T Kameyama

Fukuoka Women's University, Hukuoka, Fukuoka, Japan

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Publications (56)104.58 Total impact

  • [Show abstract] [Hide abstract]
    ABSTRACT: Herpes simplex virus (HSV) is assumed to cause recrudescent lesions, usually through endogenous recurrence and rarely through exogenous re-infection. The occurrence of exogenous re-infection in genital and corneal HSV infections has been previously demonstrated using genomic analysis, while exogenous re-infection in oral-facial HSV infections has not been shown. To confirm the occurrence of exogenous HSV re-infection in oral-facial HSV infections. Seven isolates (isolates 1-7) of herpes simplex virus type 1 (HSV-1) were sequentially separated from the same individual. Genomic profiles of HSV-1 isolates were studied: (i) by analysis of 20 RFLPs (restriction fragment length polymorphisms) and (ii) by the determination of nucleotide sequences of a PCR-amplified DNA fragment encompassing reiteration VII (hypervariable region) that belongs to sequences containing short tandem repeats. Isolates 1-5 were the same (F83 genotype) and isolates 6 and 7 were the same (F84 genotype), although isolates 1-5 were markedly different from isolates 6 and 7 in genomic profiles. The infection associated with isolates 6 and 7 was due to exogenous re-infection with F84 genotype virus, thus indicating the occurrence of exogenous HSV re-infection in oral-facial HSV infections.
    Journal of Clinical Virology 09/2007; 39(4):266-70. · 3.29 Impact Factor
  • Kenichi Umene, Chihiro Koga, Tadamitsu Kameyama
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    ABSTRACT: The restriction fragment length polymorphism (RFLP) was analyzed using a set of herpes simplex virus type 1 (HSV-1) strains isolated from oro-facial lesions (oro-facial site collection), which was composed of two subsets: one subset consisted of 57 strains from primary oro-facial lesions, and the other of 47 strains from recurrent oro-facial lesions of patients complicated by factors possibly triggering the recurrence (e.g. malignancy, operation, and treatment of dental caries). RFLP analysis was carried out previously on two other sets of HSV-1 strains: one set was from genital lesions (genital site collection), and the other was from non-genital lesions (non-genital site collection). Discriminant analysis was carried out on the three sets of HSV-1 strains: the criterion variable had two values of primary infection or recurrence, and the predictor variables were 20 RFLPs. The degrees of separation between primary infection and recurrence increased in the order oro-facial site collection, genital site collection, and non-genital site collection. The results of discriminant analysis in this study confirmed that reactivation of HSV-1 infection is influenced by triggering factors and the site of infection, thereby suggesting the capabilities of multivariate analysis (including discriminant analysis) with DNA polymorphisms for molecular epidemiological studies.
    Journal of Virological Methods 03/2007; 139(2):159-65. · 1.90 Impact Factor
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    ABSTRACT: Synovial sarcoma is rare in the head and neck, especially in the oral and maxillofacial region. We report a case of synovial sarcoma of the mandible in a 42-year-old man. The patient was referred with recurrent diffuse swelling in the right buccal area and underwent regional resection of the right mandibular bone with construction. He has been alive and well with no evidence of disease for seven years since final surgery. The histological features are described and diagnostic problems discussed.
    Oral Oncology Extra 01/2005; 41(3):45-48.
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    ABSTRACT: A 9-month-old boy with Coffin Siris syndrome is described. Characteristic features include eyebrow hypertrichosis, long eyelashes, flat nasal bridge, prominent philtrum, hypoplasia or absence of the distal phalanges, high palate, and enlarged gingiva. The child's condition worsened and he ultimately died of multiple organ failure at the age of 5 years and 3 months.
    Asian Journal of Oral and Maxillofacial Surgery 09/2003; 15(3):205–207.
