Xue-qiong Wu

309th Hospital of the PLA, Peping, Beijing, China

Are you Xue-qiong Wu?

Claim your profile

Publications (10)7.14 Total impact

  • [Show abstract] [Hide abstract]
    ABSTRACT: The latency-associated antigen Rv2659c is a starvation-related protein of Mycobacterium tuberculosis (M. tuberculosis). It has potential use in tuberculosis (TB) control, but its immunological characteristics in Chinese populations are unclear. In this study, immunological characteristics and potential diagnostic use of recombinant Rv2659c protein were assessed. Interferon-γ (IFN-γ) production from peripheral blood mononuclear cells (PBMC) was assayed by enzyme-linked immunospot (ELISPOT) in TB patients (80 cases), individuals who were purified protein derivative (PPD)-positive after Bacillus Calmette-Guérin (BCG) vaccination (27 cases), nontuberculous respiratory disease patients (30 cases), individuals who were identified by standard techniques as having latent TB infection (LTBI) (37 cases), and uninfected healthy individuals (75 cases). Serum immunoglobulin G (IgG) levels were assayed by enzyme-linked immunosorbent assay (ELISA) in TB patients (43 cases), LTBI individuals (36 cases) and uninfected healthy individuals (66 cases). When stimulated by rRv2659c, PBMC from LTBI individuals gave ELISPOT counts that were significantly higher than those from TB patients, BCG vaccinated individuals, non-TB respiratory disease patients and uninfected healthy individuals (p < 0.05). The rRv2659c stimulation gave detectable IFN-γ production in a higher proportion of persons with LTBI compared with TB patients and uninfected healthy individuals. BCG vaccination and non-TB respiratory disease had little influence on the PBMC response to rRv2659c. The levels of serum IgG specific for rRv2659c were not significantly different between LTBI individuals and TB patients (p > 0.05). These results suggest that rRv2659c has potential for the diagnosis of LTBI. This is the first clinical report of human immune recognition of Rv2659c in Chinese populations.
    Journal of microbiology, immunology, and infection = Wei mian yu gan ran za zhi 04/2014; · 1.63 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Background The latency-associated antigen Rv2659c is a starvation-related protein of Mycobacterium tuberculosis (M. tuberculosis). It has potential use in tuberculosis (TB) control, but its immunological characteristics in Chinese populations are unclear. Methods In this study, immunological characteristics and potential diagnostic use of recombinant Rv2659c protein were assessed. Interferon-γ (IFN-γ) production from peripheral blood mononuclear cells (PBMC) was assayed by enzyme-linked immunospot (ELISPOT) in TB patients (80 cases), individuals who were purified protein derivative (PPD)-positive after Bacillus Calmette-Guérin (BCG) vaccination (27 cases), nontuberculous respiratory disease patients (30 cases), individuals who were identified by standard techniques as having latent TB infection (LTBI) (37 cases), and uninfected healthy individuals (75 cases). Serum immunoglobulin G (IgG) levels were assayed by enzyme-linked immunosorbent assay (ELISA) in TB patients (43 cases), LTBI individuals (36 cases) and uninfected healthy individuals (66 cases). Results When stimulated by rRv2659c, PBMC from LTBI individuals gave ELISPOT counts that were significantly higher than those from TB patients, BCG vaccinated individuals, non-TB respiratory disease patients and uninfected healthy individuals (p < 0.05). The rRv2659c stimulation gave detectable IFN-γ production in a higher proportion of persons with LTBI compared with TB patients and uninfected healthy individuals. BCG vaccination and non-TB respiratory disease had little influence on the PBMC response to rRv2659c. The levels of serum IgG specific for rRv2659c were not significantly different between LTBI individuals and TB patients (p > 0.05). Conclusion These results suggest that rRv2659c has potential for the diagnosis of LTBI. This is the first clinical report of human immune recognition of Rv2659c in Chinese populations.
