Jinhua Xu

Fudan University, Shanghai, Shanghai Shi, China

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Publications (37)149.33 Total impact

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    ABSTRACT: The seasonality of varicella and herpes zoster has been widely observed, but there is limited evidence on their associations with ambient temperature. The associations between ambient temperature and outpatient visits for varicella and herpes zoster were examined. We collected daily outpatient visit data for varicella (N = 3520) and herpes zoster (N = 6614) from a major hospital in Shanghai, China, from 2008 to 2010. We adopted the generalized additive model in time-series analysis to investigate the short-term associations between temperature and outpatient visits for varicella and herpes zoster after controlling for seasonal trends, day of the week, and humidity. A 1°C increase in daily mean temperature was associated with a 1.33% (95% confidence interval 0.93%-1.74%) decrease in outpatient visits for varicella whereas it was associated with a 2.18% (95% confidence interval 1.90%-2.46%) increase in outpatient visits for herpes zoster. Only 1 hospital was included and we did not control for individual-level risk factors. Our results demonstrated that temperature variation might be an important risk factor for varicella and herpes zoster in China. Copyright © 2015 American Academy of Dermatology, Inc. Published by Elsevier Inc. All rights reserved.
    Journal of the American Academy of Dermatology 08/2015; DOI:10.1016/j.jaad.2015.07.015 · 5.00 Impact Factor
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    ABSTRACT: It has been suggested that the single nucleotide polymorphism (SNP) of the CYP19A1 gene encoding aromatase may affect the development of female pattern hair loss (FPHL). Our aim was to investigate the association of CYP19A1 gene SNPs with FPHL in a Chinese population. Two hundred Chinese Han patients with FPHL and 200 controls were enrolled into our study. SNaPshot technology was used to detect CYP19A1 gene candidate SNPs. The allele frequencies and distributions of rs6493497 and rs7176005 were significantly different between FPHL and control subjects (p < 0.001 and p < 0.001 vs. p < 0.001 and p = 0.003). The rs6493497 and rs7176005 SNPs of the CYP19A1 gene may be genetic markers that influence the risk of FPHL in this Chinese population. © 2015 The Author(s) Published by S. Karger AG, Basel.
    Dermatology 07/2015; DOI:10.1159/000433597 · 1.69 Impact Factor
  • Zhihua Kang · Qiao Li · Pan Fu · Shuxian Yan · Ming Guan · Jinhua Xu · Feng Xu
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    ABSTRACT: Atopic dermatitis (AD) is the most common chronic inflammatory skin disease in Chinese pediatric patients. To date, the genetic susceptibility to AD in this population has not been fully clarified. Three single nucleotide polymorphisms have previously been associated with AD in Europeans, rs2897442 (KIF3A), rs479844 (OVOL1) and rs2164983 (ACTL9). To verify the correlation between AD and these three SNPs in the Chinese pediatric population, we conducted a case-control study including 235 pediatric patients with AD and 200 health controls. We confirmed the correlation between rs2897442 and rs479844 and AD in this population at both the genotype and allele levels. Statistical analysis showed that the C allele of rs2897442 is associated with an increased risk of developing AD, while the A allele of rs479844 is associated with a reduced risk. No correlation between rs2164983 and AD was identified. Our study indicates that KIF3A and OVOL1 are involved in the development of AD in the Chinese pediatric population. Copyright © 2015. Published by Elsevier B.V.
