Gloria Dávila-Ortiz

National Polytechnic Institute, Ciudad de México, Mexico City, Mexico

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Publications (33)54.45 Total impact

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    ABSTRACT: Jatropha curcas is an oil seed plant that belongs to the Euphorbiaceae family. Nontoxic genotypes have been reported in Mexico. The purpose of the present work was to evaluate the effect of a Mexican variety of J. curcas protein concentrate (JCP) on weight gain, biochemical parameters, and the expression of genes and proteins involved in insulin signaling, lipogenesis, cholesterol and protein synthesis in rats. The results demonstrated that short-term consumption of JCP increased serum glucose, insulin, triglycerides and cholesterol levels as well as the expression of transcription factors involved in lipogenesis and cholesterol synthesis (SREBP-1 and LXRα). Moreover, there was an increase in insulin signaling mediated by Akt phosphorylation and mTOR. JCP also increased PKCα protein abundance and the activation of downstream signaling pathway targets such as the AP1 and NF-κB transcription factors typically activated by phorbol esters. These results suggested that phorbol esters are present in JCP, and that they could be involved in the activation of PKC which may be responsible for the high insulin secretion and consequently the activation of insulin-dependent pathways. Our data suggest that this Mexican Jatropha variety contains toxic compounds that produce negative metabolic effects which require caution when using in the applications of Jatropha-based products in medicine and nutrition.
    Plant Foods for Human Nutrition 08/2015; DOI:10.1007/s11130-015-0502-9 · 2.42 Impact Factor
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    ABSTRACT: The role of the peptides-phenolic compounds (PC) interaction on the antioxidant capacity profile (ACP) of protein hydrolysates from rapeseed (Brassica napus) was studied in 36 hydrolysates obtained from a PC-rich and PC-reduced protein substrate. The latent profile analysis (LPA), with data of seven in vitro methods and one assay for cellular antioxidant activity (CAA), allowed identifying five distinctive groups of hydrolysates, each one with distinctive ACP. The interaction of peptides with naturally present PC diminished in vitro antioxidant activity in comparison with their PC-reduced counterparts. However, CAA increased when peptides-PC interaction occurred. The profile with the highest average CAA (62.41±1.48%), shown by hydrolysates obtained by using alcalase, shared typical values of Cu(2+)-catalysed β-carotene oxidation (62.41±0.43%), β-carotene bleaching inhibition (91.75±0.22%) and Cu(2+)-chelating activity (74.53±0.58%). The possibilities for a sample to exhibit ACP with higher CAA increased with each unit of positively charged amino acids, according to multinomial logistic regression analysis. Copyright © 2014 Elsevier Ltd. All rights reserved.
    Food Chemistry 07/2015; 178:346-57. DOI:10.1016/j.foodchem.2014.12.063 · 3.39 Impact Factor
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    ABSTRACT: The involvement of angiotensin-I-converting enzyme (ACE-I) as one of the mechanisms controlling blood pressure is being studied to find alternative means of control of hypertension on human beings. On the market there are synthetic drugs that can control it, but these can cause undesirable health side effects. In this work was assessed the fractionation by ultrafiltration of the Lima bean (Phaseolus lunatus) and Jamapa bean (Phaseolus vulgaris), protein hydrolysates obtained with Alcalase(®) and Flavourzyme(®) on ACE-I inhibitory activity. Four membranes of different molecular cutoffs (10, 5, 3, and 1 kDa) were used. Fractions that had a higher inhibitory activity in both legumes were denominated as E (<1 kDa) with IC50 of 30.3 and 51.8 μg/mL values for the P. lunatus with Alcalase and Flavourzyme, respectively, and for the Phaseolus vulgaris with Alcalase and Flavourzyme with about 63.8 and 65.8 μg/mL values, respectively. The amino acid composition of these fractions showed residues in essential amino acids, which make a good source of energy and amino acids. On the other hand, the presence of hydrophobic amino acids such as V and P is a determining factor in the ACE-I inhibitor effect. The results suggest the possibility of obtaining and utilizing these peptide fractions in the development and innovation of a functional product that helps with treatment and/or prevention of hypertension.