    Journal of Microbiology, Immunology and Infection. 01/2014;
  • [Show abstract] [Hide abstract]
    ABSTRACT: To study the relationship between the genetic polymorphisms of carboxylesterase 1 gene (CES1) and the susceptibility to antituberculosis drug-induced hepatotoxicity (ATBDIH). Genetic polymorphisms of CES1 in 473 tuberculosis patients with or without hepatotoxicity (200:273) after antituberculosis chemotherapy were analyzed by PCR-MassArray. In 4 tags of CES1 single nucleotide polymorphism (SNP), the frequency of the rs1968753 allele had statistical difference between the hepatotoxicity group and the no-hepatotoxicity group(P = 0.0236). The characteristics of anti-hepatotoxicity had been shown relationship with rs8192950 (P = 0.044, OR = 0.649, 95%CI = 0.426 - 0.989, AC/AA) and rs1968753 (P = 0.048, OR = 0.556, 95%CI = 0.311 - 0.995, GG/AA). The diplotypes with 'CGC' haplotype exhibited significant protection against hepatotoxicity at one copy (P = 0.048, OR = 0.654, 95%CI = 0.430 - 0.996). The genetic polymorphisms of CES1 might have significant association with ATBDIH. SNP rs8192950 AC genotype and rs1968753 GG genotype might be the candidates for risk prediction of ATBDIH.
    Zhonghua nei ke za zhi [Chinese journal of internal medicine] 07/2012; 51(7):524-30.
  • [Show abstract] [Hide abstract]
    ABSTRACT: 1. The present study investigated the relationship between antituberculosis (anti-TB) drug-induced hepatotoxicity and genetic polymorphisms of two important drug-metabolizing enzymes involved in the metabolism of isoniazid, namely N-acetyltransferase 2 (NAT2) and cytochrome P450 2E1 (CYP2E1). 2. A polymerase chain reaction direct sequencing approach was used to detect genetic polymorphisms of the NAT2 and CYP2E1 genes in tuberculosis (TB) patients with (n = 101) or without (n = 107) anti-TB drug-induced hepatotoxicity. Associations between various genetic polymorphisms and anti-TB drug-induced hepatotoxicity were then determined. 3. Patients with NAT2 (282TT , 590AA and 857GA) alleles had an increased susceptibility to anti-TB drug-induced hepatotoxicity. The slow acetylator NAT2 genotypes (especially NAT2*6A/7B and NAT2*6A/6A) were risk factors for hepatotoxicity (odds ratio (OR) 9.57 (P < 0.001) for NAT2*6A/7B; OR 5.24 (P = 0.02) for NAT2*6A/6A). 4. The CYP2E1 genotype per se was not significantly associated with the development of anti-TB drug-induced hepatotoxicity. However, the combination of the CYP2E1 C1/C1 genotype with a slow acetylator NAT2 genotype increased the risk of anti-TB drug-induced hepatotoxicity (OR 5.33; P = 0.003) compared with the combination of a rapid acetylator NAT2 genotype with either a C1/C2 or C2/C2 genotype. 5. Thus, slow acetylators with the NAT2*6A/7B and NAT2*6A/6A genotypes combined with the C1/C1 CYP2E1 genotype may be involved in the pathogenesis of anti-TB drug-induced hepatotoxicity. 6. The present findings may be explained, in part, by changes in the metabolism of the anti-TB drug isoniazid induced via NAT2 and CYP2E1, a metabolic process known to produce hepatotoxic intermediates.
    Clinical and Experimental Pharmacology and Physiology 04/2012; 39(6):535-43. · 2.16 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: To study the possible relationship between polymorphic N-acetyltransferase 2 (NAT2) acetylator status and antituberculosis drug-induced hepatotoxicity and to elucidate the molecular mechanism of antituberculosis drug-induced hepatotoxicity. Blood samples from 101 tuberculosis cases with antituberculosis drug-induced hepatotoxicity and from 107 tuberculosis without antituberculosis drug-induced hepatotoxicity were collected for a case-control study. DNA of the subjects was extracted and amplified by polymerase