[Show abstract][Hide abstract] ABSTRACT: Cutaneous mast cell tumors (MCTs) are among the most frequent malignant tumors in dogs and Boxer breed dogs have a higher incidence of this disease. Ki67 and KIT staining are widely used to predict natural behavior in canine MCT but no previous study has evaluated double staining of these proteins as a prognostic factor. Based on biological behavior predictors in canine MCT, the purpose of this study was to determine the Ki67 proliferative index in KIT positive cells using double stain immunohistochemistry technique. Sixty-nine MCTs from Boxer dogs were selected and a tissue microarray was constructed for the double stained immunohistochemistry. Double positivity (Ki67+/ KIT+) was observed in 20/69 (29%) MCT, with a mean of 9.06 double positive cells per tissue core (range 0.48% - 43.97%) and Ki67-/KIT+ animals had a longer survival time than Ki67+/KIT+ animals (p=0.03).
Research in Veterinary Science 08/2015; DOI:10.1016/j.rvsc.2015.08.007 · 1.41 Impact Factor
[Show abstract][Hide abstract] ABSTRACT: A ten-year-old male Burmese python (Python molurus bivitattus) was evaluated for coelomic distension in the second third of the body in the area around the heart. On radiographic examination an increase in soft tissue opacity was seen superimposed over the cardiac silhouette. Ultrasonographic examination revealed a cystic mass in the heart. The snake died after the examination. Necropsy revealed severe distension in cardiac region with coelomic membrane adhesion to the pericardium, which was thickened and filled with a massive blood clot. The heart was increased in size with a mass inside the right atrium. Histological presentation was consistent with septic cardiovascular thrombus with signs of recanalization, diffuse necrosis and acute diffuse inflammatory reaction. Gram staining technique revealed the presence of Gram negative bacteria. The exact cause of the thrombus and the entry site for bacteria could not be determined, but chronic stomatitis that was present for more than seven years in this patient was suggested as a possible origin. Nevertheless, there are only a few reports of cardiac diseases in snakes and generally with scant information concerning the aetiology. In this work, besides reporting details of the case itself possible causes for this cardiac disease are discussed.
[Show abstract][Hide abstract] ABSTRACT: A 12-year-old male English Pointer was examined due to a soft-tissue swelling at the medial canthus of the right orbital region, which was causing facial deformity. The dog had epiphora, purulent nasal discharge, epistaxis, dyspnea, and progressive weight loss. An intraoral mass was observed near the right maxillary premolars. Neoplastic disease was diagnosed based on ancillary tests, which included blood work, skull and intraoral radiographs, ocular ultrasonography and computed tomography. Histopathology revealed transitional carcinoma involving the nasal and oral cavities, maxilla, bony orbit and retrobulbar space. Nasal tumors represent approximately 2% of all tumors diagnosed in this species. Transitional carcinoma is the second most common type of malignant epithelial tumor in the nasal sinuses. This case illustrates the extensive destruction of the soft and bony tissues of the face, including the bony orbit that this type of tumor can cause.
Arquivo Brasileiro de Medicina Veterinária e Zootecnia 08/2013; 65(4):1017-1023. DOI:10.1590/S0102-09352013000400012 · 0.24 Impact Factor
[Show abstract][Hide abstract] ABSTRACT: A female, eight-year-old, mixed-breed blue-eyed dog was presented for ophthalmic evaluation because its left eye had "changed color" one year previously. The before left eye was enucleated and submitted for evaluation. Histopathological analysis revealed an invasive neoplastic mass effacing most of the ventral aspect of the iris stroma. A diagnosis of an anterior uveal spindle cell tumor was made. Immunohistochemical results were strongly suggestive of a schwannoma, but some smooth muscle differentiation was also observed. Two and a half years after therapeutic enucleation there was no evidence of neoplasm recurrence or metastasis.
[Show abstract][Hide abstract] ABSTRACT: A 7-year-old, male mixed-breed dog was presented for evaluation of a well-delineated, pink, oval and slightly firm mass with a smooth regular surface that was expanding approximately 60% of the lower eyelid conjunctiva and the lateral canthus. The dog had a supposed primary B-cell lymphoma at the temporal canthus of the upper eyelid conjunctiva of the same eye that had been removed approximately 3 years earlier. No metastases were detected at either presentation. Histologically, the conjunctival lamina propria was effaced by a well-delineated, unencapsulated and expansile highly cellular neoplasm composed of sheets of round cells that were immunohistochemically positive for CD20 and CD79a negative for CD3. Based on the microscopic and immunohistochemical findings, a diagnosis of supposed primary B cell conjunctival lymphoma was made. Primary ocular and adnexal ocular lymphomas in dogs are rarely reported and their behavior is poorly characterized. Further tumor recurrence was not observed one year post operatively. This case was considered unusual because of its conjunctival involvement and the clinical course with recurrence after three years of a surgical therapeutic procedure.