Yan Wang

Yunnan University, Yün-nan, Yunnan, China

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Publications (29)102.32 Total impact

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    ABSTRACT: Cryptomelane type manganese oxide α-MnO2 and Ni doped KMn8O16 nanostructures were synthesized by water-bathing methods at 80 °C for 24 h using NiSO4·H2O as the dopant sources. The structures, morphologies and physical properties were investigated by means of X-ray diffraction (XRD), scanning electron microscopy (SEM), transmission electron microscopy (TEM), and X-ray photoelectron spectroscopy (XPS). The results show that the products are Ni doped KMn8O16 nanorods after the introduction of NiSO4·H2O during the reaction process. The electromagnetic characteristics and microwave absorption properties of the materials were carried out with a vector network analyzer (VNA) and the transmission line (TML) theory. The dielectric loss and microwave absorption properties of the cryptomelane materials are improved after Ni doping. The thickness dependent reflection loss shows that the peak frequency and effective absorption bandwidth all decrease with the increasing material thickness. With the increase of Ni doping concentration, the peak frequency shifts to higher frequency bands and the effective absorption bandwidth increases. The electromagnetic performance of cryptomelane can be attributed to its unique tunnel structures and the improvement of Ni doping can be due to the enhanced electromagnetic polarization.
    Ceramics International 05/2015; 41(4). DOI:10.1016/j.ceramint.2014.12.153 · 2.09 Impact Factor
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    ABSTRACT: A new type of catalytic material, large-scale ZnO nanorod arrays grown on self-source substrate, was directly synthesized by a facile hydrothermal approach. The catalytic activity of the ZnO nanocrystals with different exposed surfaces, including ZnO hexagonal nanorods with exposed reactive {0001} facets, hexagonal ZnO nanopyramids with nonpolar {010} planes, and pencil-like morphology with exposed {101} polar planes, was tested towards the degradation of the azo dyes (Congo red (CR) and methyl orange (MO)). The aqueous azo dyes can be degraded efficiently under ambient conditions, requiring neither light illumination nor additional energy (agitation, ultrasonic, etc.). Systematic experiments suggested that the dye degradation proceeds through electron transfers from the anionic dye molecules to the catalyst and then to electron acceptors such as dissolved oxygen. It strongly depends on the exposed polar surfaces of the ZnO nanocrystals, giving rise to the relatively higher catalytic activity and stability of the ZnO hexagonal nanopencils. The present ZnO nanorod arrays grown on the Zn substrate require no additional reagents or external energy input, which thus provides a potentially low-cost alternative for the remediation of azo-dye effluents.
    CrystEngComm 07/2014; 16(33). DOI:10.1039/C4CE00899E · 3.86 Impact Factor
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    ABSTRACT: The transparent nanocrystalline thin films of undoped zinc oxide and Mn-doped (Zn1-xMnxO) have been deposited on glass substrates via the sol-gel technique using zinc acetate dehydrate and manganese chloride as precursor. The as-deposited films with the different manganese compositions in the range of 2.5-20 at% were pre-heated at 100 degrees C for 1 h and 200 degrees C for 2 h, respectively, and then crystallized in air at 560 degrees C for 2 h. The structural properties and morphologies of the undoped and doped ZnO thin films have been investigated. X-ray diffraction (XRD) spectra, scanning electron microscopy (SEM), atomic force microscopy (AUM), and X-ray photoelectron spectroscopy (XPS) were used to examine the morphology and microstructure of the thin films. Optical properties of the thin films were determined by photoluminescence (PL) and UV/Vis spectroscopy. The analyzed results indicates that the obtained films are of good crystal quality and have smooth surfaces, which have a pure hexagonal wurtzite ZnO structure without any Mn related phases. Room temperature photoluminescence is observed for the ZnO and Mn-doped ZnO thin films.
