P Jalc

Slovak Academy of Sciences, Bratislava, Bratislavsky Kraj, Slovakia

Are you P Jalc?

Claim your profile

Publications (20)27.22 Total impact

  • [Show abstract] [Hide abstract]
    ABSTRACT: 1. The effect of hemisection of the cervical spinal cord on NADPH-diaphorase staining in the reticular nuclei of the rabbit medulla was investigated using histochemical technique. 2. A quantitative assessment of somal and neuropil NADPH-diaphorase staining was made by an image analyzer in a selected area of each reticular nucleus of the rabbit medulla. 3. On the 7th postsurgery day, the highest up-regulation of somatic NADPH-diaphorase staining was observed in regions regulating cardiorespiratory processes; however, the highest increase of neuropil NADPH-diaphorase staining was found in the reticular nuclei modulating the tonus of postural muscles. 4. The degeneration of non-NADPH-diaphorase-stained neurons was detected throughout the reticular formation of the medulla, but the extent of neuronal death did not correlate with the up-regulation of the NADPH-diaphorase staining in the reticular nuclei of the medulla. 5. The findings provide evidence that NADPH-diaphorase-exhibiting neurons are refractory to the hemisection of the cervical spinal cord and that the neuronal up-regulation of NADPH-diaphorase at the medullar level is probably not a causative factor leading to the death of the reticulospinal neurons.
    Cellular and Molecular Neurobiology 01/2005; 24(6):865-72. · 2.29 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Changes in the level of cyclic 3',5'-guanosine monophosphate (cGMP) were studied one day after a surgically induced spinal cord constriction performed at the Th7 segment level in the dorsal, lateral and ventral white matter columns and in the non-compartmentalized white matter of Th5-Th6 segments, i.e., above the site of the spinal cord constriction and in Th8-Th9 segments, located below the spinal cord constriction. The midthoracic spinal cord constriction caused a significant decrease in the level of cGMP in the ventral column of Th5-Th6 segments and a significant increase in the lateral column of Th8-Th9 segments. The level of cGMP in the dorsal column, located either rostrally or caudally to the site of the spinal cord injury, remained unchanged. In addition, no significant changes in the level of cGMP were found in the non-compartmentalized white matter of Th5-Th6 and Th8-Th9 segments in response to constriction of the Th7 segment.
    Neurochemistry International 11/2001; 39(4):275-82. · 2.66 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Changes in the level of cyclic 3′,5′-guanosine monophosphate (cGMP) were studied one day after a surgically induced spinal cord constriction performed at the Th7 segment level in the dorsal, lateral and ventral white matter columns and in the non-compartmentalized white matter of Th5–Th6 segments, i.e., above the site of the spinal cord constriction and in Th8–Th9 segments, located below the spinal cord constriction. The midthoracic spinal cord constriction caused a significant decrease in the level of cGMP in the ventral column of Th5–Th6 segments and a significant increase in the lateral column of Th8–Th9 segments. The level of cGMP in the dorsal column, located either rostrally or caudally to the site of the spinal cord injury, remained unchanged. In addition, no significant changes in the level of cGMP were found in the non-compartmentalized white matter of Th5–Th6 and Th8–Th9 segments in response to constriction of the Th7 segment.
    Neurochemistry International. 10/2001;
  • [Show abstract] [Hide abstract]
    ABSTRACT: The distribution and changes of catalytic nitric oxid synthase (cNOS) activity in the dorsal, lateral and ventral white matter columns at midthoracic level of the rabbit's spinal cord were studied in a model of surgically-induced spinal cord constriction performed at Th7 segment level and compared with the occurrence of nicotinamide adenine dinucleotide phosphate diaphorase expressing and neuronal nitric oxide synthase immunoreactive axons in the white matter of the control thoracic segments. Segmental and white-column dependent differences of cNOS activity were found in the dorsal (141.5 +/- 4.2 dpm/microm protein), lateral (87.3 +/- 11.5 dpm/microm protein) and ventral (117.1 +/- 7.6 dpm/microm protein) white matter columns in the Th5-Th6 segments and in the dorsal (103.3 +/- 15.5 dpm/microm protein), lateral (54.9 +/- 4.9 dpm/microm protein), and ventral (86.1 +/- 6.8 dpm/microm protein) white matter columns in the Th8-Th9 segments. A surgically-induced constriction of Th7 segment caused a disproportionate response of cNOS activity in the rostrally (Th5-Th6) and caudally (Th8-Th9) located segments in both lateral and ventral white matter columns. While a statistically significant decrease of cNOS activity was detected above the constriction site in the ventral columns, a considerable, statistically significant increase of cNOS activity was noted in the white lateral columns below the site of constriction. It is reasoned that the changes of cNOS activity may have adverse effects on nitric oxide (NO) production in the white matter close to the site of constriction injury, thus broadening the scope of the secondary mechanisms that play a role in neuronal trauma.