    Journal of medicinal food 05/2015; DOI:10.1089/jmf.2015.0007 · 1.70 Impact Factor
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    ABSTRACT: Phaseolus lunatus protein concentrates and the proteases Alcalase(R) and Pepsin-Pancreatin were used for the production of protein hydrolysates that inhibit angiotensin-I converting enzyme (ACE). Protein concentrate obtained from germinated and ungerminated seeds flour was hydrolyzed with Alcalase(R) at enzyme/substrate ratio (E/S) 1/10 and during 0.5 and 2.0 h, respectively. On the other hand, protein concentrate obtained from ungerminated (E/S: 1/10) and germinated (E/S: 1/50) seeds flour was sequentially hydrolyzed with Pepsin-Pancreatin during 1.0 and 3.0 h, respectively. Peptide fractions with ACE inhibitory activity in a range of 0.9 to 3.8 µg/mL were obtained by G-50 gel filtration chromatography and high- performance liquid chromatography C18 reverse phase chromatography. The observed amino acid composition suggests a substantial contribution of hydrophobic residues to the peptides’ inhibitory potency, which potentially acts via blocking of angiotensin II production. These results show that P. lunatus seed proteins are a potential source of ACE inhibitory peptides when hydrolyzed with Alcalase(R) and Pepsin-Pancreatin.
    03/2015; 35(1):167-174. DOI:10.1590/1678-457X.6551
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    ABSTRACT: Legumes in combination with other products are the staple food for a large part of the world population, especially the low-income fragment, because their seeds provide valuable amounts of carbohydrates, fiber, and proteins, and have an important composition of essential amino acids, the sulphured amino acids being the limiting ones. Furthermore, legumes also have nonnutritional compounds that may decrease the absorption of nutrients or produce toxic effects; however, it has been reported that depending on the dose, these nonnutritional compounds also have different bioactivities as antioxidant, hypolipidemic, hypoglycemic, and anticarcinogenic agents, which have been proven in scientific studies. It has been observed that in countries with a high consumption of legumes, the incidence of colorectal cancer is lower. Some studies have shown that legume seeds are an alternative chemopreventive therapy against various cancers especially colon; this was verified in various animal models of induced by azoxymethane, a colon specific carcinogenic compound, in which a diet was supplemented with different concentrations of beans, lentils, chickpeas, or soybeans, mostly. These studies have proven the anticancer activity of legumes in early stages of carcinogenesis. Therefore, it is important to review the information available to elucidate the chemopreventive mechanisms of action of legume compounds.
    Nutrition and Cancer 02/2015; 67(3):1-10. DOI:10.1080/01635581.2015.1004729 · 2.47 Impact Factor
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    ABSTRACT: Significant initiatives exist within the global food market to search for new, alternative protein sources with better technological, functional, and nutritional properties. Lima bean (Phaseolus lunatus L.) protein isolate was hydrolyzed using a sequential pepsin-pancreatin enzymatic system. Hydrolysis was performed to produce limited (LH) and extensive hydrolysate (EH), each with different degrees of hydrolysis (DH). The effects of hydrolysis were evaluated in vitro in both hydrolysates based on structural, functional and bioactive properties. Structural properties analyzed by electrophoretic profile indicated that LH showed residual structures very similar to protein isolate (PI), although composed of mixtures of polypeptides that increased hydrophobic surface and denaturation temperature. Functionality of LH was associated with amino acid composition and hydrophobic/hydrophilic balance, which increased solubility at values close to the isoelectric point. Foaming and emulsifying activity index values were also higher than those of PI. EH showed a structure composed of mixtures of polypeptides and peptides of low molecular weight, whose intrinsic hydrophobicity and amino acid profile values were associated with antioxidant capacity, as well as inhibiting angiotensin-converting enzyme. The results obtained indicated the potential of Phaseolus lunatus hydrolysates to be incorporated into foods to improve techno-functional properties and impart bioactive properties.
