-
[show abstract]
[hide abstract]
ABSTRACT: Proline (Pro) plays a versatile role in cell metabolism and physiology. Pro and hydroxypro are major imino acids present in collagen, an important connective tissue protein, essential for wound healing, which is a primary response to tissue injury. This study explains the role of L-pro on cutaneous wound healing in rats when administered both topically and orally. Open excision wounds were made on the back of rats, and 200 μl (200 mg) of pro was administered topically and orally once daily to the experimental rats until the wounds healed completely. The control wounds were left untreated. Granulation tissues formed were removed after day 4 and 8 of post excision wounding, and biochemical parameters such as total protein, collagen, hexosamine, and uronic acid were estimated. Levels of enzymatic and non-enzymatic antioxidants such as catalase, superoxide dismutase, glutathione peroxidase, ascorbic acid, and reduced glutathione were evaluated along with lipid peroxides in the granulation tissues. Tensile strength and period of epithelialization were also measured. It was observed that the treated wounds healed very fast as evidenced by augmented rates of epithelialization and wound contraction, which was also confirmed by histological examinations. The results strappingly authenticate the beneficial effects of the topical administration of L-proline in the acceleration of wound healing than the oral administration and control.
Amino Acids 03/2013; · 3.25 Impact Factor
-
[show abstract]
[hide abstract]
ABSTRACT: Rapid synthesis of mono-dispersed gold nanoparticles through economically feasible green chemistry approach is highly desirable. In this study, we have developed a method to synthesize mono-dispersed gold nanoparticles (PAuNPs) by mixing gold solution with fruit peel extract of Punica granutum without using any surfactant or external energy. In this method, physiologically stable, biocompatible PAuNPs were formed within 60s. Casein, being a biocompatible polymer, is used to couple the prepared PAuNPs for functionalization of folic acid, which is highly expressed in cancer cells. These functionalized PAuNPs could be used for targeted drug delivery for cancer with enhanced therapeutic efficacy and minimal side effects. PAuNPs were characterized by UV, IR, TEM, Particle size analyzer and zeta potential measurement. In vitro stability of the PAuNPs was also analyzed. Hemocompatibility of PAuNPs was evaluated in human blood samples and found that the particles were hemocompatible. The toxicity of the PAuNPs, 5-Fu and 5Fu@PAuNPs was analyzed in zebrafish embryos. The in vitro cytotoxicity of free 5-Fu, 5Fu@PAuNPs-Fa was investigated against MCF-7 cells (breast cancer) and observed that the amount of 5-Fu required to achieve 50% of growth of inhibition (Ic(50)) was much lower when compared to free 5-Fu.
Colloids and surfaces. B, Biointerfaces 01/2013; 106C:208-216. · 2.60 Impact Factor
-
[show abstract]
[hide abstract]
ABSTRACT: Hollow amphiphilic carbon nanosphere loaded insulin with biodegradable polymer coating
(ACP) for oral delivery, was developed to overcome intestinal epithelial barriers and protect insulin from photolytic enzymes. ACP was characterized by laser diffraction spectroscopy for size, distribution and shape by using transmission electron microscopy. Drug – carrier
compatibility was studied individually by FTIR and CD spectroscopy. Insulin encapsulation
efficiency and in vitro release were determined by Bradford protein assay. Availability of
bioactivity was determined using Wistar diabetic rats. The mean size of ACP ~ was 330 nm
with poly index value of Insulin encapsulation efficiency was 93.35%. We observed that
ACP formulation suppressed insulin release in acidic media and promoted a sustained release
at near neutral conditions. Hence, ACP insulin could be an alternative for injections to
diabetic patients.
Colloids and surfaces. B, Biointerfaces 11/2012; · 2.60 Impact Factor
-
[show abstract]
[hide abstract]
ABSTRACT: Hollow amphiphilic carbon nanosphere loaded insulin with biodegradable polymer coating (ACP) for oral delivery, was developed to overcome intestinal epithelial barriers and protect insulin from photolytic enzymes. ACP was characterized by laser diffraction spectroscopy for size, distribution and shape by using transmission electron microscopy. Drug-carrier compatibility was studied individually by FTIR and CD spectroscopy. Insulin encapsulation efficiency and in vitro release were determined by Bradford protein assay. Anti-diabetic activity of ACP loaded insulin was determined using Wistar diabetic rats. The mean size of ACP was ∼330nm with poly index value of 1. Insulin encapsulation efficiency was 93.35%. We observed that ACP formulation suppressed insulin release in acidic media and promoted a sustained release at near neutral conditions. Hence, ACP insulin could be an alternative for injections to diabetic patients.
Colloids and surfaces. B, Biointerfaces 11/2012; 103C:238-243. · 2.60 Impact Factor
-
[show abstract]
[hide abstract]
ABSTRACT: Acalypha indica Linn. (Acalypha indica) vernacularly called Kuppaimeni in Tamil, has been used as a folklore medicine since ages for the treatment of wounds by tribal people of Tamil Nadu, Southern India. The present study investigates the biochemical and molecular rationale behind the healing potential of Acalypha indica on dermal wounds in rats.
