Mitsuro Chiba

Akita General Hospital, Akita, Akita, Japan

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Publications (23)82.58 Total impact

  • Health 01/2014; 06(10):969-974. DOI:10.4236/health.2014.610122 · 2.10 Impact Factor
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    ABSTRACT: Crohn's disease (CD) is a chronic inflammatory bowel disease (IBD) of unknown etiology. We aimed to identify the etiological agent of CD using a molecular cloning strategy that was particularly focused on identifying agents causing immune abnormalities and infectious agents. We constructed a cDNA library derived from the inflamed intestinal tissue of a CD patient, and screened 1.5 million clones in this library with the serum from another typical CD patient. The expressed cDNA clones that positively reacted with the serum were then expressed as fusion proteins with glutathione S-transferase, and western blotting was performed using the sera of 22 CD, 13 ulcerative colitis (UC), and 16 non-IBD patients. We identified nine positive clones that did not contain any viral or bacterial genomic DNA. Of these, we selected one clone (clone 50) with which the typical CD patient's serum most strongly reacted. Clone 50 is highly homologous to the antioxidant protein peroxiredoxin 6. In western blotting, the sera of 47.6% CD patients (small intestine type 80%, large and small intestine type 43%, large intestine type 0%) showed strong reactivity to clone 50, none of the UC patients were reactive to clone 50, and 18.8% of non-IBD patients were very weakly reactive to it. We also found that the expression of peroxiredoxin 6 was significantly increased in inflamed intestinal epithelia of CD. The present study first showed that some CD patients have an antibody against peroxiredoxin 6-like protein, which may be involved in the pathogenesis of CD.
    Journal of Gastroenterology and Hepatology 04/2012; 27(8):1388-94. DOI:10.1111/j.1440-1746.2012.07147.x · 3.33 Impact Factor
  • Journal of Gastroenterology and Hepatology 07/2008; 16(8):951 - 952. DOI:10.1111/j.1440-1746.2001.2379f.x · 3.33 Impact Factor
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    ABSTRACT: An unusual form of aphthoid colitis is presented. A 21 -year-old man complained of hematochezia for about 2 years. Examinations revealed crowded polypoid lesions in the lower rectum: the closer to the anus the site was, the bigger the size of the polypoid lesions. Polypoid lesions were also observed in the terminal ileum and on the ileocecal valve. Biopsy specimens revealed that these polypoid lesions were lymph follicles. Lymphoid hyperplasia was observed in the upper rectum and proximal colon but not in the transverse colon. The patient was given a salicylazosulfapyridine suppository followed by a rapid ceasing of hematochezia on the following day and disappearance of most lymph noduli in the rectum by the second week. About two years later hematochezia recurred. The findings of the lower rectum and the response to the suppository were the same as in the first episode. Known diseases associated with aphthoid colitis were ruled out.The present case is unusual in several respects compared to typical apthoid colitis: the clinical course is not acute self-limited but chronic protracted; the lesions are distributed not evenly in the large bowel or its segment(s) but in the lower rectum, in addition, the lesions become more pronounced toward the anus; skipped lymph noduli are observed in the ileocecal valve and the terminal ileum. The other characteristics of this case include a marked response to salicylazosulfapyridine, recurrence, no precedence of flu-like syndromes, no symptoms except for hematochezia, and normal laboratory data.
    Digestive Endoscopy 08/2007; 6(3):258 - 264. DOI:10.1111/j.1443-1661.1994.tb00376.x · 1.61 Impact Factor
  • Gastroenterology 04/2000; 118(4). DOI:10.1016/S0016-5085(00)83484-8 · 12.82 Impact Factor
  • Gastroenterology 04/2000; 118(4). DOI:10.1016/S0016-5085(00)81259-7 · 12.82 Impact Factor
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    ABSTRACT: ABSTRACT— A 67-year-old man with a portal-systemic shunt confirmed by three-dimensional computed tomography (3D-CT) was successfully treated by percutaneous vascular embolization. The patient had aggravated loss of memory, disorientation, and hyperammonemia. A gastrorenal shunt 16 mm in diameter was found by 3D-CT reconstructed by helical computed tomography (CT). Embolization was performed only in the shunt percutaneously through the inferior vena cava. One year after the embolization, no recurrence of portal-systemic encephalopathy and no portal hypertension have appeared, and the clinical course has been good.
