ABSTRACT: The use of tobacco, betel nut, betel leaf (pan) and chillies cause certain changes in the oral cavity because of different chemicals they contain. A total of twenty five patients were examined, both male and females,ranging between 10-70 years. Oral examination was carried out and most of them presented with, submucus fibrosis or blanched mucosa. Samples from oral cavity were collected with sterile swabs, swabbing it all round in the oral cavity and carried to the laboratory for both aerobic and anaerobic in Robertson’scooked meat medium(RCM) for further processing. The growth in the culture medium was specifically looked for the normal microbial flora of oral cavity i.e Streptococcus viridans, Coagulase NegativeStaphyloccus, Diptheroides, Neisseria catarrhalis (aerobic flora) and the anaerobic flora. In twenty five patients most of them had no normal microbial flora except five patients who showed presence of 4 to 5 colonies i.e scanty growth of Streptococcus viridans indicating that the normal microbial flora was reduced considerably in these patients. Such patients are more prone to develop precancerous conditions and ultimately the carcinoma of the oral cavity with hundred percent reduction in the normal microbial flora.
Pravara Medical Review. 01/2009;