Junping Tian

Publications (2)6.91 Total impact

• Article: A graphene and multienzyme functionalized carbon nanosphere-based electrochemical immunosensor for microcystin-LR detection.

  Huimin Zhao, Junping Tian, Xie Quan
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  ABSTRACT: A graphene-based immunosensor was fabricated for the detection of microcystin-LR using a horseradish peroxidase-carbon nanosphere-antibody system for signal amplification. Graphene and chitosan were used as immobilization materials to improve the electrochemical performance of the electrode. Signal amplification was achieved using multienzyme functionalized carbon nanosphere. This approach provided a linear range from 0.05 to 15μgL(-1) microcystin-LR with a detection limit of 0.016μgL(-1). Analytical detection of microcystin-LR in environmental water samples were demonstrated by comparing the results obtained using immunosensor and high-performance liquid chromatography. This electrochemical immunosensor showed good performance with high sensitivity, acceptable stability, repeatability and the potential for use in routine water quality monitoring for various toxins.
  Colloids and surfaces. B, Biointerfaces 10/2012; 103C:38-44. · 2.60 Impact Factor
• Article: Detection of influenza A virus based on fluorescence resonance energy transfer from quantum dots to carbon nanotubes.

  Junping Tian, Huimin Zhao, Meng Liu, Yaqiong Chen, Xie Quan
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  ABSTRACT: In this paper, a simple and sensitive approach for H5N1 DNA detection was described based on the fluorescence resonance energy transfer (FRET) from quantum dots (QDs) to carbon nanotubes (CNTs) in a QDs-ssDNA/oxCNTs system, in which the QDs (CdTe) modified with ssDNA were used as donors. In the initial stage, with the strong interaction between ssDNA and oxCNTs, QDs fluorescence was effectively quenched. Upon the recognition of the target, the effective competitive bindings of it to QDs-ssDNA occurred, which decreased the interactions between the QDs-ssDNA and oxCNTs, leading to the recovery of the QDs fluorescence. The recovered fluorescence of QDs was linearly proportional to the concentration of the target in the range of 0.01-20 μM with a detection limit of 9.39 nM. Moreover, even a single-base mismatched target with the same concentration of target DNA can only recover a limited low fluorescence of QDs, illustrating the good anti-interference performance of this QDs-ssDNA/oxCNTs system. This FRET platform in the QDs-ssDNA/oxCNTs system was facilitated to the simple, sensitive and quantitative detection of virus nucleic acids and could have a wide range of applications in molecular diagnosis.
  Analytica chimica acta 04/2012; 723:83-7. · 4.31 Impact Factor