    Liver International 05/1998; 18(3):208 - 212. DOI:10.1111/j.1600-0676.1998.tb00152.x
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    ABSTRACT: In ballooned hepatocytes associated with alcoholic liver disease, secretory proteins are abundant, possibly due to an impairment of the microtubule-dependent vesicular transport system. We studied the effects of ethanol and acetaldehyde on microtubules and hepatic kinesin function. Tubulin and microtubule-associated protein 2 was purified by phosphocellulose and hydroxyapatite column chromatography from microtubule proteins of bovine brain. Hepatic kinesin was purified from rabbit liver. The assembly of microtubules was quantified by spectro-photometer. The kinesin motility assay is done with or without various concentrations of ethanol or acetaldehyde using video-enhanced differential interference contrast microscopy. Ethanol had no effect on the assembly or disassembly of the microtubules or on hepatic kinesin function, even at high concentrations of up to 100 mM. In contrast, acetaldehyde reduced hepatic kinesin function at concentrations greater than or equal to 100 μM and induced microtubular disassembly at concentrations greater than or equal to 200 μM. In alcoholic liver injury, chronic reduction of hepatic kinesin function may cause retention of secretory proteins and hepatocyte swelling. Moreover, reduction of the microtubule concentrations due to acetaldehyde may further impair the vesicular transport system and reduce the cells ability to maintain morphology, in turn accelerating hepatocyte ballooning.
    Hepatology Research 01/1998; 12(3):186-197. DOI:10.1016/S1386-6346(98)00060-6 · 2.22 Impact Factor
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    ABSTRACT: The relationship between the lymph follicle and expression of HLA-DR antigens on the colonic epithelium was investigated. Colonic specimens containing lymph follicles were examined immunohistochemically for HLA-DR expression. Thirty-six biopsy specimens (29 from macroscopically uninvolved areas and seven from aphthoid lesions) were obtained from 10 patients with Crohn's disease, and another 43 specimens were obtained from 33 patients with various intestinal diseases (non-Crohn's disease). HLA-DR antigens were expressed on the epithelium around the lymph follicle in both groups. However, the rate of expression (31/36) in Crohn's disease was significantly higher than that (5/43) in non-Crohn's diseases (P
    Digestive Diseases and Sciences 12/1993; 39(1):83-90. DOI:10.1007/BF02090065 · 2.55 Impact Factor
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    ABSTRACT: Lymphoid cell subsets, including T cells as well as Ig-containing cells in the colonic mucosa and HLA-DR antigens on colonie epithelia, were examined in non-IBD colitis (colitis excluding ulcerative colitis (UC) and Crohn’s disease) by the indirect immunoperoxidase staining method. Mouse anti-CD5, CD8, CD4, IgG, IgAl, IgA2, IgM, IgD, IgE, HLA-DR, and Nula monoclonal antibodies were used as the first antibody. The results were compared to those of the normal controls and UC. T cell subsets in non-IBD colitis were almost similar to those of the controls and UC. The number of Ig-containing cells of all classes, except for IgA, tended to be increased in non-IBD colitis. In particular, both IgG- and IgE-containing cells were significantly increased compared to those in the controls. Compared to UC, IgG-containing cells were decreased in non-IBD colitis. Namely, in non-IBD colitis, as well as in UC, the change of Ig-containing cells (B cell lineage) was more pronounced than that of T cells. The frequency of the expression of HLA-DR antigens on colonie epithelia in non-IBD colitis was 70%, which was significantly higher than that in controls (0%), but significantly lower than that in UC (100%). Whether the differences in the number of IgG-containing cells, and the frequency of epithelial HLA-DR expression between non-IBD colitis and UC was due to the differences of the degree of local inflammation or due to the differences of the nature of the two diseases was not elucidated in this study.
    Journal of Gastroenterology 11/1990; 25(6):700-707. DOI:10.1007/BF02779183 · 4.02 Impact Factor
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    ABSTRACT: : Six cases of rectal leiomyoma, measuring less than 10 mm in diameter, are reported. The color of the overlying mucosa was similar to that of the surrounding mucosa. No central depression or ulceration was observed endoscopically in any of the six cases. In five cases, the tumors were sessile or semipedunculated and mobile, and were thus snare polypectomised. In the remaining case, the tumor was flat-elevated and immobile, and removed surgically by transanal wedge-resection. All six cases were benign leiomyoinas without malignant changes such as cellular atypia or mitosis.
