Tomohiro Kozako,
Noriko Matsumoto,
Yukako Kuramoto,
Akira Sakata, Rie Motonagare,
Akiyoshi Aikawa,
Masumi Imoto,
Akihisa Toda,
Shin-ichiro Honda,
Hiroshi Shimeno,
Shinji Soeda
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ABSTRACT: Vasohibin is thought to be an important negative feedback regulator of angiogenesis that is selectively induced in endothelial cells by VEGF. Here, we assessed the role of vasohibin on HIF-1α expression under oxidative stress induced by hydrogen peroxide (H₂O₂) in HUVEC. VEGF induced significant cell growth that was associated with an increase in vasohibin expression. Following H₂O₂-pretreatment, VEGF further increased cell growth but this was contrastingly associated with a decrease in vasohibin expression when compared with VEGF alone. Interestingly, vasohibin inhibited cell proliferation through degradation of HIF-1α expression during H₂O₂-pretreatment. Furthermore, vasohibin elevated the expression of prolyl hydroxylase (PHD). These results suggest that vasohibin plays crucial roles as a negative feedback regulator of angiogenesis through HIF-1α degradation via PHD.
FEBS letters 04/2012; 586(7):1067-72. · 3.54 Impact Factor
