Brahatheeswaran Dhandayuthapani

Publications (3)3.48 Total impact

• Article: Biocompatible nanofibers based on extremophilic bacterial polysaccharide, Mauran from Halomonas maura.

  Sreejith Raveendran, Brahatheeswaran Dhandayuthapani, Yutaka Nagaoka, Yasuhiko Yoshida, Toru Maekawa, D Sakthi Kumar
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  ABSTRACT: Extremophilic bacterial polysaccharide based biocompatible nanofibers were produced for the first time via electrospinning technique. Mauran (MR), an extremophilic sulfated exopolysaccharide was extracted from moderately halophilic bacterium, Halomonas maura and characterized for the application of nanofiber synthesis. Thin-uniform MR nanofibers were produced using homogenous solutions of poly (vinyl alcohol) (PVA) blended with different concentrations of MR. Characterization of complex MR/PVA nanofibers were performed using scanning electron microscope and analyzed for the cytotoxicity using mouse fibroblast cells as well as mesenchymal stem cells. An average of 120nm sized nanofibers were produced and tested for an enhanced cell growth under in vitro conditions in comparison with control. MR and MR/PVA nanofibers were found to be an excellent biomaterial for the migration, proliferation and differentiation of mammalian cells, which was confirmed by cell adhesion studies and confocal microcopy. Interestingly, biological and physicochemical properties of MR hasten the application of MR based nanofibers for various biomedical applications like tissue engineering and drug delivery.
  Carbohydrate polymers. 02/2013; 92(2):1225-33.
• Source

  Available from: Aby Cheruvathoor Poulose

  Article: Biomimetic smart nanocomposite: in vitro biological evaluation of zein electrospun fluorescent nanofiber encapsulated CdS quantum dots.

  Brahatheeswaran Dhandayuthapani, Aby Cheruvathoor Poulose, Yutaka Nagaoka, Takashi Hasumura, Yasuhiko Yoshida, Toru Maekawa, D Sakthi Kumar
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  ABSTRACT: New hybrid quantum dot (QD)/nanofibers have potential applications in a variety of fields. A novel fluorescent nanocomposite nanofiber material, consisting of CdS and zein has been fabricated through the electrospinning process. A detailed optimization was carried out to fabricate continuous and uniform nanofibers without beads or droplets. The synthesized hybrid nanofibers were characterized by various state-of-the-art techniques such as scanning electron microscopy, transmission electron microscopy (TEM), TEM-energy dispersive spectrometry, atomic force microscopy and confocal fluorescence micrography. The optimization process was carried out to fabricate fibers ranging from 200 to 450 nm in diameter. The electrical conductivity of the zein-CdS hybrid nanofiber substrates was tested. The potential use of the electrospun CdS-encapsulated nanofibrous scaffold as substrates for cell/tissue culture was evaluated with two different cell types, i.e. mesenchymal stem cells and fibroblasts. The results showed that the electrospun fibrous scaffolds could support the attachment and the proliferation of cells. In addition, the cells cultured on the fibrous scaffolds exhibited normal cell shapes and integrated well with surrounding fibers. The obtained results confirmed the potential for the use of the electrospun QD-encapsulated fluorescent nanofiber mats as scaffolds for tissue engineering.
  Biofabrication 05/2012; 4(2):025008. · 3.48 Impact Factor
• Source

  Available from: PubMed Central

  Article: Evaluation of Antithrombogenicity and Hydrophilicity on Zein-SWCNT Electrospun Fibrous Nanocomposite Scaffolds.

  Brahatheeswaran Dhandayuthapani, Saino Hanna Varghese, Ravindran Girija Aswathy, Yasuhiko Yoshida, Toru Maekawa, D Sakthikumar
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  ABSTRACT: Design of blood compatible surfaces is required to minimize platelet surface interactions and increase the thromboresistance of foreign surfaces when they are used as biomaterials especially for artificial blood prostheses. In this study, single wall carbon nanotubes (SWCNTs) and Zein fibrous nanocomposite scaffolds were fabricated by electrospinning and evaluated its antithrombogenicity and hydrophilicity. The uniform and highly smooth nanofibers of Zein composited with different SWCNTs content (ranging from 0.2 wt% to 1 wt%) were successfully prepared by electrospinning method without the occurrence of bead defects. The resulting fiber diameters were in the range of 100-300 nm without any beads. Composite nanofibers with and without SWCNT were characterized through a variety of methods including scanning electron microscopy, transmission electron microscopy, thermogravimetric analysis, and tensile mechanical testing. The water uptake and retention ability of composite scaffolds decreased whereas thermal stability increased with an addition of SWCNTs. Hemolytic property and platelet adhesion ability of the nanocomposite (Zein-SWCNTs) were explored. These observations suggest that the novel Zein-SWCNTs composite scaffolds may possibly hold great promises as useful antithrombotic material and promising biomaterials for tissue engineering application.
  International Journal of Biomaterials 01/2012; 2012:345029.