Nakarin Suwannarach

Chiang Mai University, Amphoe Muang Chiang Mai, Chiang Mai, Thailand

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Publications (15)18.08 Total impact

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    ABSTRACT: Muscodor cinnamomi was selected and investigated for its in vitro ability to produce indole-3-acetic acid (IAA) to solubilize different toxic metal (Ca, Co, Cd, Cu, Pb, Zn)-containing insoluble minerals and tolerance to metals, herbicides and an insecticide. The results indicated that this fungus is able to produce IAA (45.36±2.40 μg ml-1) in liquid media. This phytohormone stimulated coleoptile elongation, and increased seed germination and root elongation of tested plants. The metal tolerance and solubilizing ability depended on the type of insoluble minerals. Muscodor cinnamomi showed the highest growth tolerance on Ca-containing media at 150 mM, followed by Zn-containing media at 100 mM and Cd-containing media at 10 mM. This fungus tolerated the three herbicides (2,4-d-dimethylammonium, glyphosate and paraquat dichoride) and an insecticide (methomyl) at the recommended dosages for field application. Moreover, M. cinnamomi completely controlled Rhizoctonia solani AG-2 root rot in tomato plants, and increased root length, shoot dry weight and root dry weight. This is the first report of in vitro IAA production, solubilization of insoluble metal minerals, and tolerance to herbicides, an insecticide and metals as well as the plant growth promoting ability of M. cinnamomi.
    Biological Control 05/2015; 90. DOI:10.1016/j.biocontrol.2015.05.008
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    ABSTRACT: Dicitrinin E (1), a new citrinin dimer, together with the known metabolites, dicitrinin A (2), citrinin (3), and fumitremorgin C (4), were isolated from the broth culture of Aspergillus terreus strain ZDF21. The structure of dicitrinin E (1) was elucidated through detailed analysis of 1D and 2D NMR experiments, CD and mass spectra The cytotoxicity of 1 was tested against larvae of Artemia salina.
    Natural product communications 04/2015; 10(4):623-4.
  • Nakarin Suwannarach, Jaturong Kumla, Saisamorn Lumyong
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    ABSTRACT: A leaf blight on oil palm, caused by Alternaria longipes, was found in an Elaeis guineensis plantation for the first time in Chiang Mai Province, Thailand. The fungus was isolated from the lesions and its pathogenicity was confirmed. The fungus was identified based on morphological characteristics and confirmed using comparisons of DNA sequences of internal transcribed spacer (ITS) regions 1 and 2 plus 5.8S rDNA. This report is the first on oil palm leaf blight disease caused by A. longipes.
    Phytoparasitica 02/2015; 43(1). DOI:10.1007/s12600-014-0429-y
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    ABSTRACT: The present study investigated both in vitro and in vivo biofumigant ability of the endophytic fungus, Muscodor suthepensis CMU-Cib462 to control Penicillium digitatum, the main cause of tangerine fruit rot. Volatile compounds from Muscodor suthepensis inhibited mycelial growth of the pathogen. The most abundant compound was 2-Methylpropanoic acid, followed by 3-Methylbutan-1-ol. They showed median effective doses (ED50 ) on P. digitatum growth of 74.91±0.73 and 250.29±0.29 μL L(-1) of airspace, respectively. Rye grain was a suitable solid medium for M. suthepensis inocula production. The result indicated that mycofumigation with 30 g rye grain culture of M. suthepensis for 12 h controlled tangerine fruit rot. The percent weight loss and soluble solids concentrations in fumigated tangerines were similar to the non-infected and non-fumigated treatment. Muscodor suthepensis has potential as a biofumigant for controlling postharvest disease of tangerine fruit. This article is protected by copyright. All rights reserved.
    Journal of the Science of Food and Agriculture 01/2015; DOI:10.1002/jsfa.7099
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    ABSTRACT: Morganella purpurascens previously known from Asia, Australia and Pacific Islands, is reported here for the first time from Chiang Mai Province, northern Thailand. This species has tuberculated exoperidium, pitted endoperidium, paracapillitium abundant and minutely spiny basidiospores. A molecular phylogenetic analysis confirmed that this species is most closely related to known Morganella species and is positioned within Lycoperdon sensu lato. A description and illustration are provided.
    Mycoscience 01/2014; 55(1):49–52. DOI:10.1016/j.myc.2013.05.002
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    ABSTRACT: The genus Muscodor comprises fungal endophytes which produce mixtures of volatile compounds (VOCs) with antimicrobial activities. In the present study, four novel species, Muscodor musae, M. oryzae, M. suthepensis and M. equiseti were isolated from Musa acuminata, Oryza rufipogon, Cinnamomum bejolghota and Equisetum debile, respectively; these are medicinal plants of northern Thailand. The new Muscodor species are distinguished based on morphological and physiological characteristics and on molecular analysis of ITS-rDNA. Volatile compound analysis showed that 2-methylpropanoic acid was the main VOCs produced by M. musae, M. suthepensis and M. equiseti. The mixed volatiles from each fungus showed in vitro antimicrobial activity. Muscodor suthepensis had the highest antifungal activity.