    Gene 06/2015; DOI:10.1016/j.gene.2015.06.068 · 2.08 Impact Factor
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    ABSTRACT: Calcipotriol/betamethasone dipropionate combination in a non-alcoholic, lipophilic gel formulation (two-compound gel) has previously been demonstrated as a safe and effective treatment for scalp psoriasis in Caucasian, Hispanic/Latino, and Black/African American populations. The purpose of this randomized, investigator-blinded, active-controlled, 4-week study was to evaluate the efficacy and safety of the two-compound gel in Chinese subjects with scalp psoriasis. Subjects were randomized in a 1 : 1 ratio to four weeks of treatment with either the two-compound gel once daily or calcipotriol scalp solution twice daily. Subjects were evaluated after one, two, and four weeks of treatment. The primary efficacy endpoint was the proportion of subjects who achieved "controlled disease" defined as "clear" or "minimal" disease according to investigator's global assessment of disease severity at week 4. The proportion of subjects who achieved "controlled disease" at week 4 was statistically significantly higher in the two-compound gel group (87.5%) than in the calcipotriol solution group (50.8%), (P < 0.0001). Greater and more rapid improvements with the two-compound gel were also observed in clinical signs (redness, thickness, and scaliness) and itching. The two-compound gel was associated with fewer adverse drug reactions than calcipotriol scalp solution (18.6% vs. 33.1%) (P = 0.011). The calcipotriol/betamethasone dipropionate gel applied once daily was significantly more effective and better tolerated than calcipotriol scalp solution applied twice daily in the treatment of scalp psoriasis over four weeks in Chinese subjects. © 2015 The International Society of Dermatology.
    International journal of dermatology 06/2015; DOI:10.1111/ijd.12788 · 1.23 Impact Factor
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    ABSTRACT: Background Occupational toxic epidermal necrolysis (TEN) related to Dalbergia cochinchinensis has seldom been reported in the past. Its clinical characteristic needs to be investigated. This study reports eight cases of such disease in China.Methods Eight patients with occupational TEN admitted from 2003 to 2012 were retrospectively analyzed and compared with 15 patients admitted with TEN caused by drugs as controls. Patients all received combination therapy of corticosteroid and intravenous immunoglobulin. The times for bullous ceasing, tapering of corticosteroid, and total hospitalization were compared between the two groups of patients. SCORTEN, a severity-of-illness scoring system for TEN prognosis, was applied to evaluate clinical outcome.ResultsThe three time measurements in occupational TEN were longer than those in control, and the differences were statistically significant (P = 0.0023, 0.026, 0.0017), which means the total dose of corticosteroid needed in occupational TEN was higher than that in the control. There were no deaths in the two groups, although expected deaths were 0.612 and 0.836, respectively.DiscussionOccupational TEN has a longer progression than TEN caused by drugs, and there is more difficulty in its treatment. Clinicians should pay attention to this disease. However, its mechanism and target therapy remain unclear.
    International journal of dermatology 05/2015; DOI:10.1111/ijd.12784 · 1.23 Impact Factor
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    ABSTRACT: Genome-wide association studies (GWASs) have reproducibly associated ∼40 susceptibility loci with psoriasis. However, the missing heritability is evident and the contributions of coding variants have not yet been systematically evaluated. Here, we present a large-scale whole-exome array analysis for psoriasis consisting of 42,760 individuals. We discover 16 SNPs within 15 new genes/loci associated with psoriasis, including C1orf141, ZNF683, TMC6, AIM2, IL1RL1, CASR, SON, ZFYVE16, MTHFR, CCDC129, ZNF143, AP5B1, SYNE2, IFNGR2 and 3q26.2-q27 (P<5.00 × 10(-08)). In addition, we also replicate four known susceptibility loci TNIP1, NFKBIA, IL12B and LCE3D-LCE3E. These susceptibility variants identified in the current study collectively account for 1.9% of the psoriasis heritability. The variant within AIM2 is predicted to impact protein structure. Our findings increase the number of genetic risk factors for psoriasis and highlight new and plausible biological pathways in psoriasis.
