[show abstract][hide abstract] ABSTRACT: Statins are a commonly prescribed cholesterol-lowering drug; however, it has recently been shown that
they also have the beneficial side effect of enhancing bone matrix formation. As a result, this study is to assay
probability effect of Pravastatin on osteogenesis which is made in experimental flaw and it is as a laboratory pattern
in rat femur. This experimental study was conducted on 30 male SD rats. Animals were divided randomly into 3
groups (control and experimental). After induction of general anesthesia, a hole in size of 2 mm in diameter was
made using a dental bit in femur width to medullary channel. After surgery, the control group received orally sterile
water daily and experimental groups 1 and 2 respectively received daily 10 and 20 mg/kg/PO of Pravastatin.
Histopathological and histomorphometrical studies for evaluation of bone healing were carried out in experimental
rats, which were euthanized after 45 days of the experiment. In control group, defect seemed to be filled with woven
bone and bone marrow spaces and in spite of a poor osteogenic activity. In experiment groups, many osteoblasts
groupings, and young bone trabeculas increased in number and bone trabeculas more organized. Histomorphometric
results, observed that Pravastatin has significant effect on bone healing in experimental groups 2 and 3 than control
group (p=0/000), analyzing obtained results show that Pravastatin has significant effect in group 3 that received high
dosage of Pravastatin than group 2 (p=0/000). The results of this study show that Pravastatin could stimulate
osteogenesis in rats.
[show abstract][hide abstract] ABSTRACT: The aim of the present in vivo study was to determine bone tissue reaction to calcium enriched mixture (CEM) and mineral trioxide aggregate (MTA) using a rat femur model.
Sixty-three rats were selected and randomly divided into three groups of 21 each [experimental groups (n=15), control (n=6)]. Implantation cavities were prepared in each femoral bone and randomly filled with the biomaterials only in the experimental groups. The animals in three groups were sacrificed 1, 4, and 8 weeks postoperatively. Histologic evaluations comprising inflammation severity and new bone formation were blindly made on H&E-stained decalcified 6-µm sections.
At 1, 4, and 8 weeks after implantation number of inflammatory cells had decreased in the CEM, MTA and control groups, respectively, with no statistically significant differences. Conversely, new bone formation had increased in all the experimental and control groups, without statistically significant differences.
The results suggest that biocompatibility of MTA, as gold standard, and CEM cement as a new endodontic biomaterial are comparable.
[show abstract][hide abstract] ABSTRACT: Although diabetic hepatopathy is potentially less common, it may be appropriate for addition to the list of target organ conditions related to diabetes. This study was designed to evaluate the hepatoprotective properties of green tea extract (GTE) in STZ-induced diabetes in rats. Wistar rats were made diabetic through single injection of STZ (75 mg/kg i.p.). The rats were randomly divided into four groups of 10 animals each: Group 1, healthy control; Group 2, nondiabetics treated with GTE administered orally (1.5%, w/v); Group 3, diabetics; Group 4, diabetics treated with GTE (1.5%, w/v) for 8 weeks. Serum biomarkers were assessed to determine hepatic injury. Malondialdehyde (MDA) and reduced glutathione (GSH) contents were measured to assess free radical activity in the liver tissue. Hepatic antioxidant activities of glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), and catalase (CAT) were also determined. The biochemical findings were matched with histopathological verifications. Liver MDA content and serum levels of ALT, AST, ALP, and bilirubin in Group 3 significantly increased compared to Group 1 (P < 0.05) and significantly decreased in Group 4 compared to Group 3 (P < 0.05). Serum albumin level and GSH, SOD, CAT, and GSH-Px contents of the liver in Group 3 were significantly decreased compared to Group 1 (P < 0.05) and were significantly increased in Group 4 compared to Group 3 (P < 0.05). Histopathologically, the changes were in the same direction with biochemical findings. This study proved the hepatoprotective activity of GTE in experimentally induced diabetic rats.
