Renqiang Li

Publications (3)5.21 Total impact

• Article: [Single-step purification of galectin in earthworm using sepharose CL-6B affinity chromatography].

  Yihong Chen, Huimin Huang, Renqiang Li
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  ABSTRACT: The S-type lectins in annelida are different in molecular structure and biochemical properties from that of common galectin and are valuable in anticancer study. Based on their specificity, Sepharose CL-6B affinity chromatography was adopted in this study to separate the S-type lectin in annelida from earthworms, in which EDTA-MEPBS (2 mmol/L EDTA, 4 mmol/L beta-mercaptoethanol, 150 mmol/L NaCl, 20 mmol/L phosphate, pH 7.2) was used as an equilibrium solution and aqueous ammonia solution (150 mmol/L, pH 10.5) as an eluent. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) showed that the relative molecular mass of the obtained protein in this purification procedure was about 32 000 and it could be further separated into fractions with relative molecular mass about 15 000. Hemagglutination experiment and fluorescence analysis demonstrated that this protein possessed the characteristics of agglutinin and its complex with lactose. This indicated that the target protein was one of the S-type lectins in annelida. This study may offer a novel and rapid method for purifying the S-type lectins in annelida in large scale.
  Se pu = Chinese journal of chromatography / Zhongguo hua xue hui 06/2007; 25(3):332-6.
• Article: Immobilized hemin affinity chromatography as a probe for proteins having potentiality to bind with heme.

  Renqiang Li, Fengyi Jiang, Xiaofen Zhang, Yao Chen, Ling Fang
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  ABSTRACT: After Sepharose 4B polymer beads were activated by using epichlorohydrin, hemin was binded with them to prepare an immobilized hemin affinity chromatography column. The coupling rate of this column was very high, more than 0.25mg hemin could be fixed by 1g of wet Sepharose 4B beads. The column equilibrated with deionized water and eluated with pH 3.0 NaAc-HAc buffer was applied to capture the proteins in human serum, earthworm body and Bacillus subtilis cells. Three polypeptides in human serum were captured, one of which was verified as serum albumin after comparison to the control. At least one polypeptide in earthworm body, two in Bacillus subtilis cells displayed the powerful binding specificity to hemin. Our experiments demonstrated that the immobilized hemin affinity chromatography was available as a probe for some proteins having potentiality to bind with heme.
  Journal of Chromatography B 09/2006; 840(1):63-8. · 2.89 Impact Factor
• Source

  Available from: lancang-mekong.org

  Article: Assessment of water quality and identification of pollution sources of plateau lakes in Yunnan (China).

  Renqiang Li, Ming Dong, Yang Zhao, Lili Zhang, Qingguo Cui, Weiming He
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  ABSTRACT: The plateau lakes of Yunnan are important both ecologically and economically in China. Nevertheless, the human impact on water quality in these lakes has become increasingly highlighted. The water quality of 10 plateau lakes was monitored regularly over the period of 2000 through 2004 for 24 parameters. Multivariate statistical techniques, including cluster analysis (CA), factor analysis (FA), and principal component analysis (PCA), were employed to better interpret information about the water quality and its pollution sources. No obvious data reduction from CA/FA was found because three principal components (PCs) needed 14 variables to explain 85.01% of the total variance. However, three latent factors accounted for pollution mainly from the following sources: agricultural activities, residential activities and anthropogenic-toxic pollution from industrial effluents, or other special activities. Box-whiskers plots were employed to visually interpret the spatiotemporal variations of water quality variables, which were highly correlated with three PCs. Three types of water quality (i.e., low-, medium-, and high-polluted lakes) were determined through CA based on the similarity of water quality variables. Our results may provide helpful information for the authorities to effectively manage the water quality and make sound policies.
  Journal of Environmental Quality 36(1):291-7. · 2.32 Impact Factor