Yuan-yuan Liu

Shanghai Jiao Tong University, Shanghai, Shanghai Shi, China

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Publications (3)0 Total impact

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    ABSTRACT: The colloidal gold immunochromatographic test strip was developed in order to establish a rapid detection assay of Domoic acid (DA) content in marine shellfish. The colloidal gold with particle diameter 20 nm was obtained by reducing gold chloride with sodium citrate. After identification by electron micrograph, optimum conditions for labeling were determined and colloidal gold was labeled by DA monoclonal antibody. The gold-labeled antibody was coated on some chosen glass fiber and dried. The coating antigen (DA-BSA) and Goat anti Mouse IgG were spotted respectively on a piece of nitrate fiber membrane as test line and control line. Finally the test strips were constructed and the detection sensitivity was measured. The results showed that, the detection limit of colloidal gold immunochromatographic test strip was 20 ng/mL and the whole analysis process could be completed within 15 min. The method established is sensitive and the procedure of determination is simple and quick without special equipment. The colloidal gold immunochromatographic test strip could be widely used for batch detection of domoic acid in shellfish on site and has great prospect for commercial development.
    Huan jing ke xue= Huanjing kexue / [bian ji, Zhongguo ke xue yuan huan jing ke xue wei yuan hui "Huan jing ke xue" bian ji wei yuan hui.] 08/2011; 32(8):2492-6.
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    ABSTRACT: A rapid biochemical method was discussed in order to detect low concentration of paralytic shellfish poisoning (PSP) in sea food. The mice were injected with PSP extract of bivalves (1 and 0.2 microg/kg respectively, as STX equivalents) purchased from seafood market. ACh, AChE, NO and NOS in blood were studied at 15, 60, 120 min respectively. The results showed that at low dose (0.2 microg/kg) and 15 min, only the contents of ACh changed significantly compared with control group (p < 0.05), which was (141.2 +/- 14.8) microg/mg, while the contents of NO and the activities of NOS changed until 120 min, compared to control group (p < 0.05) ,which were (68.7 +/- 3.8) micromol/g and (40.1 +/- 4.9) U/mg respectively. At high dose the contents of ACh changed at all three time point. It can be suggested that the contents of ACh is the only one of four indexes which can response to PSP at low dose in an early stage (15 min) and may be selected as a biochemical index for rapid detection of PSP.
    Huan jing ke xue= Huanjing kexue / [bian ji, Zhongguo ke xue yuan huan jing ke xue wei yuan hui "Huan jing ke xue" bian ji wei yuan hui.] 07/2010; 31(7):1663-6.
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    ABSTRACT: In order to explore the production of hydroxyl radical (·OH) in a confined space, a novel ozone-light irradiation system is constructed in this study, and the ·OH radical is measured by spin-trapping electron spin resonance (ESR) method in which 5,5-dimethyl-1-pyrroline-N-oxide (DMPO) is selected as the spin-trap. Several influence factors including the light intensity, the irradiation time and DMPO mass concentration are discussed. The results show that in this experimental system, with DMPO mass concentration of 1g/L and the irradiation time of 30 min, the ·OH radical can be best captured. Besides, both wavelength and intensity of the irradiation light could effect the generation of ·OH radical. These results are of great importance to further study the sterilization effect of ·OH radical in confined space. Key wordselectron spin resonance (ESR)-hydroxyl radicals-ozone-photolysis CLC numberO 482.53
    Journal of Shanghai Jiaotong University (Science) 01/2010; 15(4):458-462.