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    ABSTRACT: Hepatitis C virus (HCV) infection not only causes chronic liver diseases but shows extrahepatic manifestations as oral lichen planus (OLP) and oral cancer. To elucidate the direct relationships among these diseases and HCV infection, we investigated the detection of positive- and negative-strand HCV-RNA from serum, OLP (n=19), and oral cancer (n=17) tissues. We used a sensitive reverse transcription to polymerase chain reaction (RT-PCR) method, and analyzed sequences from the HCV E1/E2 region of the genome from serum and tissue. Positive and negative HCV-RNA strands were observed in 13 (92.9%) and 3 (21.4%) OLP tissues, respectively. In oral cancer tissues, positive HCV-RNA strands were detected in all tissues from anti-HCV positive patients. Negative HCV-RNA strands were observed in 5 of 7 (71.4%) patient’s tissues. Furthermore, it was confirmed that the sequence from one of each OLP and oral cancer patient differed between serum and tissue HCV-RNA. These results may indicate that HCV persists and replicates in these lesions, suggesting a pathological role for HCV, although the mechanisms are unclear.
    Journal of Oral Pathology and Medicine 01/2002; 29(6):259 - 266. · 2.06 Impact Factor
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    ABSTRACT: Psychological stress has an influence on natural killer cell (NK) activity, which plays a central role in protection against microbial infection and cancer. Anxiety concerning cancer is a typical type of psychological stress observed in patients and is associated with various diseases. In this study, we examined whether anxiety about cancer reduces the NK activity or quality of life (QOL), or both, of outpatients. NK activity was determined by means of a 4-hour 51 chromium-release assay, and a QOL questionnaire by the World Health Organization called QOL-BRIEF was also used. One hundred forty-four patients were asked a variety of questions about anxiety with respect to cancer and pain on their first visit to the outpatient clinic of our Department of Oral Surgery. The NK activity in patients with anxiety about cancer was significantly lower (P < .001) than that in those with no such anxiety. In contrast, NK activity was not influenced by any other factor tested, including differences in diseases and QOL scores. Anxiety about cancer may be one of the primary factors for suppression of NK activity in oral surgery outpatients.
    Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontology 06/2001; 91(6):654-8.
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    ABSTRACT: We recently reported the SART3 tumour-rejection antigen as possessing tumour epitopes capable of inducing HLA-class I-restricted cytotoxic T lymphocytes (CTLs). This study investigated expression of the SART3 antigen in breast cancer to explore an appropriate molecule for use in specific immunotherapy of breast cancer patients. The SART3 antigen was detected in all of the breast cancer cell lines tested, 30 of 40 (75%) breast cancer tissue samples, and 0 of 3 non-tumourous breast tissue samples. SART3 derived peptides at positions 109-118 and 315-323 induced HLA-A24 restricted CTLs that reacted to breast cancer cells from the peripheral blood mononuclear cells (PBMCs) of breast cancer patients. Therefore, the SART3 antigen and its peptides could be an appropriate molecule for use in specific immunotherapy of the majority of HLA-A24-positive breast cancer patients.
    British Journal of Cancer 05/2001; 84(7):915-9. · 5.08 Impact Factor
  • J Kusukawa, F Ryu, T Kameyama, E Mekada
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    ABSTRACT: Because CD9 is implicated in cell growth, cell adhesion and cell motility, altered CD9 expression might be involved in cancer invasion and metastasis. We have studied the immunolocalization of CD9 in oral squamous cell carcinoma (SCC). Sections prepared from paraffin-embedded specimens from patients with SCC of the oral cavity were stained with a monoclonal anti-CD9 antibody by means of the streptoavidin biotin method. Significant reduction or complete loss of CD9 expression was observed in cancer cells at the periphery of the cancer nests in the advancing front of invading tumor. Among 78 cases of oral SCCs examined, 46 (59.0%) cases were completely negative for CD9 expression. Loss of CD9 expression in cancer tissue strongly correlated with a high incidence of cervical lymph node metastasis and poorer prognosis (P=0.001). Thus a close examination of CD9 in SCC tissue would be useful for the prognosis of patients with oral carcinoma.
