Tatsuyuki Yoshida

Nippon Veterinary and Life Science University, Edo, Tōkyō, Japan

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Publications (15)9.89 Total impact

  • [Show abstract] [Hide abstract]
    ABSTRACT: This study was designed to evaluate the effect of estrus synchronization treatments on recipient heifers for embryo transfer (ET). Holstein heifers were separated into the following three groups: (i) an administration of 50 µg GnRH (gonadotropin-releasing hormone) analog was given to heifers at a random stage of the estrus cycle, followed 7 days later by two administrations of 7.5 mg prostaglandin F2 alfa analog (PG) as control; (ii) another administration of 100 µg GnRH was given to the control group at 48 h after the administration of PG as the second GnRH group; and (iii) an administration of 0.75 mg estradiol benzoate (E2) was given to the control group at 24 h after the administration of PG as the E2 group. Each method caused estrus synchronization. Fresh embryos were nonsurgically transferred into the suitable recipients that had a functional corpora lutea (CL) 7 days after estrus. The E2 group showed a significantly higher (P < 0.01) rate of estrus synchronization (98.9%) at 1-3 days after PG administration and the final pregnancy rate of the E2 group (50.6%) was also significantly higher than the other groups (37.1%, P < 0.05 and 30.9%, P < 0.01, respectively). These findings demonstrate that E2 administration 24 h after PG protocol is effective for estrus synchronization of Holstein heifers, thus improving the productivity of ET.
    Animal Science Journal 07/2012; 83(7):529-34. · 1.04 Impact Factor
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    ABSTRACT: In this study, 714 cows from 26 dairy herds were reclassified as healthy or mastitic cows on the basis of long-term somatic cell count (SCC) in milk. Cows with more than three consecutive lactation records of SCC from the first or second to fifth lactation, were selected, and their BoLA-DRB3 (DRB3) alleles were identified using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. Cows with an SCC of < 200 000 cells/mL in all monthly records were classified as healthy (n=91). Cows with an SCC of > 300 000 cells/mL in two consecutive tests or four non-consecutive tests or cows with an SCC of > 500 000 cells/mL in any one test during lactation, regardless of parity, were classified as mastitic (n=201). Mastitic cows (n=153) from another 40 herds were considered to be infected if bacteriological testing revealed mastitis pathogens in milk. Their DRB3 alleles were identified using PCR-sequence-based typing (PCR-SBT). The differences in DRB3 allelic frequencies between healthy cows and cows with various degrees of mastitis were re-investigated. Moreover, the associations of various amino acid motifs in DRB3 alleles with resistance or susceptibility to mastitis pathogens were re-examined. DRB3.2*8(DRB3*1201) and DRB3.2*16(DRB3*1501) alleles were found to be associated with susceptibility, while DRB3.2*22(DRB3*1101), DRB3.2*23(DRB3*2703), and DRB3.2*24(DRB3*0101) alleles were found to be associated with resistance.
    Animal Science Journal 05/2012; 83(5):359-66. · 1.04 Impact Factor
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    ABSTRACT: Diagnosis of corpus luteum (CL) function by rectal palpation (RP) has been widely used for recipient selection of embryo transfer (ET), a technology essential for genetic improvements in cattle. To examine the accuracy of RP diagnosis method, the relationship between RP-based CL function and reproductive performance was compared in this study. In Experiment 1, CL of Holstein heifers on day 7 after estrus was classified into functional or hypoplastic by RP, and the results were compared with ultrasonographic (US) images and plasma progesterone (P4) levels. As a result, heifers with functional CL judged by RP had a mean maximum CL diameter of 20.1 ± 3.1 mm on US and a mean P4 concentration of 8.1 ± 2.3 ng/mL. These values were significantly greater than those of heifers with hypoplastic CL (12.4 ± 5.4 mm, 4.0 ± 2.8 ng/mL) (P < 0.001). In Experiment 2, the length of the estrus cycle was examined between functional CL and hypoplastic CL. The rate of heifers with a normal estrus cycle length with 18-25 days was significantly lower with hypoplastic CL than with functional CL (16/24 vs. 43/46, P < 0.01). In Experiment 3, 543 inseminated heifers were similarly classified by CL function by RP 7 days after estrus. The heifers with functional CL showed higher pregnancy rate compared with the heifers with hypoplastic CL (75.2 vs. 47.9%, P < 0.0001). Finally, the CL function of 66 heifers was examined by RP on day 7 post-estrus, and ET was performed in 49 (74.2%) heifers with functional CL. As a result, 27 (55.1%) of them became pregnant. Taken together, these results reconfirm that RP on day 7 after estrus is useful for selection of heifers with functional CL.
    Animal Science Journal 03/2012; 83(3):207-12. · 1.04 Impact Factor
  • Journal of Pet Animal Nutrition. 01/2010; 13(1):7-10.
