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ABSTRACT: Luminescence data have often been used to study imperfections and to characterize lattice distortions because the signals are sensitive to changes of structure and composition. Previous studies have included intentionally added probe ions such as rare earth ions to sense distortions in local crystal fields caused by modified structural environments. An under-exploited extension of this approach was to use luminescence to monitor crystalline phase changes. A current overview of this new and powerful technique shows that continuous scanning of the sample temperatures immediately offered at least three types of signatures for phase transitions. Because of high sensitivity, luminescence signals were equally responsive to structural changes from inclusions and nanoparticles. These coupled to the host material via long-range interactions and modified the host signals. Two frequently observed examples that are normally overlooked are from nanoparticle inclusions of water and CO(2.) Examples also indicated that phase transitions were detected in more diverse materials such as superconductors and fullerenes. Finally, luminescence studies have shown that in some crystalline examples, high dose ion implantation of surface layers could induce relaxations and/or structural changes of the entire underlying bulk material. This was an unexpected result and therefore such a possibility has not previously been explored. However, the implications for ion implication are significant and could be far more general than the examples mentioned here. Copyright © 2012 John Wiley & Sons, Ltd.
Luminescence 04/2012; · 1.73 Impact Factor
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ABSTRACT: Two pumilios, pum1 and pum2, were identified in medaka Oryzias latipes. Oryzias latipes pum1 and pum2 are ubiquitous in the adult tissues but with specific expression in the germ cells of gonads, ovary and testis. Pum1 is expressed in the spermatogonia to spermatocytes whilst pum2 presents in spermatocytes of testis only. Oryzias latipes pum1 and pum2 are maternally supplied RNA with ubiquitous expression in the early stages, and embryonic expression of pum1 and pum2 may begin from early gastrula. Both pum1 and pum2 are expressed in the tissues including brain, eye and trunk, and both are expressed in the gonads after hatching. Taken together, Pum1 and Pum2 may play important roles in embryonic and germ cell development of O. latipes.
Journal of Fish Biology 01/2012; 80(1):100-9. · 1.68 Impact Factor
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X Li,
Q Ma,
J Wang,
X Liu,
Y Yang,
H Zhao, Y Wang,
Y Jin,
J Zeng,
J Li,
L Song,
P Li,
X Qian,
C Cao
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ABSTRACT: Galectin-3 (Gal3) has important roles in tumor transformation and metastasis. This study shows that c-Abl and Abl-related gene (Arg) associate with and phosphorylate Gal3. The SH (Src homology)3 domains of c-Abl/Arg bind to a P(80)GPPSGP motif of Gal3, and Tyr79 and Tyr118 are the major tyrosine phosphorylation sites. A consequence of this interaction and phosphorylation is the significant impairment of chaperone-mediated autophagy of Gal3. Cells expressing Gal3 and treated with the c-Abl/Arg inhibitor STI571, Gal3-depleted cells, and Gal3-depleted cells expressing Gal3 phosphorylation mutants all display an increased sensitivity to apoptosis-inducing agents. In addition, tumor cells expressing the phosphorylation mutants show impaired tumorigenicity. These results partially explain the antiapoptotic effect of Abl and Arg. As tumors frequently overexpress Gal3, a c-Abl/Arg-specific inhibitor may potentially be applied along with other antitumor drugs to target the lysosomal degradation of Gal3 in tumor therapy.
Cell death and differentiation 02/2010; 17(8):1277-87. · 8.24 Impact Factor
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ABSTRACT: Rapeseed (Brassica napus) is sensitive to low boron (B) stress and plentiful variation exists in response to B deficiency. One major QTL, BE1, and three minor loci controlling B efficiency in Brassica napus were previously detected. To fine map and clone the B-efficient gene (s), the development of B-efficient NILs in Brassica napus was conducted, combining the identification of B efficiency at seedling stage with genetic background selection using random AFLP markers. The molecular marker assisted background selection proved its optimum and necessary in an early backcrossing generation to select the backcross individuals with high genetic background similarity to accelerate the construction of NILs. Based on B efficiency investigated at seedling stage under the low B conditions, the B-efficient backcross line can produce biomass twice about the B-inefficient parent's and show low B concentration and effective utilization of B under low B condition. Thus, the B efficiency of the B efficient NILs might be attributed to the higher B utilization efficiency or less demand for B.