    Journal of Oral Pathology and Medicine 03/2001; 30(2):73-9. · 2.06 Impact Factor
  • Y Nagao, M Sata, M Kage, T Kameyama, T Ueno
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    ABSTRACT: Background: In recent years, it has been suggested that oral lichen planus (OLP), a chronic inflammatory keratotic lesion, is related to hepatitis C virus (HCV) infection. Therefore, we evaluated whether the presence or absence of HCV infection caused any histopathological differences in OLP tissues. Methods; The subjects consisted of 31 patients with HCV-related liver disease complicated by OLP (32 OLP lesions) and ten OLP patients without complications due to either HCV infection or liver disease (control). A histopathological evaluation was performed in these patients. In addition, immunostaining was done on nine OLP tissues infected with HCV and on six OLP tissues without HCV infection in order to evaluate lymphocyte subsets (T cells or B cells) infiltrating into topical regions with OLP. Furthermore, the severity of hepatic fibrosis and inflammation was evaluated in liver tissues obtained by liver biopsy from six patients with HCV-related liver disease to evaluate whether there were any relationships between the severity of hepatic fibrosis or inflammation and OLP tissues. Results: There were no significant differences in the histopathological characteristics specific to OLP or in the ratios of T and B cells among infiltrating lymphocytes regardless of the presence or absence of HCV infection. Moreover, there were no certain relationships between the severity of hepatic fibrosis or inflammation and the severity of lymphocytic infiltration in OLP. Conclusions: HCV infection does not appear to influence the histopathological and immunohistochemical features of OLP.
    European Journal of Internal Medicine 11/2000; 11(5):277-282. · 2.05 Impact Factor
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    ABSTRACT: CD9 and CD63 belong to a tetramembrane-spanning glycoprotein family called tetraspanin, and are involved in a wide variety of cellular processes, but the structure-function relationship of this family of proteins has yet to be clarified. CD9 associates with diphtheria toxin receptor (DTR), which is identical to the membrane-anchored form of heparin-binding EGF-like growth factor (proHB-EGF). CD9 upregulates the diphtheria toxin (DT) binding activity of DTR/proHB-EGF, while CD63 does not upregulate the DT binding activity in spite of the fact that this protein also associates with DTR/proHB-EGF on the cell surface. CD9 molecules localize on the cell surface, while those of CD63 localize predominantly at lysosomes and intracellular compartments. We made CD9/CD63 chimeric molecules and then studied their intracellular localization and upregulation activities. The C-terminal regions of CD63, which includes the lysosome sorting motif, showed a strong inhibitory effect on the expression of the chimeric proteins at the cell surface, while mutants lacking the lysosome sorting motif delivered more efficiently on the cell surface, indicating that the lysosome sorting motif contributes to the inhibitory effect of the C-terminal region. However, the N-terminal half of this family of proteins containing the 1st to 3rd transmembrane domains also seems to influence the cell surface expression. For the upregulation of DT binding activity the large extracellular loop (EC2) of CD9 was essential, while the remaining regions influenced the upregulation activity by changing the efficiency of cell surface expression. From these results we discussed the structure-function relationship of this family of proteins.
    Cell Structure and Function 11/2000; 25(5):317-27. · 1.65 Impact Factor
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    ABSTRACT: To examine whether cancer cell dissemination results from incisional biopsy, we tried to detect squamous cell carcinoma (SCC) cells in peripheral blood before and after incisional biopsy by means of cytokeratin 19 (CK19) reverse-transcriptase polymerase chain reaction (RT-PCR). The study population consisted of 20 patients with oral SCC; 10 were given incisional biopsies followed by radical excision (the incisional biopsy group), and the remaining 10 were treated by excisional biopsy alone (the excisional biopsy group). Ten non-oral cancer patients with benign oral lesions served as controls. Five-ml blood aspirates collected before and after incision were used for CK19 RT-PCR. Two (20.0%) of 10 patients from the incisional biopsy group were positive for CK19 transcripts in their peripheral blood drained 15 min after incision. In contrast, CK19 transcript was not detected either in the excisional biopsy group or in controls. Surgical invasiveness for oral cancer, including incisional biopsy, causes dissemination of cancer cells into circulation, resulting in increased risk of metastasis.