Evidence-based Complementary and Alternative Medicine 01/2012; 2012:740671. · 1.72 Impact Factor
[show abstract][hide abstract] ABSTRACT: Diabetes mellitus is a systemic disease that has profound deleterious effect on bone healing, diabetes is associated with a decrease in skeletal mass and delayed healing of fractures. The treatment of massive bone defects in diabetic patients is the most challenging problem; many researchers are trying to find materials that can improve bone healing in diabetic patients. Bone matrix gelatin (BMG) is recently introduced. The purpose of this study was to evaluate of histopathologically effects of bone matrix gelatin on cancellous bone defect healing in diabetic rat models. The experiment was conducted on 20 male adult SD rats, after inductions of diabetes were divided into two groups of control and experiment. After general anesthesia, a hole in size of 2×3 mm in diameter and depth was made using a dental bit in the inner aspect of the between condyles of left femur. In control group defect was left untreated and in experimental group allogeneic BMG was used to fill the bone defect. The BMG was prepared as previously described using Urist method. After 45 days all rats were euthanized. The samples were stained by H&E and histopathologically evaluation of the distal femoral bone was performed. Histologic studies performed in control group, showed the immature granulation tissue was observed in the remaining portions of the defects. Extension of lamellar bone from the lateral aspects of the defects to the middle portions of that was prominent. In experiment group, significant bone formation could be observed and almost all defects were filled with newly formed bone and
[show abstract][hide abstract] ABSTRACT: Pathogenic role of free radicals are well known in various metabolic diseases. They originate from internal and external sources of body. Essential roles of antioxidant defense system for cellular redox regulation and free radical scavenging activity were described in this study. Many in vitro investigations have shown that turmeric (TE) and carrot seed extract (CSE) exhibits to possess antioxidant activities. In this study, we evaluated the antioxidant potentials of ethanolic TE and CSE based on in vivo experiment in the rats.
ANIMALS WERE ASSIGNED TO SIX GROUPS: the 1st and 2nd groups were control groups and 2nd group received 0.2 ml dimethyl sulphoxide as vehicle treated group; other four experimental groups received different doses of TE (100, 200 mg/kg b.w.) and CSE (200, 400 mg/kg b.w.) by gavages, respectively for a period of one month. The indicators of oxidative stress, lipids peroxidation, markers of hepatocyte injury and biliary function markers were measured.
The levels of superoxide dismutase, catalase, and glutathione peroxidase were significantly stimulated in the hepatic tissue of treatment groups. The malondialdehyde contents of liver tissue were significantly reduced in the groups fed with TE and CSE. Serum levels of alanine aminotransferase, aspartate aminotransferase and alkaline phosphatase, in treated groups were found to be significantly decreased, whereas albumin and total protein increased as compared to the control groups (P<0.05).
this study showed that the regular intake of TE and CSE through the diet can improve antioxidant status and inhibit peroxidation activity in the liver tissue so that using these extracts may protect tissue oxidative stress.
[show abstract][hide abstract] ABSTRACT: Objective/hypothesis A comparison of the histopathological effect of topical use of Mitomycin C and 5-Fluorouracil in preventing myringotomy closure in rats. Study design: clinical trial. Methods and materials The study was performed on 43 rats that were divided into three groups. Study groups (A and B) and control group (C) after bilateral cold-knife myringotomy, we applied Mitomycin C (MMC) 4mg/ml to group A, 5-Fluouracil (5FU) 50mg/ml to group B, and normal saline to group C. An examination of all ears of rats was carried out by otoscope on days 0, 1, 3, 5, 7, and then every five days up to 70 days. Each day's closed myringotomies of all groups were examined. Results The mean of post myringotomy opening time was 37, 16, and 12 days respectively in MMC, 5FU, and saline. Patency duration of MMC group was significantly long (p<0.0001), but in histopatholgical examinations, sclerosis of tympanic membrane in MMC group showed the highest patency duration (p<0.0001). Conclusion Mitomycin C significantly prolonged the duration of myringotomy patency time - longer than 5-Fluouracil and saline but with the adverse effects of tympanic membrane fibrosis.
International journal of pediatric otorhinolaryngology 11/2011; 76(1):9-13. · 0.85 Impact Factor
[show abstract][hide abstract] ABSTRACT: The aim of this study was to determine the effect of PRP on healing of femoral cancellous bone defect in diabetic rabbit. The experiment was conducted on 20 male adult New Zealand white rabbits. After induction of diabetes rabbits were divided into two groups of control and experiment of 10 rabbits each. After general anesthesia, a hole in size of 4×5 mm in diameter and depth was made using a dental bit between lateral and medial condyles of left femor. In control group, defect was left untreated and in experiment group, the created hole was filled with autologous PRP. Five rabbits were sacrificed at 1 and 2 month after surgery and evaluated histopathologically. Histomorphometrically evaluation was compared with healthy bone samples of 2 month after surgery. Data were submitted to statistical analysis by variance analysis (ANOVA) and Tukey test, at a significance level of 5% (p<0.05). In diabetic control group, within 1 month, immature granulation tissue was observed. In 2 month, some more mature lamellar bone along with areas of connective tissue could be observed. In diabetic experiment group, within 1 month, mature granulation tissue formed, within 2 month, bone formation could be observed, and bone neoformation was compact, with bone trabeculas. Differences observed in histomorphometrically findings were significant between diabetic control group and diabetic experiment group (p=0/001). The results of this study show that PRP provide a rapid regeneration of bone defects in femoral cancellous bone in diabetic rabbits.