Acalypha indica extract (40 mg/kg body weight) was applied topically once a day on full-thickness excision wounds created on rats. The wound tissue was removed and used for estimation of various biochemical and biophysical analyses and to observe histopathological changes with and with-out extract treatment. The serum levels of pro-inflammatory cytokine tumor necrosis factor (TNF-α) was measured at 12 h, 24 h, 48 h and 72 h post-wounding using ELISA. Reverse transcription-polymerase chain reaction (RT-PCR) analysis was performed to study the expression pattern of transforming growth factor [TGF-β1], collagen 1 α (I) [Col 1 α (I)] and collagen 3 α (I) [Col 3 α (I)]. Likewise, linear incision wounds were created and treated with the extract and used for tensile strength measurements.
Wound healing in control rats was characterized by less inflammatory cell infiltration, lack of granulation tissue formation, deficit of collagen and significant decrease in biomechanical strength of wounds. Acalypha indica treatment mitigated the oxidative stress and decreased lipid peroxidation with concomitant increase in ascorbic acid levels. It also improved cellular proliferation, increased TNF-α levels during early stages of wound healing, up-regulated TGF-β1 and elevated collagen synthesis by markedly increasing the expression of Col 1 α (I) and Col 3 α (I). Increased rates of wound contraction, epithelialization, enhanced shrinkage temperature and high tensile strength were observed in the extract treated rats.
Acalypha indica extract was shown to augment the process of dermal wound healing by its ability to increase collagen synthesis through up-regulation of key players in different phases of wound healing and by its antioxidative potential.
Journal of ethnopharmacology 04/2012; 142(1):14-22. · 2.32 Impact Factor
-
[show abstract]
[hide abstract]
ABSTRACT: Ethnopharmacological relevance: Acalypha indica Linn. (A. indica) vernacularly called
Kuppaimeni in Tamil, has been used as a folklore medicine since ages for the treatment
of wounds by tribal people of Tamil Nadu, Southern India. The present study investigates
the biochemical and molecular rationale behind the healing potential of A.indica on
dermal wounds in rats.
Material and methods: A. indica extract (40 mg/kg body weight) was applied topically
once a day on full-thickness excision wounds created on rats. The wound tissue was
removed and used for estimation of various biochemical and biophysical analyses and to
observe histopathological changes with and with-out extract treatment. The serum levels
of pro-inflammatory cytokine tumor necrosis factor (TNF-α) was measured at 12 h, 24 h,
48 h and 72 h post wounding using ELISA. Reverse transcription-polymerase chain
reaction (RT- PCR) analysis was performed to study the expression pattern of
transforming growth factor [TGF-β1], collagen 1 α (I) [Col 1 α (I)] and collagen 3 α (I)
[Col 3 α (I)]. Likewise, linear incision wounds were created and treated with the extract
and used for tensile strength measurements.
Results: Wound healing in control rats was characterized by less inflammatory cell
infiltration, lack of granulation tissue formation, deficit of collagen and significant
decrease in biomechanical strength of wounds. A. indica treatment mitigated the
oxidative stress and decreased lipid peroxidation with concomitant increase in ascorbic
acid levels. It also improved cellular proliferation, increased TNF-α levels during early
stages of wound healing, up-regulated TGF-β1 and elevated collagen synthesis by
markedly increasing the expression of Col 1 α (I) and Col 3 α (I). Increased rates of
3
wound contraction, epithelialization, enhanced shrinkage temperature and high tensile
strength were observed in the extract treated rats.
Conclusion: A. indica extract was shown to augment the process of dermal wound
healing by its ability to increase collagen synthesis through up-regulation of key players
in different phases of wound healing and by its antioxidative potential.
Journal of Ethnopharmacology 04/2012; · 3.01 Impact Factor
-
[show abstract]
[hide abstract]
ABSTRACT: Preservation or curing of hides and skins is performed as the primary step of leather processing. Common salt is employed as the conventional agent for curing purpose. Use of salt enhances the pollution load of tannery effluent which becomes highly contaminated with increased total Dissolved Solids (TDS) and chlorides (Cl). To overcome this hurdle, researchers are in constant search of alternative preservation techniques which are either totally void of salt or use only a meager amount of salt. In the present study, we had explored the possibility of using Semecarpusanacardium (S. anacardium) nutextract as an alternative to salt for the curing process by assessing different parameters like hair slip, putrefaction odor, volatile nitrogen content, moisture content, bacterial count and shrinkage temperature in comparison to the salt curing method. The antibacterial property of the plant extract was also investigated. The results obtained, substantiated that the nutextract of S. anacardium effectively preserve the skins for more than a month, by its antibacterial activity along with the dehydrating property of acetone.
Journal of Cleaner Production 03/2012; · 2.73 Impact Factor
-
[show abstract]
[hide abstract]
ABSTRACT: In this preliminary investigation, we have studied the toxicity of A.squamosa extract which has been reported for its numerous medicinal properties. An
attempt has been carried out to evaluate the toxicity of the crude extract in zebrafish before using it for animal studies. Ethanolic extract of A.squamosa (100
μg – 1000 μg) was administered to screen the toxicity in zebrafish model. Only higher concentrations (900 μg and 1000 μg) showed considerable mortality
and delay in hatching. Overall, there was no significant toxicity observed in zebrafish development when compared to control zebrafish. This will help us
to predetermine the dosage and toxicity of A.squamosa for further studies in rat model system.
Journal of Pharmacy Research. 12/2011; 5(1-ISSN: 0974-6943):277-279.
