[Show abstract][Hide abstract] ABSTRACT: The aim of the work was the synthesis of gold nanoparticles (GNP) of different sizes and the estimation of their biological activity in vitro and in vivo. Materials and Methods: Water dispersions of gold nanoparticles of different sizes have been synthesized by Davis method and characterized by laser-correlation spectroscopy and transmission electron microscopy methods. The GNP interaction with tumor cells has been visualized by confocal microscopy method. The enzyme activity was determined by standard biochemical methods. GNP distribution and content in organs and tissues have been determined via atomic-absorption spectrometry method; genotoxic influence has been estimated by "Comet-assay" method. Results: The GNP size-dependent accumulation in cultured U937 tumor cells and their ability to modulate U937 cell membrane Na(+),K(+)-АТР-ase activity value has been revealed in vitro. Using in vivo model of Guerin carcinoma it has been shown that GNP possess high affinity to tumor cells. Conclusions: Our results indicate the perspectives of use of the synthesized GNP water dispersions for cancer diagnostics and treatment. It's necessary to take into account a size-dependent biosafety level of nanoparticles.
[Show abstract][Hide abstract] ABSTRACT: Aim. To study a possibility of usage of the alkaline gel electrophoresis method (Comet assay) for determination of genotoxic properties of veterinary vaccines preparations. Methods. The alkaline gel electrophoresis of isolated eukaryotic cells has been used with further visualization of the samples by fluorescent microscopy. Results. Veterinary vaccines testing by the Comet assay method under alkaline conditions revealed that the samples No.1 and No 12 from twelve investigated vaccine preparations had genotoxic influence on eukaryotic cells of CHO-K1 and Vero test cultures, No. 12 sample being genotoxic only at metabolic activation. Conclusions. The method of alkaline gel ålectrophoresis of single cells (Comet assay) is suitable for determination of veterinary vaccines genotoxic influence on the test eukaryotic cells CHO-K1. The method proposed in this paper is express, inexpensive and predictive. The accomplished experimental work allows us to recommend this method for characterization of biosafety of veterinary vaccines preparations.