J Y Jung

Chonnam National University, Gwangju, Gwangju, South Korea

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Publications (65)103.58 Total impact

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    ABSTRACT: SETTING: A novel effective treatment is necessary for severe asthma.OBJECTIVE: To review clinical trials examining the role of tiotropium in patients with poorly controlled asthma despite inhaled corticosteroid use with or without long-acting β2-agonists.DESIGN: A computerised search of electronic databases (Medline, EMBASE and Cochrane Central Register) was performed. Randomised controlled trials of at least a 4-week treatment duration with findings published in English were included.RESULTS: Five studies involving 1635 patients were analysed. Compared with a placebo or a double dose of inhaled corticosteroids, the addition of tiotropium increased mean trough and peak forced expiratory volume in 1 second by 97 ml (95%CI 71–122) and 103 ml (95%CI 42–163), respectively. The mean differences in morning peak expiratory flow were 19.2 l/min (95%CI 11.8–26.6). Tiotropium also reduced the risk of severe acute exacerbation (OR 0.73, 95%CI 0.56–0.96) and improved Asthma Quality-of-Life Questionnaire score significantly by 0.10 (95%CI 0.04–0.16). There were no differences in serious adverse events.CONCLUSION: The addition of tiotropium may be beneficial for patients with poorly controlled asthma, although exacerbation or safety issues should be clarified in long-term trials before its wide use in asthma.
    The International Journal of Tuberculosis and Lung Disease 12/2014; 18(12). DOI:10.5588/ijtld.14.0275 · 2.76 Impact Factor
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    ABSTRACT: Osteoprotegerin (OPG) is secreted by stromal and osteoblastic lineage cells and inhibits osteoclastogenesis by preventing the interaction of receptor activator of nuclear factor-κB ligand (RANKL) with receptor activator of nuclear factor-κB (RANK). In this study, the expression of OPG in osteoclasts themselves and its biological functions during osteoclastogenesis were investigated for the first time. OPG expression in vivo in the developing rat maxilla was examined by immunofluorescence analysis. OPG expression in osteoclasts during in vitro osteoclastogenesis was determined by reverse-transcription polymerase chain-reaction (RT-PCR), Western blot, and immunofluorescence staining. We determined the function of OPG produced by osteoclasts during osteoclastogenesis by silencing the OPG gene. The effects of OPG on bone-resorbing activity and apoptosis of mature osteoclasts were examined by the assay of resorptive pit formation on calcium-phosphate-coated plate and terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) staining, respectively. In the immunofluorescence findings, strong immunoreactivities were unexpectedly seen in multinucleated tartrate-resistant acid phosphatase (TRAP)-positive osteoclasts around the growing and erupting tooth germs in the rat alveolar bone. In vitro, OPG expression was significantly increased during the differentiation of osteoclasts from mouse bone-marrow-derived cells treated with a combination of macrophage colony-stimulating factor (M-CSF) and RANKL. Interestingly, it was found that OPG small interfering (si)RNA treatment during osteoclastogenesis enhanced the sizes of osteoclasts, but attenuated their bone-resorbing activity. Also, the increased chromosomal DNA fragmentation and caspase-3 activity in the late phase of osteoclastogenesis were found to be decreased by treatment with OPG siRNA. Furthermore, effects of OPG siRNA treatment on osteoclastogenesis and bone-resorbing activity were recovered by the treatment of exogenous OPG. These results suggest that OPG, expressed by the osteoclasts themselves, may play an auto-regulatory role in the late phase of osteoclastogenesis through the induction of apoptosis.
