K Tervo

University of Helsinki, Helsinki, Province of Southern Finland, Finland

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Publications (50)105.15 Total impact

  • [Show abstract] [Hide abstract]
    ABSTRACT: Familial dysautonomia (Riley-Day syndrome) is characterized by both sensory and autonomic dysfunction. The authors describe Finnish twins without Jewish ancestry displaying all the criteria for the Riley-Day syndrome. The twins also had cardiological findings which have not been reported previously in connection with the Riley-Day syndrome.
    07/2009; 13(1):45-49.
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    ABSTRACT: Integrin complex α6β4 is a component of the hemidesmosome. In the unwounded cornea both the integrin subunits face the laminin-containing basement membrane, but the α6 subunit is also located between the basal cells. While the migrating epithelium is known to be without hemidesmosomes, we investigated the distribution of α6β4 during epithelial healing. Epithelial abrasion 7.5 mm in diameter was mechanically created. The rabbits were killed 1–24 h or 2, 3, or 7 days later. Monoclonal antibodies against α6, β4, and laminin A were used to detect their distributions by immunohistochemistry. Positive immunostaining for laminin A on the surface of the unepithelialized stroma indicated that basement membrane was intact after the epithelial abrasion. Three hours after corneal wounding, α6 was detectable around the entire cell up to the leading edge of the migrating epithelium. In the peripheral wound, α6 was also prominently present around the basal and suprabasal cells with only the superficial cell layers being negative. The β4 subunit showed a dissimilar distribution; it was not detectable subjacent to the leading edge. After 1 h the immunoreaction for the β4 subunit had faded 15–20 μm peripheral to the wound margin. Thereafter the subepithelial band was segmentally reassembled, starting from the periphery and progressing toward the central area of the wound. One week after epithelial wounding, immunolabeling for both integrin subunits was indistinguishable from that of the control cornea. Our results indicate that in addition to the β4 in the rabbit cornea, the α6 subunit is also complexed with another β subunit during the epithelial healing phase. The results also suggest that basal cells 15–20 μm peripheral to the wound margin disassemble their HDs prior to the migration process.
    Acta Ophthalmologica Scandinavica 01/2009; 74(1):21-25. · 1.85 Impact Factor
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    ABSTRACT: Integrin complex alpha 6 beta 4 is a component of the hemidesmosome. In the unwounded cornea both the integrin subunits face the laminin-containing basement membrane, but the alpha 6 subunit is also located between the basal cells. While the migrating epithelium is known to be without hemidesmosomes, we investigated the distribution of alpha 6 beta 4 during epithelial healing. Epithelial abrasion 7.5 mm in diameter was mechanically created. The rabbits were killed 1-24 h or 2, 3, or 7 days later. Monoclonal antibodies against alpha 6, beta 4, and laminin A were used to detect their distributions by immunohistochemistry. Positive immunostaining for laminin A on the surface of the unepithelialized stroma indicated that basement membrane was intact after the epithelial abrasion. Three hours after corneal wounding, alpha 6, was detectable around the entire cell up to the leading edge of the migrating epithelium. In the peripheral wound, alpha 6, was also prominently present around the basal and suprabasal cells with only the superficial cell layers being negative. The beta 4 subunit showed a dissimilar distribution; it was not detectable subjacent to the leading edge. After 1 h the immunoreaction for the beta 4 subunit had faded 15 - 20 microns peripheral to the wound margin. Thereafter the subepithelial band was segmentally reassembled, starting from the periphery and progressing toward the central area of the wound. One week after epithelial wounding, immunolabeling for both integrin subunits was indistinguishable from that of the control cornea. Our results indicate that in addition to the beta 4 in the rabbit cornea, the alpha 6 subunit is also complex with another beta subunit during the epithelial healing phase. The results also suggest that basal cells 15 - 20 microns peripheral to the wound margin disassemble their HDs prior to the migration process.