Australian Journal of Basic and Applied Sciences. 01/2011; 5:800-808.
[show abstract][hide abstract] ABSTRACT: Two methods employed for palmar digital neurectomy are the guillotine and epineural capping techniques. The present investigation was primarily conducted to compare the histhopatologic effects of the techniques said above. Ten Guillotine and the Epineural capping neurectomy were performed bilaterally in ten horses (five horse in each group). The nerve ends were evaluated microscopically after 60 days of surgery. The outcomes of study suggest that the guillotine technique has a lower incidence of painful neuroma formation than epineural capping technique and it also has the advantages of shorter surgery time, less expense and lower incidence of re-innervation. We presume that the guillotine method produced less painful neuromas because, through the guillotine method of neurectomy, the nerve was sharply transected and stretched from the surgically traumatized tissue.
[show abstract][hide abstract] ABSTRACT: Methotrexate (MTX) was originally used as part of combination chemotherapy regimens to treat many kinds of cancers. MTX treatment is often limited by severe side effects; Hepatotoxicity, acute hepatitis, chronic fibrosis/cirrhosis is some of the major toxic effects of MTX for liver. This study was designed to investigate whether carrot seed (Daucus Carota L.) had a protective effect on methotrexate-induced liver damage. Forty-two wistar strain rats were divided into 7 groups (n=6) during 8 days treatment: Group1 control; Group2 dimethyl sulfoxide(DMSO), vehicle drug; Group3 MTX(20mgkg-1); Group4 carrot seed (200 mgkg-1) ; Group5 carrot seed (400mgkg-1); Group6 low dose carrot seed with MTX; Group7 high dose carrot seed with MTX. All animals were sacrificed 4 days after intraperitoneal injection of MTX then liver tissue for histopathological examination and to obtain liver tissue to study glutathione peroxidase(GPx), superoxide dismutase(SOD), malondialdehyde(MDA) levels and aminotransferase(AST) and alanine aminotransferase(ALT) were measured in serum. The result showed that GPx and SOD levels were increase but MDA level in liver were decrease by curcumin treatment and level AST and ALT were tend to increase in serum level but not significant Histopathological examination indicated carrot seed extract can overcome reproductive toxicity of methotrexate and decrease oxidative stress and can be protecting liver tissue. The present study indicates that curcumin may be of therapeutic benefit agains MTX-cytotoxcity.
The 21st Conference of the Asian Pacific Association for the Study of the Liver (APASL 2011) 17-20 February 2011, QSNCC, Bangkok Thailand. 01/2011;
[show abstract][hide abstract] ABSTRACT: I/R-induced acute renal failure (ARF) is a common clinical problem, which despite significant advances in critical care medicine are still associated with high morbidity and mortality.
Advances in Environmental Biology 01/2011; 5:1622-1626.
[show abstract][hide abstract] ABSTRACT: Osteogenesis is one the essential elements of bone regeneration along with the final binding between host bone and grafting material. Collagen type I (Col) is one of the most widely used bone-filling biomaterial in present bone tissue engineering. Calcium phosphate bone cements (CPC) have gained importance in orthopaedics as repair materials for bone defects. A combination of Col/CPC mixture would probably create a composite with both osteoconductive and osteoinductive properties. The purpose of this study was to determine the effect of the combination of collagen type I and calcium phosphate bone cement mixture on healing of rabbit radius segmental defects. 54 adult male New Zealand white rabbits were used. A segmental bone defect of 10 mm in length was created in the middle of the right radius shaft. The defects were stabilized with miniplate in control group and CPC was used to fill the bone defect in group II and a mixture of CPC/Col was used to fill the bone defect in group III. The animal was evaluated clinically and radiologically in the postoperative period. Rabbits were sacrificed at 4, 8 and 12 weeks after surgery and evaluated histopathologically. Differences observed in radiological findings were not significant between groups but differences observed in histopathological findings were significant between group 3 and groups 1 and 2 and also was significant between group 2 and group 1. The results of this study show that the mixture of collagen type I and calcium phosphate bone cement is a good choice for the healing of segmental bone defects, and provides a more rapid regeneration of bone defects.
Australian Journal of Basic and Applied Sciences. 01/2010; 4:5144-5153.