    Journal of Dental Research 09/2014; 93(11). DOI:10.1177/0022034514552677 · 4.14 Impact Factor
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    ABSTRACT: To investigate immunity-related guanosine triphosphatase family M (IRGM) genetic variants associated with susceptibility to tuberculosis (TB) in a Korean population. We conducted a prospective case-control study including 193 patients with active TB in Severance Hospital and 230 age- and sex-matched unrelated controls registered in Yonsei Cardiovascular Genome Center. Based on associations with other chronic inflammatory conditions, we analyzed the allele and genotype frequencies of rs72553867, rs10065172, and rs12654043 among patients with TB and healthy controls. The T allele of rs10065172 was significantly associated with protection against developing TB based on allele frequency [P = 0.042; odds ratio (OR) 0.75] and genotype distribution in the codominant model (P = 0.036; OR 0.73). This is the first study to identify a significant association between the IRGM single-nucleotide polymorphism (SNP) rs10065172 and susceptibility to active TB disease in an Asian population. The results suggest that IRGM genetic variants could be associated with susceptibility to active TB disease in the Korean population.
    Infection 03/2014; 42(4). DOI:10.1007/s15010-014-0604-6 · 2.86 Impact Factor
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    ABSTRACT: To investigate the relationship between body mass index (BMI), fat free mass index (FFMI) and obstructive lung disease in Korea. Based on a large population-based, nationwide survey conducted in Korea, 822 subjects with airway obstruction and the same number of healthy control subjects were selected. Spirometry and dual-energy X-ray absorptiometry were used for analysis. Subjects with airway obstruction had a lower mean BMI and FFMI than the control group (23.6 vs. 23.9 kg/m(2) for BMI, P = 0.015 and 17.2 vs. 17.5 kg/m(2) for FFMI, P = 0.013); the BMI (P < 0.001) and FFMI (P < 0.001) values decreased significantly in subjects with severe airway obstruction. The proportion of subjects who were underweight or who had a low FFMI was significantly higher in the severe airway obstruction group (P < 0.001). The decrease in FFMI was more prominent in the lower extremities, followed by the upper extremities and the trunk. Subjects who were underweight had significantly lower one-second forced expiratory volume (FEV1; P = 0.001) and FEV1/forced vital capacity values (P < 0.001). We suggest that lower BMI and FFMI are associated with degree of airway obstruction and that the assessment of BMI and body composition is necessary in patients with severe airway obstruction.
    The International Journal of Tuberculosis and Lung Disease 01/2014; 18(1):102-8. DOI:10.5588/ijtld.13.0212 · 2.76 Impact Factor
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    ABSTRACT: Several in vitro studies have been conducted regarding the immunomodulatory and mycobactericidal roles of vitamin D in tuberculous infection. However, discrepancies exist among epidemiological studies. We compared vitamin D deficiency between patients with tuberculosis (TB) and healthy control subjects and identified risk factors for vitamin D deficiency. This was an age- and sex-matched case-control analysis of 94 TB cohort and 282 Korean national survey participants. The median baseline 25-hydroxyvitamin D (25[OH]D) level in the TB group (9.86 ng/ml, IQR 7.19-14.15) was lower than in controls (16.03 ng/ml, IQR 12.38-20.30, P < 0.001). The prevalence of severe vitamin D deficiency was higher in patients with TB (51.1%) than in controls (8.2%, P = 0.001). The median 25(OH)D level increased from 11.40 ng/ml (IQR 7.85-15.73) to 13.18 ng/ml (IQR 10.60-19.71) after treatment completion (P = 0.037). On multivariate analysis, presence of TB and history of TB were independently associated with severe vitamin D deficiency. Patients with TB had a higher prevalence of vitamin D deficiency than control subjects in a Korean population. The median 25(OH)D level increased after TB treatment. Further studies are needed to establish a causal relationship.