    Physica E Low-dimensional Systems and Nanostructures 07/2014; 61:14–22. DOI:10.1016/j.physe.2014.03.007 · 1.86 Impact Factor
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    ABSTRACT: The authors report a facile chemical precipitation method for the fabrication of a highly ordered mesoporous Mn2O3/MCM-41 composite. Examination of the acquired samples using X-ray diffraction (XRD), scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS), and nitrogen adsorption-desorption measurement has provided fundamental insight into the structure and properties of the Mn2O3/MCM-41 composite. It is found that the as-prepared Mn2O3/MCM-41 composite has a highly ordered mesoporous structure with a specific surface area of 793 m(2) g(-1). The performance of Mn2O3/MCM-41 composite as a remover was further demonstrated in the removal of azo dyes of methyl orange (MO), Congo red (CR), methylene blue (MB), and rhodamine B (RB) with/without visible light irradiation at room temperature. The results show that the Mn2O3/MCM-41 composite has an excellent removal performance for MB and RB, making it a promising candidate for wastewater treatment.
    Ceramics International 07/2014; 40(6):8093-8101. DOI:10.1016/j.ceramint.2013.12.163 · 2.09 Impact Factor
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    ABSTRACT: The coral-like ZnO-CdO composites with the 3D hierarchical porous structure were prepared in a facileflash from self- sustained decomposition of a metal- organic complex. The phases and the morphologiesof the resulting materials were characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM), transmission electron microscopy (TEM), and X-ray photoelectron spectrum (XPS), respectively. The results showed that the ZnCd composites have a coral- like shape with a size of tens of micrometersand exhibit the hierarchically porous morphology, in which the walls of the macropores contain smallermesopores. The 3D hierarchical architecture consisted of nanoparticles. The gas-sensing characteristicsof the resulted porous ZnO-CdO composites for isopropanol were investigated. Indirect- heating sensorsusing porous ZnO-CdO composites as sensitive materials were fabricated on an alumina tube with Auelectrodes and platinum wires. All measurements were performed at several operating temperaturesfrom 175 to 315 degrees C. Good gas-sensing performances to isopropanol have been found for the preparedsample of porous ZnO-CdO composite with a content of 10 at% CdO (ZnCd10). It is shown that the sensorexhibits the highest gas response and selectivity at the operating temperature of 248 degrees C, making thempromising candidates as practical detectors for isopropanol.
    Sensors and Actuators B Chemical 07/2014; 198:402-410. DOI:10.1016/j.snb.2014.03.093 · 3.84 Impact Factor
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    ABSTRACT: Well-crystalline tin oxide nanorods assembled with SnO2 nanocrystals were prepared by calcination of SnC2O4 nanorods synthesized by a chemical precipitation method using SnCl2·2H2O and PEG 400 as precursors. The phase and morphology of the resulting material were characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM), transmission electron microscopy (TEM) and X-ray photoelectron spectrum (XPS). Indirect-heating sensors using SnO2 nanorods as sensitive materials were fabricated on an alumina tube with Au electrodes and platinum wires. The as-fabricated sensor based on SnO2 nanorods showed high response, fast response and recovery toward isopropanol gas, making them promising candidates for practical detectors of isopropanol gas.
    New Journal of Chemistry 05/2014; 38(6). DOI:10.1039/C3NJ01482G · 3.16 Impact Factor
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    ABSTRACT: Lanthanum dioxide carbonate La2O2CO3 nanorods with high crystallinity were successfully prepared from La(OH)(3) nanorods. The structural and morphological characterizations of the products were performed by X-ray powder diffraction (XRD), transmission electron microscopy (TEM), and X-ray photoelectron spectrum (XPS). La(OH)(3) nanorods were shape-preserved transformed to La2O2CO3 nanorods at 400 degrees C for 2 h. TEM images indicate that the as-obtained La2O2CO3 entirely consists of uniform nanorods in high yield with diameter of 13-15 nm and length of 100-150 nm. The indirect-heating sensor using La2O2CO3 nanorods as sensitive material was fabricated on an alumina tube with Au electrodes and platinum wires. The sensing properties of the sensor based on La2O2CO3 nanorods were investigated. It is shown that the sensor exhibits high gas response for CO2 gas at operating temperature of 325 degrees C, making it to be potential candidate for practical detectors for CO2 gas. (c) 2014 Elsevier Ltd. All rights reserved.
