities, are discarded each year in the world. The extraction of bioactive compounds from Chenin Blanc, Petit Verdot, and Syrah grape by-products, produced in the semi-arid region in Brazil, was optimized by a Central Composite Rotatable Design. The phenolic compounds profile, antioxidant capacity against synthetic free radicals (DPPH and ABTS), reactive oxygen species (ROS; peroxyl radical, superoxide radical, hypochlorous acid), cytotoxicity assay (MTT) and quantification of TNF-α production in RAW 264.7 cells were conducted. Gallic acid, syringic acid, procyanidins B1 and B2, catechin, epicatechin, epicatechin gallate, quercetin 3-β-d-glucoside, delfinidin 3-glucoside, peonidin 3-O-glucoside, and malvidin 3-glucoside were the main phenolic compounds identified. In general, rachis showed higher antioxidant capacity than pomace extract, especially for Chenin Blanc. All extracts showed low cytotoxicity against RAW 264.7 cells and Petit Verdot pomace suppressed TNF-α liberation in vitro. Therefore, these winery by-products can be considered good sources of bioactive compounds, with great potential for application in the food and pharmaceutical industries.
    Food Chemistry 08/2015; DOI:10.1016/j.foodchem.2015.02.087 · 3.26 Impact Factor
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    ABSTRACT: A collection of tagged deletion mutant strains was created in Streptococcus mutans UA159 to facilitate investigation of the aciduric capability of this oral pathogen. Gene-specific barcoded deletions were attempted in 1432 open reading frames (representing 73% of the genome), and resulted in the isolation of 1112 strains (56% coverage) carrying deletions in distinct non-essential genes. Since S. mutans virulence is predicated upon the ability of the organism to survive an acidic pH environment, form biofilms on tooth surfaces, and out-compete other oral microflora, we assayed individual mutant strains for the relative fitness of the deletion strain, compared to the parent strain, under acidic and oxidative stress conditions, as well as for their ability to form biofilms in glucose- or sucrose-containing medium. Our studies revealed a total of 51 deletion strains with defects in both aciduricity and biofilm formation. We have also identified 49 strains whose gene deletion confers sensitivity to oxidative damage and deficiencies in biofilm formation. We demonstrate the ability to examine competitive fitness of mutant organisms using the barcode tags incorporated into each deletion strain to examine the representation of a particular strain in a population. Co-cultures of deletion strains were grown either in vitro in a chemostat to steady-state values of pH 7 and 5 or in vivo in an animal model for oral infection. Taken together, this data represents a mechanism for assessing the virulence capacity of this pathogenic microorganism and a resource for identifying future targets for drug intervention to promote healthy oral microflora. This article is protected by copyright. All rights reserved. This article is protected by copyright. All rights reserved.
    05/2015; DOI:10.1111/omi.12107
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    ABSTRACT: In oral biofilms, two of the major environmental challenges encountered by the dental pathogen Streptococcus mutans are acid and oxidative stresses. Previously, we showed that the S. mutans transcriptional regulators SpxA1 and SpxA2 (formerly SpxA and SpxB, respectively) are involved in stress survival by activating the expression of classic oxidative stress genes such as dpr, nox, sodA and tpx. We reasoned that some of the uncharacterized genes under SpxA1/A2 control are potentially involved in oxidative stress management. Therefore, the goal of this study was to use Spx-regulated genes as a tool to identify novel oxidative stress genes in S. mutans. Quantitative real-time PCR was used to evaluate the responses of ten Spx-regulated genes during H2O2 stress in the parent and Δspx strains. Transcription activation of the H2O2-induced genes (8 out of 10) was strongly dependent on SpxA1 and, to a lesser extent, SpxA2. In vitro transcription assays revealed that one or both Spx proteins directly regulate three of these genes. The gene encoding the FeoB ferrous permease was slightly repressed by H2O2 but constitutively induced in strains lacking SpxA1. Nine genes were selected for downstream mutational analysis but inactivation of smu127, encoding a subunit of the acetoin dehydrogenase was apparently lethal. In vitro and in vivo characterization of the viable mutants indicated that, in addition to the transcriptional activation of reducing and antioxidant pathways, Spx performs an important role in iron homeostasis by regulating the intracellular availability of free iron. In particular, inactivation of the genes encoding the Fe-S biogenesis SUF system and the previously characterized iron-binding protein Dpr resulted in impaired growth under different oxidative stress conditions, increased sensitivity to iron and lower infectivity in rats. These results serve as an entryway into the characterization of novel genes and pathways that allow S. mutans to cope with oxidative stress.