[show abstract][hide abstract] ABSTRACT: Problem statement: The purpose of this study was to histopathology and biomechanics evaluate the effect of sodium fluoride on cancellous bone defect healing in rat model. Approach: The experiment was conducted on 40 male adult SD rats which were divided into four groups of control and experiments. After induction of general anesthesia, a hole in size of 2×3 mm in diameter and depth was made using a dental bit in the inner aspect of the between condyles of right femor. In all groups, defect was left untreated. Control group was given distilled water and the other three groups were exposed to fluoridated water at different concentrations (8, 30 and 60 mg F -/L). After 45 days all rats were sacrificed and the histopathological and biomechanical penetration tests of the distal femoral bone were performed. Results: In control group, defect seemed to be filled with adipous tissue and sparse hematogenic cells and in spite of a poor osteogenic activity and some osteoblasts could already be seen. In experiment groups, many osteoblasts groupings and young bone trabeculas increased in number and bone trabeculas more organized. Histopathologically findings show that 30 mg F -/L sodium fluoride in drinking water has been better effect than other groups. The mean load for fracturing in control group was 173.01±23.05 and in experiment groups 8, 30 and 60mg were 177.31±33.71, 181.90±36.81 and 168.51±54.35. Differences observed in biomechanically findings were significant between 30mg group and other groups (p<0.05). Conclusion: The results of this study show that fluoride level at 30 mg F -L −1 in drinking water increases compressive strength of cancellous bone defect in healing process and could stimulate osteogenesis in femoral cancellous bone defect in rats.
American journal of pharmacology and toxicology 01/2010; 5:177-182.
[show abstract][hide abstract] ABSTRACT: Abstract
Bcl-2 plays an important role in apoptosis regulation of
solid tumors, such as colon and breast cancer. The purpose
of this study is to determine bcl-2 expression on aberrant
crypt foci epithelial following treatment with Calendula
officinalis extract. In this study, 56 male wistar rats with
approximate age of 12 weeks and 200-300g weight were
randomly allocated into two equal groups (treatment &
control). For induction of colorectal carcinoma these two
groups were injected by 1, 2-dimethylhydrazine (40mg/kg)
subcutaneously (2 injection/week for two weeks).
Treatment group simultaneously received orally the
Calendula officinalis extract (200mg/kg/day) for 10 weeks.
After 10 weeks, tissue specimens were collected from
distal parts of colon in both experimental groups and 3-4?
m thick microscopic sections were prepared through
immunohistchemistry staining method.
Immunohistopathologi cal study revealed that expression
of BCL2 protein in aberrant crypt foci of colon in treatment
group in comparison with control group was lesser. Mean
difference between two experimental groups was
significant (p<0.01). This in-vivo study indicated that
BCL2 protein expression decreases in aberrant crypt foci
epithelial cells of experimental colorectal carcinoma in rats
following orally administration of Calendula officinalis
Islamic.Azad.Univ., Garmsar Branch Veterinary Research Bulletin. 01/2009; 5:109- 114.
[show abstract][hide abstract] ABSTRACT: Background and Aim: Nowadays pain sedation and faster healing of surgical wounds is more challenging
to researchers. The aim of the present study was to evaluate histopathological effects of acetaminophen, as a
non-opiod analgesic without anti-inflammatory effects, on healing of stomach surgical wounds in the rats.
Materials and Methods: Fifteen male Wistar rats were randomly assigned to three groups (sham, negative
control and experimental) with five subjects in each. A gastrotomy incision with one cm in length was made
in the greater curvature of stomach in the rats, which was thereafter sutured in 2 layers. The experimental
group was orally given acetaminophen (30 mg/kg) solved in dimethyl sulfoxide (DMSO) 5% (10 mL/Kg)
daily for 15 days. The negative control and sham groups received DMSO 5% (10 mL/Kg) and normal saline
(10 mL/Kg) respectively in the same manner as the experimental group. Histopathological studies and
comparison of wound healing among the groups were carried out considering the more effective factors on
wound healing such as proliferation of fibroblasts, angiogenesis, re-epithelialization and collagen production.
Significant differences among the groups were determined by one-way analysis of variance (ANOVA) using
SPSS statistical software at the significant level of P<0.05.
Results: There were no significant differences among the groups regarding the number of fibroblasts,
capillary buds, epithelial gap size, and collagen content in healing of surgical wounds.
Conclusion: The results indicated that acetaminophen has no adverse side-effects on surgical wound
healing of the stomach in rats; thus, it can be used as a pain relief after stomach surgery.
Journal of Birjand University of Medical Sciences. 01/2009;