    The International Journal of Tuberculosis and Lung Disease 01/2014; 18(1):73-8. DOI:10.5588/ijtld.13.0536 · 2.76 Impact Factor
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    ABSTRACT: Odontogenesis, tooth development, is derived from two tissue components: ectoderm and neural crest-derived mesenchyme. Cyto-differentiation of odontogenic cells during development involves time-dependent and sequential regulation of genetic programs. This study was conducted to detect molecules implicated in cyto-differentiation of developing molar germs of rats. Differential display-PCR revealed that PrP(c) was differentially expressed between cap/early bell-staged germs (maxillary 3rd molar germs) and root formation-staged germs (maxillary 2nd molar germs) at postnatal day 9. Both levels of PrP(c) mRNA and protein expression were higher in the root formation stage than the cap/early bell stage and increased in a time-dependent manner. Immunofluorescence revealed for the first time that PrP(c) was not localized in the enamel organ, but localized in dental follicular cells for the development of the periodontal ligament and cementum as well as odontoblasts, both of which are of neural crest origin. These results suggest that the physiological functions of the PrP(c) in tooth development may be implicated in the differentiation of neural crest-derived mesenchyme including the periodontal tissues for root formation rather than epithelial tissue. Anat Rec, 2013. © 2013 Wiley Periodicals, Inc.
    The Anatomical Record Advances in Integrative Anatomy and Evolutionary Biology 12/2013; 296(12). DOI:10.1002/ar.22807 · 1.53 Impact Factor
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    ABSTRACT: Health care-associated pneumonia (HCAP) affects a heterogeneous group of patients in frequent contact with health care systems. However, HCAP criteria poorly predict infection with drug-resistant (DR) pathogens. To validate our previously reported risk-scoring model (predictive of DR pathogen infection) in patients admitted to hospital with pneumonia. We evaluated 580 patients admitted with culture-positive bacterial pneumonia. We identified risk factors, evaluated the risk-scoring model's capacity to predict infection by DR pathogens and compared the model's diagnostic accuracy with that of current HCAP criteria. DR pathogens were observed in 227/580 patients (39.1%). Of 269 HCAP patients, 153 (56.9%) were infected with DR pathogens. Overtreatment was more common in HCAP than in community-acquired pneumonia (58.7% vs. 41.2%, P < 0.001). Recent hospitalisation, admission from a long-term care facility, recent antibiotic treatment and tube feeding were independently associated with DR pathogens. For pathogen prediction, the risk-scoring model showed better diagnostic accuracy than HCAP criteria (area under receiver operating-characteristic curve = 0.723 vs. 0.673, P < 0.001). According to current HCAP criteria, half of the HCAP patients were treated unnecessarily with broad-spectrum antibiotics. Risk scoring by stratifying risk factors could improve the identification of patients likely to be infected with DR pathogens.
    The International Journal of Tuberculosis and Lung Disease 05/2013; 17(5):704-9. DOI:10.5588/ijtld.12.0723 · 2.76 Impact Factor
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    ABSTRACT: Objectives: Biodentine has been commerciallized and advertized as a bioactive material. The powder is mainly composed of tricalcium silicate, calcium carbonate and zirconium oxide. The liquid contains water, calcium chloride and modified polycarboxylate. The main benefits of Biodentine is the reduced setting time and better mechanical properties. Bioaggregate is also recently developed as a bioceramic material. It is composed of tricalcium silicate, dicalcium silicate, tantalum pentoxide, and calcium phosphate. However, the biologic effect of these new developed cement was not clearly investigated. The purpose of this study was to compare the mineralization effect of these cement with MTA and reveal the possible pathway of mineralizaion. Methods: Human dental pulp cells were isolated from third molars and maintained in the a-MEM, 10% FBS in 5% CO2at 37°C. Dental pulp cells were exposed to the extract of MTA, Biodentine, and Bioaggregate. Cell viability was measured using a MTT assay. RT-PCR was performed with a mineralization specific markers in order to evaluate the mineralization effect. For mineralization nodule assay, alizarin red S staining was performed. The effect on MAPK pathways was assessed by western blot examination of the production of ERK, p38, and JNK. Results: RT-PCR results showed that DSPP and DMP1 expression were increased in the extract of MTA, Biodentine, and Bioaggregate. Alizarin red staining was increased in the extract of MTA, Biodentine, and Bioaggregate. MAPK pathway was affected by extract of MTA, Biodentine, and Bioaggregate Conclusions: These results suggest that Biodentine and Bioaggregate have a mineralization effect on human dental pulp cells.