    PLoS ONE 04/2015; 10(4):e0124969. DOI:10.1371/journal.pone.0124969 · 3.53 Impact Factor
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    ABSTRACT: Dental caries remains the most prevalent and costly oral infectious disease worldwide. Several methods have been employed to prevent this biofilm-dependent disease, including the use of essential oils (EOs). In this systematic review, we discuss the antibacterial activity of EOs and their isolated constituents in view of a potential applicability in novel dental formulations. Seven databases were systematically searched for clinical trials, in situ, in vivo and in vitro studies addressing the topic published up to date. Most of the knowledge in the literature is based on in vitro studies assessing the effects of EOs on caries-related streptococci (mainly Streptococcus mutans) and lactobacilli, and on a limited number of clinical trials. The most promising species with antibacterial potential against cariogenic bacteria are: Achillea ligustica, Baccharis dracunculifolia, Croton cajucara, Cryptomeria japonica, Coriandrum sativum, Eugenia caryophyllata, Lippia sidoides, Ocimum americanum, and Rosmarinus officinalis. In some cases, the major phytochemical compounds determine the biological properties of EOs. Menthol and eugenol were considered outstanding compounds demonstrating an antibacterial potential. Only L. sidoides mouthwash (1%) has shown clinical antimicrobial effects against oral pathogens thus far. This review suggests avenues for further non-clinical and clinical studies with the most promising EOs and their isolated constituents bioprospected worldwide.
    Molecules 04/2015; 20(4):7329-7358. DOI:10.3390/molecules20047329 · 2.42 Impact Factor
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    ABSTRACT: The essential oils (EO) and bioactive fractions (BF) from Aloysia gratissima, Baccharis dracunculifolia, Coriandrum sativum, Cyperus articulatus, and Lippia sidoides were proven to have strong antimicrobial activity on planktonic microorganisms; however, little is known about their effects on the morphology or viability of oral biofilms. Previously, we determined the EO/fractions with the best antimicrobial activity against Streptococcus mutans and Candida spp. In this report, we used a confocal analysis to investigate the effect of these EO and BF on the morphology of S. mutans biofilms (thickness, biovolume, and architecture) and on the metabolic viability of C. albicans biofilms. The analysis of intact treated S. mutans biofilms showed no statistical difference for thickness in all groups compared to the control. However, a significant reduction in the biovolume of extracellular polysaccharides and bacteria was observed for A. gratissima and L. sidoides groups, indicating that these BF disrupt biofilm integrity and may have created porosity in the biofilm. This phenomenon could potentially result in a weakened structure and affect biofilm dynamics. Finally, C. sativum EO drastically affected C. albicans viability when compared to the control. These results highlight the promising antimicrobial activity of these plant species and support future translational research on the treatment of dental caries and oral candidiasis.
