Chuanliang Guo

Shanghai Jiao Tong University, Shanghai, Shanghai Shi, China

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Publications (2)14.35 Total impact

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    ABSTRACT: Nanog is a well-known transcription factor that plays fundamental roles in stem cell self-renewal and maintaining pluripotent cell identity. There remains a large data gap with respect to the spectrum of the key pluripotency transcription factors' interaction partners. Limited information is available concerning Nanog-associated RNA binding proteins (RBPs), and the intrinsic protein-RNA interactions characteristic of the regulatory activities of Nanog. Herein we used an improved affinity protocol to purify Nanog-interacting RBPs from mouse embryonic stem cells (ESCs), and 49 RBPs of Nanog were identified. Among them, the interaction of YBX1 and ILF3 with Nanog mRNA were further confirmed by in vitro assays such as Western Blot, RNA immunoprecipitation (RIP), and ex vivo methods such as Immunofluorescence staining and Fluorescent In Situ Hybridization (FISH), MS2 in vivo Biotin Tagged RNA Affinity Purification (MS2-BioTRAP). Interestingly, RNAi studies revealed that YBX1 and ILF3 positively affected the expression of Nanog and other pluripotency-related genes. Particularly, down-regulation of YBX1 or ILF3 resulted in high expression of mesoderm markers. Thus, a reduction in the expression of YBX1 and ILF3 controls the expression of pluripotency-related genes in ESCs, suggesting their roles in further regulation of the pluripotent state of ESCs. This article is protected by copyright. All rights reserved.
    Cell Biology International 08/2015; DOI:10.1002/cbin.10539 · 1.93 Impact Factor
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    ABSTRACT: Cell death and differentiation is a monthly research journal focused on the exciting field of programmed cell death and apoptosis. It provides a single accessible source of information for both scientists and clinicians, keeping them up-to-date with advances in the field. It encompasses programmed cell death, cell death induced by toxic agents, differentiation and the interrelation of these with cell proliferation.
    Cell Research 05/2012; 22(6):1082-5. DOI:10.1038/cr.2012.77 · 12.41 Impact Factor

Publication Stats

11 Citations
14.35 Total Impact Points


  • 2012-2015
    • Shanghai Jiao Tong University
      • Institute of Medical Genetics
      Shanghai, Shanghai Shi, China