    Electrochimica Acta 05/2014; 127:355–361. DOI:10.1016/j.electacta.2014.02.075 · 4.09 Impact Factor
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    ABSTRACT: The synthesis and the gas sensing properties of novel mixed phase (i.e., tetragonal and orthorhombic phase) coexistence SnO2 nanorods are presented. The mixed phases SnO2 nanorods were obtained by calcinations of SnC2O4 synthesized with a chemical precipitation method using SnCl2·2H2O and PEG 400 as precursors. The resulting nanorods appear as polycrystalline composed of spherical mixed phases SnO2 nanocrystals and have a high surface area. It was observed that the calcination temperature was the key parameter determining the content of the orthorhombic phase. The as-synthesized compounds were used as sensing materials of the sensors of indirect heating structure and tested for their ability to detect volatile organic compounds (VOCs), such as isopropanol, acetone, alcohol, and formaldehyde. Gas sensing tests showed that these mixed phases SnO2 nanorods are highly promising for gas sensor applications, as the gas response for isopropanol was significantly enhanced by the presence of orthorhombic phase (S = 61.5 at 1000 ppm isopropanol and response time and recovery time of 4 and 10 s). The as-prepared two phases SnO2 nanorods with the highest content of the orthorhombic phase exhibit excellent gas response, selectivity, and stability toward isopropanol gas at the optimized operating temperature of 255 °C. The enhancement in sensitivity is attributed to the presence of small orthorhombic SnO2 crystals with average radius shorther than the Debye screening length of 7 nm for SnO2.
    The Journal of Physical Chemistry C 04/2014; 118(18):9832–9840. DOI:10.1021/jp501550w · 4.84 Impact Factor
  • Ceramics International 04/2014; 40(3):5091-5095. DOI:10.1016/j.ceramint.2013.09.119 · 2.09 Impact Factor
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    ABSTRACT: Graphene nanosheets-tungsten oxides (tungsten oxide/tungsten oxide hydrate mixture) (GNS-W) composite was successfully synthesized using a facile approach. WO3/WO3·H2O mixtures were deposited on the graphene nanosheets (GNS) to form the GNS-W composite. The GNS-W composite was characterized by X-ray diffraction (XRD), Raman spectrum, scanning electron microscopy (SEM), transmission electron microscopy (TEM) and X-ray photoelectron spectroscopy (XPS). The as-prepared GNS-W composite was directly fabricated into a supercapacitor electrode for potential energy storage application, and electrochemically tested by cyclic voltammetry, galvanostatic charge/discharge and electrochemical impedance spectroscopy. The GNS-W composite electrode exhibits a better electrochemical performance than that of the WO3/WO3·H2O mixtures electrode. A high specific capacitance of about 143.6 F g−1 at a current density of 0.1 A g−1 for the GNS-W composite delivers significant improvement than that for the WO3/WO3·H2O mixtures and GNS electrodes. The impedance studies also suggest that the GNS-W composite electrode shows the lower resistance and high conductivity due to the good interaction between the graphene nanosheets and the WO3/WO3·H2O mixtures. The good electrochemical performance for the GNS-W composite may be attributed to the interaction between the WO3/WO3·H2O mixtures and the edges of graphene nanosheets, which increases the ion diffusion rate as well as the conductivity.
