C C Chien,
P Y Tseng,
H H Chen, T E Hua,
S T Chen,
Y Y Chen,
W H Leng,
C H Wang,
Y Hwu,
G C Yin,
K S Liang,
F R Chen,
Y S Chu,
H I Yeh,
Y C Yang,
C S Yang,
G L Zhang,
J H Je,
G Margaritondo
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ABSTRACT: Our experimental results demonstrate that full-field hard-X-ray microscopy is finally able to investigate the internal structure of cells in tissues. This result was made possible by three main factors: the use of a coherent (synchrotron) source of X-rays, the exploitation of contrast mechanisms based on the real part of the refractive index and the magnification provided by high-resolution Fresnel zone-plate objectives. We specifically obtained high-quality microradiographs of human and mouse cells with 29nm Rayleigh spatial resolution and verified that tomographic reconstruction could be implemented with a final resolution level suitable for subcellular features. We also demonstrated that a phase retrieval method based on a wave propagation algorithm could yield good subcellular images starting from a series of defocused microradiographs. The concluding discussion compares cellular and subcellular hard-X-ray microradiology with other techniques and evaluates its potential impact on biomedical research.
Biotechnology advances 04/2012; · 8.25 Impact Factor
