ABSTRACT: The influence of medium pH conditions on regulation of the sal operon was quantitatively measured by using a genetically engineered Acinetobacter baylyi ADP1 strain ADPWH_lux. Live-cell based bioluminescence assays determined that the SalR, a LysR-type transcriptional regulator (LTTR), functioned in a range of medium pH conditions. During exponential growth, SalR regulated bioluminescence production peaked at the neutral pH and tolerated medium pH changes of 5.5-9.0. In the stationary phase, the SalR regulated gene expression reached 20-35% of the maximum productivity during the exponential phase. This report reveals the influence of pH conditions on a LTTR system and demonstrates that the biosensor strain can be a useful tool for understanding transcriptional regulations in different environments.
Journal of microbiological methods 08/2009; 79(1):8-12. · 2.43 Impact Factor