    Digestive Endoscopy 07/1990; 2(3):323-326. DOI:10.1111/j.1443-1661.1990.tb00058.x · 1.61 Impact Factor
  • Gastroenterologia Japonica 08/1989; 24(4). DOI:10.1007/BF02774359
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    ABSTRACT: A case of BehÇet’s disease associated with amyloidosis is reported. A 34 year-old woman was referred to our Department after suffering diarrhea for one month. She had the complete type of BehÇet’s disease which began development when she was 12 years old. Diarrhea disappeared after she was admitted to the hospital but stool occult blood was strongly positive. A barium enema revealed the disappearance of haustration in the left colon. Urinalysis was normal. She suffered from cholecystolithiasis, and a cholecystectomy was performed. A histopathological examination revealed amyloid deposits in the stomach, duodenum, large bowel, gallbladder, and kidney. Amyloid was indicated as AA type after treating kidney and colon sections with potassium permanganate. Upon discharge, she was free of symptoms. Up to the present time, 19 cases, including our case of BehÇet’s disease with amyloidosis, have been reported. Four cases in Japan have been reported in Japanese. Reviewing the 19 cases, however, all of them developed initially as BehÇet’s disease, followed by amyloidosis and there have only been three cases including ours where the type of amyloid was studied. Our finding was identical to the previous two reports; namely identification of the AA type which is common in secondary amyloidosis. These findings lead to a conclusion that amyloidosis associated with BehÇet’s disease is secondary.
    Journal of Gastroenterology 22(4):487-495. DOI:10.1007/BF02773818 · 4.02 Impact Factor
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    ABSTRACT: Nineteen specimens of normal tissue were obtained from the duodenum, upper jejunum and terminal ileum. Specimens were fixed with periodatelysine-paraformaldehyde and then frozen sections were made. Lymphoid cell subsets were identified by indirect immunoperoxidase staining using mouse anti-human monoclonal antibodies. Results: 1) There was a remarkable variation in the distribution of positive cells among individuals and even within single sections. 2) Lymphoid cell subsets of the three parts of the small intestine were almost identical except for the difference in IgA subclasses of IgA-containing cells. IgAl predominated over IgA2 in the jejunum while IgA2 predominated over IgAl in the ileum. The subsets in the lamina propria (LP) and in the epithelium were clearly distinct; Leu 3 + (helper/inducer T) predominated in LP while Leu 2 + (suppressor/cytotoxic T) predominated in the epithelium and no Ig-containing cells were found in the epithelium. In LP the mean ratio of Leu 1 + (pan T): IgA (IgAl + IgA2) containing cells: IgM: IgG: IgD: IgE: HNK-1 + was 48.9: 36.6: 9.0: 1.8: 1.0: 0.3: 2.4. 3) The staining patterns of HLA-DR + and Leu 10 + were similar; dense in the top in LP of the villi. In some individuals the apical portion of epithelial cells was stained by anti-HLA-DR. 4) A few positive cells were observed in only two out of 19 cases by anti IL-2R.
    Journal of Gastroenterology 21(4):336-343. DOI:10.1007/BF02774130 · 4.02 Impact Factor
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    ABSTRACT: A case of ulcerative colitis (UC) associated with anti-erythrocyte antibodies is reported. A 27-year-old male visited our department because of a relapse of UC, and his condition was diagnosed as severe and extensive colitis. He was vigorously treated with prednisolone (50mg/day i.v.) and TPN, but no response was obtained. Subtotal colectomy with ileostomy was performed on the 22nd hospital day, and a proctosigmoidectomy was performed on him six months later. Preoperative blood type and screen disclosed that his blood was not cross-matched either on the forward or reverse typing. His ABO blood group could not be identified as “B”, as determined upon admission, and his Coombs’ test was positive. Further studies revealed that his Rh group was CcDEe. Anti-I antibody was detected in his serum and eluate from erythrocytes were identified as anti-E and c. Titers of the antibodies were strongest in the preoperative period and became weak following colectomy, but the antibodies did not disappear even after the large bowel was totally removed.
    Journal of Gastroenterology 23(5):564-569. DOI:10.1007/BF02779490 · 4.02 Impact Factor