    Acta Ophthalmologica Scandinavica 03/1996; 74(1):21-5. · 1.85 Impact Factor
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    ABSTRACT: Extracellular matrix protein tenascin (TN) is expressed in the anterior stroma during corneal wound healing. In this study we analysed TN release in tear fluid after photorefractive keratectomy (PRK). Tear fluid TN concentrations of ten PRK patients were measured with an immunoassay. Tear fluids were collected preoperatively and 1, 2 and 7 days after PRK. The tear fluid collection time and the volume of tears collected were registered. Because tear fluid flow was greatly increased postoperatively, tear fluid flow-corrected release (TN flux) was calculated. The tear fluid flow was 4.50 +/- 0.94 microliters/min (mean +/- SEM) preoperatively, 55.48 +/- 16.70 microliters/min (P < 0.01) on the 1st, 33.91 +/- 7.91 microliters/min (P < 0.01) on the 2nd, and 13.79 +/- 5.49 microliters/min (P > 0.05) on the 7th postoperative day. The preoperative TN concentration was 0.85 +/- 0.20 microgram/ml. On the 1st postoperative day it decreased to 0.37 +/- 0.17 microgram/ml (P > 0.05), most likely due to the dilution effect caused by hypersecretion after PRK. The TN concentration was 0.67 +/- 0.12 microgram/ml (P > 0.05) on the 2nd and 0.78 +/- 0.15 micrograms/ml (P > 0.05) on the 7th postoperative day. The preoperative TN flux was 5.23 +/- 1.88 ng/min. On the 1st and 2nd postoperative days the TN flux was 14.40 +/- 4.99 ng/min (P < 0.05) and 22.66 +/- 6.12 ng/min (P < 0.05), respectively. On the 7th postoperative day a tendency towards decreased flux (14.00 +/- 6.02 ng/min, P > 0.05) was observed. Although there is a minor decrease in TN concentration after PRK due to increased tear fluid flow, a significant increase in TN flux was observed. Complete reepithelialization of the ablated area was observed in all eyes at the follow-up visit on postoperative day 7.
    Albrecht von Graæes Archiv für Ophthalmologie 08/1995; 233(8):479-83. · 1.93 Impact Factor
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    ABSTRACT: Integrins, which are composed of an alpha and beta subunit, are capable of binding to a number of extracellular matrix proteins and, hence, affect cell adhesion and proliferation. The distribution of the integrin beta (beta 1, beta 3-beta 5) and alpha (alpha 1-6 and alpha v) subunits in human anterior chamber angle was studied in eyes from subjects aged 9 months to 81 years using the indirect immunofluorescence technique. Immunoreaction for the beta 1 subunit was found throughout the trabecular meshwork (TM), in the cribriform layer, and in the endothelial lining of Schlemm's canal (SC). Labelling for the alpha 3 subunit was found in the TM and the cribriform layer only. In infant eyes the alpha 5 subunit was present in all three areas with the highest concentration in the cribriform layer, whereas no reaction was observed in adult eyes. The alpha 6 subunit was localized to the endothelium of SC only. Immunoreaction for the alpha v subunit was present in the TM and the cribriform layer of infants and young adults. The present results suggest the presence of several integrin heterodimers, acting as potential receptors for laminin, collagen, fibronectin, and vitronectin, in the anterior chamber angle.
    Albrecht von Graæes Archiv für Ophthalmologie 06/1995; 233(5):291-5. · 1.93 Impact Factor
  • T Latvala, K Tervo, T Tervo
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    ABSTRACT: We investigated the reassembly of hemidesmosomes and epithelial basement membrane (BM) following experimental excimer laser photorefractive keratectomy (PRK) immunohistochemically using monoclonal antibodies against integrin subunits alpha 6 and beta 4 (hemidesmosome components) or laminin (a BM component). The rabbits were killed 3 days, 1, 3, 6, or 12 months after a -5.0 D PRK. For untreated corneas, integrin subunits alpha 6 and beta 4 and laminin showed normal distribution at the basal aspect of the basal epithelial cells, appearing as a distinct fluorescent line. The alpha 6 subunit was also found at the basolateral membranes of the basal epithelial cells. The average healing time for the PRK wound was 6 days, but secondary epithelial defects occurred in 17 of 22 corneas. The subepithelial labeling for all the antibodies investigated showed focal discontinuities at the level of the BM in the wound area up to 12 months after PRK. After 6 months, secondary epithelial defects were very rare. In addition to the patchy subepithelial labeling, lamellar anterior stromal immunoreaction for alpha 6 and beta 4 integrin subunits was also present 1-12 months after PRK. Laminin was also observed in the anterior stroma 1-6 months after PRK. Our results suggest that focal discontinuities of hemidesmosomes or BM observed immunohistochemically in the unexpectedly slow-healing PRK wound areas might correlate with the epithelial healing problems observed in most rabbits.
