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ABSTRACT: A Gram-staining-negative, rod-shaped and non-spore-forming bacterium, designated strain R27T, was isolated from Grimmia montana collected from Beijing Songshan National Nature Reserve, China and characterized by using a polyphasic taxonomic approach. The predominant fatty acids of strain R27T were C18:1ω7c (31.4 %), C16:0 (16.3 %), summed feature 3 (C16:1ω7c and/or C16:1 ω6c , 15.8 %) and C17:0 cyclo (8.7%); its major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, and three uncharacterized aminolipids and one unknown phospholipid. Strain R27T contained Q-8 as the dominant isoprenoid quinone, and the G+C content of its genomic DNA was 64.6 mol%. On the basis of 16S rRNA gene sequence analysis, strain R27T showed 99.1 % sequence similarity to the closest related species Burkholderia zhejiangensis OP-1T, and 97.6 % sequence similarity to Burkholderia glathei ATCC 29195T. However, the DNA-DNA relatedness values between strain R27T and B. zhejiangensis OP-1T and B. glathei ATCC 29195T were 10.2% and 14.9%, respectively. Based on the 16S rRNA and rpoB gene sequence similarities and phenotypic and chemotaxonomic data, strain R27T is considered to represent a novel species of the genus Burkholderia, for which the name Burkholderia grimmiae sp. nov. is proposed. The type strain is R27T (= CGMCC 1.11013T = DSM 25160T).
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 10/2012; · 2.11 Impact Factor
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ABSTRACT: A Gram-staining-positive, facultatively anaerobic and rod-shaped bacterium, designated strain xj7T, was isolated from roots of Typha angustifolia L. growing in Beijing Cuihu Wetland, China. The isolate was identified as a member of the genus Paenibacillus based on the phenotypic characteristics and phylogenetic inference. The novel strain was spore-forming, motile, catalase-positive and oxidase-negative. Optimal growth of strain xj7T occurred at 28-30 °C and pH 7.0-7.5. Diphosphatidylglycerol was the most abundant polar lipid in comparison to phosphatidylglycerol, phosphatidylethanolamine, one unknown phospholipid and three unknown aminophospholipids. The diamino acid found in the cell-wall peptidoglycan was meso-diaminopimelic acid. The predominant isoprenoid quinone was MK-7. The major fatty acid components were anteiso-C15: 0 (56.1 %), iso-C16:0 (9.1 %), C16:0 (8.0 %), iso-C14:0 (6.3 %) and iso-C15:0 (5.1 %). The G+C content of genomic DNA was 47.9 mol%. Phylogenetic analysis based on the 16S rRNA gene sequence showed that strain xj7T fell within the evolutionary radiation encompassed by the genus Paenibacillus, its closest neighbours were Paenibacillus borealis KK19T (97.5 %) and Paenibacillus durus DSM 1735T (97.1%). However, the DNA-DNA relatedness values between strain xj7T and P. borealis KK19T, and that between strain xj7T and P. durus DSM 1735T, were both 35 %. Based on their phenotypic, chemotaxonomic and phylogenetic properties, strain xj7T is considered to represent a novel species of the genus Paenibacillus, for which the name Paenibacillus typhae sp. nov. is proposed. The type strain is xj7T (= CGMCC 1.11012T = DSM 25190T).
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 06/2012; · 2.11 Impact Factor
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ABSTRACT: A Gram-negative-staining and rod-shaped bacterium, designated strain 333-1-0411T, was isolated from a soil sample collected from Namucuo, Tibet Autonomous Region, China and characterized by using a polyphasic taxonomic approach. A phylogenetic tree based on 16S rRNA gene sequence showed that the strain 333-1-0411T fell within the evolutionary radiation encompassed by the genus Massilia. The 16S rRNA gene sequence similarities between strain 333-1-0411T and the type strains of recognized Massilia species ranged from 95.4-97.2 %, and the most closely related strains were Massilia flava CGMCC 1.10685T (97.2 %) and Massilia albidiflava KCTC 12343T (97.0 %). The predominant ubiquinone of strain 333-1-0411T was Q-8. Major fatty acid components were summed feature 3 (C16:1ω7c and/or C16:1ω6c 60.9 %) and C16:0 (22.2 %). The G+C content of genomic DNA was 66.7 mol%, a value that supports the affiliation of strain 333-1-0411T to the genus Massilia. However, the DNA-DNA relatedness values between strain 333-1-0411T and Massilia flava CGMCC 1.10685T and Massilia albidiflava KCTC 12343T were 20.2% and 27.2%, respectively. Based on the phenotypic, chemotaxonomic and phylogenetic properties, strain 333-1-0411T is considered to represent a novel species of the genus Massilia, for which the name Massilia namucuoensis sp. nov. is proposed. The type strain is 333-1-0411T (= CGMCC 1.11014T = DSM 25159T).
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 03/2012; · 2.11 Impact Factor
