Y. Randhir Babu Singh

Regional Institute of Medical Sciences, Imphal, Manipur, India

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Publications (3)0.4 Total impact

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    ABSTRACT: The in vitro antifungal activities of azole drugs viz., itraconazole, voriconazole, ketoconazole, econazole and clotrimazole were investigated in order to evaluate their efficacy against filamentous fungi isolated from mycotic keratitis. The specimen collection was carried out from fungal keratitis patients attending Aravind eye hospital and Post-graduate institute of ophthalmology, Coimbatore, India and was subsequently processed for the isolation of fungi. The dilutions of antifungal drugs were prepared in RPMI 1640 medium. Minimum inhibitory concentrations (MICs) were determined and MIC50 and MIC90 were calculated for each drug tested. A total of 60 fungal isolates were identified as Fusarium spp. (n=30), non-sporulating moulds (n=9), Aspergillus flavus (n=6), Bipolaris spp. (n=6), Exserohilum spp. (n=4), Curvularia spp. (n=3), Alternaria spp. (n=1) and Exophiala spp. (n=1). The MICs of ketoconazole, clotrimazole, voriconazole, econazole and itraconazole for all the fungal isolates ranged between 16μg/mL and 0.03μg/mL, 4μg/mL and 0.015μg/mL, 8μg/mL and 0.015μg/mL, 8μg/mL and 0.015μg/mL and 32μg/mL and 0.06μg/mL respectively. From the MIC50 and MIC90 values, it could be deciphered that in the present study, clotrimazole was more active against the test isolates at lower concentrations (0.12-5μg/mL) when compared to other drugs tested. The results suggest that amongst the tested azole drugs, clotrimazole followed by voriconazole and econazole had lower MICs against moulds isolated from mycotic keratitis. Copyright © 2014 Elsevier Masson SAS. All rights reserved.
    Journal de Mycologie Médicale/Journal of Medical Mycology 12/2014; 25(1). DOI:10.1016/j.mycmed.2014.10.024 · 0.40 Impact Factor
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    ABSTRACT: Fusarium spp. are the most frequently isolated causative agents of keratitis in South India. Due to the great number of antifungal resistant Fusaria, new, alternative therapeutic agents are extremely needed. Essential oils and their compounds are considered as potential antifungal agents. The aim of this study was to investigate the antifungal effect of 9 essential oils (Cinnamomum zeylanicum, Citrus limon, Eucalyptus citriodora, Gaultheria procumbens, Juniperus communis, Melaleuca alternifolia, Origanum majoranna, Salvia sclarea and Thymus vulgaris oil) and their combination with antifungals in broth microdilution test against the most common etiologic agents of Fusarium keratitis in South India. The metabolic inhibition effect of the most active oil and its component was investigated by FUN-1 staining. The lowest minimal inhibitory concentration values were observed in the case of C. zeylanicum oil (CZO) and its main component, trans-cinnamaldehyde (tCA). Whereas, the C. limon oil was the least effective. Interaction tests revealed mostly no interaction between tCA and natamycin. FUN-1 staining revealed that CZO and tCA cause reduced cellular metabolism. According to our results CZO and tCA could be potentially used in the treatment of Fusarium keratitis. The research of M.H., Cs.V. and L.G. was supported by the European Union and the State of Hungary, co-financed by the European Social Fund in the framework of TÁMOP 4.2.4.A/2-11-1-2012-0001 'National Excellence Program'. The relating research groups were also supported by the INSA-HAS interacademic bilateral project (SNK-49/2013) providing infrastructure and research equipment.
    Ecoforum 2014; 09/2014
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    24th European Congress of Clinical Microbiology and Infectious Diseases (ECCMID); 05/2014

Publication Stats

1 Citation
0.40 Total Impact Points

Institutions

  • 2014
    • Regional Institute of Medical Sciences
      • Department of Microbiology
      Imphal, Manipur, India
    • Dr G R Damodaran college of Science
      Koyambattūr, Tamil Nadu, India
    • Dr. G. R. Damodaran College of Science, Coimbatore
      Koyambattūr, Tamil Nadu, India