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    ABSTRACT: A case of acute pancreatitis induced by salicylazosuifapyridine (Salazopyrin, SASP) was reported. A 33-year-old man with ulcerative colitis was given SASP. Five weeks later, P-type serum amylase was found to be elevated. The amylase/creatinine clearance ratio (ACCR) and serum lipase were also elevated. There were neither subjective symptoms nor abnormal ultrasound findings in the pancreas. Lymphocyte stimulation test (LST) to SASP was positive. Asymptomatic pancreatitis by SASP was suspected and SASP administration was halted. Afterwards the abnormal data became normal. Readministration of SASP because of relapse caused an episode of pancreatitis similar to the first occasion. LST was negative before SASP intake and became positive after intake. Desensitization to SASP was unsuccessful. LST was negative before attempting desensitization and became positive when the dosage of SASP increased to 100 mg daily. This is the second case of acute pancreatitis reported to be induced by SASP and this is the first case in which LST to SASP was described. To our knowledge, this is also the first case in which a positive LST was described in drug-induced pancreatitis. This case provides evidence for the role of delayed type hypersensitivity in the etiopathogenesis of SASP allergy and of dose-independent drug-induced pancreatitis.
    Journal of Gastroenterology 22(2):228-233. DOI:10.1007/BF02774222 · 4.02 Impact Factor
  • Gastroenterologia Japonica 20(3).
  • Gastroenterologia Japonica 21(2). DOI:10.1007/BF02774836
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    ABSTRACT: Class II antigen expression on gastric epithelium was investigated using an immunoperoxidase method in relation to the degree of inflammatory cell infiltration in the lamina propria. Sixty-six biopsy specimens from 43 patients with chronic gastritis were examined. The frequency of HLA-DR expression in specimens with cell infiltration was 94%, while that in specimens without cell infiltration was 24%. There was significant difference in the frequency of HLA-DR expression between the two groups (P<0.01). HLA DR fwas most intensely expressed in the glandular neck portion. The frequency and extent of class II antigen expression on gastric epithelium with cell infiltration were in the following order: DR>DP>DQ. The extent of DR and DP, but not DQ expression generally paralleled the degree of cell infiltration. Intestinal metaplasia was found in 13 specimens. In the area of intestinal metaplasia, epithelial class II staining was absent except for one specimen. These results suggest that the respectivegenes of three class II antigens are regulated by different mechanisms and that an immunological mechanism plays a role in the pathogenesis of gastritis.
    Journal of Gastroenterology 27(1):23-28. DOI:10.1007/BF02775060 · 4.02 Impact Factor
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    ABSTRACT: Although the expression of HLA-DR antigens on colonic epithelium in ulcerative colitis has been observed by several groups, the results of the expression in remission have been conflicting and there has been virtually no study concerning the expression in non-inflamed area in active ulcerative colitis. We studied systematically HLA-DR expression on colonic epithelium in 37 patients with ulcerative colitis chronologically, namely in remission as well as in the active stage and inflamed and non-inflamed areas simultaneously in the active stage. HLA-DR antigens were detected by indirect peroxidase staining using anti-HLA-DR monoclonal antibody. We confirmed the previous observation that epithelium from control colon does not express HLA-DR antigens, while epithelium from ulcerative colitis expresses the antigens with high frequency (83.3 percent). In addition, we demonstrated that HLA-DR expression on colonic epithelium in active ulcerative colitis disappeared in remission. Our new finding was that there is no HLA-DR expression on colonie epithelium in non-inflamed mucosa in active ulcerative colitis. Namely HLA-DR antigens were expressed only on inflamed epithelium of ulcerative colitis. These results lead to the conclusion that the expression of HLA-DR antigens on colonic epithelium in ulcerative colitis is closely related to the inflammation of mucosa.
    Journal of Gastroenterology 22(5):571-577. DOI:10.1007/BF02776716 · 4.02 Impact Factor

Publication Stats

41 Citations
82.58 Total Impact Points

Institutions

  • 2012
    • Akita General Hospital
      Akita, Akita, Japan
  • 1998–2008
    • Akita University Hospital
      Akita, Akita, Japan
  • 1990
    • Akita University
      • First Department of Internal Medicine
      Akita, Akita-ken, Japan