    Neurochemical Research 09/2000; 25(8):1139-48. · 2.13 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Segmental and laminar distribution of Fos-like immunoreactive, reduced nicotinamide adenine dinucleotide phosphate diaphorase (NADPHd)-exhibiting and double-labeled (Fos-like immunoreactive and NADPHd-exhibiting) neurons was examined in lower lumbar and sacral segments of the dog spinal cord using the model of multiple cauda equina constrictions. NADPHd histochemistry was used as marker of nitric oxide synthase-containing neurons. The appearance and the time-course of Fos-like immunoreactive, NADPHd and double-labeled neurons was studied at 2 h and 8 h postconstriction characterized as the incipient phase of cauda equina syndrome. The occurrence of Fos-like immunoreactive and NADPHd-exhibiting neurons in fully developed cauda equina syndrome was studied at five days postconstriction. An increase in Fos-like immunoreactivity in superficial laminae (I-II) and an enhanced NADPHd staining of lamina VIII neurons were found. A statistically significant increase in Fos-like immunoreactive neurons was found in laminae I-II and VIII-X 8 h postconstriction, and in contrast, a prominent decrease in Fos-like immunoreactive neurons was found in laminae I-II, accompanied by a statistically significant increase in Fos-like immunoreactive neurons in more ventrally located laminae VII-X at five days postconstriction. Quantitative analysis of laminar distribution of constriction-induced NADPHd-exhibiting neurons revealed a considerable increase in these neurons in laminae VIII-IX 8 h postconstriction and a statistically highly significant increase in NADPHd-exhibiting neurons in laminae VII-X five days postconstriction. Concurrently, the number of NADPHd-exhibiting neurons in laminae I-II was greatly reduced. While a low number of double-labeled neurons was found throughout the gray matter of lower lumbar and sacral segments at 2 h postconstriction, a statistically significant number of double-labeled neurons was found in lamina X 8 h and in laminae VII-X five days postconstriction. The course and distribution of anterograde degeneration resulting five days after multiple cauda equina constrictions are compared with segmental and laminar distribution of Fos-like immunoreactive and NADPHd-exhibiting neurons. Prominent involvement of the spinal cord neurons appearing in the lumbosacral segments at the early beginning and in fully developed cauda equina syndrome results in a Fos-like immunoreactivity and strongly enhanced NADPHd staining of some neuronal pools. Under such circumstances, an early cauda equina decompression surgery is advisable aimed at decreasing or preventing the derangement of the neural circuits in the lumbosacral segments.
    Neuroscience 02/2000; 95(2):543-57. · 3.12 Impact Factor
  • Source
    J Marsala, P Jalc
    [Show abstract] [Hide abstract]
    ABSTRACT: The aim of this study was the histochemical characterization of NADPH diaphorase-positive neuronal pools in the rabbit lumbosacral segments using a model of single, repeated and multiple sublethal spinal cord ischemia. Following a single 8-min sublethal spinal cord ischemia and 1-hour reperfusion, the staining of NADPH diaphorase-exhibiting neurons in the dorsal horn, pericentral region, dorsal gray commissure and sacral parasympathetic nucleus was comparable with the control sections. In contrast to the foregoing sublethal ischemia, a regionally different somatic NADPH diaphorase (NADPHd) staining was found after multiple sublethal spinal cord ischemia. Whereas an almost complete loss of the staining of large NADPHd-exhibiting somata in the pericentral region was detected, the staining of the NADPHd-exhibiting neuronal pools in the deep dorsal horn and sacral parasympathetic nucleus was fully preserved. Concomitantly, a prominent reduction of small NADPH diaphorase-positive neurons was noted in the superficial dorsal horn layers of lower lumbar and sacral segments.