    Ceramics International 04/2014; 40(3):4109–4116. DOI:10.1016/j.ceramint.2013.08.065 · 2.09 Impact Factor
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    ABSTRACT: The synthesis as well as the electrochemical properties study of highly crystalline ZnCo2O4 powders is presented. ZnCo2O4 powders with a particle diameter of 15-35 nm have been successfully prepared with the surfactant-mediated method. The thorough structural characterization including X-ray diffraction (XRD), scanning electron microscopy (SEM), and transmission electron microscopy (TEM) were employed to examine the morphology and the microstructure of the final product. The as-synthesized powders were used as anode materials for lithium-ion battery, whose charge-discharge properties, cyclic voltammetry, and cycle performance were examined and revealed very good properties. Galvanostatic cycling of ZnCo2O4 powders in the voltage range 0.005-3.0 V versus Li at 60 mA g−1 maintained charge and discharge capacities of 1,308 and 1,336 mAh g−1 after 40 cycles when cycled at 25 °C, respectively.
    Ionics 03/2014; 21(3). DOI:10.1007/s11581-014-1221-1 · 1.84 Impact Factor
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    ABSTRACT: A new visible-light sensitive photocatalyst, Ni(OH)2 nanosheets/ZnO nanorods composites with different Ni(OH)2 contents, was synthesized via a facile water bath route. X-ray diffraction (XRD), transmission electron microscopy (TEM) and UV–vis spectroscopy were used to examine the morphology and microstructure of the composites. XRD analysis revealed that the two phases were composed of well crystallized ZnO and poorly crystallized Ni(OH)2·0.75H2O. Ni(OH)2/ZnO composites showed a red shift in band edge absorption peak in the UV–vis absorbance spectrum, which enhanced their photocatalytic activity. The composites were used for the photodecolourization of water solution of representative organic azo dyes (rhodamine B (RB), Congo red (CR), methylene blue (MB) and methyl orange (MO)) under visible light. All synthesized composites showed better photodegradation performance compared to either pure ZnO or Ni(OH)2. It was noted that composites removal rate strongly depended on the Ni(OH)2 content. When Ni(OH)2 fraction was 10 wt% composites exhibited maximum photodegradation performance for all organic azo dyes. Finally, the possible formation mechanism of the Ni(OH)2/ZnO composites and their photocatalytic decomposition mechanism were also proposed and discussed.
    Ceramics International 01/2014; 40(1):57–65. DOI:10.1016/j.ceramint.2013.05.103 · 2.09 Impact Factor
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    ABSTRACT: The present study seeks to investigate the role of cathepsin L in glutamate receptor-induced transcription factor nuclear factor-kappa B (NF-κB) activation and excitotoxicity in rats striatal neurons. Stereotaxic administration of the N-methyl-d-aspartate (NMDA) receptor agonist Quinolinic acid (QA) into the unilateral striatum was used to produce the in vivo excitotoxic model. Co-administration of QA and the cathepsin L inhibitor Z-FF-FMK or 1-Naphthalenesulfonyl-IW-CHO (NaphthaCHO) was used to assess the contribution of cathepsin L to QA-induced striatal neuron death. Western blot analysis and cathepsin L activity assay were used to assess the changes in the levels of cathepsin L after QA treatment. Western blot analysis was used to assess the changes in the protein levels of inhibitor of NF-κB alpha isoform (IκB-α) and phospho-IκB alpha (p-IκBα) after QA treatment. Immunohistochemical analysis was used to detect the effects of Z-FF-FMK or NaphthaCHO on QA-induced NF-κB. Western blot analysis was used to detect the effects of Z-FF-FMK or NaphthaCHO on QA-induced IκB-α phosphorylation and degradation, changes in the levels of IKKα, p-IKKα, TP53, caspase-3, beclin1, p62, and LC3II/LC3I. The results show that QA-induced loss of striatal neurons were strongly inhibited by Z-FF-FMK or NaphthaCHO. QA-induced degradation of IκB-α, NF-κB nuclear translocation, up-regulation of NF-κB responsive gene TP53, and activation of caspase-3 was strongly inhibited by Z-FF-FMK or NaphthaCHO. QA-induced increases in beclin 1, LC3II/LC3I, and down-regulation of p62 were reduced by Z-FF-FMK or NaphthaCHO. These results suggest that cathepsin L is involved in glutamate receptor-induced NF-κB activation. Cathepsin L inhibitors have neuroprotective effects by inhibiting glutamate receptor-induced IκB-α degradation and NF-κB activation.