    The CLAO journal: official publication of the Contact Lens Association of Ophthalmologists, Inc 05/1995; 21(2):125-9.
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    ABSTRACT: Posterior perforating eye injury carries a high risk of visual loss due to the formation of intravireal and epiretinal scar tissue. Intraocular scar formation in patients with retinal detachment has been shown to be associated with elevated intravitreal FN levels. The extracellular matrix glycoproteins fibronectin (FN) and tenascin (TN) have been located in epiretinal scar membranes. As both FN and TN are also involved in healing of cutaneous and corneal wounds, we undertook to study their expression in rabbit perforating scleral wounds with vitreous incarceration. A perforating scleral wound was produced and sutured without removal of vitreous from the wound in 18 pigmented rabbits. The rabbits were killed at various times (1 h to 21 days) after the operation, and the indirect immunohistochemical method was used for demonstration of FN and TN. Monoclonal mouse hybridoma antibodies 52 DH1 and 100 EB2, recognizing the cellular form of FN (cFN) and TN, respectively, were used. During the first post-operative week immunoreaction for glycoproteins, both the locally produced cFN and TN, were observed at the scar tissue containing the prolabed vitreous and the adjacent sclera. Subsequently, the reaction gradually shifted to the vitreal side of the wound, and 3 weeks after the operation it was almost completely restricted to a separated mass of vitreous beneath the scar. The expression of cFN and TN in the scleral scar and vitreous is indicative of their local synthesis. The shift of the expression of those proteins to the vitreal side of the wound with time suggests that the scarring process in the vitreous is delayed compared to the sclera.
    Albrecht von Graæes Archiv für Ophthalmologie 04/1995; 233(3):168-72. · 1.93 Impact Factor
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    ABSTRACT: An indirect immunohistochemical technique was used to monitor the expression of cellular fibronectin (cFN) and tenascin (TN) in the rabbit cornea after photorefractive keratectomy (PRK) in a 1 year follow up study. Rabbits received a 5.0 D myopic PRK, and were killed 3 days, 1, 3, 6, or 12 months after the operation. In most corneas, secondary epithelial defects appeared after the primary healing (mean 6.3 (SD 1.2) days). Corneal haze appeared a few weeks after PRK and was observed throughout the follow up. Three days after wounding an immunoreaction for cFN was observed as a bright narrow subepithelial line, but no immunoreaction for TN could be seen in the anterior third of the corneal stroma. However, at 1-6 months a similar location of immunoreactions for both cFN and TN was observed. Both were found in the anterior stroma at depths of 30-50 microns. At 12 months, only a trace of cFN immunoreaction but no TN immunoreaction could be discerned. Our results suggest that subepithelial scar tissue contains both cFN and TN up to 12 months.
    British Journal of Ophthalmology 02/1995; 79(1):65-9. · 2.73 Impact Factor
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    ABSTRACT: Sensory nerves known to affect corneal healing are damaged to a variable degree after photorefractive keratectomy (PRK). To search for nerve-bound factors involved in corneal healing, we monitored tear fluid calcitonin gene-related peptide (CGRP) levels of six human eyes undergoing PRK. CGRP concentrations were determined using an immunoassay. Normal human tear fluid contains CGRP. The mean CGRP concentration was slightly increased postoperatively, despite a marked tear fluid hypersecretion. Consequently, an almost ten-fold increase in release of CGRP in tears was observed on days 1 and 2 after PRK. Values measured on day 7 had returned to a normal level. The observed postoperative increase in release of CGRP in tears may have an impact on the healing of PRK wounds. Extensive neural damage following deep photoablations may impair healing and should probably be avoided.