    Evidence-based Complementary and Alternative Medicine 03/2015; 2015(2015):871316. DOI:10.1155/2015/871316 · 1.88 Impact Factor
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    ABSTRACT: The aim of this study was to evaluate the gastroprotective activity of ethanolic extract of geopropolis (EEGP) from Melipona scutellaris and to investigate the possible mechanisms of action. The gastroprotective activity of the EEGP was evaluated using model ulcer induced by ethanol. To elucidate the possible mechanisms of action, we investigated the involvement of the nonprotein sulfhydryl (NP-SH) groups, nitric oxide and prostaglandins. In addition, the antisecretory activity of EEGP was also evaluated by pylorus ligated model. The EEGP orally administrated (300 mg/kg) reduced the ulcerative lesions induced by the ethanol (). Regarding the mechanism of action, the prior administration of nitric oxide and prostaglandins antagonists suppressed the activity of gastroprotective EEGP (). On the other hand the gastroprotective activity of EEGP was kept in the group pretreated with the antagonist of the NP-SH groups; furthermore the antisecretory activity was not significant (). These results support the alternative medicine use of geopropolis as gastroprotective and the activities observed show to be related to nitric oxide and prostaglandins production.
    Evidence-based Complementary and Alternative Medicine 01/2015; 2015:1-5. DOI:10.1155/2015/459846 · 1.88 Impact Factor
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    ABSTRACT: Objective: to elucidate the mechanisms involved in the anti-inflammatory properties of Brazilian red propolis Method: different concentrations of ethanolic extract of Brazilian red propolis (EEBRP) were added to RAW 264.7 macrophages after activation with LPS, and NO production and cell viability determined. Production of TNF-α, IL1β, TGF-β, IL-4, IL-6, IL-10, IL-12, GM-CSF, IFN-Ɣ and expression of genes related to cytokines production and nitric oxide , PI3K-AKT and signal transduction pathway were evaluated by ELISA and RT-qPCR, respectively. Differences were determined by one-way ANOVA followed by Tukey-Kramer Result: EEBRP inhibited NO production by 69% without affecting cell viability at the concentration of 50 µg/ml when compared with control cells (treated with vehicle) (p<0.05). Levels of IL-12, GM-CSF, IFN-Ɣ, IL-1β, IL-4, IL-10 and TGF-β in cell supernatant were reduced and of TNF- α and IL-6 increased with the addition of EEBRP (p<0.05). EEBRP induced alteration in expression of 57 genes. Genes involved in Toll-like response; oxide nitric sintetase production (all types), Nf-KB and MAPK signaling pathways such as Tirap, Pdk1, Pak1, Nfkb1, Mtcp1, Gsk3b, Fos, Elk1, Il1β, Il1f9 were negatively regulated by EEBRP(fold-change rate > 5; p<0.05) Conclusion: EEBRP presents antiinflammatory properties by altering signaling pathways leading to reduction in production of pro-inflammatory factors.
    2014 IADR/PER Congress; 09/2014
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    ABSTRACT: Objective: recent data have shown that Brazilian red propolis presents immunemodulatory properties, which led us to elucidate the mechanisms involved in the anti-inflammatory properties of one of its components, Neovestitol Method: neovestitol was obtained from ethanolic extract of Brazilian red propolis after bio-guided fractionation coupled with GC-MS analysis. Different concentrations of Neovestitol were added to RAW 264.7 macrophages after activation with LPS. After 48 hours, NO production and cell viability were determined. Production of TNF-α, IL1β, TGF-β, IL-4, IL-6, IL-10, IL-12, GM-CSF, IFN-Ɣ and expression of genes related to cytokines production, nitric oxide , PI3K-AKT and signal transduction pathways were evaluated by ELISA and RT-qPCR, respectively. Differences were determined by one-way ANOVA followed by Tukey-Kramer Result: Neovestitol inhibited NO production by 63% without affecting cell viability at the concentration of 60 µg/ml when compared with control cells (treated with vehicle) (p<0.05). Levels of GM-CSF, IFN- Ɣ, IL-1 β, IL-4, TNF- α and IL-6 in cell supernatant were reduced and of IL-10 increased with the addition of neovestitol (p<0.05). Neovestitol induced alteration in the