[show abstract][hide abstract] ABSTRACT: PRUNE2 has played an important role in regulating tumor cell differentiation, proliferation and invasiveness in neuroblastoma. Our previous study revealed that the PRUNE2/OBSCN relative expression could distinguish between gastrointestinal stromal tumor and leiomyosarcoma accurately. However, the correlation between PRUNE2 expression and prognosis was poorly understood in leiomyosarcoma patients. In this study, we aim to evaluate the prognostic role of PRUNE2 in leiomyosarcoma. The PRUNE2 protein expression was detected by immunohistochemistry (IHC) in 30 formalin-fixed and paraffin-embedded (FFPE) leiomyosarcoma tissues from MD Anderson Cancer Center (MDACC) and the high expression of PRUNE2 was 36.7% (11/30). To validate the results, another cohort of 45 FFPE leiomyosarcoma tissues from Tianjin Medical University Cancer Institute & Hospital (TMUCIH) was collected and PRUNE2 protein expression was detected by IHC. The results showed that the high expression rate of PRUNE2 protein in TMUCIH samples was 37.8% (17/45) and the elevated PRUNE2 expression was significantly associated with tumor size (P=0.03). Not only the high expression level of PRUNE2 protein was a significant favorable prognostic factor for overall survival in TMUCIH leiomyosarcoma patients (P<0.05), multivariate analysis revealed that the protein expression of PRUNE2 was also an independent prognostic factor. These data suggest that increased PRUNE2 protein expression may serve as a favorable prognostic marker in human leiomyosarcoma.
Ai zheng = Aizheng = Chinese journal of cancer 06/2013;
[show abstract][hide abstract] ABSTRACT: Breast cancer is a disease in which cancer cells form in the tissues of the breast. The present study aimed to explore the effect of the flavonoid compound quercetin on the growth and apoptosis of human breast cancer cells. Varying concentrations (12.5, 25, 50, 100, 200 µM) of quercetin were applied to cultured MCF-7 human breast cancer cells for defined lengths of time. At 50 to 200 µM doses, quercetin significantly inhibited the proliferation of MCF-7 cells assessed by MTT colorimetry, in both dose- and time-dependent manners (P<0.05). The compound also increased apoptosis after 48 h of exposure (P<0.05). Furthermore, following quercetin treatment Bcl-2 expression decreased significantly while Bax expression increased significantly (P<0.05). In brief, quercetin inhibits cell growth and induces apoptosis in MCF-7 human breast cancer cells. The mechanisms behind these effects may stem from the downregulation of Bcl-2 protein expression and upregulation of Bax expression.
Molecular Medicine Reports 03/2012; 5(6):1453-6. · 1.17 Impact Factor