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    ABSTRACT: The association of the polymorphism of bovine leukocyte antigen (BoLA-DRB3) genes identified by the polymerase chain reaction sequence-based typing (PCR-SBT) method with resistance and susceptibility to mastitis caused by pathogenic bacteria was investigated. Blood samples for DNA extraction were collected from 194 Holstein cows (41 healthy cows and 153 mastitis cows including 24 mixed-infection cows infected with 2 or 3 species of pathogens) from 5 districts of Chiba prefecture, Japan. Sixteen BoLA-DRB3 alleles were detected. The 4 main alleles of DRB3*0101, *1501, *1201, and *1101 constituted 56.8% of the total number of alleles detected. Mastitis cows were divided into 2 groups: group 1 with single-infection cows and group 2 with all mastitis cows including 24 mixed-infection cows. The differences in the frequencies of BoLA-DRB3 alleles and the number of cows homozygous or heterozygous for each BoLA-DRB3 allele between healthy cows and the 2 groups of mastitis cows were evaluated. Furthermore, similar comparisons were performed between healthy cows and the 2 groups of mastitis cows for each mastitis pathogen. It was considered that the 4 alleles, namely, DRB3*0101, *1501, *1201, and *1101 had specific resistance and susceptibility to 4 different mastitis pathogens. Thus, DRB3*0101 might be associated with susceptibility to coagulase-negative Staphylococci and Escherichia coli, and DRB3*1501 might be associated with susceptibility to Escherichia coli. However, DRB3*1101 might be associated with resistance to Streptococci and coagulase-negative Staphylococci, and DRB3*1201, with resistance to Streptococci, Escherichia coli, and Staphylococcus aureus.
    Animal Science Journal 10/2009; 80(5):498-509. · 1.04 Impact Factor
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    ABSTRACT: The association of the polymorphism of bovine leukocyte antigen (BoLA-DRB3) genes, identified by the polymerase chain reaction sequence-based typing (PCR-SBT) method, with resistance and susceptibility to mastitis caused by Streptococci, coagulase-negative Staphylococci, Escherichia coli and Staphylococcus aureus was investigated. Blood samples for DNA extraction were collected from 170 Holstein cows (129 mastitis and 41 healthy cows) from 5 districts in Chiba prefecture, Japan. Susceptibility or resistance to the mastitis-causing pathogens was thought to vary by the presence of amino acid substitutions at the 9, 11, 13, and 30 positions. DRB3*0101 and DRB3*1501 had amino acid motifs of Glu(9), Ser(11), Ser(13), and Tyr(30), and they were considered to have susceptibility to all 4 mastitis pathogens. In contrast, DRB3*1101 and DRB3*1401 had amino acid motifs of Gln(9), His(11), Gly(13), and His(30) in these positions, and they also had Val(86), so these alleles were considered to have resistance to Streptococcal and coagulase-negative Staphylococcal mastitis. However, in the case of Escherichia coli mastitis, amino acid substitutions at the 9, 11, 13, and 30 positions had little effect, but rather substitutions at the 47, 67 positions of pocket 7, and at the 71, 74 positions of pocket 4, Tyr(47), Ile(67), Ala(71), and Ala(74), were associated with resistance. This motif was present in DRB3*1201.
    Animal Science Journal 10/2009; 80(5):510-9. · 1.04 Impact Factor
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    ABSTRACT: Germline chimeric chickens were produced by the transfer of primordial germ cells (PGCs) or blastoderm cells. The hatchability of eggs produced by transfer of exogenous PGCs is usually low. The purpose of the present study was investigated to express (3-hydroxyacyl CoA dehydrogenase) 3HADH which is a limiting enzyme in the beta-oxidation of fatty acids for hatching energy. Manipulations of both donor and recipient eggshells were as follows. A window approximately 10 mm in diameter was opened at the pointed end of the eggs at stage 12-15 days incubation. Donor PGCs, taken from the blood vessels of donor embryos from fertilized eggs at the same stage of development, were injected into the blood vessels of recipient embryos. The muscles of chicks in the eggs with transferred PGCs were removed after 20 days of incubation. A cDNA was prepared from the total RNA. The expression of 3HADH in the manipulated embryos was investigated using real-time PCR analysis. Real-time PCR analysis showed that expression of 3HADH was reduced in the muscles of manipulated embryos.
    Cytotechnology 10/2009; 60(1-3):165-8. · 1.32 Impact Factor
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    ABSTRACT: Holstein Cows (n = 702) from 26 dairy herds in the Tama area of Tokyo, Japan were examined for polymorphisms of the BoLA-DRB3 allele using a PCR-RFLP method. Twenty alleles were observed and allelic frequencies ranged from < 1% to 20.3%. Nine alleles (DRB3.2*24,*16,*8,*23,*22,*3,*11, *10 and *7 in order) constituted 90.0% of all alleles. Somatic cell counts (SCC) were used to classify healthy (group 1), mastitis (group 2) and suspected (group 3) cows. Frequencies of DRB3.2*11 and DRB3.2*23 were slightly higher in group 1 than in group 2, whereas, frequencies of DRB3.2*8 and DRB3.2*16 were slightly higher in group 2 than in group 1. However, none of the differences in frequencies between the two groups were statistically significant. For combinations of alleles, frequencies of DRB3.2*8/*23 (P < 0.1) and DRB3.2*16/*24 (P < 0.05) were significantly higher in group 2 than in group 1, and their odds ratios were 2.1, 2.5, respectively. However, there were no significant differences between genotypes in their effects for SCC. On the other hand, frequency of DRB3.2*23/*23 including combinations of DRB3.2*23 with minor alleles was significantly higher in group 1 than in group 2 (P < 0.01), and the odds ratio was 0.3. Therefore, it was considered that mastitis resistance or susceptibility of cows may vary with the combination of BoLA-DRB3 alleles.
