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ABSTRACT: A novel Gram-stain-positive actinobacterium, designated HR08-44(T), was isolated from a sea sediment sample collected from the foreshore of Rishiri Island, Japan, and its taxonomic position was investigated by a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequence comparisons revealed that strain HR08-44(T) was closely related to the members of the genus Janibacter with pairwise sequence similarities of 97.3-98.8%. Strain HR08-44(T) had peptidoglycan of A1γ type, with meso-diaminopimelic acid as the diagnostic diamino acid. The predominant menaquinone was MK-8(H4) and the major fatty acids were iso-C16:0, C17:1 ω8c, C17:0 and C18:1 ω9c. These data supported the affiliation of the novel strain to the genus Janibacter. Meanwhile, the results of DNA-DNA hybridization, physiological and biochemical tests indicated that strain HR08-44(T) should be distinguished from the recognized Janibacter species. Therefore, strain HR08-44(T) represents a novel species of the genus Janibacter, for which the name Janibacter cremeus sp. nov. is proposed; the type strain is HR08-44(T) (=NBRC 107693(T) =DSM 26154(T)).
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 04/2013; · 2.11 Impact Factor
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ABSTRACT: A novel Gram-positive actinobacterium, designated H25-14T, was isolated from a sea sediment sample, and its taxonomic position was investigated by a polyphasic approach. The peptidoglycan type of strain H25-14T was A4α and lysine was the diagnostic diamino acid of the peptidoglycan. The predominant menaquinone was MK-9(H4) and the major fatty acids were anteiso-C15:0 and iso-C15:0. The DNA G+C content was 73.2 mol%. Phylogenetic analysis based on 16S rRNA gene sequence comparisons revealed that strain H25-14T was closely related to Paraoerskovia marina NBRC 104352T (98.3 %). However, the result of DNA-DNA hybridization and phenotypic characteristics revealed that strain H25-14T differed from P. marina. Therefore, strain H25-14T represents a novel species of the genus Paraoerskovia, for which the name Paraoerskovia sediminicola sp. nov. is proposed. The type strain is H25-14T (=NBRC 108565T =DSM 25477T).
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 01/2013; · 2.11 Impact Factor
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ABSTRACT: Two novel Gram-positive actinobacteria, designated H97-3(T) and H83-5, were isolated from marine sediment samples and their taxonomic positions were investigated by a polyphasic approach. Both strains formed vegetative hyphae in the early phase of growth but the hyphae eventually fragmented into coccoid cells. The peptidoglycan type was found to be A4α. The predominant menaquinone was MK-9(H(4)), and the major fatty acids were anteiso-C(15:0), anteiso-C(17:0) and C(16:0). The DNA G+C content was 74.0-74.9 mol %. 16S rRNA gene sequencing analysis revealed that strains H97-3(T) and H83-5 represented novel members of the family Cellulomonadaceae. Their nearest phylogenetic neighbours were the members of the genus Oerskovia, with a similarity of 98.3-98.4 %. However, strains H97-3(T) and H83-5 were distinguishable from the members of the genus Oerskovia and the other genera of the family Cellulomonadaceae in terms of chemotaxonomic characteristics and phylogenetic relationship. The result of the DNA-DNA hybridization indicated that strains H97-3(T) and H83-5 belonged to the same species. Therefore, strains H97-3(T) and H83-5 represent a novel genus and species of the family Cellulomonadaceae, for which the name Sediminihabitans luteus gen. nov., sp. nov. is proposed. The type strain of S. lutes is H97-3(T) (=NBRC 108568(T) = DSM 25478(T)).
Antonie van Leeuwenhoek 04/2012; 102(2):325-33. · 2.09 Impact Factor