    PLoS ONE 09/2013; 8(9):e75702. DOI:10.1371/journal.pone.0075702 · 3.53 Impact Factor
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    ABSTRACT: Resistance exercise training (RET) remains the most effective treatment for the loss of muscle mass and strength in elderly people. However, the underlying cellular and molecular mechanisms are not well understood. Recent evidence suggests that autophagic signaling is altered in aged skeletal muscles. This study aimed to investigate if RET affects IGF-1 and its receptors, the Akt/mTOR, and Akt/FOXO3a signaling pathways and regulates autophagy and apoptosis in the gastrocnemius muscles of 18–20 month old rats. The results showed that 9 weeks of RET prevented the loss of muscle mass and improved muscle strength, accompanied by reduced LC3-II/LC3-I ratio, reduced p62 protein levels, and increased levels of autophagy regulatory proteins, including Beclin 1, Atg5/12, Atg7, and the lysosomal enzyme cathepsin L. RET also reduced cytochrome c level in the cytosol but increased its level in mitochondrial fraction, and inhibited cleaved caspase 3 production and apoptosis. Furthermore, RET upregulated the expression of IGF-1 and its receptors but downregulated the phosphorylation of Akt and mTOR. In addition, RET upregulated the expression of total AMPK, phosphorylated AMPK, and FOXO3a. Taken together, these results suggest that the benefits of RET are associated with increased autophagy activity and reduced apoptosis of muscle cells by modulating IGF-1 and its receptors, the Akt/mTOR and Akt/FOXO3a signaling pathways in aged skeletal muscles.
    Experimental gerontology 04/2013; 48(4):427–436. DOI:10.1016/j.exger.2013.02.009 · 3.53 Impact Factor
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    Yan Wang, Zheng-Hong Qin
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    ABSTRACT: The cell biological phenomenon of autophagy has attracted increasing attention in recent years, partly as a consequence of the discovery of key components of its cellular machinery. Autophagy plays a crucial role in a myriad of cellular functions. Autophagy has its own regulatory mechanisms, but this process is not isolated. Autophagy is coordinated with other cellular activities to maintain cell homeostasis. Autophagy is critical for a range of human physiological processes. The multifunctional roles of autophagy are explained by its ability to interact with several key components of various cell pathways. In this review, we focus on the coordination between autophagy and other physiological processes, including the ubiquitin-proteasome system (UPS), energy homeostasis, aging, programmed cell death, the immune responses, microbial invasion and inflammation. The insights gained from investigating autophagic networks should increase our understanding of their roles in human diseases and their potential as targets for therapeutic intervention.
    Acta Pharmacologica Sinica 03/2013; 34(5). DOI:10.1038/aps.2012.194 · 2.50 Impact Factor
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    ABSTRACT: Aim:To investigate the antitumor actions of the Crotalus durissus neurotoxin (crotoxin) on human esophageal carcinoma (Eca-109) cells in vitro and transplanted esophageal Eca-109 tumors in nude mice.Methods:The growth-inhibitory effect was analyzed in Eca-109 cells using MTT assay. Cell morphology changes in nuclei were observed using Hoechst 33342 staining, while apoptosis and cell cycle distribution were examined by flow cytometry. RT-PCR was used to measure the Bcl-2, p15, and caspase-3 p17 gene expression levels. A tumor transplantation model was established by inoculation of Eca-109 cells were into female Balb/c nude mice. Crotoxin (25, 50, and 100 mg/kg) was subcutaneously injected into the transplanted tumors every 2 d for a total of 10 injections. Tumor size and weight were measured. Bcl-2, p15, and caspase-3 p17 protein expression in transplanted tumors was analyzed using Western blotting.Results:Crotoxin (25, 50, and 100 μg/mL) inhibited the growth of Eca-109 cells in a dose-dependent manner with inhibition rates of 22.9%, 35.8%, and 57.2%, respectively. Hoechst 33342 staining revealed apoptotic cells with pyknotic nuclear chromatin after crotoxin treatment. In Eca-109 cells, crotoxin induced apoptosis and G1 block, significantly upregulated the expression of p15 and caspase-3 p17 genes and downregulated the expression of Bcl-2 gene. Furthermore, crotoxin inhibited the growth of Eca-109 tumors in nude mice in a dose-dependent manner. Western blotting showed that crotoxin increased p15 and caspase-3 p17 protein levels and reduced Bcl-2 protein level in tumor specimens.Conclusion:Crotoxin inhibits the growth of Eca-109 cells in vitro via apoptosis induction and G1 block. Local administration of crotoxin inhibits the growth of subcutaneously transplanted Eca-109 cells in nude mice, possibly via increasing p15 and caspase-3 p17 protein expression and reducing Bcl-2 protein expression.