Genetika 01/2010; 46(1):66-72. · 0.44 Impact Factor
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ABSTRACT: The development of allergic rhinitis is considered to be determined by the interaction between genetic and environmental factors. Surfactant protein D (SP-D) has been proposed to offer protection against allergenic challenge at various levels in allergic responses. The present study aimed to investigate whether polymorphisms within the SFTPD gene (Met11Thr, Ala160Thr, and Ser270Thr) are associated with allergic rhinitis. Genotyping of SFTPD polymorphisms was performed using the pyrosequencing method. The study population comprised 216 patients with allergic rhinitis and 84 normal controls. The frequency of 11Thr/Thr genotype and Thr allele in the patient group was significantly higher than that in the control group after applying Bonferroni corrections (P = 0.007 and P = 0.006, respectively). Our subjects with the 11Thr/Thr genotype are more susceptible to allergic rhinitis. There were no significant differences between the patient group and the control group for frequencies of genotypes and alleles in either Ala160Thr or Ser270Thr single nucleotide polymorphisms (P > 0.05). No significant associations could be detected between any of these three SFTPD gene polymorphisms and the skin prick test response (P > 0.05). Meanwhile, there was a lack of association between the three loci and the levels of serum total immunoglobulin E (P > 0.05). In summary, our results suggest that the Met11Thr polymorphism in SP-D plays a major role in the genetic predisposition to allergic rhinitis in Chinese adult population, whereas the other two SP-D polymorphisms displayed no significant association with allergic rhinitis.
Tissue Antigens 06/2009; 73(6):546-52. · 2.59 Impact Factor
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ABSTRACT: To investigate whether and how much gastric cancer patients after curative resection could benefit from chemotherapy.
Meta-analysis was conducted with all the qualified clinical randomized trials which compared adjuvant chemotherapy with surgery alone. The database includes MEDLINE, EMBase and CBM disc, and the censor data were up to November 2007. Primary outcomes were relative risk (RR) on death and disease-free survival (DFS); secondary outcomes include RR of adverse reactions of the two arms. Sub-group analysis and sensitivity analysis were also performed. All the calculations and statistical tests were done with the RevMan 4.2.8 software.
Finally, 23 trials which included 4919 patients (2441 in the adjuvant chemotherapy arm, 2478 in the observation arm) achieved all the criteria. Among them, 19 studies reported the survival rate at the end of follow-up, 60.6% alive among 2286 patients in the adjuvant chemotherapy arm, 53.4% alive among 2313 patients in the observation arm, with the RR on death of 0.85 (95%CI: 0.80-0.90). Eight studies reported the DFS, and the observation arm had a shorter DFS (RR: 0.88, 95%CI: 0.77-0.99). Grade 3/4 of myelosuppression and GI toxicity occurred more frequently in the treatment arm. Nine studies reported the recurrence rate and suggested that the treatment arm had a lower recurrence rate (RR: 0.78, 95%CI: 0.71 approximately 0.86).
Statistically, adjuvant chemotherapy could improve the survival rate and disease-free survival rate in gastric cancer after curative resection and reduce the relapse rate. However, the clinical benefits of adjuvant chemotherapy still need to be improved. Additionally, post-operative chemotherapy could be tolerated.
