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Publications (2)3.15 Total impact

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    ABSTRACT: Abstract We explored the molecular mechanisms of obesity and insulin resistance in patients with polycystic ovary syndrome (PCOS) using a human embryonic stem cell model (hESCs). Three PCOS-derived and one non-PCOS-derived hESC lines were induced into adipocytes, and then total RNA was extracted. The differentially expressed PCOS-derived and non-PCOS-derived adipocytes genes were identified using the Boao Biological human V 2.0 whole genome oligonucleotide microarray. Signals of interest were then validated by real-time PCR. A total of 153 differential genes were expressed of which 91 genes were up-regulated and 62 down-regulated. Nuclear receptor subfamily 0, group B, member 2 (NR0B2) was an up-regulated gene, and the GeneChip CapitalBio® Molecule Annotation System V4.0 indicated that it was associated with obesity and diabetes (Ratio ≥2.0X). Multiple genes are involved in PCOS. Nuclear receptor subfamily 0, group B, member 2 may play a role in obesity and insulin resistance in patients with PCOS.
    Systems biology in reproductive medicine 02/2014; · 1.85 Impact Factor
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    ABSTRACT: Background: Polycystic ovary syndrome (PCOS) is related to genetic factors. Adipose tissue and insulin resistance (IR) may play an important role in the pathogenesis and progression of PCOS. We investigate glucose consumption and insulin response abilities in the adipocytes differentiated from PCOS-derived human embryonic stem cells (hESCs) in vitro in order to provide a new idea for exploring PCOS pathogenesis. Methods: hESC lines were established with the discarded embryos of non-PCOS or PCOS-women, and then were differentiated into adipocytes. The glucose consumption ability and insulin response ability for these adipocytes were detected. Results: There was no significant difference in glucose consumption ability between non-PCOS and PCOS-derived adipocytes at the absence of insulin. Insulin could significantly increase glucose consumption abilities of both non-PCOS and PCOS-derived adipocytes, but there was no significant difference in the increased glucose consumption ability between the two types of adipocytes. Conclusion: The glucose consumption ability and insulin response ability in the PCOS-derived adipocytes are similar to that in non-PCOS-derived adipocytes.
    Gynecological Endocrinology 05/2012; 28(11):871-3. · 1.30 Impact Factor