John M. Peltier,
Srdjan Askovic,
Robert R. Becklin,
Cindy Lou Chepanoske,
Yew-Seng J. Ho,
Vladimir Kery, Shuping Lai,
Tahmina Mujtaba,
Mike Pyne,
Paul B. Robbins,
Moritz von Rechenberg,
Bonnie Richardson,
Justin Savage,
Peter Sheffield,
Sam Thompson,
Lawrence Weir,
Kartika Widjaja,
Nafei Xu,
Yuejun Zhen,
J. Jay Boniface
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ABSTRACT: Proteomics-based technologies have the potential to accelerate the development of drugs, but such technologies must be well integrated in order to have a positive impact. We describe, herein, a multi-step process for the discovery of protein–protein interactions. It is shown that process stages are interdependent and can influence, either positively or negatively, subsequent steps. Optimization of each step, in the context of the full process, is essential for the overall success of the experiment.
International Journal of Mass Spectrometry.