European journal of surgical oncology: the journal of the European Society of Surgical Oncology and the British Association of Surgical Oncology 04/2008; 34(11):1208-16. · 2.56 Impact Factor
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D Qiu,
C Morgan,
J Shi,
Y Long,
J Liu,
R Li,
X Zhuang, Y Wang,
X Tan,
E Dietrich, [......],
P Beckett,
F Fraser,
M Trick,
S Barnes,
J Wilmer,
R Schmidt,
J Li,
D Li,
J Meng,
I Bancroft
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ABSTRACT: We have developed a new DH mapping population for oilseed rape, named TNDH, using genetically and phenotypically diverse parental lines. We used the population in the construction of a high stringency genetic linkage map, consisting of 277 loci, for use in quantitative genetic analysis. A proportion of the markers had been used previously in the construction of linkage maps for Brassica species, thus permitting the alignment of maps. The map includes 68 newly developed Sequence Tagged Site (STS) markers targeted to the homologues of defined genes of A. thaliana. The use of these markers permits the alignment of our linkage map with the A. thaliana genome sequence. An additional 74 loci (31 newly developed STS markers and 43 loci defined by SSR and RFLP markers that had previously been used in published linkage maps) were added to the map. These markers increased the resolution of alignment of the newly constructed linkage map with existing Brassica linkage maps and the A. thaliana genome sequence. We conducted field trials with the TNDH population at two sites, and over 2 years, and identified reproducible QTL for seed oil content and erucic acid content. The results provide new insights into the genetic control of seed oil and erucic acid content in oilseed rape, and demonstrate the utility of the linkage map and population.
Theoretical and Applied Genetics 01/2007; 114(1):67-80. · 3.30 Impact Factor
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ABSTRACT: The Rf3 gene restores the pollen fertility disturbed by S male sterile cytoplasm. In order to develop molecular markers tightly linked to Rf3, we used amplified fragment length polymorphism (AFLP) technique with near isogenic lines (NILs) and bulk segregant analysis (BSA). A BC(1)F(1) population from a pair of NILs with different Rf3 locus was constructed and 528 primer combinations was screened. A linkage map was constructed around the Rf3 locus, which was mapped on the distal region of chromosome 2 long arm with the help of SSR marker UMC2184. The closest marker E7P6 was 0.9 cM away from Rf3. Marker E3P1, 2.4 cM from Rf3, and E12M7, 1.8 cM from Rf3, were converted into a codominant CAPS and a dominant SCAR marker, and designated as CAPSE3P1 and SCARE12M7, respectively. These markers are useful for marker-assisted selection and map-based cloning of the Rf3 gene.
Molecular and General Genetics 09/2006; 276(2):162-9. · 2.63 Impact Factor
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Chemical Engineering & Technology 06/2005; 28(6):649 - 654. · 1.60 Impact Factor
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ABSTRACT: It is established that sympathetic neurons can synthesize, transport and store tissue plasminogen activator (t-PA) within axon terminals in the smooth muscle of vessel walls. Moreover, sympathetic excitations (e.g. physical and mental stress) are known to induce an acute release of t-PA into the circulation. However, relatively little is known about the nature and extent of sympathetic nervous system involvement in the release process. We inquired whether a chemical sympathectomy will alter the release of t-PA into the blood, and the intrinsic release of stored t-PA from isolated whole vessel explants. A long-term sympathectomy was induced in adult Sprague-Dawley rats by injection of guanethidine during a 5-week course. The destruction of ganglion neurons and vessel wall axons was verified immunohistochemically. t-PA release was assayed as the free activity in hind limb plasma and explant culture medium. Following sympathectomy: (i) the basal t-PA activity in plasma was 70% less than controls (2.92 +/- 1.96 versus 9.33 +/- 1.72 IU/ml; </= 0.001); (ii) the acute release from isolated vessels induced by bradykinin or phenylephrine was comparably reduced; and (iii) the greatest reductions occurred in densely innervated small vessel explants. The results provide new support for an autonomic regulation of neural t-PA release into the vessel wall matrix and blood of densely innervated thin-walled microvessels.
