[Show abstract][Hide abstract] ABSTRACT:
Fusarium spp. are the most frequently isolated causative agents of keratitis in South India. Due to the great number of antifungal resistant Fusaria, new, alternative therapeutic agents are extremely needed. Essential oils and their compounds are considered as potential antifungal agents.
The aim of this study was to investigate the antifungal effect of 9 essential oils (Cinnamomum zeylanicum, Citrus limon, Eucalyptus citriodora, Gaultheria procumbens, Juniperus communis, Melaleuca alternifolia, Origanum majoranna, Salvia sclarea and Thymus vulgaris oil) and their combination with antifungals in broth microdilution test against the most common etiologic agents of Fusarium keratitis in South India. The metabolic inhibition effect of the most active oil and its component was investigated by FUN-1 staining.
The lowest minimal inhibitory concentration values were observed in the case of C. zeylanicum oil (CZO) and its main component, trans-cinnamaldehyde (tCA). Whereas, the C. limon oil was the least effective. Interaction tests revealed mostly no interaction between tCA and natamycin. FUN-1 staining revealed that CZO and tCA cause reduced cellular metabolism.
According to our results CZO and tCA could be potentially used in the treatment of Fusarium keratitis.
The research of M.H., Cs.V. and L.G. was supported by the European Union and the State of Hungary, co-financed by the European Social Fund in the framework of TÁMOP 4.2.4.A/2-11-1-2012-0001 'National Excellence Program'. The relating research groups were also supported by the INSA-HAS interacademic bilateral project (SNK-49/2013) providing infrastructure and research equipment.