    Journal of Oral Pathology and Medicine 09/2000; 29(7):303-7. · 2.06 Impact Factor
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    ABSTRACT: Hepatitis C virus (HCV) infection not only causes chronic liver diseases but shows extrahepatic manifestations as oral lichen planus (OLP) and oral cancer. To elucidate the direct relationships among these diseases and HCV infection, we investigated the detection of positive- and negative-strand HCV-RNA from serum, OLP (n=19), and oral cancer (n=17) tissues. We used a sensitive reverse transcription to polymerase chain reaction (RT-PCR) method, and analyzed sequences from the HCV El/E2 region of the genome from serum and tissue. Positive and negative HCV-RNA strands were observed in 13 (92.9%) and 3 (21.4%) OLP tissues, respectively. In oral cancer tissues, positive HCV-RNA strands were detected in all tissues from anti-HCV positive patients. Negative HCV-RNA strands were observed in 5 of 7 (71.4%) patient's tissues. Furthermore, it was confirmed that the sequence from one of each OLP and oral cancer patient differed between serum and tissue HCV-RNA. These results may indicate that HCV persists and replicates in these lesions, suggesting a pathological role for HCV, although the mechanisms are unclear.
    Journal of Oral Pathology and Medicine 08/2000; 29(6):259-66. · 2.06 Impact Factor
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    ABSTRACT: As society ages, the composition of the diseases that occur within it changes accordingly. With that in mind, we examined the characteristics and trends in the recent inpatients and compared these recent inpatients with those of a previous report to identify the changes that accompany the aging of society. Subjects were 1,534 cases (men 56.9%, female 43.1%, average age 47.1 years) who were hospitalized at Kurume University Hospital for treatment during the 5-year period from January 1st, 1994 through December 31st, 1998. The ratio of inpatients over 65 years old was about 1.8 times higher than in the previous study, showing a clear trend toward an increased overall age of inpatients. As for the types of disease observed, the most common malignancies were epithelial tumor, followed by other benign tumors, as well as 76 cases which included diseases resembling tumor (epulis and exostosis etc.). A majority of the patients (55.6%) were directed to the Hospital by their dentist, a finding similar to that of the previous report. As for geographical distribution, 93.3% of the inpatients lived within 40 km of the center of Kurume City where our oral surgery is located, an increase of about 10% from the last report. In other words, our results showed a reduction in the sphere of treatment distribution.
    The Kurume Medical Journal 02/2000; 47(4):291-7.
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    ABSTRACT: It has been reported that hepatitis C virus (HCV) causes not only liver disease but also disorders of other organs and tissues. Previously, many HCV-related extrahepatic manifestations have been reported. In this study, we report 2 patients in whom tongue cancer was detected during the treatment of HCV-related liver disease. In one patient, tongue cancer was detected during the treatment of HCV-related liver cirrhosis, and articular rheumatism developed thereafter. The duration of HCV-related liver disease was 10 years. In the other patient, tongue cancer was detected during the treatment of HCV-related hepatocellular carcinoma. This patient had a past history of thyroid disease. The duration of HCV-related liver disease was 6 years. In these patients, the possibility that several conditions incidentally and concurrently developed cannot be denied. However, the conditions described above may be regarded as HCV-related extra-hepatic manifestations. In patients with HCV infection, it is important to examine conditions in organs other than the liver. Careful follow-up is needed.