    07/2014; 34(3):441-448. DOI:10.1590/1678-457x.6349
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    ABSTRACT: BACKGROUND: Bean seeds are an inexpensive source of protein. Anthracnose disease caused by the fungus Colletotrichum lindemuthianum results in serious losses in common bean (Phaseolus vulgaris L.) crops worldwide, affecting any above-ground plant part, and protein dysfunction, inducing the synthesis of proteins that allow plants to improve their stress tolerance. The aim of this study was to evaluate the use of beans damaged by anthracnose disease as a source of peptides with angiotensin-converting enzyme (ACE-I)-inhibitory activity. RESULTS: Protein concentrates from beans spoiled by anthracnose disease and from regular beans as controls were prepared by alkaline extraction and precipitation at isolelectric pH and hydrolysed using Alcalase 2.4 L. The hydrolysates from spoiled beans had ACE-I-inhibitory activity (IC(50) 0.0191 mg protein mL(-1) ) and were very similar to those from control beans in terms of ACE-I inhibition, peptide electrophoretic profile and kinetics of hydrolysis. Thus preparation of hydrolysates using beans affected by anthracnose disease would allow for revalorisation of this otherwise wasted product. CONCLUSION: The present results suggest the use of spoiled bean seeds, e.g. anthracnose-damaged beans, as an alternative for the isolation of ACE-I-inhibitory peptides to be further introduced as active ingredients in functional foods. Copyright © 2012 Society of Chemical Industry.
    Journal of the Science of Food and Agriculture 03/2013; 93(4). DOI:10.1002/jsfa.5841 · 1.88 Impact Factor
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    ABSTRACT: Jatropha curcas seed cake is a protein-rich byproduct of oil extraction which could be used to produce protein isolates. The purpose of this study was the optimization of the protein isolation process from the seed cake of an edible provenance of J. curcas by an alkaline extraction followed by isoelectric precipitation method via a sequentially integrated optimization approach. The influence of four different factors (solubilization pH, extraction temperature, NaCl addition, and precipitation pH) on the protein and antinutritional compounds content of the isolate was evaluated. The estimated optimal conditions were an extraction temperature of 20°C, a precipitation pH of 4, and an amount of NaCl in the extraction solution of 0.6 M for a predicted protein content of 93.3%. Under these conditions, it was possible to obtain experimentally a protein isolate with 93.21% of proteins, 316.5 mg 100 g−1 of total phenolics, 2891.84 mg 100 g−1 of phytates and 168 mg 100 g−1 of saponins. The protein content of the this isolate was higher than the content reported by other authors.
    02/2013; 2013. DOI:10.5402/2013/197201
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    ABSTRACT: Bean protein isolate and phaseolin were hydrolysed using pepsin and pancreatin, and the resulting hydrolysates were filtered through a 1kDa cut-off membrane and fractionated by size exclusion chromatography. Three fractions corresponding to MW 0.7-1.0kDa, 0.43-0.7kDa and <0.43kDa (A1, A2, and A3 for protein isolate fractions, and B1, B2, and B3 for phaseolin fractions) were assayed for antioxidant and metal chelating activity and they were also subjected to amino acid and SDS-PAGE analysis. Fractions A1 and B1 had the highest copper chelating activity (78% and 82%, respectively), while iron chelating activity was the highest in fractions A1 and B3 (36% and 16%, respectively). Fractions A2 and B3 had the highest antioxidant activity as determined by inhibition of reducing power and β-carotene bleaching, while the highest ABTS radical scavenging activity was found in A3 and B3. Thus, fractions coming from the isolate and phaseolin had similar activities except for iron chelation, suggesting that phaseolin is the major contributor to the antioxidant and copper chelating activities of the hydrolysed protein isolate.