    Animal Science Journal 07/2008; 79(4):409 - 416. · 1.04 Impact Factor
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    ABSTRACT: The present study was carried out to investigate development of recipient chicken embryonic reproductive tracts which are transferred chicken primordial germ cells (PGCs). It is thought that differentiation of PGCs is affected by the gonadal somatic cells. When female PGCs are transferred to male embryos, it is possible that they differentiate to W-spermatogonia. However, the relationship development between PGCs and gonads has not been investigated. At stage 12-15 of incubation of fertilized eggs, donor PGCs, which were taken from the blood vessels of donor embryos, were injected into the blood vessels of recipient embryos. The gonads were removed from embryos that died after 16 days of incubation and from newly hatched chickens and organs were examined for morphological and histological features. The survival rate of the treated embryos was 13.6% for homo-sexual transfer of PGCs (male PGCs to male embryo or female PGCs to female embryo) and 28.9% for hetero-sexual transfer PGCs (male PGCs to female embryo or female PGCs to male embryo) when determined at 15 days of incubation. The gonads of embryos arising from homo-sexual transfer appeared to develop normally. In contrast, embryos derived from hetero-sexual transfer of PGCs had abnormal gonads as assessed by histological observation. These results suggest that hetero-sexual transfer of PGCs may influence gonadal development early-stage embryos.
    Cytotechnology 02/2008; 56(1):27-32. · 1.32 Impact Factor
  • Yoshiyuki OHTA, Tatsuyuki YOSHIDA, Teru ISHIBASHI
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    ABSTRACT: Four experiments were conducted to estimate dietary lysine (Lys) requirements using plasma amino acid concentrations as a criterion in mature thoroughbreds. In each experiment four adult thoroughbreds were used. The changes in plasma Lys concentration after feeding were observed in Experiment 1. Blood samples were taken from the cervix vein 0.5 h before, and 1, 3, 5.5, and 10 h after feeding. The plasma Lys concentration increased and remained constant until 3 h after feeding, decreased until 5.5 h and remained constant after then. Therefore, the bleed was done at 3 h after feeding in all later experiments. To make sure of the response speed of the plasma Lys to changes of dietary Lys levels, dietary Lys levels were changed from high to low, and low to high levels in Experiment 2. Blood samples were taken just on the changing day and 1, 2 and 3 days after changing diets. The plasma Lys concentration decreased until 2 days after changing the diet, and then remained constant with advancing days after changing the diet from high Lys to low Lys. On the other hand, the plasma Lys increased until one day after changing the diet, and then remained constant with advancing days after changing dietary Lys levels. Thus, blood samples were taken 3 days after feeding in the next experiments. The possibility of estimating the Lys requirement for maintenance using plasma Lys concentration was elucidated by two methods in Experiments 3 and 4. In Experiment 3, the horses were fed a diet containing 0.33 percent Lys for 3 days. After this the diet was changed to diets containing higher levels of Lys to 0.40, 0.47, 0.54, and 0.61 percent every third day. The Lys requirement was estimated to be 0.46 percent of diet from the response of plasma Lys concentration of five Lys levels. In Experiment 4, a 4 × 4 Latin square design was used for four dietary Lys levels. The Lys requirement was estimated to be 0.47 percent of the diet with a plasma Lys concentration of four Lys levels.
    Animal Science Journal 01/2007; 78(1):41 - 46. · 1.04 Impact Factor
  • Journal of Poultry Science - J POULT SCI. 01/2007; 44(3):335-338.
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    ABSTRACT: Furuta, H., Ichikawa, E., Sugimura, S., Kikuchi, S., Yoshida, T., Mukouyama, H. and Tomogane, H. 2006. Gene transfer to mouse embryos by sperm mediated gene transfer method. J. Appl. Anim. Res., 29: 113–116.To obtain transgenic mouse embryos by in vitro fertilization transgenic mouse sperm were produced by electroporation using green fluorescent protein (GFP)gene as a marker. The sperm was cultured in medium GFP gene. Electoroporation was carried out under 200 V-1μF, 200V-25μF and 200V-50μF. The survival rate of sperm decreased by each treatment. Although the DNA transfer into sperm mediated by electroporation was very easy, efficiency of introduction into embryos was very low. Sperm mediated gene transfer may be inferior to microinjection.
    Journal of Applied Animal Research - J APPL ANIM RES. 01/2006; 29(2):113-116.
  • Journal of Equine Science. 01/2002; 13(1):23-27.
  • Journal of Poultry Science - J POULT SCI. 01/2002; 39(3):194-197.
  • Journal of Equine Science. 01/2002; 13(1):19-22.