    International Journal of Molecular Medicine 01/2000; 4(6):621-5. · 1.96 Impact Factor
  • Y Nagao, M Sata, H Suzuki, T Kameyama, T Ueno
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    ABSTRACT: GASTROENTEROLOGY 1999;117:283-284
    Gastroenterology 08/1999; 117(1):283-4. · 12.82 Impact Factor
  • O Iwamoto, H Harada, J Kusukawa, T Kameyama
    Journal of Oral and Maxillofacial Surgery 04/1999; 57(3):338-41. · 1.33 Impact Factor
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    ABSTRACT: Pemphigus vulgaris is a disseminated disease of the skin and mucous membranes characterized by recurrent vesicular and bullous lesions due to the autoantigen belonging to the cadherin type of cell adhesion molecules. The presence of acantholysis associated with immunoglobulins in the intercellular spaces and on the cell membrane are diagnostic features. However, the appearance of smears from the oral cavity by scanning (SEM) and transmission electron microscopic (TEM) study as well as immunocytochemistry of cadherin does not appear to have been previously reported. A 67-year-old female developed erosion on her gingiva with severe pain. On oral examination, there were ulcerations on the palate, and the Nikolsky sign was positive. The characteristic cytologic findings from oral scrapes were high cellularity, a bloody background and a predominant cell population consisting of polygonal basal and parabasal cells with pronounced nucleoli. Also present were degenerative cell changes: e.g., cytoplasmic vacuoles and a homogeneous nuclear appearance. Immunocytochemical staining for IgG and cadherin gave a positive reaction in the intercellular spaces and on the cell membranes. The surface of cells in pemphigus vulgaris by SEM showed somewhat irregularly distributed microridges, and TEM revealed desmosomal attachments, degenerated tonofilaments with pronounced nucleoli and heterochromatin. As a result of cytodiagnosis, additional appropriate specimens were obtained at the time of the scraping for confirmatory immunocytochemistry for cadherin, SEM and TEM studies. The results demonstrate that a precise diagnosis of pemphigus vulgaris can be rendered on cellular material and cadherin immunocytochemistry obtained by scrape from the oral mucosa.
    Acta cytologica 01/1999; 43(2):289-94. · 0.69 Impact Factor
  • Acta Cytologica - ACTA CYTOL. 01/1999; 43:289-294.
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    ABSTRACT: The MAGE-4 gene, a member of the MAGE gene family, is expressed in various cancers, including head-and-neck squamous-cell carcinomas (HN-SCC), but is not expressed in any normal tissues except for the testis and placenta. The aim of this study was to determine whether serum MAGE-4 protein is a useful tumor marker for detection of HN-SCC. An enzyme-linked immunosorbent assay was used to measure serum level of MAGE-4 protein. The serum level of MAGE-4 in pre-operative HN-SCC patients was significantly higher than that in patients with non-malignant diseases (NMD) of the head and neck, volunteers undergoing cancer screening (VOL), or healthy donors (HD). When the cut-off level was determined at 1.15 ng/ml (mean plus 3 SD of HD), sera from 28 of 96 patients with HN-SCC (p < 0.0001 vs. the other groups), 7 of 82 patients with NMD, 2 of 92 with VOL, and 0 of 68 HD were positive for MAGE-4. Serum levels of MAGE-4 protein in all 7 HN-SCC patients whose sera were positive for MAGE-4 before operation decreased after operation, and, in one patient, a renewed rise in serum level was followed by recurrence. These results indicate that MAGE-4 protein is detectable in sera of a significant number of HN-SCC patients, and that serum MAGE-4 protein might be a useful tumor marker to monitor the recurrence of MAGE-4-positive HN-SCC. Int. J. Cancer, 70:287–290, 1997. © 1997 Wiley-Liss, Inc.
    International Journal of Cancer 12/1998; 70(3):287 - 290. · 6.20 Impact Factor
  • Y Nagao, T Kameyama, M Sata
    The American Journal of Gastroenterology 06/1998; 93(5):850. · 7.55 Impact Factor