    Food Chemistry 12/2012; 135(3):1789-95. DOI:10.1016/j.foodchem.2012.06.016 · 3.39 Impact Factor
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    ABSTRACT: Proteomic and peptidomic studies are emerging as an important part of the holistic approach to food science and technology and have been recently applied in the study and production of bioactive peptides. Food-derived bioactive peptides are short amino acid chains with a known sequence that may have one or more biological activities. The proteomic and peptidomic approach to bioactive peptide studies includes bioinformatics, chemometric tools and proteomic/peptidomic methods. A proteomic and peptidomic approach applied to the study of bioactive peptides allows optimizing their production and finding peptides of interest and contributes to understand the interaction mechanisms between receptor and bioactive peptides. The objective of this review was to describe recent analytical tools used for studying various aspects of food-derived biopeptides, emphasizing their production at laboratory and industrial scale.
    Food Engineering Reviews 12/2012; 4(4). DOI:10.1007/s12393-012-9058-8 · 3.04 Impact Factor
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    ABSTRACT: The pyrimidine glycosides, vicine and convicine, are compounds of the genus Vicia, whereas their aglycone derivatives, divicine and isouramil, are responsible for favism occurrence, and L-3,4-dihydroxyphenylalanine (L-DOPA) is a nonprotein amino acid precursor of dopamine in healthy neurons. The present work evaluated the effect of thermal treatment, which consisted of roasting and boiling, on the content of vicine, convicine and L-DOPA in mature cotyledons of 10 varieties of Mexican Vicia faba L. and determined the concentration of glycosides and L-DOPA by high-performance liquid chromatography. The raw cotyledons showed 2.886.10, 0.631.68 and 0.280.44 vicine, convicine and L-DOPA, respectively. The 12 and 40% and 30 and 61% decrease in vicine and convicine content, respectively, were observed to be the effect of roasting and boiling the samples. L-DOPA was totally removed by both thermal processes. In all analyzed samples, vicine was the main compound; however, there were significant differences between varieties and thermal treatments. Practical Applications Vicine and convicine glycosides are strongly implicated in favism, a hemolytic anemia in humans, and in poor performance of laying hens. There are reports of decreased egg weight, increase in the fragility of the yolk and a number of blood stains, and decreased fertility and hatchability of eggs. The characterization of new varieties of faba bean in their content of potentially toxic glycosides allows selection of those with the lowest possible content of such compounds. Also, the application of heat treatments that reduce glycosides will allow the use of seeds or flour to develop harmless food and feed ingredients.
    Journal of Food Quality 09/2012; DOI:10.1111/jfq.12006 · 0.77 Impact Factor
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    ABSTRACT: A 23 factorial design with four replicates of the central trial was used to evaluate wet-fractionation conditions for complete use of hard-to-cook (Phaseolus vulgaris L.) seeds. Tested variables were flour/water ratio (1:5, 1:7.5 and 1:10 w/v), pH (8, 9.5 and 11) and a soaking time (1, 2 and 3 h). The optimum conditions (1:10 w/v flour/water ratio; pH 8; and 1 h soaking time) were determined by the percentages of protein and starch recovery. Three fractions were obtained by the process: a protein isolate, starch fraction and fibrous residue. Isolate crude protein content was 73.03%; its in vitro digestibility was 76.7%, and its amino acids concentrations, except for methionine and cysteine, met essential amino acids requirements for preschool children and adults. Total starch content was 71.41% with an amylose content of 21.46% and an amylopectin content of 78.54%. Total dietary fibre in the fibrous residue was 42.12%, with 81.10% insoluble fibre and 18.91% soluble fibre. The protein isolate’s high protein content, the purity of the starch fraction and high proportion of insoluble fibre in the fibrous residues show wet fractionated hard-to-cook bean to be a promising source of functional ingredients with possible food industry applications.