    IADR/AADR/CADR General Session and Exhibition 2013; 03/2013
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    ABSTRACT: Aims:  To establish an efficient genetic transformation protocol for Leuconostoc species, methods for competent-cell preparation and electroporation conditions were optimized. Methods and Results:  Leuconostoc mesenteroides subsp. mesenteroides ATCC8293 cells were sequentially treated with penicillin G and lysozyme, and the plasmid pLeuCM was subsequently transformed into the cells. Our results demonstrated that transformation efficiencies were significantly increased (100-fold), and increased electric field strength also contributed to enhance transformation efficiency. Maximum transformation efficiency (1 × 10(4) or more transformants per μg DNA) was achieved when cells were grown in De Man, Rogosa, Sharpe (MRS) media containing 0·25 mol l(-1) sucrose and 0·8 μg ml(-1) penicillin G, followed by treatment with 600 U ml(-1) lysozyme and electroporation at a field strength of 10 kV cm(-1) . When this protocol was used to transform pLeuCM into Leuc. mesenteroides, Leuconostoc gelidum, Leuconostoc fallax and Leuconostoc argentinun, successful transformations were obtained in all cases. Furthermore, this procedure was applicable to species belonging to other genera, including Lactobacillus plantarum, Pediococcus pentosaceus and Weissella confusa. Conclusions:  The results demonstrate that the transformation efficiency for Leuconostoc spp. could be increased via optimization of the entire electroporation procedures. Significance and Impact of the Study:  These optimized conditions can be used for the extensive genetic study and the metabolic engineering of not only Leuconostoc spp. but also different species of lactic acid bacteria.
    Letters in Applied Microbiology 08/2012; 55(4):314-21. DOI:10.1111/j.1472-765X.2012.03300.x · 1.75 Impact Factor
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    ABSTRACT: Systemic inflammation has been suggested to be involved in the pathogenesis of chronic obstructive pulmonary disease (COPD) and metabolic syndrome. However, the association between these two conditions is not fully understood. To evaluate the relationship between COPD and metabolic syndrome. Among subjects aged ≥40 years from the 2001 Korean National Health and Nutrition Examination Survey, 1215 subjects with two or more acceptable spirometry measurements and complete anthropometric/laboratory examinations were analysed. A total of 133 subjects (11%, 100 men and 33 women) were newly diagnosed with COPD (forced expiratory volume in 1 second/forced vital capacity ≤ 70%). The prevalence of metabolic syndrome, based on the National Cholesterol Education Program Adult Treatment Panel III, was significantly higher in COPD subjects compared with non-COPD subjects in both sexes (33.0% vs. 22.2% in men and 48.5% vs. 29.6% in women). In men, the risk of COPD was higher in subjects with metabolic syndrome than in those without (OR 2.03, 95%CI 1.08-3.80), after adjusting for potential confounders. There was a borderline significant association between COPD and abdominal obesity among the individual component of metabolic syndrome (OR 1.95, 95%CI 0.93-4.11). In the Republic of Korea, metabolic syndrome was associated with COPD in men.
