Publications (1)3 Total impact
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Article: Regeneration of comparative genomic hybridization oligonucleotide microarrays with dimethylurea.
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ABSTRACT: Reuse of materials in DNA hybridization-based methods has been known since the advent of Southern membranes. Array-based comparative genomic hybridization is essentially Southern hybridization with multiple probes immobilized on a solid surface. We show that comparative genomic hybridization microarrays fabricated with maskless array synthesizer technology can be used up to four times with the application of 1,3-dimethylurea as an array-stripping agent. We reproducibly detected chromosomal aberrations (0.6-22.4Mb in size) in four hybridization rounds using regenerated microarray slides. We also demonstrated that regenerated arrays can detect smaller alterations (16-200kbp), such as common copy number variants, as well as complex aberration profiles in tumor DNA.Analytical Biochemistry 04/2012; 426(2):91-3. · 3.00 Impact Factor
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2012
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Medical University of Gdansk
- Department of Biology and Pharmaceutical Botany
Gdańsk, Pomeranian Voivodeship, Poland
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