Ken Touhata

Publications (4)2.81 Total impact

• Article: Molecular cloning and characterization of a tissue inhibitor of metalloproteinase-2 from red seabream cultured cells

  Ken Touhata, Yuki Tokuda, Michiaki Yamashita, Haruhiko Toyohara
  Fisheries Science 04/2012; 72(3):699-701. · 0.94 Impact Factor
• Article: Immunological detection of translation products of type V/XI collagen α1 gene and their degradation products in red sea bream muscle

  KEN TOUHATA, YUKI TOKUDA, MORIHIKO SAKAGUCHI, HARUHIKO TOYOHARA
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  ABSTRACT: We previously cloned cDNA of type V/XI collagen α1 chain (ColVa1) gene from cultured cells derived from red sea bream embryo. We raised an antibody against the deduced C-telopeptide of ColVa1 in order to detect the translation products of this cDNA and their degradation products in red sea bream muscle. To improve its specificity, the antibody was purified from rabbit antiserum by use of an affinity column cross-linked with recombinant C-terminal peptide of ColVa1 produced by Epicurian coli. The purified antibody recognized a band corresponding to the α chain of type V/XI collagen in western blot analysis of the extract of cultured cells. The antibody also recognized two bands in acid-soluble and pepsin-solubilized collagens, indicating that the translation products of the ColVa1 gene are present in muscle and that bands correspond to α and β chains of type V/XI collagen. A band corresponding to a molecular weight of approximately 65 k was detected in the NaOH extracts of muscle, suggesting that type V/XI collagen α1 chain is restrictedly digested in red sea bream muscle.
  Fisheries Science 10/2002; 68(5):1118 - 1123. · 0.94 Impact Factor
• Article: Seasonal change in collagen content of red seabream muscle

  Ken Touhata, Masataka Tanaka, Haruhiko Toyohara, Hideki Tanaka, Morihiko Sakaguchi
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  ABSTRACT: To investigate the correlation between firmness and the collagen content in fish muscle, we evaluated the seasonal change in collagen content of red seabream muscle on the basis of the content of protein-form hydroxyproline. Since a significant difference was observed among the ratios of proline (Pro) hydroxylation in female fish (P <0.05), the collagen content of each sample tested was converted from the ratio of Pro hydroxylation. The collagen content in male fish tended to increase from 0.96 mg/g muscle (March) to 1.27 mg/g (June), and then to decrease to 0.81 mg/g (July). In both male and female fish, the collagen contents tended to increase from July to September. Linear regression analysis revealed that there was a positive correlation between the muscle firmness and the collagen content in each month (P <0.05, male; r = 0.686, female; r = 0.742). The results suggest that the seasonal change in collagen content in muscle possibly contributes to that in firmness.
  Fisheries Science 12/2001; 66(3):553 - 557. · 0.94 Impact Factor
• Article: Sequence and expression of a cDNA encoding the red seabream androgen receptor

  Ken Touhata, Masato Kinoshita, Yuki Tokuda, Haruhiko Toyohara, Morihiko Sakaguchi, Yoshihiro Yokoyama, Shinya Yamashita
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  ABSTRACT: The cDNA of the androgen receptor (AR) has been isolated from the ovary of red seabream, Pagrus major, and sequenced. The amino acid sequence of red seabream AR (rsAR) shows about 45% identity with those of Xenopus, rat, mouse, and human ARS. It is shown that rsAR has the ability to trans-activate the responsive gene depending on the presence of androgen.
  Biochimica et Biophysica Acta (BBA) - Molecular Cell Research.