    The International Journal of Tuberculosis and Lung Disease 03/2012; 16(5):694-700. DOI:10.5588/ijtld.11.0180 · 2.76 Impact Factor
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    ABSTRACT: To examine pre-transplant risk factors for the development of post-kidney transplant tuberculosis (TB) in an intermediate TB burden country, as this is important for early detection and prophylaxis to prevent post-transplant TB. A retrospective, longitudinal cohort study of 1097 kidney transplant patients was performed at the Severance Hospital, Seoul, South Korea, between January 2000 and March 2010. The standardised incidence ratio (SIR) of post-transplant TB compared to the general population was calculated and pre-transplant risk factors were analysed. Among the 1097 kidney transplant patients, 2.1% (23/1097) developed post-transplant TB, with an incidence of 445.2 cases per 100,000 patients per year. The SIR of TB in kidney transplant patients compared with the general population was 4.26 (95%CI 2.6-6.45). A positive tuberculin skin test (TST; RR 3.54, 95%CI 1.13-11.11, P = 0.03) and previously healed TB on chest radiograph (CXR; RR 8.71, 95%CI 1.00-75.84, P = 0.05) were significant pre-transplant risk factors for post-transplant TB on multivariate analysis. The incidence of TB in kidney transplant patients was higher than in the general population. Positive TST results and previously healed TB lesions visible on pre-transplant CXR were significant pre-transplant risk factors for post-kidney transplant TB.
    The International Journal of Tuberculosis and Lung Disease 02/2012; 16(2):248-54. DOI:10.5588/ijtld.11.0259 · 2.76 Impact Factor
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    ABSTRACT: In paired living kidney exchange donation from an old donor to a young recipient, it may be argued that elderly donors provide an inferior quality kidney. However, the impact of donors older than recipients on transplant outcomes remains unclear. We retrospectively reviewed the charts of primary living kidney transplantation patients who were divided into two groups based on the age difference between donor and recipient (recipient age subtracted from donor age, donor-recipient < 20 vs ≥ 20). The donor-recipient age difference < 20 group comprised 75 and donor-recipient age difference ≥ 20 group, 25 subjects. Outcome measures included serum creatinine, acute rejection episodes as well as graft and patient survivals at 1 and 5 years after transplantation. The mean donor age difference cohorts of < 20 and ≥ 20 years showed donor ages of 33 ± 8 and 54 ± 8 years, respectively. The mean recipient age in both groups averaged under 40 years. The acute rejection rate within the first year posttransplantation was greater among age difference ≥ 20 years. The mean serum creatinine values of the donor-recipient age difference < 20 group was lower than the ≥20 years group at 1 and 5 years posttransplant. The 1-year difference was associated with an increased creatinine value at 5 years. However, death-censored graft survival of the age difference of the ≥ 20 years group was not different (hazard ratio [HR] = 0.1, 95% confidence interval [CI] = 0.01-1.37, P = .08). Patient survival of the age difference ≥ 20 years group showed no difference compared with the age difference < 20 years group (HR = 0.25, 95% CI = 0.01-6.35, P = .4). Although the cohort of a donor-young recipient age difference ≥ 20 years showed a greater risk of an acute rejection episode early posttransplantation, it did not affect graft or patient survivals. When considering paired kidney donation, older age donors should not necessarily be limited.
    Transplantation Proceedings 01/2012; 44(1):270-2. DOI:10.1016/j.transproceed.2011.12.013 · 0.95 Impact Factor
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    ABSTRACT: Previous studies identified clinical and physiologic factors of idiopathic pulmonary fibrosis (IPF) that are related to an increased risk of mortality. But there are few studies about histologic and molecular approach. We investigated whether the C-reactive protein (CRP), fibroblastic foci, phosphorylated Smad2/3 (p-Smad2/3), tumor growth factor-beta (TGF-beta), TGF-beta receptor II (TbetaRII), and the polymorphism of the TGF-beta1 codon 10 are associated with the progression of IPF patients. Eighty-six IPF patients who underwent surgical lung biopsies were examined. For each patient, clinical and physiologic parameters were investigated, and we performed immunohistochemical staining for p-Smad2/3 and TbetaRII, and genotyping of the TGF-beta1 codon 10 polymorphism. Age at diagnosis, gender, symptom duration, and smoking status did not show a significant association. However, the amount of smoking (p = 0.002), severe reduction in the percentages of predicted forced vital capacity (p = 0.013) and diffusion lung capacity of carbon monoxide (p = 0.023), CRP (p = 0.009) at diagnosis, and fibroblastic foci (p = 0.026) were associated with a poor prognosis. Cellularity, fibrosis, expression level of p-Smad2/3 and TbetaRII, and genotype of the TGF-beta1 codon 10 polymorphism did not have a statistically significant association with the prognosis. This study confirmed the amount of smoking, abrupt decrease in follow-up pulmonary function parameters, fibroblastic foci, and increased levels of CRP concentration at diagnosis were significantly associated with poor survival. Larger studies are required to confirm all prognostic factors including CRP.