Blood Coagulation and Fibrinolysis 09/2002; 13(6):471-81. · 1.24 Impact Factor
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ABSTRACT: We investigated the role of neurofilament (NF) proteins in Alzheimer disease (AD) neurofibrillary degeneration. The levels and degree of phosphorylation of NF proteins in AD neocortex were determined by Western blots developed with a panel of phosphorylation-dependent NF antibodies. Levels of all three NF subunits and the degree of phosphorylation of NF-H and NF-M were significantly increased in AD as compared to Huntington disease brains used as control tissue. The increase in the levels of NF-H and NF-M was 1.7- and 1.5-fold (P<0.01) as determined by monoclonal antibody SMI33, and was 1.6-fold (P<0.01) in NF-L using antibody NR4. The phosphorylation of NF-H and NF-M in AD was increased respectively at the SMI31 epitope by 1.6- and 1.9-fold (P<0.05) and at the SMI33 epitope by 2.7- and 1.3-fold (P<0.01 and P<0.05). Essentially similar effects were observed in SY5Y human neuroblastoma cells when treated with okadaic acid, an inhibitor of protein phosphatase (PP)-2A and -1. This is the first biochemical evidence which unambiguously demonstrates the hyperphosphorylation and the accumulation of NF subunits in AD brain, and shows that the inhibition of PP-2A/PP-1 activities can lead to the hyperphosphorylation of NF-H and NF-M subunits.
FEBS Letters 11/2001; 507(1):81-7. · 3.54 Impact Factor
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ABSTRACT: The L-asparaginase (ASN) from Escherichia coli AS1.357 was cloned as a DNA fragment generated using polymerase chain reaction technology and primers derived from conserved regions of published ASN gene sequences. Recombinant plasmid pASN containing ASN gene and expression vector pBV220 was transformed in different E. coli host strains. The activity and expression level of ASN in the engineering strains could reach 228 IU/mL of culture fluid and about 50% of the total soluble cell protein respectively, more than 40-fold the enzyme activity of the wild strain. The recombinant plasmid in E. coli AS1.357 remained stable after 72 h of cultivation and 5 h of heat induction without selective pressure. The ASN gene of E. coli AS1.357 was sequenced and had high homology compared to the reported data.
Applied Biochemistry and Biotechnology 09/2001; 95(2):93-101. · 1.94 Impact Factor
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Journal of Materials Science Letters 08/2001; 20(18):1741-1743.
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ABSTRACT: Peking whites breeding hens were selected. Using true metabolizable energy method (TME) to evaluate the available nutritive value of acorn kernel, while maize and rice were used as control. The results showed that the contents of gross energy (GE), apparent metabolizable energy (AME), true metabolizable energy (TME) and crude protein (CP) in the acorn kernel were 16.53 mg/kg-1, 11.13 mg.kg-1, 11.66 mg.kg-1 and 10.63%, respectively. The apparent availability and true availability of crude protein were 45.55% and 49.83%. The gross content of 17 amino acids, essential amino acids and semiessential amino acids were 9.23% and 4.84%. The true availability of amino acid and the content of true available amino acid were 60.85% and 6.09%. The contents of tannin and hydrocyanic acid were 4.55% and 0.98% in acorn kernel. The available nutritive value of acorn kernel is similar to maize or slightly lower, but slightly higher than that of rice. Acorn kernel is a wild economic plant resource to exploit and utilize but it contains higher tannin and hydrocyanic acid.
Ying yong sheng tai xue bao = The journal of applied ecology / Zhongguo sheng tai xue xue hui, Zhongguo ke xue yuan Shenyang ying yong sheng tai yan jiu suo zhu ban 05/2000; 11(2):196-8.
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ABSTRACT: Five sequential cropping systems including rape/soybean-rice, wheat/watermelon-rice, rape/corn-soybean, wheat-sesame and soybean-sesame, were selected study the boron balance in agroecosystem on brown-red soil of south Hubei Province. The results show that the main output of boron was runoff and leaching, which accounted for 48-53% and 41% of the total respectively, while the output by products accounted for 3.4-10.1%. The main inputs were from rainfall, irrigation, fertilization seeds and seedlings, and natural return.
Ying yong sheng tai xue bao = The journal of applied ecology / Zhongguo sheng tai xue xue hui, Zhongguo ke xue yuan Shenyang ying yong sheng tai yan jiu suo zhu ban 05/2000; 11(2):228-30.
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ABSTRACT: The growth curves of engineered strain JM105(pASN) were different in LB and M-3 media. The expression level and activity of L-asparaginase were affected apparently by both biomass and induction time. Glucose repression of production of L-asparaginase was found. The stability of the recombinant plasmid pASN in different host strains and in LB and M-3 media was determined. After cultivation inLB broth and M-3 media at 30 degrees C for more than 50 generations without antibiotic selection, then induced at 42 degrees C for up to 5 h, the engineered strains were proved to be stable, except for DHA alpha (pASN).
