Yahong Ding

Publications (2)4.45 Total impact

• Article: Induction of cross-protection against influenza A virus by DNA prime-intranasal protein boost strategy based on nucleoprotein.

  Jian Luo, Dan Zheng, Wenjie Zhang, Fang Fang, Hanzhong Wang, Ying Sun, Yahong Ding, Chengfei Xu, Quanjiao Chen, Hongbo Zhang, Ding Huang, Bing Sun, Ze Chen
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  ABSTRACT: BACKGROUND: The highly conserved nucleoprotein (NP) is an internal protein of influenza virus and is capable of inducing cross-protective immunity against different influenza A viruses, making it a main target of universal influenza vaccine. In current study, we characterized the immune response induced by DNA prime-intranasal protein boost strategy based on NP (A/PR/8/34, H1N1) in mouse model, and evaluated its protection ability against a lethal dose challenge of influenza virus. RESULTS: The intranasal boost with recombinant NP (rNP) protein could effectively enhance the pre-immune response induced by the NP DNA vaccine in mice. Compared to the vaccination with NP DNA or rNP protein alone, the prime-boost strategy increased the level of NP specific serum antibody, enhanced the T cell immune response, and relatively induced more mucosal IgA antibody. The overall immune response induced by this heterologous prime-boost regimen was Th-1-biased. Furthermore, the immune response in mice induced by this strategy provided not only protection against the homologous virus but also cross-protection against a heterosubtypic H9N2 strain. CONCLUSIONS: The NP DNA prime-intranasal protein boost strategy may provide an effective strategy for universal influenza vaccine development.
  Virology Journal 11/2012; 9(1):286. · 2.34 Impact Factor
• Article: Intranasal immunization with live attenuated influenza vaccine plus chitosan as an adjuvant protects mice against homologous and heterologous virus challenge.

  Xueliang Wang, Wenjie Zhang, Fen Liu, Mei Zheng, Dan Zheng, Tao Zhang, Yinglei Yi, Yahong Ding, Jian Luo, Chaoyang Dai, Hanzhong Wang, Bing Sun, Ze Chen
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  ABSTRACT: Our previous studies have proven the adjuvanticity of chitosan in mice when administered with inactivated and subunit influenza vaccine. In this study, we investigated the adjuvant effect of chitosan on the immunogenicity and protective efficacy of a live attenuated influenza vaccine. Mice were inoculated intranasally with live attenuated influenza vaccine plus chitosan and then challenged with a high, lethal dose of homologous or heterologous virus. Antibody responses, secretion of IFN-γ by spleen cells, body weight loss, survival rates, and residual lung virus titers were tested. The results demonstrated that live attenuated influenza vaccine with chitosan adjuvant not only protected mice completely against challenge with the homologous virus but also provided good cross-protection against a heterologous virus. In addition, chitosan as adjuvant could significantly increase the levels of antigen-specific antibodies and the population of IFN-γ-secreting T cells. These results reveal the potential of chitosan as a candidate adjuvant for use in a live attenuated influenza vaccine.
  Archives of Virology 05/2012; 157(8):1451-61. · 2.11 Impact Factor