    Sarcoidosis, vasculitis, and diffuse lung diseases: official journal of WASOG / World Association of Sarcoidosis and Other Granulomatous Disorders 10/2011; 28(2):102-12. · 1.74 Impact Factor
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    ABSTRACT: Tooth eruption at the early postnatal period is strictly controlled by the molecules secreted mainly from follicular tissues, which recruit monocytes for osteoclast formation. In this study, it was hypothesized that different molecules can be expressed according to the stages of tooth eruption. Rat molar germs together with follicles were extracted and DD-PCR was performed from the root formation stage 2nd molars germs (after eruptive movement) and cap stage 3rd molar germs (before movement) at postnatal day 9. Cxcl-14, a potent chemoattractant, was detected as one of the differentially expressed molecules from DD-PCR. Its expression increased significantly at the root formation stage, compared with the cap or crown formation stage at both transcription and translation levels. The expression patterns of cxcl-14 were consistent with those of MCP-1 and CSF-1, and opposite to OPG. Immunofluorescence showed that cxcl-14 was localized in the dental follicular tissues only at the root formation stage overlaying the proximo-occlusal region of the molar germs. Many osteoclasts appeared on the surface of the alveolar bone which overlayed the occlusal region of the root formation stage 2nd molar germs and underwent resorption. Cxcl-14 expression was reduced considerably at both the translation and transcription levels by an alendronate treatment. These results suggest that cxcl-14 may be implicated in the formation of the eruptive pathway of tooth germs via osteoclastogenesis.
    Journal of Experimental Zoology Part B Molecular and Developmental Evolution 09/2011; 316(6):418-26. DOI:10.1002/jez.b.21414 · 1.88 Impact Factor
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    ABSTRACT: The numbers of nationwide epidemiological surveys about chronic obstructive pulmonary disease (COPD) prevalence and prospective cohort studies for health care utilisation are limited. We investigated COPD-related health care utilisation in adults with obstructive lung disease in the second Korean National Health and Nutritional Survey (KNHANES II) in 2001 using Korean national medical insurance claim data. Among people aged >40 years, obstructive lung disease (OLD) is defined according to Global Initiative for Chronic Obstructive Lung Disease criteria. Data from a total of 1942 subjects were linked with Korean national medical insurance claims data, and we investigated their COPD-related out-patient visits from 2002 to 2005. Among the 1942 subjects, 256 (13.2%) had airflow obstruction. COPD-related out-patient visits were reported for 8.2% of patients without airway obstruction, 18.1% of those with mild airway obstruction, and 33.9% of those with moderate to very severe airway obstruction. Multivariate analysis revealed that previous COPD diagnosis by a physician (OR 2.54; P = 0.02) and lower socio-economic status (OR 0.45; P = 0.02) were independent predictors of COPD-related out-patient visits in subjects with OLD. Of the subjects with airway obstruction, those with poor financial status utilised COPD-related health care services less frequently, and those previously diagnosed as having COPD by a physician utilised the services more frequently.