    Nature Communications 04/2015; 6:6793. DOI:10.1038/ncomms7793 · 10.74 Impact Factor
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    ABSTRACT: GPP, generalized pustular psoriasis; LD, linkage disequilibrium; MAF, minor allele frequency; OR, odds ratio; PV, psoriasis vulgaris; SNP, single nucleotide polymorphism
    Journal of Investigative Dermatology 03/2015; DOI:10.1038/jid.2015.111 · 6.37 Impact Factor
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    International Journal of Environmental Research and Public Health 03/2015; 12(3):3232-3245. DOI:10.3390/ijerph120303232 · 2.06 Impact Factor
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    ABSTRACT: Various preparations of botulinum toxin type A (BTX-A) are used to reduces glabellar (frown) lines. However, dose-related safety and efficacy of intramuscular injections of a widely used, locally produced BTX-A in China has not been established. Assessment of dose-dependent safety and efficacy of Chinese botulinum toxin type A (Hengli BTX-A [HBTX-A]) intramuscular injections on glabellar lines. Four hundred eighty-eight BTX-A-naive participants were included in the double-blind trial and randomly divided into placebo (n = 122), low-dose (n = 183), and high-dose (n = 183) treatment groups for injection with saline solution, 10 units and 20 units of HBTX-A, respectively, at 4 sites in the corrugator muscle and 1 site in the procerus muscle. Outcomes were recorded before treatment and after 7, 30, 60, and 120 days, including glabellar line severity at maximum contraction and relaxation. Significantly greater improvement was observed in both HBTX-A groups in comparison with the placebo group (p < .05). Better efficacy was obtained in the high-dose treatment group. More participants developed adverse events after treatment with HBTX-A doses, than with the placebo (p < .05). Twenty-unit HBTX-A provided optimal improvement in glabellar lines, and its use might minimize injection frequency while maintaining acceptable safety.
    Dermatologic Surgery 01/2015; 41 Suppl 1:S56-63. DOI:10.1097/DSS.0000000000000265 · 1.56 Impact Factor
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    ABSTRACT: Myeloid-derived suppressor cells (MDSCs) are a heterogeneous population of cells that consists of myeloid progenitor cells and immature myeloid cells. They have been identified as a cell population that may affect the activation of CD4+ and CD8+ T-cells to regulate the immune response negatively, which makes them attractive targets for the treatment of transplantation and autoimmune diseases. Several studies have suggested the potential suppressive effect of MDSCs on allo- and autoimmune responses. Conversely, MDSCs have also been found at various stages of differentiation, accumulating during pathological situations, not only during tumor development but also in a variety of inflammatory immune responses, bone marrow transplantation, and some autoimmune diseases. These findings appear to be contradictory. In this review, we summarize the roles of MDSCs in different transplantation and autoimmune diseases models as well as the potential to target these cells for therapeutic benefit.
    Mediators of Inflammation 01/2015; 2015:1-14. DOI:10.1155/2015/421927 · 3.24 Impact Factor
  • Duoqin Wang · Jun Liang · Jinhua Xu · Lianjun Chen
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    ABSTRACT: A case of D-penicillamine(DPA) induced elastosis perforans serpiginosa(EPS) in a 32-year-old Chinese man was reported. The presentation lasted two years and was refractory to traditional medical treatment. He was then commenced on 7.6% 5-aminolevulinic acid (ALA) induced photodynamic therapy(PDT) by a LED light of 633 nm at dose levels of 130J/ cm(2) for each session with total 3 sessions at one week interval. The patient was tolerated and responded well to this new approach for DPA-induced EPS without any adverse events. The etiology, pathophysiology, natural history, and treatment options for DPA-induced EPS are reviewed, and the authors suggest this method of treatment to be effective and safe for patients of DPA-induced EPS refractory to conventional therapy.
    Photodiagnosis and Photodynamic Therapy 11/2014; 12(1). DOI:10.1016/j.pdpdt.2014.11.001 · 2.52 Impact Factor
  • Jinfeng Wu · Tao Song · Shuyong Liu · Xiaomei Li · Gang Li · Jinhua Xu
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    ABSTRACT: Icariside II (IS) is a metabolite of icariin, which is derived from Herba Epimedii. In the present study, the antiproliferative effects of IS on A375 human melanoma cells were examined in vitro and a possible mechanism through the ROS‑p38‑p53 pathway is discussed. A cell WST‑8 assay revealed that treatment with IS markedly reduced cell viability from 77 to 21% (25 and 100 µM, respectively), and cell counting demonstrated that IS treatment reduced cell proliferation. IS treatment also induced cell cycle arrest of A375 cells at the G0/G1 and G2/M transitions and inhibited the expression of cell‑cycle related proteins, including cyclin E, cyclin‑dependent kinase 2 (CDK2), cyclin B1 and phosphorylated cyclin‑dependent kinase 1 (P‑CDK1). In this study, it was determined that IS inhibits cell proliferation and induces cell cycle arrest through the generation of reactive oxygen species and activation of p38 and p53. These findings were further supported by the evidence that pretreatment with N‑acetyl‑L‑cysteine, SB203580 or pifithrin‑α significantly blocked IS‑induced reduction of cell viability, increase of cell death and cell cycle arrest. In conclusion, IS inhibits cell proliferation and induces cell cycle arrest. Crucially, it was confirmed that these effects were mediated at least in part by activating the ROS‑p38‑p53 pathway.