    Physiological research / Academia Scientiarum Bohemoslovaca 02/2000; 49(1):157-65. · 1.53 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: The distribution and changes of catalytic nitric oxid synthase (cNOS) activity in the dorsal, lateral and ventral white matter columns at midthoracic level of the rabbit''s spinal cord were studied in a model of surgically-induced spinal cord constriction performed at Th7 segment level and compared with the occurrence of nicotinamide adenine dinucleotide phosphate diaphorase expressing and neuronal nitric oxide synthase immunoreactive axons in the white matter of the control thoracic segments. Segmental and white-column dependent differences of cNOS activity were found in the dorsal (141.5 4.2 dpm/m protein), lateral (87.3 11.5 dpm/m protein) and ventral (117.1 7.6 dpm/m protein) white matter columns in the Th5-Th6 segments and in the dorsal (103.3 15.5 dpm/m protein), lateral (54.9 4.9 dpm/m protein), and ventral (86.1 6.8 dpm/m protein) white matter columns in the Th8-Th9 segments. A surgically-induced constriction of Th7 segment caused a disproportionate response of cNOS activity in the rostrally (Th5-Th6) and caudally (Th8-Th9) located segments in both lateral and ventral white matter columns. While a statistically significant decrease of cNOS activity was detected above the constriction site in the ventral columns, a considerable, statistically significant increase of cNOS activity was noted in the white lateral columns below the site of constriction. It is reasoned that the changes of cNOS activity may have adverse effects on nitric oxide (NO) production in the white matter close to the site of constriction injury, thus broadening the scope of the secondary mechanisms that play a role in neuronal trauma.
    Neurochemical Research 01/2000; 25(8):1139-1148. · 2.13 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: The distributions of neuronal nitric oxide synthase-immunoreactive neurons and of nicotinamide adenine dinucleotide phosphate-diaphorase activity were studied in the C6, Th2, L1, L5, S2 and S3 segments and laminae in the rabbit spinal cord and compared with the catalytic nitric oxide synthase activity, determined by monitoring the conversion of [3H]arginine to [3H]citrulline in the same segments and laminae. Morphologically, a heterogeneous population of nicotinamide adenine dinucleotide phosphate-diaphorase-expressing and neuronal nitric oxide synthase-immunoreactive neurons was detected in the superficial and deep dorsal horn and the pericentral region in all segments studied, and in the intermediolateral cell column of the thoracic and lumbosacral segments. A disproportionate distribution of both neuronal categories which had a significantly higher number of nicotinamide adenine dinucleotide phosphate-diaphorase-expressing rather than neuronal nitric oxide synthase-immunoreactive cell bodies was found in all segments. The catalytic nitric oxide synthase activity was distributed unequally in the C6, Th2, L1, L5, S2 and S3 segments, with a comparatively low value in the Th2 segment (70 +/- 5.1 d.p.m./microg protein) in comparison with the S3 segment, where the highest level (140 +/- 5.5 d.p.m./microg protein) was found. A close correlation between the number of neuronal nitric oxide synthase-immunoreactive somata and catalytic nitric oxide synthase activity was revealed in the dorsal horn (laminae I-VI). Whereas a low number of neuronal nitric oxide synthase-immunoreactive somata in laminae VII-X was found in the L5, S2 and S3 segments, the values of catalytic nitric oxide synthase activity in the same laminae and segments were found to be exceedingly high. These findings indicate that the occurrence of many neuronal nitric oxide synthase-immunoreactive fibers (mainly axons), and dense, punctate, non-somatic neuronal nitric oxide synthase immunopositivity in the neuropil staining of the same laminae and segments, can substantially enhance catalytic nitric oxide synthase activity.
