W.P. Chou

National Taiwan University, Taipei, Taipei, Taiwan

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Publications (5)3.96 Total impact

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    L S Kuo, W P Chou, P H Chen
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    ABSTRACT: This work studied the thermal convection under various slip boundary conditions in a 2D box with aspect ratio equal to two. The slip parameter is the normalized tangential momentum accommodation coefficient (TMAC, 0 <= sigma <= 1). The results show that the slip boundary conditions of vertical side walls (a,) and horizontal plates (o-h) will affect the pattern selections of the flow and temperature fields. When sigma(h) < 0.02, the pattern is the one-roll mode for all sigma(v). When sigma(h) <= 0.02 and sigma(v) <= 0.1, the fluids prefer the two-roll mode where two rolls make the fluids to move upwards in the middle of the box. While sigma(h) >= 0.02 and sigma(v) >= 0.2, the fluids prefer the other two-roll mode which makes the fluid to move downwards in the middle of the box. (C) 2010 Elsevier Ltd. All rights reserved.
    International Journal of Heat and Mass Transfer 01/2011; 54(7-8):1340-1343. · 2.52 Impact Factor
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    ABSTRACT: Different from traditional detection methods which were expensive and time consuming for biochemical analyses, a DNA chip assay by using gold nanoparticles (AuNPs) immunological amplification methods and radio frequency techniques was applied to detect DNA sequences rapidly and conveniently. First the capture DNA (cDNA) was immobilized to the substrate on which the LC shunt located. Then the target DNA (tDNA) and probe DNA (pDNA) with AuNP at the 3' end were added to form AuNP-linking structures. Furthermore, the S11 and S21 curves of the biosensors changed due to the AuNP-linking structures between the interdigital and meander waveguides. The changes in S11 and S21 determined whether the tDNA existed. Due to the dual-mode design of the DNA sensors, this platform has a potential for multi-sample detection. The study provided an alternatively simple, fast and convenient solution for biochemical detection.
    Nano/Micro Engineered and Molecular Systems, 2007. NEMS '07. 2nd IEEE International Conference on; 02/2007
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    ABSTRACT: Different from traditional detection methods including polymerase chain reaction (PCR) based assays and enzyme-linked immunosorbent assays (ELISA) which are expensive and time consuming for biochemical analysis, in our study, a DNA chip assay using gold nanoparticle (AuNP) immunological amplification method and RFID communication platform were applied to detect DNA sequences rapidly and conveniently. First the capture DNA (cDNA) was immobilized to the substrate on which the capacitor locates. Then the target DNA (tDNA) and probe DNA (pDNA) with a AuNP at the 3' end were added to form several layers of AuNP linked structures. Since the permittivity of the medium in which the capacitor is located changed due to the AuNP-linking structures between the gaps of the microcapacitor, the resonant frequency of series LC circuit shifted immediately. The lab-on-a-chip proposed in this study was power supplied by the radiofrequency identification (RFID) system. The shift of the LC resonant frequency determined whether the tDNA existed. Thus the detection chip needed no any other internal power supply or expensive instruments for electrical signal measurement. The study provides a simple, fast and convenient platform for biochemical detection
    Nano/Micro Engineered and Molecular Systems, 2006. NEMS '06. 1st IEEE International Conference on; 02/2006
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    ABSTRACT: The current real-time PCR (polymerase chain reaction) platforms, which can detect and quantify several target DNA simultaneously, are equipped with discrete optics and detectors for different fluorescence wavelengths. However, the optical loss, due to the different lengths of the channels for several dyes, lowers the performance of fluorescence detection. Especially on the PCR platforms of lab-on-a-chip system, for the dispersion of the fluorescence in the micro fluidic channels, the received fluorescence is much lower than the emitted. To enhance the received intensity on the detection system is therefore a critical issue. The proposed fluorescence detection system, composing of an ultra-sensitive spectrometer, can provide continuous wavelength detection and can be employed for multiple DNA quantification and genotyping in a single reaction. For the tests to the genotyping ability, the melting temperatures of B type HBV and C type HBV can be distinguished by the difference of 1.1degC.The test results in this research show the same degree of sensitivity for DNA quantification and reproducibility within five intra assay samples as compared with a commercial one
    Nano/Micro Engineered and Molecular Systems, 2006. NEMS '06. 1st IEEE International Conference on; 02/2006
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    ABSTRACT: Due to the increasing demand of simultaneous DNA detection, known as multiplexing detection, in a single sample, the current real-time polymerase chain reaction (PCR) platforms are equipped with discrete optics and detectors for different fluorescence wavelength. However, to go beyond four DNA labeling dyes, the optical loss will lower the performance on fluorescence detection. The proposed fluorescence detection system, composing of an ultra-sensitive spectrometer, can provide continuous wavelength detection and can be employed for multiple DNA quantification. The results show that this prototype provides comparable sensitivity and amplification efficiency as Roche Light-Cycler for DNA quantification and similar reproducibility within five intra assay samples with the DNA quantification method proposed in this study. (c) 2006 Elsevier B.V. All rights reserved.
    Optics Communications 01/2006; 266(2):744-750. · 1.44 Impact Factor

Publication Stats

2 Citations
3.96 Total Impact Points

Institutions

  • 2006–2011
    • National Taiwan University
      • Department of Mechanical Engineering
      Taipei, Taipei, Taiwan
    • National Taiwan Normal University
      • Department of Mechatronic Technology
      T’ai-pei, Taipei, Taiwan