Camille Dubois

Publications (3)12.91 Total impact

• Article: Cleavage and protection of locked nucleic acid-modified DNA by restriction endonucleases.

  Lucile Crouzier, Camille Dubois, Jesper Wengel, Rakesh N Veedu
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  ABSTRACT: Locked nucleic acid (LNA) is one of the most prominent nucleic acid analogues reported so far. We herein for the first time report cleavage by restriction endonuclease of LNA-modified DNA oligonucleotides. The experiments revealed that RsaI is an efficient enzyme capable of recognizing and cleaving LNA-modified DNA oligonucleotides. Furthermore, introduction of LNA nucleotides protects against cleavage by the restriction endonucleases PvuII, PstI, SacI, KpnI and EcoRI.
  Bioorganic & medicinal chemistry letters 06/2012; 22(14):4836-8. · 2.65 Impact Factor
• Article: Stepping towards highly flexible aptamers: enzymatic recognition studies of unlocked nucleic acid nucleotides.

  Camille Dubois, Meghan A Campbell, Stacey L Edwards, Jesper Wengel, Rakesh N Veedu
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  ABSTRACT: Enzymatic recognition of unlocked nucleic acid (UNA) nucleotides was successfully accomplished. Therminator DNA polymerase was found to be an efficient enzyme in primer extension reactions. Polymerase chain reaction (PCR) amplification of a 81 mer UNA-modified DNA library was efficiently achieved by KOD DNA polymerase.
  Chemical Communications 04/2012; 48(44):5503-5. · 6.17 Impact Factor
• Source

  Available from: Rakesh N Veedu

  Article: Efficient reverse transcription using locked nucleic acid nucleotides towards the evolution of nuclease resistant RNA aptamers.

  Lucile Crouzier, Camille Dubois, Stacey L Edwards, Lasse H Lauridsen, Jesper Wengel, Rakesh N Veedu
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  ABSTRACT: Modified nucleotides are increasingly being utilized in the de novo selection of aptamers for enhancing their drug-like character and abolishing the need for time consuming trial-and-error based post-selection modifications. Locked nucleic acid (LNA) is one of the most prominent and successful nucleic acid analogues because of its remarkable properties, and widely explored as building blocks in therapeutic oligonucleotides. Evolution of LNA-modified RNA aptamers requires an efficient reverse transcription method for PCR enrichment of the selected RNA aptamer candidates. Establishing this key step is a pre-requisite for performing LNA-modified RNA aptamer selection. In this study three different reverse transcriptases were investigated towards the enzymatic recognition of LNA nucleotides. Both incorporation as well as reading capabilities of the LNA nucleotides was investigated to fully understand the limitations of the enzymatic recognition. We found that SuperScript® III Reverse Transcriptase is an efficient enzyme for the recognition of LNA nucleotides, making it a prime candidate to be used in de novo selection of LNA containing RNA aptamers.
  PLoS ONE 01/2012; 7(4):e35990. · 4.09 Impact Factor