    Neuroscience 01/1999; 94(1):229-37. · 3.12 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: The distribution of somatic, fibre-like and punctate, non-somatic reduced nicotinamide adenine dinucleotide phosphate (NADPH) diaphorase activity was examined in dog spinal cord using horizontal, sagittal and transverse sections. The morphological features of NADPH diaphorase exhibiting neurons divided into six different neuronal types (N1-N6) were described and their laminar distribution specified. Major cell groups were identified in the superficial dorsal horn and around the central canal at all spinal levels, and in the intermediolateral cell column at thoracic level. NADPH diaphorase exhibiting neurons of the pericentral region were distributed in a thin subependymal cell column containing longitudinally-arranged small bipolar neurons with processes penetrating deeply into the intermediolateral cell column and/or running rostrocaudally in the subependymal layer. The second pericentral cell column located more laterally in lamina X contains large, intensely-stained NADPH diaphorase exhibiting neurons with long dendrites radiating in the transverse plane. Neurons of the sacral parasympathetic nucleus seen in segments S1-S3 exhibited prominent NADPH diaphorase activity accompanied by heavily-stained fibres extending from Lissauer's tract through lamina I along the lateral edge of the dorsal horn to lamina V. A massive dorsal gray commissure, with high NADPH diaphorase activity, was found in segments S1-S3. At the same segmental level a prominent group of moderately-stained motoneurons was detected in the dorsolateral portion of the anterior horn. Fibre-like NADPH diaphorase activity was found in the superficial dorsal horn and pericentral region in all segments studied. Punctate, non-somatic NADPH diaphorase activity was detected in the superficial dorsal horn, in the pericentral region all along the rostrocaudal axis and in the nucleus phrenicus (segments C4-C5), nucleus dorsalis (segments Th2-L2), nucleus Y (segments S1-S3), and the dorsal part of the dorsal gray commissure (S1-S3). A schematic diagram documenting the segmental and laminar distribution of NADPH diaphorase activity is given.
    Neuroscience 09/1998; 85(3):847-62. · 3.12 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Ischemia-reperfusion induced changes in concentration of phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylserine (PS), phosphatidylinositol (PI) and sphingomyelin (SM) in the gray matter taken in toto, white matter, dorsal horns, intermediate zone and ventral horns of the rabbit's spinal cord were studied and compared with neurohistopathological changes. With the exception of PI concentration in the dorsal horns, ischemia of 25 min caused significant degradation of all phospholipids. While short-lasting recirculation (1 h) did not returned the levels of phospholipids to control values, postischemic recirculation for 3 h sharply increased the resynthesis of all phospholipids, but only the concentration of PE, PS, and PI in the dorsal horns and PC in the intermediate zone significantly improved and returned close to control values. Corresponding neurohistopathological changes resulting after the same reperfusion periods are given.
    Neurochemical Research 09/1998; 23(8):1069-77. · 2.13 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Degradation of membrane bound phospholipids in CNS during ischaemia begins with extreme rapidity. Sublethal ischaemia influences ischaemic tolerance in the affected neurons and is stressful enough to induce neuronal changes such as postischaemic hypoperfusion, transient suppression of protein synthesis and induction of stress (HSP) proteins. It seems, that the nature of factors responsible for ischaemic tolerance may involve the activation of multiple different systems. The aim of this study was to investigate the changes of phospholipids in gray matter regions of spinal cord following sublethal ischaemia repeated in long intervals of reperfusion. Male rabbits, weight range 2.5-3.5 kg were used in the experiment. They were divided in following groups : 1. control animals; 2. animals subjected to 25 min ischaemia; 3. animals subjected to 25 min ischaemia and 3 h of reperfusion; 4. animals subjected to sublethal (8-8-9 min) ischaemia repeated in long-lasting (8-8-24 h) intervals of reperfusion. Phospholipids were separated by thin layer chromatography, lipidic phosphorus was assessed spectrophotometrically. Sublethal ischaemia repeated in long-lasting intervals of reperfusion increased the concentration of phospholipids to control levels in all gray matter regions. The resynthesis in the dorsal horns, of PC and PE in the ventral horns and of PC in the intermediate zone. An excessive renewal of phospholipids after sublethal ischaemia repeated in longer intervals of reperfusion was most pronounced in the eh dorsal horns of the spinal cord and can be the result of many defensive cellular mechanisms.