    The International Journal of Tuberculosis and Lung Disease 06/2011; 15(6):824-9. DOI:10.5588/ijtld.10.0432 · 2.76 Impact Factor
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    ABSTRACT: Prominent anxiety symptoms are related to poor clinical course and outcome in major depressive disorder (MDD). The aim of this randomised, open-label, controlled study is to compare the efficacy and tolerability of mirtazapine in the form of orally disintegrating tablets against paroxetine in treating MDD patients with anxiety symptoms. A total of 60 MDD patients with a score above 18 on the Hamilton Anxiety Rating Scale (HARS) were randomly assigned to 8 weeks of fixed dosing treatment with mirtazapine (15-30 mg/day) and paroxetine (10-20 mg/day). Efficacy was primarily assessed with the HARS and with the 17-item Hamilton Depression Rating Scale (HDRS) at weeks 1, 2, 4 and 8 after treatment. Tolerability was assessed from adverse events. The generalised estimating equations (GEE) models showed that the rates of improvement in HDRS scores from baseline to week 8 were similar between mirtazapine and paroxetine groups. However, patients with mirtazapine exhibited earlier improvement in HARS scores at weeks 1 and 2. Week-by-week GEE models showed that these significant differences in improvement of HARS scores between the two treatment groups were detectable from the first evaluation after the treatment (week 1) and maintained through week 2. There was no difference in the overall frequency of adverse events experienced between the two treatment groups. The most common adverse event in the mirtazapine group was somnolence (n = 8), whereas that in the paroxetine group was gastrointestinal discomfort (n = 9). Mirtazapine and paroxetine were equally effective and well tolerated for the depressive symptoms in MDD patients with the high level of anxiety symptoms. Mirtazapine was, however, more effective in reducing the anxiety symptoms than paroxetine in the early weeks of treatment, suggesting that mirtazapine may have an earlier-onset action for the anxiety symptoms in MDD patients.
    International Journal of Clinical Practice 03/2011; 65(3):323-9. DOI:10.1111/j.1742-1241.2010.02624.x · 2.54 Impact Factor
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    ABSTRACT: This study aimed to compare the clinical efficacies of 1550- and 1565-nm Er:glass lasers in the treatment of periorbital wrinkles and to evaluate histological changes after treatment. Twenty patients received five treatments each at 3-week intervals. The right periorbital area was exposed to the 1550-nm Er:glass laser and the left periorbital area was exposed to the 1565-nm Er:glass laser. Clinical improvement was evaluated by two blinded physicians who assessed comparative photographs using a four-point scale at baseline and 3 months after the final treatments. Skin biopsies were performed in five volunteers before treatment and at 3 months after the final treatment. The mean improvement scores 3 months after treatment with the 1550- and 1565-nm Er:glass lasers were 2.25±0.62 and 2.28±0.59 respectively. Histological examination revealed increased epidermal thickening and decreased solar elastosis 3 months after the final laser treatments. Both 1550- and 1565-nm Er:glass lasers are safe and effective modalities in the treatment of periorbital wrinkles with no significant differences between the two lasers.
    Journal of the European Academy of Dermatology and Venereology 11/2010; 25(7):811-8. DOI:10.1111/j.1468-3083.2010.03870.x · 3.11 Impact Factor
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    ABSTRACT: Recent evidence suggests cathelicidin LL-37 to be a growth factor for various human cancers such as lung cancer, ovarian cancer and breast cancer. However, the effect of LL-37 against malignant skin cancer has not been reported. To investigate whether the human cathelicidin LL-37 is involved in the carcinogenesis of various skin tumours. Human cationic antimicrobial protein-18 (hCAP-18)/LL-37 production in several cell lines including HaCaT, a chronic myelogenous leukaemia (CML) cell line and various melanoma cell lines was examined using reverse transcription-polymerase chain reaction and enzyme-linked immunosorbent assay. Immunohistochemical analysis of melanoma, nonmelanoma skin cancer and precancerous and benign skin lesions was performed. After adding LL-37 to a melanoma cell line, tumour cell proliferation, migration and invasion were investigated. Human malignant melanoma cell lines overexpressed hCAP-18/LL-37 mRNA and peptide compared with HaCaT and CML cell lines. Immunohistochemistry showed that the peptide was strongly expressed in malignant melanoma and moderately expressed in squamous cell carcinoma, whereas basal cell carcinoma, precancerous lesions and seborrhoeic keratosis showed no or weak expression. LL-37 also stimulated melanoma cell proliferation, migration and invasion in vitro. Cathelicidin LL-37 was primarily expressed in human malignant skin cancer. LL-37 promoted melanoma cell proliferation, migration and invasion in vitro. We report that an increase in the level of LL-37 is associated with malignant skin tumours such as malignant melanoma. These results highlight the importance of LL-37 in the malignant tendency of skin tumours.
