Publications (2)4.35 Total impact
-
Article: The catalytic subunit of cAMP-dependent protein kinase A StPKA-c contributes to conidiation and early invasion in the phytopathogenic fungus Setosphaeria turcica.
[show abstract] [hide abstract]
ABSTRACT: Cyclic adenosine mono phosphate (cAMP)-dependent protein kinase A (PKA) is an important mediator of signal transduction in eukaryotic cells. Thus, identifying its function is necessary to understand the cAMP signaling network. StPKA-c, the PKA catalytic subunit gene in Setosphaeria turcica, was investigated by RNA interference technology. Transformant strains M3, M5, and M9 with diverse StPKA-c silencing efficiency were confirmed by reverse transcription-polymerase chain reaction and northern blot. Compared with the wild-type strain 01-23, the transformant strains exhibited increased growth rate and significantly decreased conidium production. In addition, the ratios of spore germination and appressorium formation and penetration were slightly reduced. Relative to the wild-type strain, the transformants demonstrated different colony color, greatly reduced pathogenicity, and similar HT-toxin activity. Further studies showed that the content of intracellular melanin in the transformants significantly decreased and the transcription of transcriptional factor StMR was down-regulated correspondingly. The transcription and enzyme activity of xylanase was also impaired. Thus, we proposed that StPKA-c was mainly involved in the mycelium growth, conidiation, and pathogenesis of S. turcica. Furthermore, it was positively correlated with the biosyntheses of melanin and xylanase but dispensable for the activity of HT-toxin. © 2013 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.FEMS Microbiology Letters 04/2013; · 2.04 Impact Factor -
Article: StRas2 regulates morphogenesis, conidiation and appressorium development in Setosphaeria turcica.
[show abstract] [hide abstract]
ABSTRACT: The proteins of Ras family are a large group of monomeric GTPases and act as molecular switches transducing extracellular signals into the cell in higher eukaryotes. However, little is known about roles of Ras family in the foliar pathogens. In this research, we cloned the gene named StRas2 encoding Ras in Setosphaeria turcica and investigated its function by RNA interference technology. We found that the growth rate of RNAi transformants named as R1, R2, R3, R4, R5 and R6, in which the StRas2 silencing efficiency fell in turn. With the highest silencing efficiency, the transformant R1 showed anomalistic hyphae morphology, indicating its growth was significantly affected. The transformants with a middle-silencing efficiency, such as R3, R4, displayed a delay when forming appressoria and invasive hyphae. R1 could not form conidia and appressoria. However, the conidial formation in R5 and R6 was significantly reduced, and these two transformants could form appressoria and penetrate the artificial cellophane, only that its invasive hyphae were fascicular and rarely branched. The HT-toxin biological activity of all transformants showed no difference. All results suggested that StRas2 is involved in the morphogenesis, conidiation, and appressorium development and is not related to the biosynthesis of HT-toxin.Microbiological Research 03/2012; 167(8):478-86. · 2.31 Impact Factor