    Food and Bioprocess Technology 07/2012; 5(5). DOI:10.1007/s11947-010-0451-0 · 3.13 Impact Factor
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    ABSTRACT: A search in a database of potential bioactive short sequences in food proteins reveals that bioactive peptides with a variety of beneficial effects for cardiovascular health are present in the sequence of common bean proteins, including bioactive sequences with antioxidant properties. A protein isolate, the storage protein phaseolin and a lectin extract from Phaseolus vulgaris L. var. Jamapa, were hydrolyzed by treatment with pepsin and pancreatin in order to investigate the possible release of peptides with antioxidant and metal chelating properties. Antioxidant activity was determined in Caco-2 cells exposed to a free radical generator, and iron and copper chelating activities were determined using colorimetric methods. The highest antioxidant activity, 71% inhibition, was found in the hydrolyzed protein isolate. Copper and iron chelating activities were highest in the lectin and phaseolin hydrolysates, 53% and 81%, respectively. Thus, experimental data indicates, as suggested by the database search, that antioxidant peptides are abundant in pepsin–pancreatin hydrolysates, which may represent a valuable health-promoting property in common bean.
    Food Chemistry 04/2012; 131(4). DOI:10.1016/j.foodchem.2011.09.084 · 3.39 Impact Factor
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    ABSTRACT: Protein concentrates from ungerminated and germinated lima bean Phaseolus lunatus seed flours were hydrolyzed with alcalase 2.4L or pepsin-pancreatin sequential hydrolysis, and ACE inhibitory activity measured in the different hydrolysis treatments. A 2 3 factorial design with four replicates of the central treatment was used. Evaluated factors were protein concentrate source (ungerminated seeds, PC 1 ; germinated seeds, PC 2), enzyme/substrate ratio E/S (1/50 or 1/10) and hydrolysis time (0.5 or 2.0 h for alcalase; 1 or 3 h for pepsin-pancreatin). Degree of hydrolysis (DH) was high for the alcalase hydrolysates (24.12-58.94%), but the pepsin-pancreatin hydrolysates exhibited the highest ACE inhibitory activity (IC 50 = 0.250-0.692 mg/mL). Under the tested conditions, the hydrolysates with the highest ACE inhibitory activity were produced with sequential pepsin-pancreatin using either PC 1 at 1 h hydrolysis time and a 1/10 E/S ratio or PC 2 at 1 h hydrolysis time and a 1/50 E/S ratio. Lima bean protein hydrolysates * Telephone (52) 999 9460989, Fax (52) 999 9460994.
    ANGIOTENSIN-NEW RESEARCH, Edited by Sōta Harada and Itsuki Moi, 03/2012: pages 18; Nova Science Publishers., ISBN: 978-1-62100-773-9
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    ABSTRACT: J. curcas seed proteins were fractioned according to the Osborne method and some biochemical properties were determined for these fractions. Glutelins (378 g kg(-1) protein) and globulins (201 g kg(-1) protein) were the main components. Albumins and prolamins were the minor components. Protein digestibility was highest in glutelins and globulins with values of 81 and 80% respectively. Electrophoresis analysis showed that globulins and glutelins exhibited similar polypeptide profiles. Electrophoresis patterns suggested that there could be a structural relationship among 2S, 7S and 11S storage proteins from plant sources. According to the FAO/WHO reference, the protein fractions had acceptable levels of most of the essential amino acids, but its globulins and glutelins were low in lysine and tryptophan.
