Amee Patel

Anna University, Chennai, Chennai, Tamil Nādu, India

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Publications (2)4.02 Total impact

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    ABSTRACT: Urinary tract infection (UTI) is among the most common bacterial infections and poses a significant healthcare burden. Escherichia coli is the most common cause of UTI accounting for up to 70 % and a variable contribution from Proteus mirabilis, Pseudomonas aeruginosa and Klebsiella pneumoniae. To establish a complete diagnostic system, we have developed a single-tube multiplex PCR assay (mPCR) for the detection of the above-mentioned four major uropathogens. The sensitivity of the assay was found to be as low as 10(2) cfu/ml of cells. The mPCR evaluated on 280 clinical isolates detected 100 % of E. coli, P. aeruginosa, P. mirabilis and 95 % of K. pneumonia. The assay was performed on 50 urine samples and found to be specific and sensitive for clinical diagnosis. In addition, the mPCR was also validated on spiked urine samples using 40 clinical isolates to demonstrate its application under different strain used in this assay. In total, mPCR reported here is a rapid and simple screening tool that can compete with conventional biochemical-based screening assays that may require 2-3 days for detection.
    Current Microbiology 04/2012; 65(1):44-53. DOI:10.1007/s00284-012-0126-3 · 1.42 Impact Factor
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    ABSTRACT: Diamond-like nanocomposite (DLN) thin films are deposited on glass (pyrex/silicon) substrate by plasma enhanced chemical vapor deposition (PECVD) technique using different combinations of hexamethyldisiloxane (HMDSO) and hexamethyldisilazane (HMDSN) gas precursors. The surface morphology of the DLN films has been investigated by atomic force microscopy (AFM). Nanoparticles on DLN films were analyzed by high-resolution transmission electron microscopy (HRTEM). DLN films contain Si3N4, SiC and SiO x nanoparticles (sizes ∼ 6 –30 nm) within amorphous matrix which reduce the compressive stress of the films and increase the adhesiveness between the films and substrates. The biocompatibility of the films is verified using cell adhesion and morphology studies of the DLN films. We have also observed the fluorescence emission from the nanoparticles of the films. The biocompatibility of DLN films is examined by the cell viability test. This article emphasizes on the possible biomedical applications of prepared films like biosensors for diagnostics and therapies, surgical instruments, prosthetic replacements etc. Chemically modified DLN surface can be act as a sensing trace of gases to detect biomolecules in biological research.
    Science of Advanced Materials 01/2012; 4(1):114-120. DOI:10.1166/sam.2012.1259 · 2.60 Impact Factor