Publications (2)1.5 Total impact
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Article: Human oral epithelial cell culture I. Improved conditions for reproducible culture in serum-free medium
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ABSTRACT: Gingival tissue from healthy adult human donors was used as a source of epithelial cells for culture. An overnight incubation of this tissue with dispase facilitated the mechanical separation of the surface epithelium from the underlying fibrous connective tissue. This step minimized culture contamination with fibroblasts. The epithelium was then trypsinized to prepare a single cell suspension. The cell pellets were collected by centrifugation and resuspended in keratinocyte growth medium, incubated at 37° C and 5% CO2 in a humid atmosphere. Primary cultures grew in small islands that coalesced at confluency. Immunohistochemistry demonstrated uniform staining of the cells with antibodies to keratins of stratified squamous epithelium. Ultrastructurally, the cells contained distinct intermediate filaments. When cells were grown in media with low calcium (0.15 mM), cell-to-cell contacts were via interlacing papillary projections with no desmosomes. However, when cells were grown under physiologic calcium (1.2 mM), desmosomes were prominent and well developed. Cells were maintained in culture for over 100 d (7 passages).In Vitro Cellular & Developmental Biology - Plant 05/1990; 26(6):589-595. · 1.50 Impact Factor -
Article: Mechanisms of non-electrolyte penetration across dog and rabbit oral mucosa in vitro
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ABSTRACT: Permeability coefficients were determined for 20 14C-labelled compounds using canine and rabbit lingual frenulum as the test tissue. The oil-to-water distribution coefficients of these compounds differed by over 5 × 105-fold. A linear relationship between permeability coefficients and oil-to-water distribution coefficients was found for those compounds with oil-to-water distribution coefficient greater than that of water, demonstrating the importance of lipid solubility on the rate of transfer across oral mucosa. The data further indicate that hydrogen bonding, cylindrical radius and chemical differences contribute to penetration rates. The plot of permeability coefficient versus molecular volume for compounds with oil-to-water distribution coefficients less than that of water is biphasic, suggesting two different routes of permeation for these compounds. This indicates that oil-soluble compounds traverse oral mucosa via solvation, whereas water-soluble molecules with a molecular volume of less than 80 cm3/mol cross primarily through membrane pores; larger water-soluble molecules pass extracellularly.Archives of Oral Biology.