chain reaction (PCR). The single nucleotide polymorphisms of NAT2 were determined by direct PCR sequencing. The genotype frequencies were compared between cases and controls by χ(2) test, using SPSS 12.0 software, and the association between the disease and genotypes was analyzed. Among the 101 patients with antituberculosis drug-induced liver injury, 36 patients (35.6%) were found with 282 T/T, 12 (11.9%) with 590 A/A, and 48 (47.5%) with 857 G/A or A/A. However, among the 107 controls, 9 patients (8.4%) were found with 282 T/T, 3 (2.8%) with 590 A/A, and 33 (33.8%) with 857 G/A or A/A. The patients with 282 T/T, 590 A/A, or 857 G/A or A/A genotype had a higher risk of antituberculosis drug-induced hepatotoxicity than those with 282 C/C or C/T, 590 G/G or G/A, or 857 G/G, and the OR values were 6.03 (95%CI: 2.88 - 12.62; χ(2) = 22.73, P < 0.05), 4.67 (95%CI: 1.42 - 15.44; χ(2) = 6.40, P < 0.05) and 2.03 (95%CI: 1.16 - 3.57; χ(2) = 6.08, P < 0.05) respectively. There were 40 patients with slow acetylator (39.6%) in cases with hepatotoxicity and 13 with slow acetylator (12.2%) in controls without hepatotoxicity. Patients with slow acetylator genotype (OR = 4.74, 95% CI = 2.42 - 9.28; χ(2) = 20.62, P < 0.05) had a significantly higher risk of antituberculosis drug-induced hepatotoxicity than those with rapid or intermediate acetylator genotypes. Among the cases, 19.8% (20/101) were found with NAT2(*)6A/7B, and 11.9% (12/101) with NAT2(*)6A/6A, whereas among the controls, 2.8% (3/107) were found with NAT2(*)6A/7B, and 2.8% (3/107) with NAT2(*)6A/6A respectively, the patients with NAT2(*)6A/7B and NAT2(*)6A/6A had a much higher risk of antituberculosis drug-induced hepatotoxicity, and the OR values were 8.40 (95%CI: 2.85 - 24.73; χ(2) = 14.90, P < 0.05) and 4.67 (95%CI: 1.42 - 15.44; χ(2) = 6.40, P < 0.05) respectively. Perhaps, the slow acetylation genotypes of NAT2 were the main risk factors of developing antituberculosis drug-induced hepatotoxicity.
    Zhonghua yu fang yi xue za zhi [Chinese journal of preventive medicine] 01/2011; 45(1):36-40.
  • [Show abstract] [Hide abstract]
    ABSTRACT: An oligonucleotide probe has been designed and compounded for detecting the ethambutol (EMB) resistance gene of Mycobacterium tuberculosis. It was placed on a nitrocellulose membrane for use in the reverse dot-blot hybridization assay, with the PCR product labeled with biotin. Analysis was carried out on 82 M. tuberculosis clinical isolates. The results indicate that among the 31 EMB-sensitive strains, the single-strand conformational polymorphism (SSCP) spectrum and reverse dot-blot (RDB) assay results on the embB gene in 26 strains were completely identical to those of the standard strain (H37Rv), testing positive for E1 hybridization; the SSCP spectrum of the remaining five strains indicated that migration had occurred. Among the 51 EMB-resistant strains, 24 strains tested positive for E1 hybridization; of the remaining 27 strains, 18 strains tested positive for E1b hybridization, two tested positive for E1c hybridization, five tested positive for E1d hybridization, one tested positive for E1e hybridization, and one tested positive for E1f hybridization. The low-concentration (64.70%) drug-resistant strains did not have the mutation at the codon 306 of embB. Among the 18 high-concentration drug-resistant strains, 15 strains tested positive for E1b hybridization, two tested positive for E1c hybridization, and one tested positive for E1f hybridization. The mutation detection rate was 52.94%. The RDB technology may be an easy and rapid method for detecting the codon 306 mutation of embB gene in some M. tuberculosis clinical isolates. We conclude that the ATG → GTG and ATG → CTG mutations of codon 306 of embB are one of the main causes of stron g EMB drug resistance in M. tuberculosis. Keywords Mycobacterium tuberculosis –Ethambutol–embB gene–Drug resistance–Reverse dot blot hybridization