    Acta Pharmacologica Sinica 12/2012; 34(2). DOI:10.1038/aps.2012.156 · 2.50 Impact Factor
  • Jie Xue, Yan Wang, Rong Han
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    ABSTRACT: To investigate the distributive rule of Chinese medicine syndromes in patients with hyperlipidemia (HLE) in Xinjiang region. Chinese medicine syndrome of HLE patients were differentiated. The common factors of diagnostic information for dominating HLE were extracted by factor analysis and the syndrome type was determined based on Chinese medicine theory and experiences of experts. The syndromes in HLE patients of Xinjiang region were mainly the Pi-Shen deficiency with insufficient Jin syndrome (I), the qi-blood deficiency syndrome (II), the Fei-Wei dryness syndrome (III), the phlegm-dampness obstructing Fei syndrome (IV), the dampness-heat accumulation syndrome (V), and the Gan-Shen yin-deficiency with blood-stasis syndrome (VI). Syndrome II was the most commonly encountered one (35.09%), the next were syndrome III (29.82%) and I (13.45%). Scores of the 6 syndromes, which embodied the severity of disease, were different significantly (F = 32.746, P < 0.01), the highest presented in syndrome I and the second in Syndrome VI. Syndrome in hyperlipidemia patients of Xinjiang region is dominantly the deficiency syndrome, combined with qi-stagnancy and blood-stasis, showing a complex state involving multiple organs and all the qi, blood and Jin-Ye in body.
    Zhongguo Zhong xi yi jie he za zhi Zhongguo Zhongxiyi jiehe zazhi = Chinese journal of integrated traditional and Western medicine / Zhongguo Zhong xi yi jie he xue hui, Zhongguo Zhong yi yan jiu yuan zhu ban 11/2010; 30(11):1169-72.
  • Yan Wang, Zheng-Hong Qin
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    ABSTRACT: Glutamate receptor-mediated excitatory neurotransmission plays a key role in neural development, differentiation and synaptic plasticity. However, excessive stimulation of glutamate receptors induces neurotoxicity, a process that has been defined as excitotoxicity. Excitotoxicity is considered to be a major mechanism of cell death in a number of central nervous system diseases including stroke, brain trauma, epilepsy and chronic neurodegenerative disorders. Unfortunately clinical trials with glutamate receptor antagonists, that would logically prevent the effects of excessive receptor activation, have been associated with untoward side effects or little clinical benefit. Therefore, uncovering molecular pathways involved in excitotoxic neuronal death is of critical importance to future development of clinical treatment of many neurodegenerative disorders where excitotoxicity has been implicated. This review discusses the current understanding of the molecular and cellular mechanisms of excitotoxicity and their roles in the pathogenesis of diseases of the central nervous system.