    Molecular Medicine Reports 10/2014; 11(1). DOI:10.3892/mmr.2014.2701 · 1.48 Impact Factor
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    ABSTRACT: Background 5-aminolevulinic acid (5-ALA), a precursor of heme biosynthesis, plays a fundamentally important role in aerobic energy metabolism. Heme oxygenase (HO)-1 cleaves heme to form biliverdin, carbon monoxide (CO) and iron (Fe2+). The anti-inflammatory properties of biliverdin and CO help to alleviate ischemia/reperfusion injury as well as acute and/or chronic allograft rejection. We herein investigated whether 5-ALA and Fe2+ exerts salutary effects in the setting of organ transplantation. Methods An in vitro mixed lymphocyte reaction (MLR) assay and cardiac allotransplantation model (CBA to C57BL/10) were used to evaluate the effects of 5-ALA and Fe2+ on transplantation tolerance. Results Treatment with 5-ALA and sodium ferrous citrate (SFC) resulted in permanent acceptance in the murine cardiac allografts in a dose-, SFC- and HO-1-dependent manner. The number of graft infiltrating CD8 T cells and the survival response of the recipient spleen T cells to the donor type alloantigens were lower than those observed in the control recipients; however, the number of both regulatory T cells and dendritic cells was significantly increased in the 5-ALA/SFC-treated recipients. Conclusions These data indicate that 5-ALA/SFC inhibits T cell proliferation in response to alloantigens and an increased number of regulatory cells, resulting in permanent cardiac allograft acceptance in mice. These findings highlight the important roles of CO and/or HO-1 in inducing tolerance and imply that 5-ALA/SFC may become a clinically effective treatment for allograft rejection.
    The Journal of Heart and Lung Transplantation 10/2014; 34(2). DOI:10.1016/j.healun.2014.09.037 · 5.61 Impact Factor
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    ABSTRACT: In a previous large-scale exome sequencing analysis for psoriasis, we discovered seven common and low-frequency missense variants within six genes with genome-wide significance. Here we describe an in-depth analysis of noncoding variants based on sequencing data (10,727 cases and 10,582 controls) with replication in an independent cohort of Han Chinese individuals consisting of 4,480 cases and 6,521 controls to identify additional psoriasis susceptibility loci. We confirmed four known psoriasis susceptibility loci (IL12B, IFIH1, ERAP1 and RNF114; 2.30 × 10(-20)≤P≤2.41 × 10(-7)) and identified three new susceptibility loci: 4q24 (NFKB1) at rs1020760 (P=2.19 × 10(-8)), 12p13.3 (CD27-LAG3) at rs758739 (P=4.08 × 10(-8)) and 17q12 (IKZF3) at rs10852936 (P=1.96 × 10(-8)). Two suggestive loci, 3p21.31 and 17q25, are also identified with P<1.00 × 10(-6). The results of this study increase the number of confirmed psoriasis risk loci and provide novel insight into the pathogenesis of psoriasis.
