Chariya Kaewsaneha

University of Lyon, Lyons, Rhône-Alpes, France

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Publications (11)28.38 Total impact

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    ABSTRACT: Nanoparticles possessing magnetic and fluorescent properties were fabricated by the covalent attachment of fluorescein isothiocyanate onto magnetic polymeric nanoparticles functionalized by chitosan. The synthesized magnetic polymeric nanoparticles-chitosan/fluorescein isothiocyanate were successfully used for labeling the living organ and blood-related cancer cells, i.e., HeLa, Hep G2, and K562 cells. The cytotoxicity test of nanoparticles at various incubation times indicated the high cell viability (>90%) without morphological change. The confocal microscopy revealed that they could pass through cell membrane within 2 h for K562 cells and 3 h for HeLa and Hep G2 cells and then confine inside cytoplasm of all types of tested cells for at least 24 h. Therefore, the synthesized magnetic polymeric nanoparticles-chitosan/fluorescein isothiocyanate would potentially be used as cell tracking in theranostic applications.
    Journal of Biomaterials Applications 06/2014; · 2.76 Impact Factor
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    ABSTRACT: Anisotropic polymeric colloidal or Janus particles possessing simultaneous magnetic and fluorescent properties were successfully prepared via the swelling-diffusion or the in situ emulsion polymerization method. In the swelling-diffusion process, magnetic emulsions (an organic ferrofluid dispersed in aqueous medium) were synthesized and used for seeds of submicron magnetic Janus particles. After swelling the anisotropic particles obtained by 1-pyrene-carboxaldehyde fluorescent dye dissolved in tetrahydrofuran, well-defined fluorescent-magnetic Janus particles were produced. In the in situ emulsion polymerization, styrene monomer mixed with fluorescent dye monomers, i.e., 1-pyrenylmethyl methacrylate (PyMMA) or fluorescein dimethacrylate (FDMA), and an oil-soluble initiator (2,2'-azobis(2-isobutyronitrile)) were emulsified in the presence of magnetic seed emulsions. The confocal microscopic images showed the fluorescent-magnetic Janus particles with high fluorescent intensity when a fluorescent crosslinker monomer FDMA was employed.
    Journal of Colloid and Interface Science 06/2014; 424:98–103. · 3.55 Impact Factor
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    ABSTRACT: Discovery of the magnetic property (superparamagnetism) of ferrofluid (i.e., magnetic fluid) and its effects on the other materials open wide varieties of applications in human daily life. Continually, new applications require different forms of magnetic materials, which have significant attractions nowadays. This review highlights the ferrofluids synthesis methods. All known methods are cited, and a special interest is focused on the most commonly used methods for preparation, which depends on size reduction and chemical co-precipitation. Furthermore, the industrial and biomedical applications of ferrofluids and their based particles are discussed. Finally, the new water based dispersed ferrofluids particles “magnetic emulsion” is also introduced.
    Journal of Colloid Science and Biotechnology. 03/2014; 3(1).
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    ABSTRACT: Submicron magnetic polymeric particle (MPP) and/or Janus magnetic polymeric particle (JMPP) were successfully prepared via the miniemulsion polymerization of styrene (St)/acrylic acid (AA) monomers consisting of superparamagnetic iron oxide nanoparticles (IONPs) coated with oleic acid. Since the particle nucleation occurs primarily within the monomer droplet, not only the size of particle could be controlled at the beginning but also its morphology was manipulated by using different types of initiator. The MPPs with homogeneous distribution of IONPs (41%) in P(St-DVB-AA) were obtained with using potassium persulfate (KPS) as initiator and divinyl benzene (DVB) as crosslinking agent. To obtain anisotropic JMPP, an oil-soluble initiator 2,2-azobis (2-isobutyronitrile) was used instead. The controllable phase separation between P(St-AA) and the encapsulated IONPs caused the stable spherical Janus particles containing IOPNs (15%) located on one side of polymer particle. Both MPPs and JMPPs could be easily separated by an external magnet. The MPPs were functionalized with chitosan (CS) acting as spacer and then chemical immobilized with fluorescein isothiocyanate (FITC) to produce fluorescent-MPPs. When applying as imaging device for cancer cells labeling i.e., HeLa, cells, results showed that MPPs/CS-FITC could be located inside cells with low cytotoxicity.
