Xiang Wang

Shanghai Jiao Tong University, Shanghai, Shanghai Shi, China

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Publications (6)32.16 Total impact

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    ABSTRACT: Embryonic stem cells (ESCs) may be useful as a therapeutic source of cells for the production of healthy tissue; however, they are associated with certain challenges including immunorejection as well as ethical issues. Induced pluripotent stem cells (iPSCs) are a promising substitute since a patient's own adult cells would serve as tissue precursors. Ethical concerns prevent a full evaluation of the developmental potency of human ESCs and iPSCs, therefore, mouse iPSC models are required for protocol development and safety assessments. We used a modified culturing protocol to differentiate pluripotent cells from a mouse iPS cell line and two mouse ES cell lines into neurons. Our results indicated that all three pluripotent stem cell lines underwent nearly the same differentiation process when induced to form neurons in vitro. Genomic expression microarray profiling and single-cell RT-qPCR were used to analyze the neural lineage differentiation process, and more than one thousand differentially expressed genes involved in multiple molecular processes relevant to neural development were identified.
    International Journal of Molecular Medicine 05/2013; · 1.96 Impact Factor
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    ABSTRACT: Human induced pluripotent stem (iPS) cells have the ability to differentiate into all somatic cells and to maintain unlimited self-renewal. Therefore, they have great potential in both basic research and clinical therapy for many diseases. To identify potentially universal mechanisms of human somatic cell reprogramming, we studied gene expression changes in three types of cells undergoing reprogramming. The set of 570 genes commonly regulated during induction of iPS cells includes known embryonic stem (ES) cell markers and pluripotency related genes. We also identified novel genes and biological categories which may be related to somatic cell reprogramming. For example, some of the down-regulated genes are predicted targets of the pluripotency microRNA cluster miR302/367, and the proteins from these putative target genes interact with the stem cell pluripotency factor POU5F1 according to our network analysis. Our results identified candidate gene sets to guide research on the mechanisms operating during somatic cell reprogramming.
    Journal of Genetics and Genomics 12/2012; 39(12):613-23. · 2.08 Impact Factor
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    ABSTRACT: Protein-DNA interactions are involved in many essential biological processes such as transcription, splicing, replication and DNA repair. It is of great value to identify DNA-binding proteins as well as their binding sites in order to study the mechanisms of these biological processes. A number of experimental methods have been developed for the identification of DNA-binding proteins, such as DNAase footprinting, EMSA, X-ray crystallography, NMR spectroscopy and CHIP-on-Chip. However, with the increasingly greater number of suspected protein-DNA interactions, identification by experimental methods is expensive, labor-intensive and time-consuming. Hence, in the past decades researchers have developed many computational approaches to predict in silico the interactions of proteins and DNA. Machine learning technology has been widely used and become dominant in this field. In this article, we focus on reviewing recent machine learningbased progresses in DNA-binding protein and binding residue prediction methods, the most commonly used features in these predictions, machine learning classifier comparison and selection, evaluation method comparison, and existing problems and future directions for the field.
    Protein and Peptide Letters 05/2012; · 1.99 Impact Factor
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    Cell Research 05/2012; 22(6):1082-5. · 10.53 Impact Factor
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    ABSTRACT: Previous studies demonstrated that induced pluripotent stem (iPS) cells could produce viable mice through tetraploid complementation, which was thought to be the most stringent test for pluripotency. However, these highly pluripotent iPS cells were previously reported to be generated from fibroblasts of embryonic origin. Achieving fully pluripotent iPS cells from multiple cell types, especially easily accessible adult tissues, will lead to a much greater clinical impact. We successfully generated high-pluripotency iPS cells from adult tail tip fibroblasts (TTF) that resulted in viable, full-term, fertile TTF-iPS animals with no obvious teratoma formation or other developmental abnormalities. Comparison of iPS cells from embryonic origin (MEF), progenitor cells (neural stem cells) or differentiated somatic cells (TTF) reveals that fully pluripotent developmental potential can be reached by each cell type, although with different induction efficiencies. This work provides the means for studying the mechanisms and regulation of direct reprogramming, and has encouraging implications for future clinical applications and therapeutic interventions.
    Stem cell reviews 09/2010; 6(3):390-7. · 5.08 Impact Factor
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    Cell Research 02/2010; 20(3):383-6. · 10.53 Impact Factor