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Publications (3)4.05 Total impact

  • Xinxin Lu, Haitong Gu
    Zhonghua yi xue za zhi. 08/2014; 94(32):2481-4.
  • Lijun Wang, Haitong Gu, Xinxin Lu
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    ABSTRACT: An internally controlled real-time PCR assay based on SYBR Green was developed to screen Klebsiella pneumoniae carbapenemases (KPCs) gene containing bacteria and was validated for clinical strains or surveillance specimens. When 248 clinical samples were tested, the sensitivity and specificity of the assay were 100% and 99%, respectively.
    Journal of microbiological methods 09/2012; · 2.43 Impact Factor
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    Lijun Wang, Haitong Gu, Xinxin Lu
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    ABSTRACT: Klebsiella pneumonia carbapenemases (KPCs) are able to hydrolyze the carbapenems, which cause many bacteria resistance to multiple classes of antibiotics, so the rapid dissemination of KPCs is worrisome. Laboratory identification of KPCs-harboring clinical isolates would be a key to limit the spread of the bacteria. This study would evaluate a rapid low-cost real-time PCR assay to detect KPCs. Real-time PCR assay based on SYBR GreenIwas designed to amplify a 106 bp product of the blaKPC gene from the 159 clinical Gram-negative isolates resistant to several classes of -lactam antibiotics through antimicrobial susceptibility testing. We confirmed the results of real-time PCR assay by the conventional PCR-sequencing. At the same time, KPCs of these clinical isolates were detected by the modified Hodge test (MHT). Then we compared the results of real-time PCR assay with those of MHT from the sensitivity and specificity. Moreover, we evaluated the sensitivity of the real-time PCR assay. The sensitivity and specificity of the results of the real-time PCR assay compared with those of MHT was 29/29(100%) and 130/130(100%), respectively. The results of the real-time PCR and the MHT were strongly consistent (Exact Sig. (2-tailed) =1. 000; McNemar test). The real-time PCR detection limit was about 0.8 cfu using clinical isolates. The real-time PCR assay could rapidly and accurately detect KPCs -harboring strains with high analytical sensitivity and specificity.
    Annals of Clinical Microbiology and Antimicrobials 04/2012; 11:9. · 1.62 Impact Factor