W.A. van Cappellen

Publications (2)3.64 Total impact

• Article: Correction to “Advanced Level-Set-Based Cell Tracking in Time-Lapse Fluorescence Microscopy” [Mar 10 852-867]

  O. Dzyubachyk, W. A. van Cappellen, J. Essers, W. J. Niessen, E. Meijering
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  ABSTRACT: In the above titled paper (ibid., vol. 29, no. 3, pp. 852-867, Mar. 10), several figure citations were incorrect. The correct figure citations are provided here.
  IEEE Transactions on Medical Imaging 07/2010; · 3.64 Impact Factor
• Conference Proceeding: Energy minimization methods for cell motion correction and intracellular analysis in live-cell fluorescence microscopy

  O. Dzyubachyk, W.A. van Cappellen, J. Essers, W. Niessen, E. Meijering
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  ABSTRACT: The ultimate aim of many live-cell fluorescence microscopy imaging experiments is the quantitative analysis of the spatial structure and temporal behavior of intracellular objects. This requires finding the precise geometrical correspondence between the time frames for each individual cell and performing intracellular segmentation. In a previous paper we have developed a powerful multi-level-set based algorithm for automated cell segmentation and tracking of many cells in time-lapse images. In this paper, we propose approaches to exploit the output of this algorithm for the subsequent tasks of cell motion correction and intracellular segmentation. Both tasks are formulated as energy minimization problems and are solved efficiently and effectively by distance-transform and graph-cut based algorithms. The potential of the proposed approaches for intracellular analysis is demonstrated by successful experiments on biological image data showing PCNA-foci and nucleoli in HeLa cells.
  Biomedical Imaging: From Nano to Macro, 2009. ISBI '09. IEEE International Symposium on; 08/2009