    Journal of refractive surgery (Thorofare, N.J.: 1995) 01/1995; 11(2):126-8. · 2.47 Impact Factor
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    ABSTRACT: To study the morphological recovery of rabbit corneal nerves for up to 12 months after photorefractive keratoablation. The histochemical acetylcholinesterase reaction was used to demonstrate morphological features in rabbit corneal nerves at 1 hour, at 6 weeks, and at 3, 6, or 12 months after photorefractive keratoablation. Photorefractive keratoablation ablated the subepithelial nerve plexus, leaving sharply cut stromal nerves. Epithelial innervation was almost completely restored in 3 months, with innervation proceeding from the epithelial nerve plexuses at the wound margins, but the stromal nerves showed abnormal morphological features throughout the study. Abnormally coiled, regenerating axons emerged from the cut stromal nerves. The first of them were observed to penetrate into the epithelium 3 months postoperatively. The acetylcholinesterase reaction of the epithelial cell membranes was weaker in the wound area, except in the specimens that were observed at 12 months. Epithelial innervation was restored relatively quickly; otherwise, neural recovery was slow. The structure and architecture of the stromal nerves was abnormal even in the the specimens that were observed at 12 months. We hypothesize that corneal nerve damage induced by deep photoablations may correlate with the predictability problems and haze.
    Archives of Ophthalmology 12/1994; 112(11):1466-70. · 3.83 Impact Factor
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    ABSTRACT: An orbital nylon prosthesis was removed because of an infection caused by Staphylococcus aureus that was resistant to antimicrobials. It was processed for histopathology and immunohistochemistry. Within 3 weeks the implant had an extensive ingrowth of fibrovascular tissue containing chronic inflammatory cells, foreign body giant cells, and myofibroblasts. By using the indirect immunofluorescent method, this tissue was found to react with monoclonal antibodies (Mabs) against extradomain A of cellular fibronectin (EDA-cFN) and tenascin (TN). The presence of EDA-cFN and TN within the implant are indicative of an active healing process, since both of these proteins, scarce in adult tissues, have been shown to be reexpressed during tissue regeneration. The findings suggest that fibronectin plays a definite role in bacterial adherence and foreign body infections.
    Albrecht von Graæes Archiv für Ophthalmologie 03/1993; 231(2):61-5. · 1.93 Impact Factor
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    ABSTRACT: Integrins are heterodimeric cell-surface receptor glycoproteins involved in cell-matrix and also in cell-cell interactions. The alpha 6 beta 4 integrin heterodimer has been shown to be a component of the hemidesmosome. In response to wounding, hemidesmosomes are disassembled, the epithelium migrates to cover the denuded area, and eventually the hemidesmosomes reappear. In the present investigation the distribution of the integrin alpha 6 and beta 4 subunits after anterior keratectomy was studied by indirect immunohistochemistry. Labeling for the alpha 6 subunit was observed around the entire cell surface, right to the leading edge. The immunoreaction for the beta 4 subunit was confined to the basal cell membrane facing the basement membrane as in the normal cornea. Cells at the leading edge of the migrating epithelium did not show any labeling for beta 4. Patchy labeling for beta 4 was first observed in the region midway between the wound margin and the leading edge. Because integrins are only expressed as heterodimers, the alpha 6 subunit may be complexed with the beta 1 subunit, instead of with beta 4, at the leading edge of the migrating epithelium. We also suggest that this alpha 6 beta 1 heterodimer may play a role in the reformation of the adhesion complex.
    Cornea 12/1992; 11(6):523-8. · 1.75 Impact Factor
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    ABSTRACT: A 63-year-old man, who had for one month been on sulfasalazine therapy, developed general malaise, high fever, severe stomatitis, and bilateral necrotizing pseudomembranous conjunctivitis with corneal erosion, identical to that seen in the Stevens-Johnson syndrome. Topical therapy with antibiotics and aprotinin rapidly healed the corneal surfaces, while densely adherent true membranes developed on the conjunctiva, and were removed surgically several times during the next week. After the acute stage, subtle subepithelial conjunctival scarring, superficial punctate keratitis, dry eye syndrome and fluctuating irregular corneal astigmatism became evident, but good visual acuity, lid function and ocular motility were retained. Histopathologic study of conjunctival membranes from two cases of membranous conjunctivitis revealed polymorphonuclear leukocytes within a matrix composed of fibrin, tenascin and fibronectin. In older membranes, histiocytes were additionally found. Surgical debridement of such membranes removes a substratum of inflammatory debris that is likely to promote secondary infection, fibrosis and symblepharon formation, and may decrease rather than increase subsequent scarring of the necrotized conjunctiva.
