Publications (2)5.38 Total impact
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Article: BcNRT1, a plasma membrane-localized nitrate transporter from non-heading Chinese cabbage.
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ABSTRACT: A nitrate transporter, BcNRT1, was isolated from non-heading Chinese cabbage (Brassica campestris ssp. chinensis Makino) cultivar 'Suzhouqing'. The full-length cDNA was obtained using the rapid amplification of cDNA ends technique and contains an open reading frame of 1,770 bp that predicts a protein of 589 acid residues that possesses 12 putative transmembrane domains. Using the GUS marker gene driven by the BcNRT1 promoter, we found BcNRT1 expression to be concentrated in primary and lateral root tips and in shoots of transgenic Arabidopsis plants. The YFP fused to BcNRT1 and transformed into cabbage protoplasts indicated that BcNRT1 was localized to the plasma membrane. The expression of BcNRT1 in roots was induced by exposure to 25 mM nitrate, and the BcNRT1 cRNA heterologously expressed in Xenopus laevis oocytes showed nitrate conductance when nitrate was included in the medium. Moreover, mutant chl1-5 plants harboring 35S::BcNRT1 showed sensitivity to chlorate treatment and exhibited restored nitrate uptake. In conclusion, the results indicate that BcNRT1 functions as a low affinity nitrate transporter in non-heading Chinese cabbage.Molecular Biology Reports 04/2012; 39(8):7997-8006. · 2.93 Impact Factor -
Article: Cloning and Characterization of the BcTuR3 Gene Related to Resistance to Turnip Mosaic Virus (TuMV) from Non-heading Chinese Cabbage
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ABSTRACT: A cDNA clone for the turnip mosaic virus (TuMV)-induced gene in non-heading Chinese cabbage (Brassica campestris ssp. chinensis Makino cv. Duanbaigeng) was isolated and characterized. The full-length cDNA clone, designated BcTuR3, was isolated during resistance response to TuMV. Sequence analysis of the cDNA clone confirmed that the translation product of the gene is homologous to other plant resistance proteins. Genomic DNA Southern blot analysis indicated that the gene represented a small multi-gene family. Northern hybridizations confirmed its elevated expression in the resistant “Duanbaigeng” and the susceptible “Aijiaohuang.” Upon inoculation with TuMV, the BcTuR3 transcript was rapidly accumulated in the infected leaves and stems. Tissue-specific expression of the gene showed that higher levels of the BcTuR3 transcript were observed in the TuMV-infected leaves, and later in the stems and roots of the resistant and susceptible varieties. These data showed that the BcTuR3 gene may be involved in plant resistance against TuMV pathogen infection. KeywordsTurnip mosaic virus (TuMV)-Non-heading Chinese cabbage (Brassica campestris ssp. chinensis Makino)-Antiviral protein-Real-time quantitative PCR-Systemic acquired resistancePlant Molecular Biology Reporter 04/2012; 28(4):588-596. · 2.45 Impact Factor