expression of 43 genes. Genes involved in Toll-like response; nitric oxide sintetase production (all types), Nf-KB and Il-1 signaling pathways such as Bad, Chuk, Elk1, Ifnb1, Il1b, Lif, Calm1, Capns1, Egr1, Nox1, Scd2 and Scd3 were negatively regulated by neovestitol (fold-change rate > 5; p<0.05). Furthermore, its role in inhibition of leukocyte transmigration was suggested by inhibition of expression of Nox1 and in the regulation of fatty acid associated genes such as Scd2 e Scd3 Conclusion: Neovestitol may represent a new antiinflammatory agent by inhibiting signaling pathways and consequently reducing the production of pro-inflammatory factors and by interfering in leukocyte transmigration
    2014 IADR/PER Congress; 09/2014
  • Planta Medica 07/2014; 80(10). DOI:10.1055/s-0034-1382566 · 2.34 Impact Factor
  • Planta Medica 07/2014; 80(10). DOI:10.1055/s-0034-1382571 · 2.34 Impact Factor
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    ABSTRACT: The aim of the present study was to perform an in vitro analysis of the antimicrobial and antiproliferative potential of an extract from Anadenanthera colubrina (Vell.) Brenan (angico) and chemically characterize the crude extract. Antimicrobial action was evaluated based on the minimum inhibitory concentration (MIC), minimum bactericidal/fungicidal concentration, and the inhibition of formation to oral biofilm. Cell morphology was determined through scanning electron microscopy (SEM). Six strains of tumor cells were used for the determination of antiproliferative potential. The extract demonstrated strong antifungal activity against Candida albicans ATCC 18804 (MIC = 0.031 mg/mL), with similar activity found regarding the ethyl acetate fraction. The extract and active fraction also demonstrated the capacity to inhibit the formation of Candida albicans to oral biofilm after 48 hours, with median values equal to or greater than the control group, but the difference did not achieve statistical significance (P > 0.05). SEM revealed alterations in the cell morphology of the yeast. Regarding antiproliferative activity, the extract demonstrated cytostatic potential in all strains tested. The present findings suggest strong antifungal potential for Anadenanthera colubrina (Vell.) Brenan as well as a tendency toward diminishing the growth of human tumor cells.
    Evidence-based Complementary and Alternative Medicine 06/2014; 2014:802696. DOI:10.1155/2014/802696 · 1.88 Impact Factor
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    ABSTRACT: Oral candidiasis is an opportunistic fungal infection of the oral cavity with increasingly worldwide prevalence and incidence rates. Novel specifically-targeted strategies to manage this ailment have been proposed using essential oils (EO) known to have antifungal properties. In this study, we aim to investigate the antifungal activity and mode of action of the EO from Coriandrum sativum L. (coriander) leaves on Candida spp. In addition, we detected the molecular targets affected in whole-genome expression in human cells. The EO phytochemical profile indicates monoterpenes and sesquiterpenes as major components, which are likely to negatively impact the viability of yeast cells. There seems to be a synergistic activity of the EO chemical compounds as their isolation into fractions led to a decreased antimicrobial effect. C. sativum EO may bind to membrane ergosterol, increasing ionic permeability and causing membrane damage leading to cell death, but it does not act on cell wall biosynthesis-related pathways. This mode of action is illustrated by photomicrographs showing disruption in biofilm integrity caused by the EO at varied concentrations. The EO also inhibited Candida biofilm adherence to a polystyrene substrate at low concentrations, and decreased the proteolytic activity of Candida albicans at minimum inhibitory concentration. Finally, the EO and its selected active fraction had low cytotoxicity on human cells, with putative mechanisms affecting gene expression in pathways involving chemokines and MAP-kinase (proliferation/apoptosis), as well as adhesion proteins. These findings highlight the potential antifungal activity of the EO from C. sativum leaves and suggest avenues for future translational toxicological research.