    Grasas y Aceites 03/2012; 63(3):253-259. DOI:10.3989/gya.072511 · 1.08 Impact Factor
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    ABSTRACT: Several protein sources can be used to produce bioactive peptides with angiotensin I-converting enzyme (ACE) inhibit-tory activity. Protein concentrates from ungerminated and germinated lima bean Phaseolus lunatus seed flours were hydrolyzed with Alcalase 2.4 L or pepsin-pancreatin sequential hydrolysis, and ACE inhibitory activity measured in the different hydrolysis treatments. Protein hydrolysate production was analyzed with a 2 3 factorial design with four repli-cates of the central treatment. Evaluated factors were protein concentrate source (ungerminated seeds, PC 1 ; germinated seeds, PC 2), enzyme/substrate ratio E/S (1/50 or 1/10) and hydrolysis time (0.5 or 2.0 h for Alcalase; 1 or 3 h for pep-sin-pancreatin). Degree of hydrolysis (DH) was high for the Alcalase hydrolysates (24.12% -58.94%), but the pep-sin-pancreatin hydrolysates exhibited the highest ACE inhibitory activity (IC 50 = 0.250 -0.692 mg/mL). Under the tested conditions, the hydrolysates with the highest ACE inhibitory activity were produced with sequential pep-sin-pancreatin using either PC 1 at 1 h hydrolysis time and a 1/10 E/S ratio or PC 2 at 1 h hydrolysis time and a 1/50 E/S ratio. Lima bean protein hydrolysates prepared with Alcalase or pepsin-pancreatin are a potential ingredient in the pro-duction of physiologically functional foods with antihypertensive activity.
    Food and Nutrition Sciences 01/2012; 03(04). DOI:10.4236/fns.2012.34072
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    ABSTRACT: The changes in SDS-PAGE proteins patterns, oligosaccharides and phenolic compounds of L. campestris seeds, were evaluated during nine germination days. SDS-PAGE pattern showed 12 bands in the original protein seeds, while in the samples after 1–9 germination days, the proteins located in the range of 28–49 and 49–80 kDa indicated an important reduction, and there was an increase in bands about 27 kDa. On the other hand, oligosaccharides showed more than 50% of decrease in its total concentration after 4 germination days; nevertheless after the fifth day, the oligosaccharides concentration increases and rises more than 30% of the original concentration. Phenolic compounds increased their concentration since the first germination day reaching until 450% more than the original seed level. The obtained results are related with liberation or increase of phenolic compounds with antioxidant properties, allowing us to suggest that the germination would be used to produce legume foods for human consumption with better nutraceutical properties.
    International Journal of Agronomy 01/2012; 2012. DOI:10.1155/2012/387407
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    Hispanic Foods: Chemistry and Bioactive Compounds, 01/2012: chapter MICROSTRUCTURE OF MATURE GREEN MEXICAN VANILLA PODS Vanilla planifolia (Andrews) BY MICROSCOPY TECHNIQUES AND DIGITAL IMAGE ANALYSIS; ACS Books.
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    ABSTRACT: In this work, microstructural changes of the tissues of vanilla were evaluated during the curing, using an Environmental Scanning Electron Microscope (ESEM). The morphometric parameters: area (A), perimeter (P), shape factor (SF) and compactness (C) of each tissue (epicarp, mesocarp and vascular bundle) were quanti�ed by Digital Image Analysis (DIA). Results indicated that curing induces structural disruptions of the vanilla tissues which is more pronounced in the mesocarp. Shape factor and compactness showed highest values in pods subjected to 10 cycles of sunning-sweating (10 SS) in which the highest concentration of vanillin and the lowest water looses were detected. It is possible to recommend a reduction of curing time from 20-25 SS cycles to 10.
    Revista mexicana de ingeniería química 01/2011; 10(1):105-115. · 0.95 Impact Factor
  • Journal of Biotechnology 11/2010; 150:309-310. DOI:10.1016/j.jbiotec.2010.09.283 · 2.88 Impact Factor

Publication Stats

260 Citations
54.45 Total Impact Points

Institutions

  • 2002–2015
    • National Polytechnic Institute
      • Departamento de Graduados en Alimentos
      Ciudad de México, Mexico City, Mexico
  • 2005
    • National University of La Plata
      Eva Perón, Buenos Aires, Argentina
    • Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria
      Madrid, Madrid, Spain