    Annals of Microbiology 01/2011; 61(3):425-430. · 1.55 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: To evaluate the relationship between mutations in rpsL or rrs genes and streptomycin (SM) resistance, we compared four molecular methods for their clinical value in the detection of SM resistance. Genotypic analysis of SM resistance in 167 M. tuberculosis clinical strains isolated from Chinese patients was performed by direct DNA sequencing, SSCP, RFLP, and reverse dot-blot hybridization (RDBH) assays. Of the 98 SM-resistant isolates, 78 (79.6%) had missense mutations in codon 43 or 88 of rpsL resulting in a Lys to Arg substitution, 6 (6.1%) had mutations of the rrs gene at positions 513 A to C or T or 516 C to T, and 14 (14.3%) had the wild-type sequence. None of the 69 SM-susceptible isolates examined had alterations in rpsL or rrs. The results of the SSCP, RFLP, and RDBH analyses for these mutations and wild-type sequences were completely consistent with DNA sequencing data. Five distinct single-nucleotide substitutions in codon 43 or 88 of rpsL gene or in position 513 or 516 of rrs gene were correctly identified in 84 of 98 (85.7%) phenotypically SM-resistant isolates by RDBH assay. Molecular analyses of the rpsL and rrs genes are useful for rapid prediction of SM resistance in most clinical strains of M. tuberculosis. Reverse dot-blot hybridization assay is a rapid, simple, and reliable method for the detection of drug resistance.
    Acta Genetica Sinica 08/2006; 33(7):655-63.
  • Source
    Chinese medical journal 03/2006; 119(3):230-3. · 0.90 Impact Factor
  • Chinese medical journal 11/2005; 118(20):1739-41. · 0.90 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: To develop a new method, gene array, which can be used for rapid detection of rpoB mutations in Mycobacterium tuberculosis. Probes were designed according to the sequence of Mycobacterium tuberculosis rpoB gene and the gene array was developed. The DNA fragment which contained hot mutation sites of rpoB gene was amplified with biotin-labelled primers by PCR, and then hybridized with gene array. Mycobacterium tuberculosis strain H(37)Rv DNA was used as the control. The rpoB genes in Mycobacterium tuberculosis clinical isolates were also analyzed by polymerase chain reaction-single stranded conformational polymorphism (PCR-SSCP) and PCR-DNA sequencing. We analyzed the rpoB genes of 111 Mycobacterium tuberculosis clinical isolates by PCR-SSCP. Of 70 rifampicin-resistant Mycobacterium tuberculosis isolates, 63 isolates had different SSCP profiles from that of the standard strain H(37)Rv. No difference from the standard strain was found in 41 rifampicin-susceptible and 7 rifampicin-resistant isolates. We also analyzed their rpoB genes by gene array. Of 111 Mycobacterium tuberculosis clinical isolates, the results of gene array in 41 drug-sensitive strains were similar to that in Mycobacterium tuberculosis H(37)Rv. 90% (63/70) rifampicin-resistant strains had rpoB gene mutation. 53% (37/70) rifampicin-resistant strains had serine substitution at codon 531. 21% (15/70) strains had histidine substitution at codon 526. 16% (11/70) strains had amino acids substitution in other position. The results of gene array corresponded with that of PCR-SSCP and DNA sequencing. Gene array might become a rapid, simple, and accurate method for detecting rpoB mutations in most of the rifampicin-resistant Mycobacterium tuberculosis.
    Zhonghua jie he he hu xi za zhi = Zhonghua jiehe he huxi zazhi = Chinese journal of tuberculosis and respiratory diseases 06/2004; 27(5):332-5.

Publication Stats

28 Citations
7.14 Total Impact Points

Institutions

  • 2005–2014
    • 309th Hospital of the PLA
      Peping, Beijing, China
  • 2012
    • The 251st Hospital of Chinese PLA
      Chzhantseyakou, Hebei, China
  • 2006
    • Chinese PLA General Hospital (301 Hospital)
      Peping, Beijing, China