    Nature Communications 07/2014; 5:4331. DOI:10.1038/ncomms5331 · 10.74 Impact Factor
  • Yanyun Shen · Jinhua Xu · Jin Jin · Hui Tang · Jun Liang
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    ABSTRACT: Cyclin D1 is a member of the G1 cyclin family that regulates the transition through the G1 phase of the cell cycle and is involved in the neoplastic transformation of certain tumors. This study was designed to investigate the expression of cyclin D1 in Bowen's disease (BD) and cutaneous squamous cell carcinoma (SCC). Biopsies of 30 cases with BD and 24 cases with SCC confirmed by histopathology were obtained from the Department of Dermatology of Huashan Hospital, Shanghai, China. EnVision immunohistochemical technology with a semiquantitative immunohistochemical score was applied to detect the expression of cyclin D1. Of the 24 specimens with SCC, cyclin D1 was found to be positive in 17 (70.8%), whereas of the 30 specimens with BD, cyclin D1 was found to be positive in 13 (43.3%). The expression of cyclin D1 was significantly higher in the SCC compared to that in the BD group. We did not observe a significant association of cyclin D1 expression with different pathological grades of SCC. In conclusion, cyclin D1 plays a significant role as a diagnostic marker in skin tumors and its overexpression was not found to be correlated with the degree of differentiation of SCC.
    Molecular and Clinical Oncology 07/2014; 2(4):545-548. DOI:10.3892/mco.2014.273
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    ABSTRACT: Regulatory dendritic cells (DCregs) represent a potential therapeutic tool for assessing a variety of immune overreaction conditions; however, current approaches for generating DCregs for therapeutic purposes are limited. We attempted to generate and characterize DCregs from murine induced pluripotent stem (iPS) cells. The iPS cells co-cultured with OP9 cells displayed mesodermally differentiated flat colonies. GM-CSF drove most of the colonies exhibiting a differentiated morphology. Thereafter, cells became morphologically heterologous under the effects of TGF-β and IL-10. Most of the floating cells developed an irregular shape with areas of protrusion. The generated iPS-DCregs demonstrated high CD11b/c and low CD40, CD80, CD86 and MHC-II expressions with a high antigen uptake ability and poor T-cell stimulatory function. Importantly, iPS-DCregs showed immune responsiveness regulation effects both in vitro and in vivo and the ability to generate regulatory T-cells in vitro. Our result illustrates a feasible approach for generating functional DCregs from murine iPS cells.
    Scientific Reports 02/2014; 4:3979. DOI:10.1038/srep03979 · 5.58 Impact Factor
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    ABSTRACT: Icariin is the major active ingredient of Herba Epimedii. Icaritin (ICT) is a hydrolytic product of Icariin. In the present study, we investigated the protective role of ICT against cigarette smoke extract (CSE)-mediated oxidative stress in human lung epithelial A549 cells. As demonstrated by the WST-8 assay, exposure to CSE (2.5%, 5%, and 10%) reduced the cell viability of A549 cells (84%, 64% and 53%) in a dose-dependent manner and treatment with ICT10μM dramatically attenuated CSEinduced cytotoxicity (73% and 64%). The MFI data suggested that CSE induced oxidative stress by generating ROS(230)and 10μM ICT treatment attenuated CSE-induced ROS production(90). 10μM ICT treatment resulted in significant AKT activation, Nrf2 nuclear translocation, increased GCL transcription and GSH levels, as compared with CSE exposure alone. However, ICT-mediated upregulation of GCL transcription in CSE-treated cells were lost in Nrf2 siRNA-transfected cells. Furthermore, inhibition of PI3K/AKT signaling by LY294002 partially prevents ICT-induced nuclear translocation of Nrf2 and GCL transcription. These findings suggest that ICT attenuates CS-induced oxidative stress by quenching ROS and also by upregulating GSH via a PI3K-AKT-Nrf2-dependent mechanism. Further studies are required to confirm that a similar protective effect of ICT occurs in the lungs in vivo in response to CS exposure.