    Acta ophthalmologica 09/1992; 70(4):534-42. · 2.44 Impact Factor
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    ABSTRACT: Wound healing is a complex, long-lasting regulatory sequence that involves expression of a number of genes, which are active during the individual's development. Some of the phenomena differ from normal tissue turnover and growth only quantitatively. This article reviews the current data on corneal wound healing, with particular reference to mesenchymal matrix proteins and their integrin receptors, to growth factors and to proteolytic enzymes. Some inflammatory mediators are also discussed. The theoretical basis for therapeutic interventions is also discussed briefly, in the light of present knowledge.
    Annals of Medicine 03/1992; 24(1):19-27. · 4.73 Impact Factor
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    ABSTRACT: Abstract Extracellular matrix (ECM) proteins form distinct proteinfamilies that play a role during tissue maturation, wound healing and maintenance of tissue architecture. Recent studies show that there are tissue type -specific variations in their expression. ECM proteins function by complexing with each other and also by interacting with their cellular receptors, called integrins. Integrins are heterodimeric membrane.glycoproteins that are partly cell type -specifically expressed in human tissues. Like other stratified epithelia, corneal epithelium expresses α2β1, α3β1, α6β1,4, and αvβ1 integrins that mediate attachment to the basement membrane and cell-cell interactions.
    Acta ophthalmologica 02/1992; 70(S202):18 - 21. · 2.44 Impact Factor
  • Acta ophthalmologica Scandinavica. Supplement 02/1992;
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    ABSTRACT: In order to investigate the effects of excimer laser surgery on corneal wound healing, 25 rabbits underwent anterior keratectomy at a depth of 100 or 300 microns with a Meditec MEL 50 excimer laser. After various intervals the animals were killed and the cornea excised and investigated immunohistochemically for the expression of extracellular matrix (ECM) proteins, fibronectin and tenascin. Fibronectin was shown to occur earlier than tenascin, and the two also had different distribution patterns. Wound depth showed no clear effect on the localization and time of ECM protein expression. This study indicates that corneal wounds caused by excimer laser radiation and those caused by mechanical surgery differ as to healing mechanisms.
    Albrecht von Graæes Archiv für Ophthalmologie 02/1992; 230(2):178-83. · 1.93 Impact Factor
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    ABSTRACT: Integrins are heterodimeric plasma membrane glycoproteins involved in cell-matrix and cell-cell interactions. The present communication reviews the distribution of several of the currently known integrin subunits in the corneal epithelium. The corneal epithelium contains the following integrin heterodimers: alpha 2 beta 1, alpha 3 beta 1, and alpha 6 beta 4. The expression of alpha v with an unknown beta subunit is also recognized, whereas the expression of alpha 4 and alpha 5 subunits remains controversial. Some of the changes occurring in the distribution of integrins in response to wounding will also be discussed.
    Acta ophthalmologica Scandinavica. Supplement 02/1992;
  • [Show abstract] [Hide abstract]
    ABSTRACT: Extracellular matrix (ECM) proteins form distinct protein families that play a role during tissue maturation, wound healing and maintenance of tissue architecture. Recent studies show that there are tissue type-specific variations in their expression. ECM proteins function by complexing with each other and also by interacting with their cellular receptors, called integrins. Integrins are heterodimeric membrane glycoproteins that are partly cell type-specifically expressed in human tissues. Like other stratified epithelia, corneal epithelium expresses alpha 2 beta 1, alpha 3 beta 1, alpha 6 beta 1, 4, and alpha v beta 1 integrins that mediate attachment to the basement membrane and cell-cell interactions.
    Acta ophthalmologica Scandinavica. Supplement 02/1992;
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    ABSTRACT: The involvement and role of the plasminogen activator-plasmin system in normal and pathological wound healing is reviewed. The methods currently available for demonstrating plasmin activity are briefly described. The article also reviews some other serine proteases potentially involved in pathological wound healing processes. The current ophthalmological therapeutic measures used to regulate tissue proteolysis are also described.
    Acta ophthalmologica Scandinavica. Supplement 02/1992;

Publication Stats

779 Citations
105.15 Total Impact Points

Institutions

  • 1978–2009
    • University of Helsinki
      • • Department of Anatomy
      • • Department of Ophthalmology
      Helsinki, Province of Southern Finland, Finland
  • 1991–1992
    • Helsinki University Central Hospital
      • Department of Ophthalmology
      Helsinki, Province of Southern Finland, Finland
  • 1985
    • University of New South Wales
      Kensington, New South Wales, Australia