ABSTRACT: To establish a method for induced and amplified porcine monocyte-derived immature dendritic cells(imDCs).The aim of this study was to assess the pCTLA4-Ig gene-modified porcine imDCs could benefit for prevent the proliferation of xenogeneic spleen cells.
Poricne peripheral blood mononuclear cells(PBMC) were cultured with rpGM- CSF(30 microg/L) and rpIL-4(20 microg/L). To evaluate the suspending cells by transmission electronic microscope and flow cytometer. Porcine imDCs were transduced with adenovirus mediated pCTLA4-Ig gene. The mixed lymphocyte reaction(MLR) was used to examine the proliferation of spleen cells of SD rat to gene-modified imDCs.
With rpGM-CSF and rpIL-4 induced porcine PBMC for 4-5 days, it was exhibited typical morphological characteristics and immunological phenotype of imDCs with high expression of SLA-DR, CD172a and low expression of CD80/CD86 on the cellular surface. The gene-modified imDCs were able to amplified the pCTLA4-Ig and IDO gene by RT-PCR. The stimulate index of gene-modified imDCs group were markedly inferior to that in unmodified imDCs group(P<0.05).
The pCTLA4-Ig gene-modified imDCs can significantly inhibit the proliferation of xenogeneic spleen cells, and there might was correlated with the expression of IDO reduce the immune response of xenogeneic antigen.
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology 05/2010; 26(5):434-7.