    PLoS ONE 06/2014; 9(6):e99086. DOI:10.1371/journal.pone.0099086 · 3.53 Impact Factor
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    ABSTRACT: The aim of this study was to evaluate the anti-inflammatory activity of Petit Verdot Extract and hexane, chloroform and ethyl acetate fractions obtained from grape pomace, in addition to identifying active compounds. The PVE and EAF reduced significantly paw edema and neutrophil migration when compared with control groups. The PVE reduced levels of TNF-α and IL1-β in the peritoneal fluid, whereas the EAF did not reduce cytokines significantly. Two hydroxybenzoic acids, two proanthocyanidins, three flavan-3-ol monomers and three anthocyanins were identified in the PVE and EAF by LC-MS/MS. The stilbene trans-resveratrol was identified only in the EAF. The phenolic compounds were quantified using HPLC-DAD analysis, except for the stilbenes, which were not sensible for the detection by the method. Since PVE and EAF showed significantly anti-inflammatory effects and high concentration of phenolic compounds, we concluded that Petit Verdot pomace could be an interesting source of anti-inflammatory bioactives.
    Journal of Functional Foods 05/2014; 8:292–300. DOI:10.1016/j.jff.2014.03.016 · 4.48 Impact Factor
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    ABSTRACT: Purpose: To compare the pharmacokinetic profiles and to evaluate the bioequivalence of two commercial amoxicillin suspension formulations (500 mg/5 mL AMOXIL®, reference formulation and AMOXI-PED®, test formulation) in healthy Brazilian volunteers. Methods: Under fasting condition, 25 volunteers (13 males and 12 females) were included in this randomized, open-label, two-period crossover (1-week washout interval) bioequivalence study. Blood samples were collected at pre-dose (0 hour) and 0.5, 1, 1.33, 1.66, 2, 2.5, 3, 4, 6, 8, and 12 hours after drug ingestion. Pharmacokinetic parameters (Cmax, tmax, t1/2, AUC0-tlast, and AUC0-∞) were calculated from plasma concentrations for both formulations in each subject. Results: Arithmetic mean values of the pharmacokinetic parameters were: Cmax = 12.004 (± 2.824) μg×mL-1; tmax = 1.118 (± 0.396) h; t1/2 = 1.226 (± 0.179) h; AUC0-tlast = 29.297 (± 6.007) μg×h×mL-1; and AUC0-∞ = 29.299 (± 6.007) μg×h×mL-1 for reference formulation and Cmax = 11.456 (± 2.825) μg×mL-1; tmax = 1.331 (± 0.509) h; t1/2 = 1.141 (± 0.133) h; AUC0-tlast = 28.672 (± 5.778) μg×h×mL-1; and AUC0-∞ = 28.693 (± 5.796) μg×h×mL-1 for test formulation. The confidence intervals (90% CI) for reference and test formulations were, respectively, 90.74 - 100.46% for Cmax and 93.62 - 103.61% for AUC0-t. Conclusion: Based on the results, both formulations of amoxicillin evaluated in this study were considered bioequivalent according to FDA and ANVISA/Brazil criteria.
    International journal of clinical pharmacology and therapeutics 04/2014; 52(05). DOI:10.5414/CP202039 · 1.04 Impact Factor
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    ABSTRACT: The present study examined the influences of the neovestitol-vestitol (NV) containing fraction isolated from Brazilian red propolis on the development of biofilm and expression of virulence factors by Streptococcus mutans using saliva-coated surfaces of hydroxyapatite. In addition, NV was tested in a rodent model of dental caries to assess its potential effectiveness in vivo. Topical applications of NV (800 μg ml(-1)) significantly impaired the accumulation of biofilms of S. mutans by largely disrupting the synthesis of glucosyltransferase-derived exopolysaccharides and the expression of genes associated with the adaptive stress response, such as copYAZ and sloA. Of even greater impact, NV was as effective as fluoride (positive control) in reducing the development of carious lesions in vivo. NV is a promising natural anti-biofilm agent that targets essential virulence traits in S. mutans, which are associated with the formation of cariogenic biofilm and the subsequent onset of dental caries disease.