    Annals of Microbiology 12/2013; 63(4). DOI:10.1007/s13213-012-0593-6
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    ABSTRACT: A leaf spot on oil palm, caused by Pestalotiopsis theae, was found in a plantation of Elaeis guineensis for the first time in the world in Chiang Mai Province, Thailand. The fungus was isolated from lesions on leaves, and its pathogenicity was confirmed. Pathogenicity tests showed that P. theae could infect E. guineensis, which developed the same symptoms after inoculation as those observed naturally in the field. The fungus was identified based on morphological characteristics and confirmed using comparisons of DNA sequences of internal transcribed spacer (ITS)1, ITS2 and 5.8S rDNA. This report is the first on oil palm leaf spot disease caused by P. theae.
    Journal of General Plant Pathology 07/2013; 79(4). DOI:10.1007/s10327-013-0453-7
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    ABSTRACT: Sclerodermatoid fungi basidiomes were collected from northern Thailand and pure cultures were isolated. The morphology and molecular characteristics identified them as Astraeus odoratus, Phlebopus portentosus, Pisolithus albus and Scleroderma sinnamariense. This study investigated the in vitro ability of selected fungi to produce indole-3-acetic acid (IAA), to solubilize different toxic metal (Co, Cd, Cu, Pb, Zn)-containing minerals, and metal tolerance. The results indicated that all fungi are able to produce IAA in liquid medium. The optimum temperature for IAA production of all fungi was 30 °C, and the optimum concentration of L-tryptophan of Astraeus odoratus, Pisolithus albus and Scleroderma sinnamariense was 2 mg ml−1. The highest IAA yield (65.29 ± 1.17 μg ml−1) was obtained from Phlebopus portentosus after 40 days of cultivation in culture medium supplemented with 4 mg ml−1 of L-tryptophan. The biological activity tests of fungal IAA showed that it can simulate coleoptile elongation, and increase seed germination and root length of tested plants. In addition, the metal tolerance and solubilizing activities varied for different minerals and fungal species. The presence of metal minerals affected fungal growth, and cobalt carbonate showed the highest toxicity. The solubilization index decreased when the concentration of metal minerals increased. Astraeus odoratus showed the lowest tolerance to metals. This is the first report of in vitro IAA production, solubilization of insoluble metal minerals and metal tolerance abilities of the tested fungi.
    Annals of Microbiology 06/2013; 64(2):707-720. DOI:10.1007/s13213-013-0706-x
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    ABSTRACT: Forty-six fungal endophytes were isolated from Lagerstroemia loudoni. Only one fungus, identified as Nodulisporium spp. CMU-UPE34, produced antifungal volatile compounds. It produced 31 volatile compounds, primarily composed of alcohols, acids, esters and monoterpene. The most abundant volatile compound was eucalyptol. In vitro tests showed that volatile compounds produced by Nodulisporium spp. CMU-UPE34 inhibited or killed 12 different plant pathogens. In vivo mycofumigation with jasmine rice grain cultures of Nodulisporium spp. CMU-UPE34 controlled green mold decay on Citrus limon caused by Penicillium digitatum, blue mold decay of Citrus aurantifolia and Citrus reticulata caused by Penicillium expansum. Nodulisporium spp. CMU-UPE34 has potential as a biofumigant for controlling postharvest disease.
    Crop Protection 03/2013; 45:63–70. DOI:10.1016/j.cropro.2012.11.015
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    ABSTRACT: A new species of earthball, described herein as Scleroderma suthepense, was found under Prunus cerasoides in Doi Suthep-Pui National Park, Thailand. Morphological characteristics indicate it belongs to section Scleroderma. Molecular analysis showed differences from previously described Scleroderma species. A description, illustration, and comparisons with related taxa are provided.
    Mycotaxon -Ithaca Ny- 01/2013; 123:1-7. DOI:10.5248/123.1
  • Thet Thet Mar, Nakarin Suwannarach, Saisamorn Lumyong
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    ABSTRACT: Spore productivity in six entomopathogenic fungal strains isolated from insect cadavers at four locations in Chiang Mai province was evaluated in five cereal grains: white-rice, wheat, rye, corn and sorghum. According to sequence analysis of the internal transcribed spacer regions of these isolates, they were closely related to Beauveria bassiana (2 isolates), Metarhizium flavoviride (1 isolate), Metarhizium anisopliae (1 isolate), Paecilomyces lilacinus (1 isolate) and Isaria tenuipes (1 isolate). Among all fungal isolates, the maximum amount of spores (530.0 × 10(9) conidia/g) was yielded P. lilacinus CMUCDMT02 on sorghum grain followed by white-rice (399.3 × 10(9) conidia/g). Moreover, the highest number of spore in M. flavoviride was 102.8 × 10(9) conidia/g sorghum whereas white-rice yielded the greatest amount of spore for B. bassiana CMUCDMF03 (141.0 × 10(9) conidia/g) after 60 days incubation. The fungal growth rate was found highest in corn for all strains and rye showed the lowest with the exception of P. lilacinus CMUCDMT02 among the tested grains. Spore viability was over 80 % for all isolates that had been inoculated for 60 days. Fungal conidia suspension of P. lilacinus obtained highest virulence against Bactrocera spp. at a concentration of 1 × 10(6) spore/ml. The strains isolated, exhibited good production of conidia suggesting a promising strategy for the mass production of inoculum as biocontrol agents with low production cost.