    Food and chemical toxicology: an international journal published for the British Industrial Biological Research Association 12/2013; 64. DOI:10.1016/j.fct.2013.12.006 · 2.61 Impact Factor
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    ABSTRACT: To explore the contribution of functional coding variants to psoriasis, we analyzed nonsynonymous single-nucleotide variants (SNVs) across the genome by exome sequencing in 781 psoriasis cases and 676 controls and through follow-up validation in 1,326 candidate genes by targeted sequencing in 9,946 psoriasis cases and 9,906 controls from the Chinese population. We discovered two independent missense SNVs in IL23R and GJB2 of low frequency and five common missense SNVs in LCE3D, ERAP1, CARD14 and ZNF816A associated with psoriasis at genome-wide significance. Rare missense SNVs in FUT2 and TARBP1 were also observed with suggestive evidence of association. Single-variant and gene-based association analyses of nonsynonymous SNVs did not identify newly associated genes for psoriasis in the regions subjected to targeted resequencing. This suggests that coding variants in the 1,326 targeted genes contribute only a limited fraction of the overall genetic risk for psoriasis.
    Nature Genetics 11/2013; 46(1). DOI:10.1038/ng.2827 · 29.65 Impact Factor
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    ABSTRACT: Melanoma is the deadliest cutaneous malignancy because of its high incidence of metastasis. Melanoma growth and metastasis relies on sustained angiogenesis; therefore, inhibiting angiogenesis is a promising approach to treat metastatic melanoma. JWA is a novel microtubule-associated protein and our previous work revealed that JWA inhibited melanoma cell invasion and metastasis. However, the role of JWA in melanoma angiogenesis and the prognostic value are still unknown. Here, we report that JWA in melanoma cells significantly inhibited the tube formation of endothelial cells. In addition, JWA regulated ILK through intergrin αVβ3 and such regulation was achieved through the transcription factor Sp1. Notably, both in vitro and in vivo angiogenesis assays revealed that JWA dramatically suppressed melanoma angiogenesis by inhibiting ILK signaling. Furthermore, we examined the expression of JWA protein in a large set of melanocytic lesions (n=505) at different stages by tissue microarray and found an inverse correlation between JWA expression and melanoma progression (P=5×10(-6)). Importantly, reduced JWA expression was correlated with a poorer overall, and disease-specific, 5-year survival of patients (P=0.001 and 0.007, respectively). Multivariate Cox regression analyses indicated that JWA was an independent prognostic marker for melanoma patients. Moreover, we found a significant negative correlation between JWA and ILK in melanoma biopsies, and their concomitant expression was closely correlated with melanoma patient survival (P=0.004), further indicating the regulation of ILK expression by JWA is critical in melanoma. Taken together, our data highlight the function of JWA in melanoma angiogenesis and reveal the clinical prognostic value of JWA.
    Carcinogenesis 09/2013; 34(12). DOI:10.1093/carcin/bgt318 · 5.27 Impact Factor
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    ABSTRACT: To assess the epidemic trends of syphilis and to investigate syphilis infections after exposure to infectious patients. A total of 17 211 syphilis patients from the period January 1999 to September 2012 were enrolled in this study. A variety of syphilis prevalence measures were evaluated. We analyzed the characteristics of 2954 cases using available information. Of these patients, 535 early syphilis cases were identified as index patients and the status of their sexual partners was monitored. All sexual partners were followed for 6 months to 1 year through serological testing and clinical examinations. The proportion of syphilis-positive clients at the sexually transmitted disease (STD) clinic increased annually, with a five-fold increase from 1999 to 2011 (from 6.1% to 30.0%). The highest increase in syphilis infection occurred among patients in the 20-29 years age group. Male and female cases increased at the same rate between 1999 and 2007, but female cases increased at a greater rate than male cases from 2008 to 2012. Of the 535 sexual partners in the study, 330 (61.7%) were infected with syphilis and 205 (38.3%) were seronegative without any symptoms. Gender may influence disease infection rates (p=0.008), but not at different stages of early syphilis. There was an increasing trend of syphilis infection in Hefei, China. A proportion of highly exposed individuals could be resistant to syphilis infection.
    International journal of infectious diseases: IJID: official publication of the International Society for Infectious Diseases 07/2013; 17(11). DOI:10.1016/j.ijid.2013.05.007 · 2.33 Impact Factor