    British Journal of Dermatology 11/2010; 163(5):959-67. DOI:10.1111/j.1365-2133.2010.09957.x · 4.10 Impact Factor
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    ABSTRACT: Objectives: Nitric oxide synthase (NOS) are abundantly expressed in the human dental pulp (HDP) cells and a large amount of nitric oxide (NO) produced by NOS may be cytotoxic for the HDP cells. However, the cellular mechanisms underlying NO-induced cytotoxicity have not been established in HDP cells. The present study was aimed to elucidate the NO-induced cytotoxic mechanism in HDP cells. Methods: Human dental pulp (HDP) cells were obtained from tooth of patient in Chonnam National Hospital. Cell viability was examined by MTT and western blot was done for expression of Bcl-2 family, Cyt c, Fas and Fas-L. Apoptosis morphologic changes were observes by DAPI staning and Annexin V-positive cell counting. Caspases activities were assayed by spectrometer using caspase assay kits. Result: Sodium nitroprusside (SNP), a NO donor, decreased the cell viability of HDP cells in a dose- and time-dependent manner. SNP gave rise to apoptotic morphological changes, including cell shrinkage, chromatin condensation and DNA fragmentation. Besides, the number of Annexin V (a apoptotic marker)-positive cells were enhanced in SNP-treated HDP cells. SNP enhanced the production of reactive oxygen species (ROS) and N-acetylcysteine (NAC), a free radical scavenger, ameliorated the decrement of cell viability by SNP. A soluble guanylate cyclase inhibitor (ODQ) did not inhibit the decrement of cell viability by SNP. SNP augmented the amount of cytochrome c released from mitochondria to cytosol but did not affect or slightly decreased expression of Fas and Fas-L, the death receptor assemblies. In addition, SNP-treated HDP cells elevated the activities of caspase-3 and caspase-9. Moreover, SNP-treated HDP cells upregulated expression of Bax, whereas it downregulated expression of Bcl-2. Conclusions: From these results, it is speculated that NO induces apoptosis through mitochondria-dependent pathway mediated by ROS and Bcl-2 family but not by cyclic GMP pathway in HDP cells.
    IADR General Session 2010; 07/2010

Publication Stats

192 Citations
103.58 Total Impact Points

Institutions

  • 2008–2014
    • Chonnam National University
      • School of Dentistry
      Gwangju, Gwangju, South Korea
    • Yonsei University
      • • Department of Internal Medicine
      • • The Institute of Chest Diseases
      Sŏul, Seoul, South Korea
  • 2012
    • Gachon University
      • Department of Internal Medicine
      Sŏngnam, Gyeonggi Province, South Korea
    • Chungbuk National University
      • Department of Food Science and Technology
      Chinsen, North Chungcheong, South Korea
  • 2007–2012
    • Yonsei University Hospital
      • Department of Internal Medicine
      Seoul, Seoul, South Korea
  • 2000–2011
    • Seoul National University
      Sŏul, Seoul, South Korea
  • 2009
    • Korea Atomic Energy Research Institute (KAERI)
      Daiden, Daejeon, South Korea
    • Hallym University
      Sŏul, Seoul, South Korea
  • 2006
    • Changwon National University
      • Department of Industrial and Systems Engineering
      Changnyeong, South Gyeongsang, South Korea
  • 2005
    • Inje University
      • Department of Biomedical Engineering
      Kŭmhae, Gyeongsangnam-do, South Korea