    Bratislavske lekarske listy 08/1998; 99(7):359-63. · 0.47 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Neuropathological changes of the neuronal pools and spinal cord circuitry in the lumbosacral segments were studied in a canine model of multiple protracted cauda equina constrictions. Anterograde degeneration of all sacrococcygeal and L7 dorsal root fibers was detected in S1-S3 and lower lumbar segments. A narrow degenerated gracile fascicle was found in all thoracic and cervical segments terminating in the gracile nucleus. Transneuronal degeneration of middle-sized and large neurons, located in S1-S3 and sporadically in L7 segments, was noted. Identical transneuronal degeneration was seen in a group of small neurons located in the ventralmost part of lamina VII in S1-S3 segments. Simultaneously, a terminal degeneration was detected in the lateral cervical nucleus and in the ventral posterior lateral nucleus of the ventrobasal thalamic complex. Concomitantly, a fully developed retrograde degeneration affecting motoneurons in the ventrolateral portion of the anterior horn in S1-S3 segments appeared.
    Neuroscience Letters 07/1995; 193(2):97-100. · 2.03 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: The visualization of Ca binding in the myelinated axons of lumbosacral segments of rabbit was done at the electron microscopic level using the spinal cord ischemia model. To assess the calcium accumulation, the binding agent pyroantimonate was used. Nonsignificant Ca2+ binding was found in the myelinated axons after 40 min of ischemia followed immediately by perfusion fixation. A high concentration of calcium pyroantimonate deposits, seen as electron dense particles, was detected in the myelin interlamellar clefts and axoplasm. The paranodal region was the most affected site.
    Molecular and Chemical Neuropathology 01/1995; 25(2-3):143-53.
  • [Show abstract] [Hide abstract]
    ABSTRACT: Mapping the canine lumbosacral spinal cord neurons damaged by ischemia-reperfusion after high thoracic aorta ligation was performed using the Nauta degenerating method. Highly Nauta-positive perikarya of the long ascending projection systems in the 4th to 6th dorsal layer, interneurons in the 7th layer and motoneurons in the 8th and 9th layers in L3-S3 segments subjected to 30 min of ischemia and 30 min of reperfusion were localized and their laminar distribution was specified. Spastic paraplegia fully developed 2 days postoperatively after 30 min of aortic ligation is neurohistopathologically characterized by occurrence of enlarged Nauta-positive boutons with prevailing localization in the 4th to 8th layer of the gray matter.
    Neuroscience Letters 12/1989; 106(1-2):83-8. · 2.03 Impact Factor
  • P Jalc, J Marsala, M Marsala
    [Show abstract] [Hide abstract]
    ABSTRACT: The effect of 40-min ligation of the abdominal aorta and 60-min recirculation was investigated with the use of pyroantimonate. All the motoneurons were markedly damaged. Vacuolized mitochondria and basal membrane of capillaries contained calcium deposits.
    Neir̆ofiziologiia = Neurophysiology 02/1989; 21(2):267-9.
  • [Show abstract] [Hide abstract]
    ABSTRACT: Seven dogs were subjected 30 min to ligation of the thoracic aorta and were then kept alive 6-7 days after the ligature had been removed. Their spinal cord and brain stem were treated by the Nauta-Gygax method and the extent and appearance of preterminal and terminal degeneration of certain ascending spinal systems were analysed. In the medulla oblongata region, marked degenerating fibres from the lower thoracic and lumbosacral cord segments were found in the nucleus tractus spinalis nervi trigemini. Preterminal and terminal degenerating fibres were visualized in the caudal part of the trigeminal nuclear complex. Comparison with the literature showed these to be previously unknown projections with a relationship to the nucleus tractus spinalis nervi trigemini.
    Folia morphologica 02/1989; 37(1):64-70. · 0.47 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: The partial ischemia of the adult dogs, both males and females was induced by the ligature of aorta abdominalis above the branching of arteria coeliaca within 80 minutes and 120 minutes duration with the following preparation of L6 und L7 dorsal root ganglia. The nuclei and nucleoli of the dorsal root ganglia nerve cells were studied by the usual standard transmission electron microscopic procedures. Comparing the control and ischemized material we have observed the different reaction of the nuclei and nucleoli to the ischemia. Some of these structures remained unchanged, the others demonstrated a various degree of the alteration. After the partial ischemia some nucleoli were more compact, some nucleolonemata were less distinct but no karyoplasmic changes were noted. The nucleolar interstitial vacuoles were occasionally enlarged and the satellite bodies were observed scarcely in the proximity of the nucleolus.
    Journal für Hirnforschung 02/1983; 24(3):273-80.
  • Neuropatologia polska 02/1983; 21(2):315-21.