    Advanced Materials Research. 02/2014; 893:329-336.
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    ABSTRACT: Janus colloidal particles (JCPs), compartmentalized colloids with two sides of different chemistry or polarity, have attracted significant attention in recent years due to their novel morphologies and diverse potential applications in materials science, biomedicine and in the field of highly specific biosensors. Due to these fascinating features, the synthesis of Janus particles remains a big challenge. However, major progress concerning their preparation in useful amounts has been achieved in recent years. Among the used synthetic approaches, Pickering emulsion, bearing solid nanoparticles at the interface between two liquid phases as the stabilizing agent, is one of the elegant methods used for the synthesis of Janus colloidal particles with controllable morphology in a wide range of size and surface functionality. A monolayer of organic and inorganic nanoparticles can stabilize an emulsion droplet only when their hemispherical surface is chemically modified, while the remaining surface is protected. This approach offers the possibility to alter the surface of nanoparticles with a variety of functional groups which lead to Janus particles with complex structure.In this review article, we represent an overview on the state of the art for producing Janus colloidal particles based on Pickering emulsion strategy. The pioneer and recent respective works of Janus colloidal particles with anisotropic in surface and/or in structure were also described.
    Colloids and Surfaces A Physicochemical and Engineering Aspects 12/2013; 439:35-42. · 2.35 Impact Factor
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    ABSTRACT: Anisotropic Janus magnetic polymeric nanoparticles are prepared via the miniemulsion polymerization of styrene and acrylic acid monomers in the presence of oleic acid-coated magnetic nanoparticles (MNPs). The controllable phase separation between the polymer matrix and the encapsulated MNPs is a key success factor to produce Janus morphology. The effects of MNPs, 2,2′-azobis(2-isobutyronitrile) and sodium dodecyl sulfate contents, on the morphology, chemical composition and colloidal stability of the prepared Janus hybrid particles are investigated. Besides the determination of polymerization conversion, zeta potential, size analysis, TGA, and TEM are applied for characterization of the anisotropic particles. The results show the stable spherical Janus particles containing MNPs (15 wt % magnetic content) located on one side of each polymer particle. The anisotropic submicron Janus magnetic polymeric particles (250 nm) can be easily separated by an external magnet. © 2013 Wiley Periodicals, Inc. J. Polym. Sci., Part A: Polym. Chem. 2013, 51, 4779–4785
    Journal of Polymer Science Part A Polymer Chemistry 11/2013; 51(22). · 3.54 Impact Factor
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    ABSTRACT: Submicron hybrid magnetic, polystyrene (PS) based Janus particles containing magnetic nanoparticles (MNPs) on one side were successfully assembled using the miniemulsion/solvent evaporation method. Nanodroplets of styrene (St) monomer in the presence of PS and MNPs were generated in an aqueous continuous phase. The subsequent evaporation of St monomers resulted the precipitation of PS and MNPs into spherical nanoparticles. The effect of PS content, oil:water phase ratio, MNPs:PS ratio, and type and concentration of stabilizers on Janus morphology was investigated. A MNPs:PS weight ratio of 1:1 promoted the formation of Janus-like particles with MNPs located on one side due to the increased PS concentration during evaporation of the monomer solvent. Of the stabilizing agents tested (sodium dodecylsulfate (SDS), Triton X-405, polyvinyl alcohol, and Pluronic F-68), the presence of 1.0gL(-1) SDS and 20.0gL(-1) Pluronic F-68 was found to effectively stabilize hybrid particles with Janus morphology.