    Apoptosis 03/2010; 15(11):1382-402. DOI:10.1007/s10495-010-0481-0 · 3.61 Impact Factor
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    ABSTRACT: The present study sought to investigate mechanisms by which p53 induction contributes to excitotoxic neuronal injury. Rats were intrastriatally administered the N-methyl-D-aspartate (NMDA) receptor agonist quinolinic acid (QA), the changes in the expression of p53 and its target genes involved in apoptosis and autophagy, including p53-upregulated modulator of apoptosis (PUMA), Bax, Bcl-2, damage-regulated autophagy modulator (DRAM) and other autophagic proteins including microtubule-associated protein 1 light chain 3 (LC3) and beclin 1 were assessed. The contribution of p53-mediated autophagy activation to apoptotic death of striatal neurons was assessed with co-administration of the nuclear factor-kappaB (NF-kappaB) inhibitor SN50, the p53 inhibitor Pifithrin-alpha (PFT-alpha) or the autophagy inhibitor 3-methyladenine (3-MA). The increased formation of autophagosomes and secondary lysosomes were observed with transmission electron microscope after excitotoxin exposure. QA induced increases in the expression of p53, PUMA, Bax and a decrease in Bcl-2. These changes were significantly attenuated by pre-treatment with SN50, PFT-alpha or 3-MA. SN50, PFT-alpha or 3-MA also reversed QA-induced upregulation of DRAM, the ratio of LC3-II/LC3-I and beclin 1 protein levels in the striatum. QA-induced internucleosomal DNA fragmentation and loss of striatal neurons were robustly inhibited by SN50, PFT-alpha or 3-MA. These results suggest that overstimulation of NMDA receptors can induce NF-kappaB-dependent expression of p53. p53 participates in excitotoxic neuronal death probably through both apoptotic and autophagic mechanisms.
    European Journal of Neuroscience 12/2009; 30(12):2258-2270. DOI:10.1111/j.1460-9568.2009.07025.x · 3.67 Impact Factor
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    ABSTRACT: Actin cytoskeletal remodeling is essential for neurite outgrowth. LIM kinase 1 (LIMK1) regulates actin cytoskeletal remodeling by phosphorylating and inactivating cofilin, an actin filament-disassembling factor. In this study, we investigated the role of LIMK1 in calcium signal-induced neurite outgrowth. The calcium ionophore ionomycin induced LIMK1 activation and cofilin phosphorylation in Neuro-2a neuroblastoma cells. Knockdown of LIMK1 or expression of a kinase-dead mutant of LIMK1 suppressed ionomycin-induced cofilin phosphorylation and neurite outgrowth in Neuro-2a cells. Ionomycin-induced cofilin phosphorylation and neurite outgrowth were also blocked by KN-93, an inhibitor of Ca(2+)/calmodulin-dependent protein kinases (CaMKs), and STO-609, an inhibitor of CaMK kinase. An active form of CaMKIV but not CaMKI enhanced Thr-508 phosphorylation of LIMK1 and increased the kinase activity of LIMK1. Moreover, the active form of CaMKIV induced cofilin phosphorylation and neurite outgrowth, and a dominant negative form of CaMKIV suppressed ionomycin-induced neurite outgrowth. Taken together, our results suggest that LIMK1-mediated cofilin phosphorylation is critical for ionomycin-induced neurite outgrowth and that CaMKIV mediates ionomycin-induced LIMK1 activation.
    Journal of Biological Chemistry 09/2009; 284(42):28554-62. DOI:10.1074/jbc.M109.006296 · 4.60 Impact Factor

Publication Stats

649 Citations
102.32 Total Impact Points


  • 2014–2015
    • Yunnan University
      Yün-nan, Yunnan, China
  • 2006–2012
    • Soochow University (PRC)
      • Department of Pharmacology
      Wu-hsien, Jiangsu Sheng, China
  • 2009
    • Jilin University
      Yung-chi, Jilin Sheng, China
  • 2005
    • Tohoku University
      • Department of Biomolecular Sciences
      Miyagi, Japan
  • 2004
    • Akita University
      Akita, Akita, Japan