  • A Fercáková, P Jalc
    [Show abstract] [Hide abstract]
    ABSTRACT: The influence of preischemic treatment with N-acetylcysteine (NAC) in a rat model of 30 min global brain ischemia was studied. Neuronal changes in is-chemic and preischemically NAC-treated animals after 3 days survival were evaluated by light and electron microscopy. Severe ischemic injury was found in the population of small B-cells showing heavy cytoplas-mic vacuolation. Preischemic administration of NAC substantially reduced the occurrence of degenerating small neurons. Morphological changes in trigeminal ganglion have been studied following a short period of focal ischemia due to 1-7 min compression of the ganglion in the cat (2) and rabbit (6). This procedure results in an ischemic and mechanical lesion of the rootlets and trigeminal ganglion cells. There is a groving body of evidence that formation of free radicals during is-chemia and early recirculation is involved in the de-velopment of reperfusion neuronal injury (3). It has been shown that using the free radical scavengers, such as superoxide dismutase, enhanced neuronal survival in some models of cerebral ischemia. Recently, it has been reported that N-acetylcystein (NAC) can amelio-rate brain damage after transient brain ischemia (4). In this study we evaluated the influence of preis-chemic NAC treatment on reperfusion alterations in trigeminal ganglion neurons after global brain is-chemia. Adult rats were subjected to 30 min global brain ischemia using the model of 4-vessel occlusion (7) followed by 3 days recirculation. In another group of animals a single dose of NAC (400 mg/kg i. p.) was administered 30 min before the onset of ischemia. After transcardial perfusion with 4% paraformalde-hyde trigeminal ganglia were removed and processed for electron microscopy. Light microscopic examination (1 fim sections) in ischemic animals with 3 days survival revealed an in-creased number of large A-type and small B-type chro-matolytic neurons with eccentric nuclei. Numerous B-cells showed heavy vacuolation (14.5%). In rats with NAC pretreatment the occurrence of vacuolated cells was significantly reduced (7.4%). Ultrastructural al-terations in A-cells manifested by perinuclear chroma-tolysis, dark nuclei, formation of vacuoles and myeli-noid bodies. The mitochondria were well preserved. Small B-cells were more severely affected showing signs of degeneration with an extensive mitochondrial vacuolation. Preischemic NAC treatment markedly re-duced the presence of degenerating B-cells in compar-ison with non-treated animals. It is supposed that the mechanism of protective influence of NAC lies in enhancing of glutathion scav-enging system (1). There are also other possibilities which may account for NAC neuroprotective effect. Ischemic/reperfusion injury substantially impaires mi-crovascular and endothelial function that may be ameliorated by NAC due to inhibition of endothelial and neutrophils interaction (5). The results obtained demonstrate that NAC administration appear to be an efficient intervention which can reduce ischemic al-terations of neurons in the both cell populations and may have a value in the treatment of some cerebrovas-cular diseases.
  • [Show abstract] [Hide abstract]
    ABSTRACT: Changes in the concentration of membrane-bound phospholipids following single (25-min) spinal cord ischemia and 3 h of reperfusion were determined. These were compared with the changes following brief repeated (8-, 8-, and 9-min) ischemia followed each time by reperfusion for 1 h, or the same periods of ischemia followed by 8 h, 8 h, and 24 h of reperfusion, respectively. Phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylserine (PS), phosphatidylinositol (PI), and sphingomyelin (SM) were assayed in regions of the spinal cord of the rabbit, including gray matter, white matter, dorsal horns, intermediate zone, and ventral horns. The brief repeated ischemia with 1-h reperfusions produced more extensive degradation of phospholipids in almost all regions compared with the equivalent time of ischemia (25 min) in a single period. After a lengthy reperfusion after repeated ischemia, the phospholipids were resynthesized with the exception of the phosphatidylinositol in the gray matter. The resynthesis was most pronounced in the dorsal horns and in the white matter.
    Molecular and Chemical Neuropathology 35(1-3):61-76.

Publication Stats

148 Citations
27.22 Total Impact Points

Institutions

  • 1989–2001
    • Slovak Academy of Sciences
      • Institute of Neurobiology
      Bratislava, Bratislavsky Kraj, Slovakia
  • 1998
    • Parazitologický ústav SAV
      Presburg, Bratislavský, Slovakia