ACTA MICROBIOLOGICA SINICA 01/2000; 39(6):546-50.
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ABSTRACT: Our recent morphologic studies indicated that peripheral nervous system (PNS) adrenergic neurons synthesize, transport, and store the serene protease, tissue plasminogen activator (t-PA) in axon terminals, many of which innervate vessel walls. Sympathoadrenal stimulation induces a surge of t-PA from vessel walls into the blood. The vascular endothelium, which constitutively secretes t-PA into blood also has long been widely assumed to be the principal source of this stress-induced release, but has not been verified as such. A neurologically regulated release from adrenergic stores could thus augment the known constitutive endothelial release. To functionally test this possibility, we quantitated the effects of guanethidine-induced systemic sympathectomy on the basal and stimulated release of t-PA from isolated vessel explants in superfused organ cultures. Moment-to-moment changes in the release rate were plotted from serial assays of the t-PA free activity. The effects of endothelial and adventitial nerve plexus ablations were also tested. Sympathectomy induced 30-50% reductions in t-PA release from both arterial and microvascular explants. An acute release induced by alpha-1 adrenergic receptor stimulations was also strongly suppressed, as were basal levels of the circulating enzyme in vivo. Adventitial and endothelial ablations from normal large vessel explants produced greater reductions than small vessel endothelial ablations. Ganglion electrical stimulation also induced an acute microvascular release in vivo. These and past morphologic findings indicate a physiological infusion of t-PA into the vessel walls, blood, and other innervated matrices by sympathetic neurons.
Journal of Neuroscience Research 10/1999; 57(5):680-92. · 2.74 Impact Factor
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ABSTRACT: To find a new way for gene therapy against tumors with weak immunogenicity, the effect of mB7-1 costimulation alone, or combined with IL-6, in inducing antitumor immunity in vitro was investigated. It was found that mB7-1 cD-NA transfected B16 cells (B16-mB7-1) induced the expansion of effector lymphocytes and the generation of specific lytic activity more effectively than wild type B16 melanoma cells (B16-wt) or mock-transfected B16 cells (B16-neo) did. (P < 0.01), IL-6 could effectively stimulate lymphocytes proliferation, but failed to enhance its cytotoxicity, while the combination of mB7-1 and IL-6 increased both lymphocyte proliferative response and T-cell-mediated cytotoxicity more significantly than B7-1 or IL-6 did alone (P < 0.01). It was inferred that the costimulatory molecule B7-1 is required for the activation and proliferation of T lymphocytes; the expression of mB7-1 in tumor cells could increase their immunogenicity and induce effective antitumor immune response, and the combination of B7-1 and IL-6 could induce more effective antitumor immunity, indicating that cooperation of IL-6 and mB7-1 plays a role in T lymphocyte activation.
Journal of Tongji Medical University = Tong ji yi ke da xue xue bao 02/1999; 19(2):86-9.
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ABSTRACT: Mouse B7-1 cDNA was cloned by RT-PCR from BALB/C mouse splenic cells and inserted into pcDNA3 to construct an eukaryotic expression vector. This constructor was named pCD-mB7-1, in which the B7-1 cDNA was identified to be consistent with the data from other researchers. pCD-mB7-1 plasmid was transfected into B16(F0) cells, and effective expression of mB7-1 in these tumor cells could be detected till the 6th month by RT-PCR and RNA hybridization. Specific cytotoxity assay of lymphocytes was conducted after culturing with tumor cells and the results demonstrated that B16 cells transfected with B7-1 gene were more effective than B16-wt and B16-neo in inducing specific cytotoxity of lymphocytes against B16-wt cells. It is suggested that expression of B7-1 gene in tumor cells could enhance the immunogenicity and induce the effective antitumor immunity.
Journal of Tongji Medical University = Tong ji yi ke da xue xue bao 02/1999; 19(1):10-4.
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Investigative Ophthalmology & Visual Science 09/1998; 39(9):1763-4. · 3.60 Impact Factor