    Biofouling 10/2013; DOI:10.1080/08927014.2013.834050 · 3.70 Impact Factor
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    ABSTRACT: Guava pomace is an example of the processing waste generated after the manufacturing process from the juice industry that could be a source of bioactives. Thus, the present investigation was carried out in order to evaluate the anti-inflammatory and antinociceptive potential and determinate the main phenolic compounds of a guava pomace extract (GPE). The anti-inflammatory activity was evaluated by carrageenan, dextran, serotonin, histamine-induced paw edema and neutrophils migration in the peritoneal cavity models. Acetic acid-induced abdominal writhing and formalin test were performed to investigate the antinociceptive effects. In addition, the content of total phenolic and of individual phenolic compounds was determined by GC/MS. GPE showed anti-inflammatory activity by carrageenan, dextran, serotonin, histamine-induced paw edema and neutrophils migration in the peritoneal cavity models (p < 0.05). GPE also demonstrated antinociceptive activity by acetic acid-induced abdominal writhing and formalin test (p < 0.05). The total phenolic value was 3.40 +/- 0.09 mg GAE/g and epicatechin, quercetin, myricetin, isovanilic and gallic acids were identified by GC/MS analysis. The presence of bioactive phenolic compounds as well as important effects demonstrated in animal models suggest that guava pomace could be an interesting source of anti-inflammatory and analgesic substances.
    BMC Complementary and Alternative Medicine 09/2013; 13(1):235. DOI:10.1186/1472-6882-13-235 · 1.88 Impact Factor
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    ABSTRACT: The aim of this study was to evaluate the influence of the bioactive nonpolar fraction of geopropolis on Streptococcus mutans biofilm. The ethanolic extract of Melipona scutellaris geopropolis was subjected to a liquid-liquid partition, thus obtaining the bioactive hexane fraction (HF) possessing antimicrobial activity. The effects of HF on S. mutans UA159 biofilms generated on saliva-coated hydroxyapatite discs were analyzed by inhibition of formation, killing assay, and glycolytic pH-drop assays. Furthermore, biofilms treated with vehicle control and HF were analyzed by scanning electron microscopy (SEM). HF at 250 μ g/mL and 400 μ g/mL caused 38% and 53% reduction in the biomass of biofilm, respectively, when compared to vehicle control (P < 0.05) subsequently observed at SEM images, and this reduction was noticed in the amounts of extracellular alkali-soluble glucans, intracellular iodophilic polysaccharides, and proteins. In addition, the S. mutans viability (killing assay) and acid production by glycolytic pH drop were not affected (P > 0.05). In conclusion, the bioactive HF of geopropolis was promising to control the S. mutans biofilm formation, without affecting the microbial population but interfering with its structure by reducing the biochemical content of biofilm matrix.
    Evidence-based Complementary and Alternative Medicine 06/2013; 2013:256287. DOI:10.1155/2013/256287 · 1.88 Impact Factor
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    ABSTRACT: The aim of this study was to evaluate the activity of the ethanolic extract of geopropolis (EEGP) from Melipona scutellaris and its fractions on the modulation of neutrophil migration in the inflammatory process, and the participation of nitric oxide (NO) pathway, as well as to check the chemical profile of the bioactive fraction. EEGP and its aqueous fraction decreased neutrophil migration in the peritoneal cavity and also the interaction of leukocytes (rolling and adhesion) with endothelial cells. The levels of chemokines CXCL1/KC and CXCL2/MIP-2 were not altered after treatment with EEGP and the aqueous fraction. It was found that the injection of NO pathway antagonists abolished the EEGP and the aqueous fraction inhibitory activity on the neutrophil migration. The expression of intercellular adhesion molecule type 1 (ICAM-1) was reduced, and nitrite levels increased after treatment with EEGP and aqueous fraction. In the carrageenan-induced paw edema model, EEGP and the aqueous fraction showed antiedema activity. No pattern of flavonoid and phenolic acid commonly found in propolis samples of Apis mellifera could be detected in the aqueous fraction samples. These data indicate that the aqueous fraction found has promising bioactive substances with anti-inflammatory activity.