    Journal of Colloid and Interface Science 08/2013; · 3.55 Impact Factor
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    ABSTRACT: Janus or anisotropic colloidal particles comprising of at least two components of different chemistry, functionality and/or polarity have attracted attentions in a wide range of applications, e.g. in optics, magnetics, plasmonics, colloidal chemistry, and biomedicine. The interesting features of Janus colloidal particles are attributed to their tunable and controllable asymmetric structure, which allows controlling their physico-chemical properties, down to the nanoscale. Moreover, their synergistic potential for multiplexing, multi-level targeting, and combination therapies make them particularly attractive for biomedical applications. However, the synthesis of Janus colloidal particles must be well adapted to get particles with precise control of their various structural/physical/chemical properties. Nowadays, the advance in new fabrication processes is a strong need for fabricating compact composite particles with spatially separated functionalities, uniform size, tunable composition, and effective response to stimuli. In this review article, we summarized the most recent representative works on Janus colloidal particles including the various fabrication methods, important properties, and their potential applications, particularly in the biomedical field.
    ACS Applied Materials & Interfaces 02/2013; · 5.90 Impact Factor
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    ABSTRACT: The nanoparticle with simultaneous combination of magnetic and fluorescent properties was prepared by immobilization of fluorescein isothiocyanate (FITC) onto magnetic polymeric nanoparticle (MPNP). The MPNP with 41% magnetic content was obtained from incorporating Fe(3)O(4) magnetic nanoparticles (MNPs) into poly(styrene/divinyl benzene/acrylic acid) via the miniemulsion polymerization. Before labeling with FITC, the carboxylated MPNP was coated with chitosan (CS) having low, medium, or high molecular weight (MW) in order to avoid quenching of the fluorescent by iron oxide. Data obtained from TEM, size and zeta potential measurements clearly indicated the presence of CS as a shell surrounding the superparamagnetic MPNP core. The zeta potential, FTIR, and fluorescent spectroscopies confirmed the attachment of FITC to the MPNP-CS via covalent bonding. The higher MW or longer chains of CS (300kDa) offered the larger spacer with multiple sites for the FITC binding and, thus, provided the higher fluorescent emission intensity. The MPNP-CS immobilized with FITC would be useful for cell-labeling application.
    Journal of Colloid and Interface Science 03/2012; 377(1):145-52. · 3.55 Impact Factor
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    ABSTRACT: Skim latex, a by-product of natural rubber latex, was prevulcanized by sulfur and then used in the preparation of composite particles with disinfectant nanocapsules. Each polymeric nanocapsule contained chlorhexidine digluconate (CHD) as a disinfectant agent encapsulated with poly(methyl acrylate) (PMA) as a shell. The driving force for the heterocoagulation of CHD-PMA nanocapsule and sulfur-prevulcanized skim (SPVS) particle was the electrostatic interaction between the positive charge of lecithin residing on the surface of the nanocapsule and the negative charge from the indigenous surfactant (protein-lipid) and/or from sodium dodecyl sulfate on the surface of the SPVS latex particle. The zeta potential and morphology of heterocoagulated particles indicated the formation of the CHD-PMA/SPVS composite particles. Structures and formation mechanisms of the composite particles with different blend ratios were revealed by depth profiling confocal Raman spectra.
    International Journal of Polymer Analysis and Characterization 11/2010; 15(8):524-535. · 1.49 Impact Factor
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    ABSTRACT: Sulphur-prevulcanised skim (SPVS) rubber particles were heterocoagulated on an aggregate of a disinfectant agent (chlorhexidine digluconate; CHD)-poly(methyl acrylate) (PMA) nanocapsules. The CHD-PMA nanocapsule stabilized by lecithin was prepared using the modified nanoprecipitation of PMA from an organic continuous phase onto nanodroplet containing the CHD aqueous solution. Cationic and anionic nanocapsules were produced by separately redispersing the nanocapsules into a water and 0.5% sodium dodecyl sulphate (SDS) aqueous solution, respectively. The largest aggregate, which was obtained from homocoagulation of the CHD-PMA nanocapsules with opposite charges, was selected as a core surrounded with SPVS shell particles. An interpolymer complex between poly(ethylene oxide) (PEO) moieties adsorbed on the aggregate and indigenous surfactant (protein-lipid) and/or SDS on the SPVS particle surface was the driving force for heterocoagulation. Data from the size, zeta potential measurements and TEM images of the heterocoagulated CHD-PMA/SPVS indicated the composite particle containing the CHD-PMA core. Keywordsheterocoagulation-capsule-composite-skim-nanoparticle
    Macromolecular Research 09/2010; 18(9):876-883. · 1.68 Impact Factor