Nan-sheng Wan

Publications (2)0 Total impact

• Article: [Impact of chronic intermittent hypoxia upon rat liver lipid metabolism and interventional effect of Tempol].

  Hai-Yan Zhao, Bao-Yuan Chen, Run Guo, Cheng-Ying Kong, Nan-Sheng Wan, Jing Zhang
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  ABSTRACT: To explore the impact of chronic intermittent hypoxia (CIH) upon rat liver lipid metabolism and effect of anti-oxidant Tempol. Male Wistar rats (n = 80) were randomly divided into intermittent hypoxia group (10, 20, 30, 40 times/h), intermittent hypoxia Tempol treatment group, intermittent hypoxia normal saline treatment group, intermittent air mimic group (IA) and blank control group (CG). Sections of liver were stained with hematoxylin and eosin. Serum levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were measured. Levels of liver homogenate triglyceride (TG), total cholesterol (TC), free fatty acids (FFA) and serum TG, TC, adiponectin (ADP) were measured. Liver histology: IH group exhibited hepatocellular swelling, hyperchromatosis, disrupted hepatocellular membrane. With the increase of frequency, there were local necrosis and infiltration of inflammatory cells. But no steatosis was seen. Tempol early treatment and IA groups exhibited no hepatocellular swelling or inflammatory cell infiltration. The activities of ALT and AST increased along with the increased frequency in IH group (all P < 0.01). The levels of ALT and AST in IH group ((48.6 ± 3.6), (25.4 ± 2.6) U/L) were higher than those in IA group ((20.3 ± 3.1), (18.7 ± 1.3) U/L) and CG group ((17.5 ± 2.4), (18.8 ± 1.3) U/L) (all P < 0.01). It decreased in Tempol treatment group, and more obviously when early intervention was applied (all P < 0.01). Liver homogenate TG, TC and FFA had no difference among IH, IA and CG groups (all P > 0.05), and no difference in different frequencies in IH group (all P > 0.05). The levels of serum TG, TC in IH groups were higher than those in IA and CG groups while ADP was lower (all P < 0.01). It changed more obviously in different frequencies in IH group (all P < 0.01). In Tempol treatment group, serum TG, TC decreased while ADP increased and changed more obviously when early intervention was applied (all P < 0.01). CIH causes the morphologic changes of liver and the elevations of ALT and AST, but results not in lipid deposition in liver cells. Anti-oxidation of Tempol can block intermittent hypoxia associated with liver injury.
  Zhonghua yi xue za zhi 02/2013; 93(6):407-10.
• Article: [The effects of chronic intermittent hypoxia on blood pressure and sympathetic nerve activity in rats].

  Nan-sheng Wan, Bao-yuan Chen, Jing Feng, Shuo Li, Wei Zhou, Zhen Zhang, Run Guo
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  ABSTRACT: To observe the changes of blood pressure and sympathetic nerve activity under different degrees of chronic intermittent hypoxia (CIH) in rats, and therefore to explore the effects of CIH on blood pressure and sympathetic nerve activity and the correlation between blood pressure and sympathetic nerve activity in the pathogenesis of CIH-induced hypertension. Male Wistar rats (n = 168) were randomly divided into untreated group (UD), severe intermittent hypoxia group (IH(1)), moderate intermittent hypoxia group (IH(2)), mild intermittent hypoxia group (IH(3)), continuous hypoxia group (CH), sham control group (SC) and were exposed to different conditions. Rats (n = 8) of the UD group were sacrificed before the experiment, while rats of the other groups were killed in weeks 2, 4, 6, and 8 of the experiment. Anticoagulated venous blood was obtained and plasma was stored at -80°C. Systolic arterial pressure (SBP) was recorded before and after the experiment, while plasma norepinephrine (NE) was measured after the experiment. Before the experiment, the SBP of rats showed no significant differences among groups (F = 0.008, P > 0.05), but the SBP of rats in the intermittent hypoxia groups increased gradually, and higher than the UD group, the SC group and the CH group from the beginning of week 4 (P < 0.05 or P < 0.01). The blood pressure was positively related with the degree of hypoxia (F = 9.844, P < 0.01), and that of the IH(1) group was significantly higher than that of the IH(3) group (P < 0.05), while no significant changes were found in the SC and the CH groups. The plasma NE level of rats in the intermittent hypoxia groups increased gradually with the experiment and significantly higher than that of the UD group, the SC group and the CH group at week 8 (P < 0.05 or P < 0.01), and the level of NE was positively related with the degree of hypoxia (F = 11.537, P < 0.01). The NE level of the IH(1) group was significantly higher than that of the IH(3) group (P < 0.05), but no significant change was found in the SC and the CH groups. The plasma NE levels were positively related with blood pressure (r = 0.530, P < 0.01). CIH can cause increased blood pressure and sympathetic activity in rats, and the effect was dependent on the degree of hypoxia and the time of exposure. The results suggested that CIH-induced higher blood pressure was associated with increased sympathetic activity.
  Zhonghua jie he he hu xi za zhi = Zhonghua jiehe he huxi zazhi = Chinese journal of tuberculosis and respiratory diseases 01/2012; 35(1):29-32.