    World Journal of Microbiology and Biotechnology (Formerly MIRCEN Journal of Applied Microbiology and Biotechnology) 09/2012; 28(12). DOI:10.1007/s11274-012-1139-6
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    ABSTRACT: Rhizoctonia solani is a damping-off pathogen that causes significant crop loss worldwide. In this study, the potential of Muscodor cinnamomi, a new species of endophytic fungus for controlling R. solani AG-2 damping-off disease of plant seedlings by biological fumigation was investigated. In vitro tests showed that M. cinnamomi volatile compounds inhibited mycelial growth of pathogens. Among nine solid media tested, rye grain was the best grain for inoculum production. An in vivo experiment of four seedlings, bird pepper, bush bean, garden pea and tomato were conducted. The results indicated that treatment with 30 g of M. cinnamomi inoculum was the minimum dose that caused complete control of damping-off symptoms of all seedlings after one month of planting. The R. solani-infested soil showed the lowest percentage of seed germination. In addition, M. cinnamomi did not cause any disease symptoms. From the results it is clear that M. cinnamomi is effective in controlling R. solani AG-2 both in vitro and in vivo.
    World Journal of Microbiology and Biotechnology (Formerly MIRCEN Journal of Applied Microbiology and Biotechnology) 07/2012; 28(11):3171-7. DOI:10.1007/s11274-012-1127-x
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    ABSTRACT: A leaf blight on eucalyptus, caused by Pestalotiopsis virgatula, was found on a Eucalyptus camaldulensis plantation for the first time at Chiang Mai Province, Thailand in 2011. The fungus was isolated and its pathogenicity confirmed. Pathogenicity tests showed that P. virgatula could infect E. camaldulensis, which developed the same symptoms under artificial inoculation conditions as those observed in the field. The fungus was identified based on morphological and culture characteristics as P. virgatula. Identifications were confirmed using comparisons of DNA sequences of internal transcribed spacers (ITS) regions 1 and 2, including 5.8S rDNA (ITS1–5.8S–ITS2). This is the first report of eucalyptus leaf blight disease caused by P. virgatula.
    Canadian Journal of Plant Pathology 04/2012; DOI:10.1080/07060661.2012.680501
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    ABSTRACT: Phlebopus portentosus is a popular wild edible ectomycorrhizal fungus in northern Thailand. In general ectomycorrhizal fungi produce basidiomes when associated with a host plant. In this paper mycelium growth and basidiome production of P. portentosus were examined in pure culture both in vitro and in pot-culture experiments. Five mycelial strains of P. portentosus were isolated from basidiomes and used in the experiments. The mycelia grew fastest on sorghum grains supplemented with fungal-host solution. The mycelia produced sclerotia-like structures after 3 wk incubation in darkness at 30 C. All strains of P. portentosus had the ability to form primordia. The primordia were formed under lowered temperature, high humidity and a 12 h photo-period. They developed to mature basidiomes after 8-12 d in in vitro. In the pot-culture primordia were found after 28-35 d incubation in the greenhouse and mature basidiomes released basidiospores within 6-8 d. Basidiospores were germinated on fungal-host medium and formed mycelial colonies. This fungus showed an ability to produce basidiomes even 2 y after the original isolation from tissues. This research provides valuable information concerning the techniques and protocols for the large scale commercial production of P. portentosus basidiomes in the absence of a host plant.
    Mycologia 03/2012; 104(3):597-603. DOI:10.3852/11-074
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    ABSTRACT: Muscodor cinnamomi is described as a new species, endophytic within leaf tissues of Cinnamomum bejolghota (Lauraceae) in Doi Suthep-Pui National Park, Northern Thailand. Molecular analysis indicated differences from the five previously described Muscodor spp. Volatile organic compounds analysis showed that M. cinnamomi produced azulene (differentiating it from M. crispans) but did not produce naphthalene (differentiating it from M. albus, M. roseus, and M. vitigenus).
    Mycotaxon -Ithaca Ny- 09/2010; 114:15-23. DOI:10.5248/114.15