    Evidence-based Complementary and Alternative Medicine 04/2013; 2013:907041. DOI:10.1155/2013/907041 · 1.88 Impact Factor
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    ABSTRACT: The objective of this study was to evaluate anti-inflammatory and antimicrobial activities of neovestitol and vestitol isolated from Brazilian red propolis (BRP). BRP ethanolic extract (EEP), neovestitol and vestitol were evaluated by anti-inflammatory properties using a neutrophil migration assays. The antimicrobial activity was evaluated by minimal inhibitory and bactericidal concentrations (MIC and MBC) against Sreptococcus mutans, Sreptococcus sobrinus, Sthaphylococcus aureus, and Actinomyces naeslundii. Neovestitol, vestitol and EEP inhibited neutrophil migration at dose of 10 mg/Kg. Regarding antimicrobial activity, neovestitol showed MIC ranging from <6.25 to 25-50 µg/mL and MBC ranging from 25-50 to 50-100 µg/mL, while vestitol showed MIC ranging from 25-50 to 50-100 µg/mL and MBC ranging from 25-50 to 50-100 µg/mL. Both isoflavonoids, neovestitol and vestitol, are consistent bioactive compounds displaying anti-inflammatory and antimicrobial activities that can strongly act in low dose and concentration and have a promising potential to be applied on pharmaceutical and food industries.
    Journal of Agricultural and Food Chemistry 04/2013; 61(19). DOI:10.1021/jf305468f · 3.11 Impact Factor
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    ABSTRACT: Objective: The purpose of this study was to evaluate in vitro and in vivo anti-inflammatory properties (in vitro and in vivo) of Brazilian red propolis. Method: Ethanolic extract of Brazilian red propolis (BRP) was investigated on modulation of NO production by macrophages RAW 264.7 (in vitro) and on cells viability. In vivo neutrophil migration inhibition activity induced by carrageenan was also evaluated, as well its mechanism of action such as NO pathway (by previous administration of aminoguanidine, an inhibitor of nitric oxide synthesis), TNF-α, IL-1β and IL-10 (by ELISA). The statistical analysis was performed according to one-way ANOVA followed by Tukey-Kramer for in vitro assays and ANOVA followed by Bonferroni test for in vivo assays. Result: BRP modulated NO production by macrophages at concentrations as low as 60µg/mL without affecting cells viability. BRP was also effective in preventing neutrophil migration to peritoneal cavity at a dose of 10 mg/kg (p<0.05). The in vivo inhibitory effect caused by BRP appears to be associated with inhibition of nitric oxide pathway and with decrease in production of TNF-α without affecting IL-1β (p<0.05) and IL-10 levels. Conclusion: The Brazilian red propolis may be a promising anti-inflammatory natural product, affecting nitric oxide production and cytokines profile.
    IADR/AADR/CADR General Session and Exhibition 2013; 03/2013

Publication Stats

2k Citations
196.52 Total Impact Points

Institutions

  • 1998–2015
    • University of Campinas
      • Faculty of Dentistry from Piracicaba
      Conceição de Campinas, São Paulo, Brazil
  • 1996–2015
    • University of Rochester
      • • Center for Oral Biology
      • • Department of Biomedical Engineering (School of Engineering)
      • • Rochester Center for Economic Research
      Rochester, New York, United States
  • 2009
    • University Center Rochester
      • Center for Oral Biology
      Rochester, Minnesota, United States
  • 2008
    • Faculdade de Ciências da Saúde de São Paulo
      San Paulo, São Paulo, Brazil
    • Universidade Federal de Alfenas
      Alfenas, Minas Gerais, Brazil
  • 2007
    • State University of Ponta Grossa
      • Department of Pharmaceutical Sciences
      Ponta Grossa, Paraná, Brazil